LOCUS       pBRgHIL61               9484 bp    DNA     circular     29-AUG-2018
DEFINITION  LMBP 2989.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 2989
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 29-AUG-2018
            
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDBDATE|-12442301|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pBRgHIL61|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT        #2=(CRSite (CStringList) "XbaI" "tctaga" 2 0 2 1275 0 3017 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 2487 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "SphI" "gcatgc" 6 0 3 633 0 2956 0 5272 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "StyI" "ccwwgg" 2 0 6 312 0 331 0 1297 0 1729 0 
COMMENT            3790 0 6075 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 3489 0 9483 0 0 "")) 
COMMENT       (CObList 
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COMMENT        #8=(CFSignal (CObList) "hIL6 promoter" 29 0 0 1942 2477 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1942..2477") 
COMMENT        #9=(CFSignal (CObList) "5' UTR of hIL6" 21 0 0 2478 2540 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "2478..2540") 
COMMENT        #10=(CFSignal (CObList) "hIL6 exon 1" 4 0 0 2541 2559 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2541..2559") 
COMMENT        #11=(CFSignal (CObList) "hIL6 intron 1" 15 0 0 2560 2721 0 (CStringList) 
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COMMENT        #12=(CFSignal (CObList) "hIL6 exon 2" 4 0 0 2722 2912 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2722..2912") 
COMMENT        #13=(CFSignal (CObList) "hIL6 intron 2 fragment" 15 0 0 2913 3490 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "2913..3490") 
COMMENT        #14=(CFSignal (CObList) "tetracycline resistance" 4 0 0 4791 5981 0 
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COMMENT        #15=(CFSignal (CObList) "pMB1 ori" 33 0 1 7055 7457 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(7055..7457)") 
COMMENT        #16=(CFSignal (CObList) "ampicillin resistance" 4 0 1 7998 8858 0 
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COMMENT                          "TEL: 0032-9-33-13-843  " 1) 
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FEATURES             Location/Qualifiers
     misc_feature    1..1941
                     /vntifkey="21"
                     /label=5'\flanking\region\of\hIL6
     promoter        1942..2477
                     /vntifkey="29"
                     /label=hIL6\promoter
     misc_feature    2478..2540
                     /vntifkey="21"
                     /label=5'\UTR\of\hIL6
     CDS             2541..2559
                     /vntifkey="4"
                     /label=hIL6\exon\1
     intron          2560..2721
                     /vntifkey="15"
                     /label=hIL6\intron\1
     CDS             2722..2912
                     /vntifkey="4"
                     /label=hIL6\exon\2
     intron          2913..3490
                     /vntifkey="15"
                     /label=hIL6\intron\2\fragment
     CDS             4791..5981
                     /vntifkey="4"
                     /label=tetracycline\resistance
     rep_origin      complement(7055..7457)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(7998..8858)
                     /vntifkey="4"
                     /label=ampicillin\resistance
BASE COUNT     2359 a      2425 c      2408 g      2292 t 
ORIGIN
        1 ttctgacata aaaacactta gtggagggct tggaaaaagt ctagtaggag caagacgcaa 
       61 gctggactaa ttatctaaaa caagagacct ggtttgggga tcttaatgtt ctcaaaaaag 
      121 aaaattatta ttatttttca ttttgcactt tgtgccataa aacattttca acaaaacata 
      181 gaatctcatt tcttttgagg gaaaatgatt gggagaccag ctcattgctg gcacagaggc 
      241 ctggttcatt cataattcct tcataggcaa gacaccaggt gaaccgatat agccgagctg 
      301 gaagagctct ccaaggcaga gactctgagc caaggaatgt tcaaagagct agcatgtatt 
      361 gtgggattac tatgcgccag gaatttttta cactgcatca cgttccatct tcacaacagc 
      421 cctagaaagg aagaactatt attacccccg ttttataggt gaataaacaa gggcacaggt 
      481 ccttgatgta acagccagga tcaaacagct gggaagacga gaaaaccttt cccaggctag 
      541 gataacagag gatttggttg aaaatacagg caattaggtg ctacctctgg gaaaaggggc 
      601 caggagagga aggagacact tttccctgca tgccctgatg tcctatttga acattttatc 
      661 atgaacacga acttcctatt taaaaaacac tttttattga aaagataaat ctgtgtgttg 
      721 tattgtgtca ctcagttcaa gtacttgaaa tttattgaat tgtattttct aaaaaataga 
      781 tagttgagta aaagcaagct cacattacat agacggatca cagtgcacgg ctgcggagct 
      841 gggagcagtg gcttcgtttc atgcaggaaa gagaacttgg ttcaggagtg tctacgttgc 
      901 ttaagacagg agagcactaa aaatgaaacc atccagccat cctcccccat tttcattttc 
      961 acaccaaaga atcccaccgc ggcagaggac caccgtctct gtttagacaa tcggtgaaga 
     1021 atggatgacc tcactttccc caacaggcgg gtcctgaaat gttatgcacg aaacaaaact 
     1081 tgagtaaatg cccaacagag gtcactgttt tatcgatctt gaagagatct cttcttagca 
     1141 aagcaaagaa accgattgtg aaggtaacac catgtttggt aaataagtgt tttggtgttg 
