LOCUS       pBRsE6Hy3sE6L           6503 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 2968.
SOURCE      
  ORGANISM  
COMMENT     VNTNAME|pBRsE6Hy3sE6L|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pBRsE6Hy3sE6L" 0 6503 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 6503 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
COMMENT        #3=(CFSignal (CObList) "tac" 30 0 0 32 65 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "32..65") 
COMMENT        #4=(CFSignal (CObList) "5UTR" 21 0 0 66 128 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "66..128") 
COMMENT        #5=(CFSignal (CObList) "sPHOA" 21 0 0 129 191 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "129..191") 
COMMENT        #6=(CFSignal (CObList) "VH" 4 0 0 192 551 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "192..551") 
COMMENT        #7=(CFSignal (CObList) "CH1" 4 0 0 552 842 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "552..842") 
COMMENT        #8=(CFSignal (CObList) "H1-4" 4 0 0 843 1028 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "843..1028") 
COMMENT        #9=(CFSignal (CObList) "CH2" 4 0 0 1029 1361 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1029..1361") 
COMMENT        #10=(CFSignal (CObList) "CH3" 4 0 0 1362 1682 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1362..1682") 
COMMENT        #11=(CFSignal (CObList) "3UTR" 21 0 0 1683 1799 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1683..1799") 
COMMENT        #12=(CFSignal (CObList) "MOUSE" 21 0 0 192 731 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "192..731") 
COMMENT        #13=(CFSignal (CObList) "HUMAN" 21 0 0 732 1799 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "732..1799") 
COMMENT        #14=(CFSignal (CObList) "tac" 30 0 0 1821 1855 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1821..1855") 
COMMENT        #15=(CFSignal (CObList) "5UTR" 21 0 0 1856 1918 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1856..1918") 
COMMENT        #16=(CFSignal (CObList) "sPHOA" 21 0 0 1919 1981 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1919..1981") 
COMMENT        #17=(CFSignal (CObList) "VL" 4 0 0 1982 2302 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1982..2302") 
COMMENT        #18=(CFSignal (CObList) "CL" 4 0 0 2303 2626 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2303..2626") 
COMMENT        #19=(CFSignal (CObList) "3UTR" 21 0 0 2627 2795 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2627..2795") 
COMMENT        #20=(CFSignal (CObList) "MOUSE" 21 0 0 1982 2795 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1982..2795") 
COMMENT        #21=(CFSignal (CObList) "ORI" 33 0 1 4495 4895 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(4495..4895)") 
COMMENT        #22=(CFSignal (CObList) "AMP" 4 0 1 5435 6295 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(5435..6295)")) (CObList) 
COMMENT       (CObList) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CTextView 0 
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COMMENT                   5 "" 0 4) 
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COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA 'pBRsE6Hy3sE6L'" 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Foreign object. Author: UNKNOWN. Original author: BCCM/GeneCorner Plasmid Collection" 
COMMENT                          1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Creation Date: ? (no data)" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modification Date: ? (no data)" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6503 bp" 1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #33=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #35=(CRefLinePar 
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COMMENT                   0 0) 
COMMENT              #36=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) 
COMMENT                   "Annotations") 
COMMENT              #37=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #38=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #39=(CFolderPar 
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COMMENT                                  (CParagraph 192 (3 #6# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #40=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 192   End: 551 " 1) 
COMMENT                                   #41=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "variable domain of E6H" 1) 
COMMENT                                   #42=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #43=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   192..551" 1))) "VH") 
COMMENT                            #44=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 552 (3 #7# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #45=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 552   End: 842 " 1) 
COMMENT                                   #46=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "chimeric constant domain 1 of the heavy chain" 
COMMENT                                        1) 
COMMENT                                   #47=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #48=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   552..842" 1))) "CH1") 
