LOCUS       pBRsE6Hy3f2sE6L         5845 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 2967.
SOURCE      
  ORGANISM  
COMMENT     VNTNAME|pBRsE6Hy3f2sE6L|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pBRsE6Hy3f2sE6L" 0 5845 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 5845 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
COMMENT        #3=(CFSignal (CObList) "tac" 30 0 0 32 65 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "32..65") 
COMMENT        #4=(CFSignal (CObList) "5UTR" 21 0 0 66 128 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "66..128") 
COMMENT        #5=(CFSignal (CObList) "sPHOA" 21 0 0 129 191 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "129..191") 
COMMENT        #6=(CFSignal (CObList) "VH" 4 0 0 192 551 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "192..551") 
COMMENT        #7=(CFSignal (CObList) "CH1" 4 0 0 552 842 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "552..842") 
COMMENT        #8=(CFSignal (CObList) "H1-4" 4 0 0 843 1028 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "843..1028") 
COMMENT        #9=(CFSignal (CObList) "CH2f" 4 0 0 1029 1124 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1029..1124") 
COMMENT        #10=(CFSignal (CObList) "MOUSE" 21 0 0 192 731 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "192..731") 
COMMENT        #11=(CFSignal (CObList) "HUMAN" 21 0 0 732 1124 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "732..1124") 
COMMENT        #12=(CFSignal (CObList) "tac" 30 0 0 1163 1197 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1163..1197") 
COMMENT        #13=(CFSignal (CObList) "5UTR" 21 0 0 1198 1260 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1198..1260") 
COMMENT        #14=(CFSignal (CObList) "sPHOA" 21 0 0 1261 1323 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1261..1323") 
COMMENT        #15=(CFSignal (CObList) "VL" 4 0 0 1324 1644 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1324..1644") 
COMMENT        #16=(CFSignal (CObList) "CL" 4 0 0 1645 1968 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1645..1968") 
COMMENT        #17=(CFSignal (CObList) "3UTR" 21 0 0 1969 2137 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1969..2137") 
COMMENT        #18=(CFSignal (CObList) "MOUSE" 21 0 0 1324 2137 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1324..2137") 
COMMENT        #19=(CFSignal (CObList) "ORI" 33 0 1 3835 4235 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3835..4235)") 
COMMENT        #20=(CFSignal (CObList) "AMP" 4 0 1 4787 5637 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(4787..5637)")) (CObList) 
COMMENT       (CObList) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #21=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
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COMMENT              #22=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) "pBRsE6Hy3f2sE6L" 
COMMENT                    2) 5 "" 0 4) 
COMMENT              #23=(CFolderPar 
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COMMENT                          "DNA 'pBRsE6Hy3f2sE6L'" 1) 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Foreign object. Author: UNKNOWN. Original author: BCCM/GeneCorner Plasmid Collection" 
COMMENT                          1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Creation Date: ? (no data)" 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modification Date: ? (no data)" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5845 bp" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #31=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #33=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #34=(CFolderPar 
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COMMENT                   "Annotations") 
COMMENT              #35=(CFolderPar 
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COMMENT                    (CObjectList 
COMMENT                     #36=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #37=(CFolderPar 
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COMMENT                                   #38=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 192   End: 551 " 1) 
COMMENT                                   #39=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "variable domain of E6H" 1) 
COMMENT                                   #40=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #41=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   192..551" 1))) "VH") 
COMMENT                            #42=(CFolderPar 
COMMENT                                 (CGroupPar 
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COMMENT                                   #43=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 552   End: 842 " 1) 
COMMENT                                   #44=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "chimeric constant domain 1 of the heavy chain" 
COMMENT                                        1) 
COMMENT                                   #45=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #46=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   552..842" 1))) "CH1") 
COMMENT                            #47=(CFolderPar 
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COMMENT                                  (CParagraph 843 (3 #8# 0) 1 2 2 0 327) 
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COMMENT                                   #48=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 843   End: 1028" 1) 
COMMENT                                   #49=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "hinge domain of human \3543" 1) 
COMMENT                                   #50=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #51=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   843..1028" 1))) "H1-4") 
COMMENT                            #52=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1029 (3 #9# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #53=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1029  End: 1124" 1) 
COMMENT                                   #54=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "fragment of CH2 of the human \3543 heavy chain" 
COMMENT                                        1) 