     1201 tgcaagggtc tggtttcagc ctgaagccat ctcagagctg tctgggtctc tggagactgg 
     1261 agggacaacc tagtctagag cccatttgca tgagaccaag gatcctcctg caagagacac 
     1321 catcctgagg gaagagggct tctgaaccag cttgacccaa taagaaattc ttgggtgccg 
     1381 acgcggaagc agattcagag cctagagccg tgcctgcgtc cgtagtttcc ttctagcttc 
     1441 ttttgatttc aaatcaagac ttacagggag agggagcgat aaacacaaac tctgcaagat 
     1501 gccacaaggt cctcctttga catccccaac aaagaggtga gtagtattct ccccctttct 
     1561 gccctgaacc aagtgggctt cagtaatttc agggctccag gagacctggg gcccatgcag 
     1621 gtgccccagt gaaacagtgg tgaagagact cagtggcaat ggggagagca ctggcagcac 
     1681 aaggcaaacc tctggcacag agagcaaagt cctcactggg aggattccca aggggtcact 
     1741 tgggagaggg cagggcagca gccaacctcc tctaagtggg ctgaagcagg tgaagaaagt 
     1801 ggcagaagcc acgcggtggc aaaaaggagt cacacactcc acctggagac gccttgaagt 
     1861 aactgcacga aatttgagga tggccaggca gttctacaac agccgctcac agggagagcc 
     1921 agaacacaga agaactcaga tgactggtag tattaccttc ttcataatcc caggcttggg 
     1981 gggctgcgat ggagtcagag gaaactcagt tcagaacatc tttggttttt acaaatacaa 
     2041 attaactgga acgctaaatt ctagcctgtt aatctggtca ctgaaaaaaa attttttttt 
     2101 tttcaaaaaa catagcttta gcttattttt tttctctttg taaaacttcg tgcatgactt 
     2161 cagctttact ctttgtcaag acatgccaaa gtgctgagtc actaataaaa gaaaaaaaga 
     2221 aagtaaagga agagtggttc tgcttcttag cgctagcctc aatgacgacc taagctgcac 
     2281 ttttccccct agttgtgtct tgcgatgcta aaggacgtca cattgcacaa tcttaataag 
     2341 gtttccaatc agccccaccc gctctggccc caccctcacc ctccaacaaa gatttatcaa 
     2401 atgtgggatt ttcccatgag tctcaatatt agagtctcaa cccccaataa atataggact 
     2461 ggagatgtct gaggctcatt ctgccctcga gcccaccggg aacgaaagag aagctctatc 
     2521 tcccctccag gagcccagct atgaactcct tctccacaag taagtgcagg aaatccttag 
     2581 ccctggaact gccagcggcg gtcgagccct gtgtgaggga ggggtgtgtg gcccagggag 
     2641 ggctggcggg cggccagcag cagaggcagg ctcccagctg tgctgtcagc tcacccctgc 
     2701 gctcgctccc ctccggcaca ggcgccttcg gtccagttgc cttctccctg gggctgctcc 
     2761 tggtgttgcc tgctgccttc cctgccccag tacccccagg agaagattcc aaagatgtag 
     2821 ccgccccaca cagacagcca ctcacctctt cagaacgaat tgacaaacaa attcggtaca 
     2881 tcctcgacgg catctcagcc ctgagaaagg aggtgggtag gcttggcgat ggggttgaag 
     2941 ggcccggtgc gcatgcgttc cccttgcccc tgcgtgtggc cgggggctgc ctgcattagg 
     3001 aggtctttgc tgggttctag agcactgtag atttgaggcc aacggggccg actagactga 
     3061 cttctgtatt tatcctttgc tggtgtcagg aagttccttt cctttctgga aaatgcagaa 
     3121 tgggtctgaa atccatgccc acctttggca tgagctgagg gttattgctt ctcagggctt 
     3181 ccttttccct ttccaaaaaa ttaggtctgt gaagctcctt tttgtccccc gggctttgga 
     3241 aggactagaa aagtgccacc tgaaaggcat gttcagcttc tcagagcagt tgcagtactt 
     3301 tttggttatg taaactcaat ggctaggatt cctcaaagcc attccagcta agattcatac 
     3361 ctcagagccc accaaagtgg caaatcataa ataggttaaa gcatctcccc actttcaatg 
     3421 caaggtattt tggtcctgtt tggtagaaag aaaagaacac aggaggggag attgggagcc 
     3481 cacactcgaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca 
     3541 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata 
     3601 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga 
     3661 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc 
     3721 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc 
     3781 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat 
     3841 tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca 
     3901 acagtactgc gatgagtggc agggcggggc gtaatttttt taaggcagtt attggtgccc 
     3961 ttaaacgcct ggtgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg 
     4021 aaagcaaatt cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct 
     4081 attgctggtt taccggttta ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg 
     4141 cctgaggcca gtttgctcag gctctccccg tggaggtaat aattgacgat atgatcattt 
     4201 attctgcctc ccagagcctg ataaaaacgg tgaatccgtt agcgaggtgc cgccggcttc 
     4261 cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag 
     4321 gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata 
     4381 aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga 
     4441 tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc 
     4501 gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg 
     4561 cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc 
     4621 ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg 
     4681 caagattccg aataccgcaa gcgacaggcc atgtttgaca gcttatcatc gataagcttt 
     4741 aatgcggtag tttatcacag ttaaattgct aacgcagtca ggcaccgtgt atgaaatcta 
     4801 acaatgcgct catcgtcatc ctcggcaccg tcaccctgga tgctgtaggc ataggcttgg 
     4861 ttatgccggt actgccgggc ctcttgcggg atatcgtcca ttccgacagc atcgccagtc 
     4921 actatggcgt gctgctagcg ctatatgcgt tgatgcaatt tctatgcgca cccgttctcg 
     4981 gagcactgtc cgaccgcttt ggccgccgcc cagtcctgct cgcttcgcta cttggagcca 
     5041 ctatcgacta cgcgatcatg gcgaccacac ccgtcctgtg gatcctctac gccggacgca 
     5101 tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca 