COMMENT                            #49=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 843 (3 #8# 0) 1 2 2 0 327) 
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COMMENT                                   #50=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 843   End: 1028" 1) 
COMMENT                                   #51=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "hinge domain of human \3543" 1) 
COMMENT                                   #52=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #53=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   843..1028" 1))) "H1-4") 
COMMENT                            #54=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1029 (3 #9# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #55=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1029  End: 1361" 1) 
COMMENT                                   #56=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "constant domain 2 of the human \3543 heavy chain" 
COMMENT                                        1) 
COMMENT                                   #57=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #58=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1029..1361" 1))) "CH2") 
COMMENT                            #59=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1362 (3 #10# 0) 1 2 2 0 327) 
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COMMENT                                   #60=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1362  End: 1682" 1) 
COMMENT                                   #61=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "constant domain 3 of the human \3543 heavy chain" 
COMMENT                                        1) 
COMMENT                                   #62=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #63=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1362..1682" 1))) "CH3") 
COMMENT                            #64=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1982 (3 #17# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #65=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1982  End: 2302" 1) 
COMMENT                                   #66=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "variable domain of E6L" 1) 
COMMENT                                   #67=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #68=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1982..2302" 1))) "VL") 
COMMENT                            #69=(CFolderPar 
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COMMENT                                  (CParagraph 2303 (3 #18# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #70=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2303  End: 2626" 1) 
COMMENT                                   #71=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "constant domain of E6L" 1) 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2303..2626" 1))) "CL") 
COMMENT                            #74=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5435 (3 #22# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #75=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5435  End: 6295 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #76=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "antibiotic resistance gene; coding sequence" 
COMMENT                                        1) 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #78=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(5435..6295)" 1))) "AMP"))) 
COMMENT                          "CDS (8 total)") 
COMMENT                     #79=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
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COMMENT                                   #81=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 66    End: 128 " 1) 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "5' UTR containing the RBS of the E. coli phoA gene" 
COMMENT                                        1) 
COMMENT                                   #83=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #84=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   66..128" 1))) "5UTR") 
COMMENT                            #85=(CFolderPar 
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COMMENT                                  (CParagraph 129 (3 #5# 0) 1 2 2 0 194) 
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COMMENT                                   #86=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 129   End: 191 " 1) 
COMMENT                                   #87=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "E. coli alkaline phosphatase A signal sequence" 
COMMENT                                        1) 
COMMENT                                   #88=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #89=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   129..191" 1))) "sPHOA") 
COMMENT                            #90=(CFolderPar 
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COMMENT                                   #91=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 192   End: 731 " 1) 