COMMENT                                   #55=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #56=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1029..1124" 1))) "CH2f") 
COMMENT                            #57=(CFolderPar 
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COMMENT                                   #58=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1324  End: 1644" 1) 
COMMENT                                   #59=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "variable domain of E6L" 1) 
COMMENT                                   #60=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #61=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1324..1644" 1))) "VL") 
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COMMENT                                   #63=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1645  End: 1968" 1) 
COMMENT                                   #64=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "constant domain of E6L" 1) 
COMMENT                                   #65=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #66=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1645..1968" 1))) "CL") 
COMMENT                            #67=(CFolderPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #68=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4787  End: 5637 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #69=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "antibiotic resistance gene; coding sequence" 
COMMENT                                        1) 
COMMENT                                   #70=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #71=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(4787..5637)" 1))) "AMP"))) 
COMMENT                          "CDS (7 total)") 
COMMENT                     #72=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #73=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 66 (3 #4# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #74=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 66    End: 128 " 1) 
COMMENT                                   #75=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "5' UTR containing the RBS of the E. coli phoA gene" 
COMMENT                                        1) 
COMMENT                                   #76=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   66..128" 1))) "5UTR") 
COMMENT                            #78=(CFolderPar 
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COMMENT                                  (CParagraph 129 (3 #5# 0) 1 2 2 0 194) 
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COMMENT                                   #79=(CLinePar 
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COMMENT                                        "Start: 129   End: 191 " 1) 
COMMENT                                   #80=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "E. coli alkaline phosphatase A signal sequence" 
COMMENT                                        1) 
COMMENT                                   #81=(CLinePar 
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COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   129..191" 1))) "sPHOA") 
COMMENT                            #83=(CFolderPar 
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COMMENT                                   #84=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 192   End: 731 " 1) 
COMMENT                                   #85=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "mouse part of E6Hy3f2" 1) 
COMMENT                                   #86=(CLinePar 
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COMMENT                                   #87=(CLinePar 
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COMMENT                                        "   192..731" 1))) "MOUSE") 
COMMENT                            #88=(CFolderPar 
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COMMENT                                   #89=(CLinePar 
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COMMENT                                        "Start: 732   End: 1124" 1) 
COMMENT                                   #90=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "human part of E6Hy3f2" 1) 
COMMENT                                   #91=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #92=(CLinePar 
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COMMENT                                        "   732..1124" 1))) "HUMAN") 
COMMENT                            #93=(CFolderPar 
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COMMENT                                   #94=(CLinePar 
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COMMENT                                        "Start: 1198  End: 1260" 1) 
COMMENT                                   #95=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "5' UTR containing the RBS of the E. coli phoA gene" 
COMMENT                                        1) 
COMMENT                                   #96=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #97=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1198..1260" 1))) "5UTR") 
COMMENT                            #98=(CFolderPar 
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COMMENT                                   #99=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1261  End: 1323" 1) 
COMMENT                                   #100=(CLinePar 
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COMMENT                                         "E. coli alkaline phosphatase A signal sequence" 
COMMENT                                         1) 
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COMMENT                                   #102=(CLinePar 
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COMMENT                                         "   1261..1323" 1))) "sPHOA") 
COMMENT                            #103=(CFolderPar 
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COMMENT                                    #104=(CLinePar 
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COMMENT                                          "Start: 1324  End: 2137" 1) 
COMMENT                                    #105=(CLinePar 