     5161 ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta 
     5221 tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc 
     5281 ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt 
     5341 cgcataaggg agagcgtcga ccgatgccct tgagagcctt caacccagtc agctccttcc 
     5401 ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac 
     5461 tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc tttcgctgga 
     5521 gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag 
     5581 ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt atcgccggca 
     5641 tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct 
     5701 tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg caggccatgc 
     5761 tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc gcggctctta 
     5821 ccagcctaac ttcgatcact ggaccgctga tcgtcacggc gatttatgcc gcctcggcga 
     5881 gcacatggaa cgggttggca tggattgtag gcgccgccct ataccttgtc tgcctccccg 
     5941 cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg aatggaagcc ggcggcacct 
     6001 cgctaacgga ttcaccactc caagaattgg agccaatcaa ttcttgcgga gaactgtgaa 
     6061 tgcgcaaacc aacccttggc agaacatatc catcgcgtcc gccatctcca gcagccgcac 
     6121 gcggcgcatc tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc 
     6181 gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg aatcaccgat 
     6241 acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag caacaacatg 
     6301 aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag cgccctgcac 
     6361 cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc 
     6421 tgtattaacg aagcgctggc attgaccctg agtgattttt ctctggtccc gccgcatcca 
     6481 taccgccagt tgtttaccct cacaacgttc cagtaaccgg gcatgttcat catcagtaac 
     6541 ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt acccccatga acagaaatcc 
     6601 cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt 
     6661 tatcagaagc cagacattaa cgcttctgga gaaactcaac gagctggacg cggatgaaca 
     6721 ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca gctgcctcgc 
     6781 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc 
     6841 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg 
     6901 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt 
     6961 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg 
     7021 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac 
     7081 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 
     7141 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 
     7201 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 
     7261 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 
     7321 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 
     7381 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 
     7441 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 
     7501 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 
     7561 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 
     7621 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 
     7681 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 
     7741 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 
     7801 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 
     7861 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 
     7921 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 
     7981 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 
     8041 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 
     8101 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 
     8161 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 
     8221 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 
     8281 gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg 
     8341 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 
     8401 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 
     8461 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 
     8521 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 
     8581 atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc 
     8641 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 
     8701 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 
     8761 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 
     8821 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 
     8881 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 
     8941 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa 
     9001 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt 
     9061 cgaataaata cctgtgacgg aagatcactt cgcagaataa ataaatcctg gtgtccctgt 
     9121 tgataccggg aagccctggg ccaacttttg gcgaaaatga gacgttgatc ggcacgtaag 
     9181 aggttccaac tttcaccata atgaaataag atcactaccg ggcgtatttt ttgagttatc 
     9241 gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt 
     9301 tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg 
     9361 tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa 
     9421 taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc 
     9481 ggaa 
//
