ID pBRgHIL61 standard; circular DNA; ; 9484 BP. DT 28-AUG-2018 DE LMBP 2989. CC Accession number: LMBP 2989 CC (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium) CC Last modified: 29-AUG-2018 CC CC CC This file is created by Vector NTI CC http://www.invitrogen.com/ CC ORIGDB|GenBank CC VNTDBDATE|-12442301| CC LSOWNER| CC VNTAUTHORNAME|Demo User| CC VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTOAUTHORTEL|0032-9-33-13-843| CC VNTOAUTHOREML|bccm.genecorner@UGent.be| CC VNTOAUTHORWWW|www.genecorner.ugent.be| CC VNTOAUTHORAD1|Ghent University| CC VNTOAUTHORAD2|Technologiepark 927| CC VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT misc_feature 1..1941 FT /vntifkey="21" FT /label=5'\flanking\region\of\hIL6 FT promoter 1942..2477 FT /vntifkey="29" FT /label=hIL6\promoter FT misc_feature 2478..2540 FT /vntifkey="21" FT /label=5'\UTR\of\hIL6 FT CDS 2541..2559 FT /vntifkey="4" FT /label=hIL6\exon\1 FT intron 2560..2721 FT /vntifkey="15" FT /label=hIL6\intron\1 FT CDS 2722..2912 FT /vntifkey="4" FT /label=hIL6\exon\2 FT intron 2913..3490 FT /vntifkey="15" FT /label=hIL6\intron\2\fragment FT CDS 4791..5981 FT /vntifkey="4" FT /label=tetracycline\resistance FT rep_origin complement(7055..7457) FT /vntifkey="33" FT /label=pMB1\ori FT CDS complement(7998..8858) FT /vntifkey="4" FT /label=ampicillin\resistance SQ Sequence 9484 BP; 2359 A; 2425 C; 2408 G; 2292 t; ttctgacata aaaacactta gtggagggct tggaaaaagt ctagtaggag caagacgcaa 60 gctggactaa ttatctaaaa caagagacct ggtttgggga tcttaatgtt ctcaaaaaag 120 aaaattatta ttatttttca ttttgcactt tgtgccataa aacattttca acaaaacata 180 gaatctcatt tcttttgagg gaaaatgatt gggagaccag ctcattgctg gcacagaggc 240 ctggttcatt cataattcct tcataggcaa gacaccaggt gaaccgatat agccgagctg 300 gaagagctct ccaaggcaga gactctgagc caaggaatgt tcaaagagct agcatgtatt 360 gtgggattac tatgcgccag gaatttttta cactgcatca cgttccatct tcacaacagc 420 cctagaaagg aagaactatt attacccccg ttttataggt gaataaacaa gggcacaggt 480 ccttgatgta acagccagga tcaaacagct gggaagacga gaaaaccttt cccaggctag 540 gataacagag gatttggttg aaaatacagg caattaggtg ctacctctgg gaaaaggggc 600 caggagagga aggagacact tttccctgca tgccctgatg tcctatttga acattttatc 660 atgaacacga acttcctatt taaaaaacac tttttattga aaagataaat ctgtgtgttg 720 tattgtgtca ctcagttcaa gtacttgaaa tttattgaat tgtattttct aaaaaataga 780 tagttgagta aaagcaagct cacattacat agacggatca cagtgcacgg ctgcggagct 840 gggagcagtg gcttcgtttc atgcaggaaa gagaacttgg ttcaggagtg tctacgttgc 900 ttaagacagg agagcactaa aaatgaaacc atccagccat cctcccccat tttcattttc 960 acaccaaaga atcccaccgc ggcagaggac caccgtctct gtttagacaa tcggtgaaga 1020 atggatgacc tcactttccc caacaggcgg gtcctgaaat gttatgcacg aaacaaaact 1080 tgagtaaatg cccaacagag gtcactgttt tatcgatctt gaagagatct cttcttagca 1140 aagcaaagaa accgattgtg aaggtaacac catgtttggt aaataagtgt tttggtgttg 1200 tgcaagggtc tggtttcagc ctgaagccat ctcagagctg tctgggtctc tggagactgg 1260 agggacaacc tagtctagag cccatttgca tgagaccaag gatcctcctg caagagacac 1320 catcctgagg gaagagggct tctgaaccag cttgacccaa taagaaattc ttgggtgccg 1380 acgcggaagc agattcagag cctagagccg tgcctgcgtc cgtagtttcc ttctagcttc 1440 ttttgatttc aaatcaagac ttacagggag agggagcgat aaacacaaac tctgcaagat 1500 gccacaaggt cctcctttga catccccaac aaagaggtga gtagtattct ccccctttct 1560 gccctgaacc aagtgggctt cagtaatttc agggctccag gagacctggg gcccatgcag 1620 gtgccccagt gaaacagtgg tgaagagact cagtggcaat ggggagagca ctggcagcac 1680 aaggcaaacc tctggcacag agagcaaagt cctcactggg aggattccca aggggtcact 1740 tgggagaggg cagggcagca gccaacctcc tctaagtggg ctgaagcagg tgaagaaagt 1800 ggcagaagcc acgcggtggc aaaaaggagt cacacactcc acctggagac gccttgaagt 1860 aactgcacga aatttgagga tggccaggca gttctacaac agccgctcac agggagagcc 1920 agaacacaga agaactcaga tgactggtag tattaccttc ttcataatcc caggcttggg 1980 gggctgcgat ggagtcagag gaaactcagt tcagaacatc tttggttttt acaaatacaa 2040 attaactgga acgctaaatt ctagcctgtt aatctggtca ctgaaaaaaa attttttttt 2100 tttcaaaaaa catagcttta gcttattttt tttctctttg taaaacttcg tgcatgactt 2160 cagctttact ctttgtcaag acatgccaaa gtgctgagtc