LOCUS       pBRsE6Hy3               5507 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 2966.
SOURCE      
  ORGANISM  
COMMENT     VNTNAME|pBRsE6Hy3|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pBRsE6Hy3" 0 5507 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 5507 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
COMMENT        #3=(CFSignal (CObList) "tac" 30 0 0 32 65 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "32..65") 
COMMENT        #4=(CFSignal (CObList) "5UTR" 21 0 0 66 128 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "66..128") 
COMMENT        #5=(CFSignal (CObList) "sPHOA" 21 0 0 129 191 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "129..191") 
COMMENT        #6=(CFSignal (CObList) "VH" 4 0 0 192 551 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "192..551") 
COMMENT        #7=(CFSignal (CObList) "CH1" 4 0 0 552 842 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "552..842") 
COMMENT        #8=(CFSignal (CObList) "H1-4" 4 0 0 843 1028 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "843..1028") 
COMMENT        #9=(CFSignal (CObList) "CH2" 4 0 0 1029 1361 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1029..1361") 
COMMENT        #10=(CFSignal (CObList) "CH3" 4 0 0 1362 1682 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1362..1682") 
COMMENT        #11=(CFSignal (CObList) "3UTR" 21 0 0 1683 1799 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1683..1799") 
COMMENT        #12=(CFSignal (CObList) "MOUSE" 21 0 0 192 731 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "192..731") 
COMMENT        #13=(CFSignal (CObList) "HUMAN" 21 0 0 732 1799 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "732..1799") 
COMMENT        #14=(CFSignal (CObList) "ORI" 33 0 1 3500 3900 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3500..3900)") 
COMMENT        #15=(CFSignal (CObList) "AMP" 4 0 1 4439 5299 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(4439..5299)")) (CObList) 
COMMENT       (CObList) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CTextView 0 
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COMMENT                          "DNA 'pBRsE6Hy3'" 1) 
COMMENT                     #20=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Foreign object. Author: UNKNOWN. Original author: BCCM/GeneCorner Plasmid Collection" 
COMMENT                          1) 
COMMENT                     #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Creation Date: ? (no data)" 1) 
COMMENT                     #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modification Date: ? (no data)" 1) 
COMMENT                     #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5507 bp" 1) 
COMMENT                     #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                    (CObjectList 
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COMMENT                          "Original Source Database: GenBank" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                   "Annotations") 
COMMENT              #30=(CFolderPar 
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COMMENT                    (CObjectList 
COMMENT                     #31=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #32=(CFolderPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #33=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 192   End: 551 " 1) 
COMMENT                                   #34=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "variable domain of E6H" 1) 
COMMENT                                   #35=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #36=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   192..551" 1))) "VH") 
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COMMENT                                        "Start: 552   End: 842 " 1) 
COMMENT                                   #39=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "chimeric constant domain 1 of the heavy chain" 
COMMENT                                        1) 
COMMENT                                   #40=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
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COMMENT                                        "   552..842" 1))) "CH1") 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 843   End: 1028" 1) 
COMMENT                                   #44=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "hinge domain of HIG3" 1) 
COMMENT                                   #45=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #46=(CLinePar 
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COMMENT                                        "   843..1028" 1))) "H1-4") 
COMMENT                            #47=(CFolderPar 
COMMENT                                 (CGroupPar 
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COMMENT                                   #48=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1029  End: 1361" 1) 
COMMENT                                   #49=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "constant domain 2 of the human \3543 heavy chain" 
COMMENT                                        1) 
COMMENT                                   #50=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #51=(CLinePar 
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COMMENT                            #52=(CFolderPar 
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COMMENT                                   #53=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1362  End: 1682" 1) 
COMMENT                                   #54=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "constant domain 3 of the human \3543 heavy chain" 
COMMENT                                        1) 
COMMENT                                   #55=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4439  End: 5299 (Complementary)" 