COMMENT                                   #92=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "mouse part of E6Hy3" 1) 
COMMENT                                   #93=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #94=(CLinePar 
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COMMENT                                        "   192..731" 1))) "MOUSE") 
COMMENT                            #95=(CFolderPar 
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COMMENT                                   #96=(CLinePar 
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COMMENT                                        "Start: 732   End: 1799" 1) 
COMMENT                                   #97=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "human part of E6Hy3" 1) 
COMMENT                                   #98=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #99=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   732..1799" 1))) "HUMAN") 
COMMENT                            #100=(CFolderPar 
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COMMENT                                    #102=(CLinePar 
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COMMENT                                          "3' UTR of the human \3543 heavy chain" 
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COMMENT                                    #103=(CLinePar 
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COMMENT                                    #104=(CLinePar 
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COMMENT                                          "   1683..1799" 1))) "3UTR") 
COMMENT                            #105=(CFolderPar 
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COMMENT                                          "Start: 1856  End: 1918" 1) 
COMMENT                                    #107=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "5' UTR containing the RBS of the E. coli phoA gene" 
COMMENT                                          1) 
COMMENT                                    #108=(CLinePar 
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COMMENT                                          "Original Location Description:" 1) 
COMMENT                                    #109=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "   1856..1918" 1))) "5UTR") 
COMMENT                            #110=(CFolderPar 
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COMMENT                                   (CParagraph 1919 (3 #16# 0) 1 2 2 0 194) 
COMMENT                                   (CObjectList 
COMMENT                                    #111=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Start: 1919  End: 1981" 1) 
COMMENT                                    #112=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "E. coli alkaline phosphatase A signal sequence" 
COMMENT                                          1) 
COMMENT                                    #113=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Original Location Description:" 1) 
COMMENT                                    #114=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "   1919..1981" 1))) "sPHOA") 
COMMENT                            #115=(CFolderPar 
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COMMENT                                    #116=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Start: 1982  End: 2795" 1) 
COMMENT                                    #117=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "mouse E6L" 1) 
COMMENT                                    #118=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Original Location Description:" 1) 
COMMENT                                    #119=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "   1982..2795" 1))) "MOUSE") 
COMMENT                            #120=(CFolderPar 
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COMMENT                                    #121=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Start: 2627  End: 2795" 1) 
COMMENT                                    #122=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "3' UTR of E6L" 1) 
COMMENT                                    #123=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
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FEATURES             Location/Qualifiers
     promoter        32..65
                     /vntifkey="30"
                     /label=tac
                     /note="E. coli hybrid tryptophan/lac operon promoter"
     misc_feature    66..128
                     /vntifkey="21"
                     /label=5UTR
                     /note="5' UTR containing the RBS of the E. coli phoA gene"
     misc_feature    129..191
                     /vntifkey="21"
                     /label=sPHOA
                     /note="E. coli alkaline phosphatase A signal sequence"
     CDS             192..551
                     /vntifkey="4"
                     /label=VH
                     /note="variable domain of E6H"
     CDS             552..842
                     /vntifkey="4"
                     /label=CH1
                     /note="chimeric constant domain 1 of the heavy chain"