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COMMENT                                          "mouse E6L" 1) 
COMMENT                                    #106=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Original Location Description:" 1) 
COMMENT                                    #107=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "   1324..2137" 1))) "MOUSE") 
COMMENT                            #108=(CFolderPar 
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COMMENT                                   (CParagraph 1969 (3 #17# 0) 1 2 2 0 194) 
COMMENT                                   (CObjectList 
COMMENT                                    #109=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Start: 1969  End: 2137" 1) 
COMMENT                                    #110=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "3' UTR 0F E6L" 1) 
COMMENT                                    #111=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Original Location Description:" 1) 
COMMENT                                    #112=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "   1969..2137" 1))) "3UTR"))) 
COMMENT                          "Misc. Feature (8 total)") 
COMMENT                     #113=(CFolderPar 
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COMMENT                            (CObjectList 
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COMMENT                                     #115=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 32    End: 65  " 1) 
COMMENT                                     #116=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "E. coli hybrid tryptophan/lac operon promoter" 
COMMENT                                           1) 
COMMENT                                     #117=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #118=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   32..65" 1))) "tac") 
COMMENT                             #119=(CFolderPar 
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COMMENT                                     #120=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 1163  End: 1197" 1) 
COMMENT                                     #121=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "E. coli hybrid tryptophan/lac operon promoter" 
COMMENT                                           1) 
COMMENT                                     #122=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
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FEATURES             Location/Qualifiers
     promoter        32..65
                     /vntifkey="30"
                     /label=tac
                     /note="E. coli hybrid tryptophan/lac operon promoter"
     misc_feature    66..128
                     /vntifkey="21"
                     /label=5UTR
                     /note="5' UTR containing the RBS of the E. coli phoA gene"
     misc_feature    129..191
                     /vntifkey="21"
                     /label=sPHOA
                     /note="E. coli alkaline phosphatase A signal sequence"
     CDS             192..551
                     /vntifkey="4"
                     /label=VH
                     /note="variable domain of E6H"
     CDS             552..842
                     /vntifkey="4"
                     /label=CH1
                     /note="chimeric constant domain 1 of the heavy chain"
     CDS             843..1028
                     /vntifkey="4"
                     /label=H1-4
                     /note="hinge domain of human ì3"
     CDS             1029..1124
                     /vntifkey="4"
                     /label=CH2f
                     /note="fragment of CH2 of the human ì3 heavy chain"
     misc_feature    192..731
                     /vntifkey="21"
                     /label=MOUSE
                     /note="mouse part of E6Hy3f2"
     misc_feature    732..1124
                     /vntifkey="21"
                     /label=HUMAN
                     /note="human part of E6Hy3f2"
     promoter        1163..1197
                     /vntifkey="30"
                     /label=tac
                     /note="E. coli hybrid tryptophan/lac operon promoter"
     misc_feature    1198..1260
                     /vntifkey="21"
                     /label=5UTR
                     /note="5' UTR containing the RBS of the E. coli phoA gene"
     misc_feature    1261..1323
                     /vntifkey="21"
                     /label=sPHOA
                     /note="E. coli alkaline phosphatase A signal sequence"
     CDS             1324..1644
                     /vntifkey="4"
                     /label=VL
                     /note="variable domain of E6L"
     CDS             1645..1968
                     /vntifkey="4"
                     /label=CL
                     /note="constant domain of E6L"
     misc_feature    1969..2137
                     /vntifkey="21"
                     /label=3UTR
                     /note="3' UTR 0F E6L"
     misc_feature    1324..2137
                     /vntifkey="21"
                     /label=MOUSE
                     /note="mouse E6L"
     rep_origin      complement(3835..4235)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     CDS             complement(4787..5637)
                     /vntifkey="4"
                     /label=AMP
                     /note="antibiotic resistance gene; coding sequence"
BASE COUNT     1413 a      1644 c      1429 g      1359 t 
ORIGIN
        1 ttcgagctcg agcttactcc ccatccccct gttgacaatt aatcatcggc tcgtataatg 
       61 tgtggaattg tgagcggata acaatttcac acaggaaaca ggatccgcgg atccgtggag 
      121 aaaataaagt gaaacaaagc actattgcac tggcactctt accgttactg tttacccctg 
      181 tgacaaaagc gcaggttcag ctgcagcagt ctggagctga gctggcgagg cctggggcct 
      241 cagtgaagct gtcctgcaag gcctctggat acaccctcac aagttatggt ataagttggg 
      301 tgaagcagag aactggacag ggccttgagt ggattggaga aatttatcct ggaagtggaa 
      361 attcgtactt caatgagaag tttaagggca aggccacact gactgtagac aaatcctcca 
      421 gcacagccta cttgcacctc agcagcctga catctgagga ctctgccgtc tatttctgtg 
      481 ccggacctag acaggtcggg ctactccctt ttggttactg gggccaaggg actctggtca 
      541 ctgcctctgc agccaaaaca acacccccat cagtctatcc actggcccct gggtgtggag 