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COMMENT                                   #59=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "antibiotic resistance gene; coding sequence" 
COMMENT                                        1) 
COMMENT                                   #60=(CLinePar 
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COMMENT                                   #61=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(4439..5299)" 1))) "AMP"))) 
COMMENT                          "CDS (6 total)") 
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COMMENT                                   #64=(CLinePar 
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COMMENT                                        "Start: 66    End: 128 " 1) 
COMMENT                                   #65=(CLinePar 
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COMMENT                                        "5' UTR containing the RBS of the E. coli phoA gene" 
COMMENT                                        1) 
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COMMENT                                        "   66..128" 1))) "5UTR") 
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COMMENT                                        "Start: 129   End: 191 " 1) 
COMMENT                                   #70=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "E. coli alkaline phosphatase A signal sequence" 
COMMENT                                        1) 
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COMMENT                                   #74=(CLinePar 
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COMMENT                                        "Start: 192   End: 731 " 1) 
COMMENT                                   #75=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "mouse part of E6Hy3" 1) 
COMMENT                                   #76=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #77=(CLinePar 
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COMMENT                                   #80=(CLinePar 
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COMMENT                          "Misc. Feature (5 total)") 
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COMMENT                                        "Start: 32    End: 65  " 1) 
COMMENT                                   #91=(CLinePar 
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COMMENT                                        "E. coli hybrid tryptophan/lac operon promoter" 
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COMMENT                          "Promoter Prokaryotic (1 total)") 
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COMMENT                                        "Start: 3500  End: 3900 (Complementary)" 
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COMMENT                                   #97=(CLinePar 
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COMMENT                                        "   complement(3500..3900)" 1))) "ORI"))) 
COMMENT                          "Replication Origin (1 total)"))) "Feature Map")))) 
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COMMENT                "@F bp (molecule @L bp)" 0) 
COMMENT          #106=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
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FEATURES             Location/Qualifiers
     promoter        32..65
                     /vntifkey="30"
                     /label=tac
                     /note="E. coli hybrid tryptophan/lac operon promoter"
     misc_feature    66..128
                     /vntifkey="21"
                     /label=5UTR
                     /note="5' UTR containing the RBS of the E. coli phoA gene"
     misc_feature    129..191
                     /vntifkey="21"
                     /label=sPHOA
                     /note="E. coli alkaline phosphatase A signal sequence"
     CDS             192..551
                     /vntifkey="4"
                     /label=VH
                     /note="variable domain of E6H"
     CDS             552..842
                     /vntifkey="4"
                     /label=CH1
                     /note="chimeric constant domain 1 of the heavy chain"
     CDS             843..1028
                     /vntifkey="4"
                     /label=H1-4
                     /note="hinge domain of HIG3"
     CDS             1029..1361
                     /vntifkey="4"
                     /label=CH2
                     /note="constant domain 2 of the human ì3 heavy chain"
     CDS             1362..1682
                     /vntifkey="4"
                     /label=CH3
                     /note="constant domain 3 of the human ì3 heavy chain"
     misc_feature    1683..1799
                     /vntifkey="21"
                     /label=3UTR
                     /note="3' UTR of HIG3"
     misc_feature    192..731
                     /vntifkey="21"
                     /label=MOUSE
                     /note="mouse part of E6Hy3"
     misc_feature    732..1799
                     /vntifkey="21"
                     /label=HUMAN
                     /note="human part of E6Hy3"
     rep_origin      complement(3500..3900)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     CDS             complement(4439..5299)
                     /vntifkey="4"
                     /label=AMP
                     /note="antibiotic resistance gene; coding sequence"
BASE COUNT     1300 a      1579 c      1409 g      1219 t 
ORIGIN
        1 ttcgagctcg agcttactcc ccatccccct gttgacaatt aatcatcggc tcgtataatg 
       61 tgtggaattg tgagcggata acaatttcac acaggaaaca ggatccgcgg atccgtggag 
      121 aaaataaagt gaaacaaagc actattgcac tggcactctt accgttactg tttacccctg 
      181 tgacaaaagc gcaggttcag ctgcagcagt ctggagctga gctggcgagg cctggggcct 
      241 cagtgaagct gtcctgcaag gcctctggat acaccctcac aagttatggt ataagttggg 
      301 tgaagcagag aactggacag ggccttgagt ggattggaga aatttatcct ggaagtggaa 
      361 attcgtactt caatgagaag tttaagggca aggccacact gactgtagac aaatcctcca 