     CDS             843..1028
                     /vntifkey="4"
                     /label=H1-4
                     /note="hinge domain of human ì3"
     CDS             1029..1361
                     /vntifkey="4"
                     /label=CH2
                     /note="constant domain 2 of the human ì3 heavy chain"
     CDS             1362..1682
                     /vntifkey="4"
                     /label=CH3
                     /note="constant domain 3 of the human ì3 heavy chain"
     misc_feature    1683..1799
                     /vntifkey="21"
                     /label=3UTR
                     /note="3' UTR of the human ì3 heavy chain"
     misc_feature    192..731
                     /vntifkey="21"
                     /label=MOUSE
                     /note="mouse part of E6Hy3"
     misc_feature    732..1799
                     /vntifkey="21"
                     /label=HUMAN
                     /note="human part of E6Hy3"
     promoter        1821..1855
                     /vntifkey="30"
                     /label=tac
                     /note="E. coli hybrid tryptophan/lac operon promoter"
     misc_feature    1856..1918
                     /vntifkey="21"
                     /label=5UTR
                     /note="5' UTR containing the RBS of the E. coli phoA gene"
     misc_feature    1919..1981
                     /vntifkey="21"
                     /label=sPHOA
                     /note="E. coli alkaline phosphatase A signal sequence"
     CDS             1982..2302
                     /vntifkey="4"
                     /label=VL
                     /note="variable domain of E6L"
     CDS             2303..2626
                     /vntifkey="4"
                     /label=CL
                     /note="constant domain of E6L"
     misc_feature    2627..2795
                     /vntifkey="21"
                     /label=3UTR
                     /note="3' UTR of E6L"
     misc_feature    1982..2795
                     /vntifkey="21"
                     /label=MOUSE
                     /note="mouse E6L"
     rep_origin      complement(4495..4895)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     CDS             complement(5435..6295)
                     /vntifkey="4"
                     /label=AMP
                     /note="antibiotic resistance gene; coding sequence"
BASE COUNT     1569 a      1861 c      1619 g      1454 t 
ORIGIN
        1 ttcgagctcg agcttactcc ccatccccct gttgacaatt aatcatcggc tcgtataatg 
       61 tgtggaattg tgagcggata acaatttcac acaggaaaca ggatccgcgg atccgtggag 
      121 aaaataaagt gaaacaaagc actattgcac tggcactctt accgttactg tttacccctg 
      181 tgacaaaagc gcaggttcag ctgcagcagt ctggagctga gctggcgagg cctggggcct 
      241 cagtgaagct gtcctgcaag gcctctggat acaccctcac aagttatggt ataagttggg 
      301 tgaagcagag aactggacag ggccttgagt ggattggaga aatttatcct ggaagtggaa 
      361 attcgtactt caatgagaag tttaagggca aggccacact gactgtagac aaatcctcca 
      421 gcacagccta cttgcacctc agcagcctga catctgagga ctctgccgtc tatttctgtg 
      481 ccggacctag acaggtcggg ctactccctt ttggttactg gggccaaggg actctggtca 
      541 ctgcctctgc agccaaaaca acacccccat cagtctatcc actggcccct gggtgtggag 
      601 atacaactgg ttcctccgtg actctgggat gcctggtcaa gggctacttc cctgagtcag 
      661 tgactgtgac ttggaactcc ggatccctgt ccagcagtgt gcacaccttc ccagctctcc 
      721 tgcagtctgg actctactcc ctcagcagcg tggtgaccgt gccctccagc agcttgggca 
      781 cccagaccta cacctgcaac gtgaatcaca agcccagcaa caccaaggtg gacaagagag 
      841 ttgagctcaa aaccccactt ggtgacacaa ctcacacatg cccacggtgc ccagagccca 
      901 aatcttgtga cacacctccc ccgtgcccac ggtgcccaga gcccaaatct tgtgacacac 
      961 ctcccccatg cccacggtgc ccagagccca aatcttgtga cacacctccc ccgtgcccaa 
     1021 ggtgcccagc acctgaactc ttgggaggac cgtcagtctt cctcttcccc ccaaaaccca 
     1081 aggataccct tatgatttcc cggacccctg aggtcacgtg cgtggtggtg gacgtgagcc 
     1141 acgaagaccc cgaggtccag ttcaagtggt acgtggacgg cgtggaggtg cataatgcca 
     1201 agacaaagct gcgggaggag cagtacaaca gcacgttccg tgtggtcagc gtcctcaccg 
     1261 tcctgcacca ggactggctg aacggcaagg agtacaagtg caaggtctcc aacaaagccc 
     1321 tcccagcccc catcgagaaa accatctcca aagccaaagg acagccccga gaaccacagg 
     1381 tgtacaccct gcccccatcc cgggaggaga tgaccaagaa ccaagtcagc ctgacctgcc 
     1441 tggtcaaagg cttctacccc agcgacatcg ccgtggagtg ggagagcaat gggcagccgg 
     1501 agaacaacta caacaccacg cctcccatgc tggactccga cggctccttc ttcctctaca 
     1561 gcaagctcac cgtggacaag agcaggtggc agcaggggaa catcttctca tgctccgtga 
     1621 tgcatgaggc tctgcacaac cgcttcacgc agaagagcct ctccctgtct ccgggtaaat 
     1681 gagtgcgaca gccggcaagc ccccgctccc cgggctctcg gggtcgcgcg aggatgcttg 
     1741 gcacgtaccc cgtgtacata cttcccgggc acccagcatg gaaataaagc acccaggtcg 
     1801 agcttactcc ccatccccct gttgacaatt aatcatcggc tcgtataatg tgtggaattg 
     1861 tgagcggata acaatttcac acaggaaaca ggatccgcgg atccgtggag aaaataaagt 
     1921 gaaacaaagc actattgcac tggcactctt accgttactg tttacccctg tgacaaaagc 
     1981 ggacatccag atgactcagt ctccagcctc cctatccgtt tctgtgggag aatctgtcac 
     2041 catcacatgt cgagcaagtg agaatattta cagtaattta gcatggtatc agcagaaaca 
     2101 gggaaaatct cctcagctcc ttgtctatgt tgcgacaaag ttagtagatg gtgtgccatc 