      601 atacaactgg ttcctccgtg actctgggat gcctggtcaa gggctacttc cctgagtcag 
      661 tgactgtgac ttggaactcc ggatccctgt ccagcagtgt gcacaccttc ccagctctcc 
      721 tgcagtctgg actctactcc ctcagcagcg tggtgaccgt gccctccagc agcttgggca 
      781 cccagaccta cacctgcaac gtgaatcaca agcccagcaa caccaaggtg gacaagagag 
      841 ttgagctcaa aaccccactt ggtgacacaa ctcacacatg cccacggtgc ccagagccca 
      901 aatcttgtga cacacctccc ccgtgcccac ggtgcccaga gcccaaatct tgtgacacac 
      961 ctcccccatg cccacggtgc ccagagccca aatcttgtga cacacctccc ccgtgcccaa 
     1021 ggtgcccagc acctgaactc ttgggaggac cgtcagtctt cctcttcccc ccaaaaccca 
     1081 aggataccct tatgatttcc cggacccctg acttagttaa ctaagcttag ttaactaagt 
     1141 cgagcttact ccccatcccc ctgttgacaa ttaatcatcg gctcgtataa tgtgtggaat 
     1201 tgtgagcgga taacaatttc acacaggaaa caggatccgc ggatccgtgg agaaaataaa 
     1261 gtgaaacaaa gcactattgc actggcactc ttaccgttac tgtttacccc tgtgacaaaa 
     1321 gcggacatcc agatgactca gtctccagcc tccctatccg tttctgtggg agaatctgtc 
     1381 accatcacat gtcgagcaag tgagaatatt tacagtaatt tagcatggta tcagcagaaa 
     1441 cagggaaaat ctcctcagct ccttgtctat gttgcgacaa agttagtaga tggtgtgcca 
     1501 tcaaggttca gtggcagtgg atcaggcaca cagtattccc tcaagatcaa cagcctgcag 
     1561 tctgaagatt ttgggagtta ttactgtcaa catttttggg acactccatt cacgttcggc 
     1621 tcggggacaa agttggaaat gaaacgggct gatgctgcac caactgtatc catcttccca 
     1681 ccatccagtg agcagttaac atctggaggt gcctcagtcg tgtgcttctt gaacaacttc 
     1741 taccccaaag acatcaatgt caagtggaag attgatggga gtgaacgaca aaatggcgtc 
     1801 ctgaacagtt ggactgatca ggacagcaaa gacagcacct acagcatgag cagcaccctc 
     1861 acgttgacca aggacgagta tgaacgacat aacagctata cctgtgaggc cactcacaag 
     1921 acatcaactt cacccattgt caagagcttc aacaggaatg agtgttagag acaaaggtcc 
     1981 tgagacgcca ccaccagctc cccagctcca tcctatcttc ccttctaagg tcttggaggc 
     2041 ttccccacaa gcgacctacc actgttgcgg tgctccaaac ctcctcccca cctccttctc 
     2101 ctcctcctcc ctttccttcc cccccccccc ccccgtcgac cgatgccctt gagagccttc 
     2161 aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact 
     2221 gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc 
     2281 gaggaccgct ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc 
     2341 ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag 
     2401 caggccatta tcgccggcat ggcggccgac gcgctgggct acgtcttgct ggcgttcgcg 
     2461 acgcgaggct ggatggcctt ccccattatg attcttctcg cttccggcgg catcgggatg 
     2521 cccgcgttgc aggccatgct gtccaggcag gtagatgacg accatcaggg acagcttcaa 
     2581 ggatcgctcg cggctcttac cagcctaact tcgatcactg gaccgctgat cgtcacggcg 
     2641 atttatgccg cctcggcgag cacatggaac gggttggcat ggattgtagg cgccgcccta 
     2701 taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac ctcgacctga 
     2761 atggaagccg gcggcacctc gctaacggat tcaccactcc aagaattgga gccaatcaat 
     2821 tcttgcggag aactgtgaat gcgcaaacca acccttggca gaacatatcc atcgcgtccg 
     2881 ccatctccag cagccgcacg cggcgcatct cgggcagcgt tgggtcctgg ccacgggtgc 
     2941 gcatgatcgt gctcctgtcg ttgaggaccc ggctaggctg gcggggttgc cttactggtt 
     3001 agcagaatga atcaccgata cgcgagcgaa cgtgaagcga ctgctgctgc aaaacgtctg 
     3061 cgacctgagc aacaacatga atggtcttcg gtttccgtgt ttcgtaaagt ctggaaacgc 
     3121 ggaagtcagc gccctgcacc attatgttcc ggatctgcat cgcaggatgc tgctggctac 
     3181 cctgtggaac acctacatct gtattaacga agcgctggca ttgaccctga gtgatttttc 
     3241 tctggtcccg ccgcatccat accgccagtt gtttaccctc acaacgttcc agtaaccggg 
     3301 catgttcatc atcagtaacc cgtatcgtga gcatcctctc tcgtttcatc ggtatcatta 
     3361 cccccatgaa cagaaatccc ccttacacgg aggcatcagt gaccaaacag gaaaaaaccg 
     3421 cccttaacat ggcccgcttt atcagaagcc agacattaac gcttctggag aaactcaacg 
     3481 agctggacgc ggatgaacag gcagacatct gtgaatcgct tcacgaccac gctgatgagc 
     3541 tttaccgcag ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc 
     3601 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg 
     3661 gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata 
     3721 gcggagtgta tactggctta actatgcggc atcagagcag attgtactga gagtgcacca 
     3781 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc 
     3841 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 
     3901 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 
     3961 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 
     4021 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 
     4081 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 
     4141 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 
     4201 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 
     4261 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 
     4321 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 
     4381 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 
     4441 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt 
     4501 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 
     4561 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 
     4621 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 
     4681 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 
     4741 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc 
     4801 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta 
     4861 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga 
     4921 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg 
     4981 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc 
     5041 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat 
     5101 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag 
     5161 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat 
     5221 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa 
     5281 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa 
     5341 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga 
     5401 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg 
     5461 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc 
     5521 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg 
     5581 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact 
     5641 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat 
     5701 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 
     5761 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat 
     5821 cacgaggccc tttcgtcttc aagaa 
//