      421 gcacagccta cttgcacctc agcagcctga catctgagga ctctgccgtc tatttctgtg 
      481 ccggacctag acaggtcggg ctactccctt ttggttactg gggccaaggg actctggtca 
      541 ctgcctctgc agccaaaaca acacccccat cagtctatcc actggcccct gggtgtggag 
      601 atacaactgg ttcctccgtg actctgggat gcctggtcaa gggctacttc cctgagtcag 
      661 tgactgtgac ttggaactcc ggatccctgt ccagcagtgt gcacaccttc ccagctctcc 
      721 tgcagtctgg actctactcc ctcagcagcg tggtgaccgt gccctccagc agcttgggca 
      781 cccagaccta cacctgcaac gtgaatcaca agcccagcaa caccaaggtg gacaagagag 
      841 ttgagctcaa aaccccactt ggtgacacaa ctcacacatg cccacggtgc ccagagccca 
      901 aatcttgtga cacacctccc ccgtgcccac ggtgcccaga gcccaaatct tgtgacacac 
      961 ctcccccatg cccacggtgc ccagagccca aatcttgtga cacacctccc ccgtgcccaa 
     1021 ggtgcccagc acctgaactc ttgggaggac cgtcagtctt cctcttcccc ccaaaaccca 
     1081 aggataccct tatgatttcc cggacccctg aggtcacgtg cgtggtggtg gacgtgagcc 
     1141 acgaagaccc cgaggtccag ttcaagtggt acgtggacgg cgtggaggtg cataatgcca 
     1201 agacaaagct gcgggaggag cagtacaaca gcacgttccg tgtggtcagc gtcctcaccg 
     1261 tcctgcacca ggactggctg aacggcaagg agtacaagtg caaggtctcc aacaaagccc 
     1321 tcccagcccc catcgagaaa accatctcca aagccaaagg acagccccga gaaccacagg 
     1381 tgtacaccct gcccccatcc cgggaggaga tgaccaagaa ccaagtcagc ctgacctgcc 
     1441 tggtcaaagg cttctacccc agcgacatcg ccgtggagtg ggagagcaat gggcagccgg 
     1501 agaacaacta caacaccacg cctcccatgc tggactccga cggctccttc ttcctctaca 
     1561 gcaagctcac cgtggacaag agcaggtggc agcaggggaa catcttctca tgctccgtga 
     1621 tgcatgaggc tctgcacaac cgcttcacgc agaagagcct ctccctgtct ccgggtaaat 
     1681 gagtgcgaca gccggcaagc ccccgctccc cgggctctcg gggtcgcgcg aggatgcttg 
     1741 gcacgtaccc cgtgtacata cttcccgggc acccagcatg gaaataaagc acccaggtcg 
     1801 accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac 
     1861 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc 
     1921 agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct 
     1981 gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc 
     2041 caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg 
     2101 ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct 
     2161 cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga 
     2221 cgaccatcag ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcac 
     2281 tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc 
     2341 atggattgta ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg 
     2401 gagccgggcc acctcgacct gaatggaagc cggcggcacc tcgctaacgg attcaccact 
     2461 ccaagaattg gagccaatca attcttgcgg agaactgtga atgcgcaaac caacccttgg 
     2521 cagaacatat ccatcgcgtc cgccatctcc agcagccgca cgcggcgcat ctcgggcagc 
     2581 gttgggtcct ggccacgggt gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc 
     2641 tggcggggtt gccttactgg ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc 
     2701 gactgctgct gcaaaacgtc tgcgacctga gcaacaacat gaatggtctt cggtttccgt 
     2761 gtttcgtaaa gtctggaaac gcggaagtca gcgccctgca ccattatgtt ccggatctgc 
     2821 atcgcaggat gctgctggct accctgtgga acacctacat ctgtattaac gaagcgctgg 
     2881 cattgaccct gagtgatttt tctctggtcc cgccgcatcc ataccgccag ttgtttaccc 
     2941 tcacaacgtt ccagtaaccg ggcatgttca tcatcagtaa cccgtatcgt gagcatcctc 
     3001 tctcgtttca tcggtatcat tacccccatg aacagaaatc ccccttacac ggaggcatca 
     3061 gtgaccaaac aggaaaaaac cgcccttaac atggcccgct ttatcagaag ccagacatta 
     3121 acgcttctgg agaaactcaa cgagctggac gcggatgaac aggcagacat ctgtgaatcg 
     3181 cttcacgacc acgctgatga gctttaccgc agctgcctcg cgcgtttcgg tgatgacggt 
     3241 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc 
     3301 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc 
     3361 atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc 
     3421 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa 
     3481 aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 
     3541 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 
     3601 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 
     3661 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 
     3721 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 
     3781 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 
     3841 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 
     3901 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 
     3961 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 
     4021 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 
     4081 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 
     4141 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 
     4201 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 
     4261 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 
     4321 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 
     4381 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 
     4441 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 
     4501 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 
     4561 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 
     4621 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 
     4681 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 
     4741 ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 
     4801 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 
     4861 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 
     4921 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 
     4981 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 
     5041 cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca 
     5101 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 
     5161 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 
     5221 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 
     5281 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 
     5341 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 
     5401 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat 
     5461 taacctataa aaataggcgt atcacgaggc cctttcgtct tcaagaa 
//