     2161 aaggttcagt ggcagtggat caggcacaca gtattccctc aagatcaaca gcctgcagtc 
     2221 tgaagatttt gggagttatt actgtcaaca tttttgggac actccattca cgttcggctc 
     2281 ggggacaaag ttggaaatga aacgggctga tgctgcacca actgtatcca tcttcccacc 
     2341 atccagtgag cagttaacat ctggaggtgc ctcagtcgtg tgcttcttga acaacttcta 
     2401 ccccaaagac atcaatgtca agtggaagat tgatgggagt gaacgacaaa atggcgtcct 
     2461 gaacagttgg actgatcagg acagcaaaga cagcacctac agcatgagca gcaccctcac 
     2521 gttgaccaag gacgagtatg aacgacataa cagctatacc tgtgaggcca ctcacaagac 
     2581 atcaacttca cccattgtca agagcttcaa caggaatgag tgttagagac aaaggtcctg 
     2641 agacgccacc accagctccc cagctccatc ctatcttccc ttctaaggtc ttggaggctt 
     2701 ccccacaagc gacctaccac tgttgcggtg ctccaaacct cctccccacc tccttctcct 
     2761 cctcctccct ttccttcccc cccccccccc ccgtcgaccg atgcccttga gagccttcaa 
     2821 cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt 
     2881 cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca ttttcggcga 
     2941 ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat tcggaatctt 
     3001 gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca 
     3061 ggccattatc gccggcatgg cggccgacgc gctgggctac gtcttgctgg cgttcgcgac 
     3121 gcgaggctgg atggccttcc ccattatgat tcttctcgct tccggcggca tcgggatgcc 
     3181 cgcgttgcag gccatgctgt ccaggcaggt agatgacgac catcagggac agcttcaagg 
     3241 atcgctcgcg gctcttacca gcctaacttc gatcactgga ccgctgatcg tcacggcgat 
     3301 ttatgccgcc tcggcgagca catggaacgg gttggcatgg attgtaggcg ccgccctata 
     3361 ccttgtctgc ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct cgacctgaat 
     3421 ggaagccggc ggcacctcgc taacggattc accactccaa gaattggagc caatcaattc 
     3481 ttgcggagaa ctgtgaatgc gcaaaccaac ccttggcaga acatatccat cgcgtccgcc 
     3541 atctccagca gccgcacgcg gcgcatctcg ggcagcgttg ggtcctggcc acgggtgcgc 
     3601 atgatcgtgc tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct tactggttag 
     3661 cagaatgaat caccgatacg cgagcgaacg tgaagcgact gctgctgcaa aacgtctgcg 
     3721 acctgagcaa caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct ggaaacgcgg 
     3781 aagtcagcgc cctgcaccat tatgttccgg atctgcatcg caggatgctg ctggctaccc 
     3841 tgtggaacac ctacatctgt attaacgaag cgctggcatt gaccctgagt gatttttctc 
     3901 tggtcccgcc gcatccatac cgccagttgt ttaccctcac aacgttccag taaccgggca 
     3961 tgttcatcat cagtaacccg tatcgtgagc atcctctctc gtttcatcgg tatcattacc 
     4021 cccatgaaca gaaatccccc ttacacggag gcatcagtga ccaaacagga aaaaaccgcc 
     4081 cttaacatgg cccgctttat cagaagccag acattaacgc ttctggagaa actcaacgag 
     4141 ctggacgcgg atgaacaggc agacatctgt gaatcgcttc acgaccacgc tgatgagctt 
     4201 taccgcagct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc 
     4261 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc 
     4321 gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc 
     4381 ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata 
     4441 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg 
     4501 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 
     4561 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 
     4621 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 
     4681 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 
     4741 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 
     4801 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 
     4861 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 
     4921 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 
     4981 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 
     5041 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 
     5101 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 
     5161 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 
     5221 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 
     5281 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 
     5341 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 
     5401 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 
     5461 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 
     5521 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 
     5581 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 
     5641 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 
     5701 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg 
     5761 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 
     5821 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 
     5881 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 
     5941 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 
     6001 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata 
     6061 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 
     6121 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 
     6181 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 
     6241 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 
     6301 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 
     6361 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 
     6421 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca 
     6481 cgaggccctt tcgtcttcaa gaa 
//