actaataaaa gaaaaaaaga 2220 aagtaaagga agagtggttc tgcttcttag cgctagcctc aatgacgacc taagctgcac 2280 ttttccccct agttgtgtct tgcgatgcta aaggacgtca cattgcacaa tcttaataag 2340 gtttccaatc agccccaccc gctctggccc caccctcacc ctccaacaaa gatttatcaa 2400 atgtgggatt ttcccatgag tctcaatatt agagtctcaa cccccaataa atataggact 2460 ggagatgtct gaggctcatt ctgccctcga gcccaccggg aacgaaagag aagctctatc 2520 tcccctccag gagcccagct atgaactcct tctccacaag taagtgcagg aaatccttag 2580 ccctggaact gccagcggcg gtcgagccct gtgtgaggga ggggtgtgtg gcccagggag 2640 ggctggcggg cggccagcag cagaggcagg ctcccagctg tgctgtcagc tcacccctgc 2700 gctcgctccc ctccggcaca ggcgccttcg gtccagttgc cttctccctg gggctgctcc 2760 tggtgttgcc tgctgccttc cctgccccag tacccccagg agaagattcc aaagatgtag 2820 ccgccccaca cagacagcca ctcacctctt cagaacgaat tgacaaacaa attcggtaca 2880 tcctcgacgg catctcagcc ctgagaaagg aggtgggtag gcttggcgat ggggttgaag 2940 ggcccggtgc gcatgcgttc cccttgcccc tgcgtgtggc cgggggctgc ctgcattagg 3000 aggtctttgc tgggttctag agcactgtag atttgaggcc aacggggccg actagactga 3060 cttctgtatt tatcctttgc tggtgtcagg aagttccttt cctttctgga aaatgcagaa 3120 tgggtctgaa atccatgccc acctttggca tgagctgagg gttattgctt ctcagggctt 3180 ccttttccct ttccaaaaaa ttaggtctgt gaagctcctt tttgtccccc gggctttgga 3240 aggactagaa aagtgccacc tgaaaggcat gttcagcttc tcagagcagt tgcagtactt 3300 tttggttatg taaactcaat ggctaggatt cctcaaagcc attccagcta agattcatac 3360 ctcagagccc accaaagtgg caaatcataa ataggttaaa gcatctcccc actttcaatg 3420 caaggtattt tggtcctgtt tggtagaaag aaaagaacac aggaggggag attgggagcc 3480 cacactcgaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca 3540 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata 3600 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga 3660 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc 3720 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc 3780 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat 3840 tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca 3900 acagtactgc gatgagtggc agggcggggc gtaatttttt taaggcagtt attggtgccc 3960 ttaaacgcct ggtgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg 4020 aaagcaaatt cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct 4080 attgctggtt taccggttta ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg 4140 cctgaggcca gtttgctcag gctctccccg tggaggtaat aattgacgat atgatcattt 4200 attctgcctc ccagagcctg ataaaaacgg tgaatccgtt agcgaggtgc cgccggcttc 4260 cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag 4320 gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata 4380 aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga 4440 tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc 4500 gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg 4560 cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc 4620 ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg 4680 caagattccg aataccgcaa gcgacaggcc atgtttgaca gcttatcatc gataagcttt 4740 aatgcggtag tttatcacag ttaaattgct aacgcagtca ggcaccgtgt atgaaatcta 4800 acaatgcgct catcgtcatc ctcggcaccg tcaccctgga tgctgtaggc ataggcttgg 4860 ttatgccggt actgccgggc ctcttgcggg atatcgtcca ttccgacagc atcgccagtc 4920 actatggcgt gctgctagcg ctatatgcgt tgatgcaatt tctatgcgca cccgttctcg 4980 gagcactgtc cgaccgcttt ggccgccgcc cagtcctgct cgcttcgcta cttggagcca 5040 ctatcgacta cgcgatcatg gcgaccacac ccgtcctgtg gatcctctac gccggacgca 5100 tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca 5160 ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta 5220 tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc 5280 ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt 5340 cgcataaggg agagcgtcga ccgatgccct tgagagcctt caacccagtc agctccttcc 5400 ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac 5460 tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc tttcgctgga 5520 gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag 5580 ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt atcgccggca 5640 tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct 5700 tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg caggccatgc 5760 tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc gcggctctta 5820 ccagcctaac ttcgatcact ggaccgctga tcgtcacggc gatttatgcc gcctcggcga 5880 gcacatggaa cgggttggca tggattgtag gcgccgccct ataccttgtc tgcctccccg 5940 cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg aatggaagcc ggcggcacct 6000 cgctaacgga ttcaccactc caagaattgg agccaatcaa ttcttgcgga gaactgtgaa 6060 tgcgcaaacc aacccttggc agaacatatc catcgcgtcc gccatctcca gcagccgcac 6120 gcggcgcatc tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc 6180 gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg aatcaccgat 6240 acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag caacaacatg 6300 aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag cgccctgcac 6360 cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc 6420 tgtattaacg aagcgctggc attgaccctg agtgattttt ctctggtccc gccgcatcca 6480 taccgccagt tgtttaccct cacaacgttc cagtaaccgg gcatgttcat catcagtaac 6540 ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt acccccatga acagaaatcc 6600 cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt 6660 tatcagaagc cagacattaa cgcttctgga gaaactcaac gagctggacg cggatgaaca 6720 ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca gctgcctcgc 6780 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc 6840 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg 6900 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt 6960 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg 7020 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac 7080 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 7140 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 7200 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 7260 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 7320 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 7380 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 7440 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 7500 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 7560 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 7620 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 7680 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 7740 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 7800 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 7860 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 7920 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 7980 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 8040 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 8100 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 8160 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 8220 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 8280 gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg 8340 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 8400 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 8460 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 8520 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 8580 atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc 8640 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 8700 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 8760 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 8820 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 8880 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 8940 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa 9000 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt 9060 cgaataaata cctgtgacgg aagatcactt cgcagaataa ataaatcctg gtgtccctgt 9120 tgataccggg aagccctggg ccaacttttg gcgaaaatga gacgttgatc ggcacgtaag 9180 aggttccaac tttcaccata atgaaataag atcactaccg ggcgtatttt ttgagttatc 9240 gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt 9300 tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg 9360 tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa 9420 taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc 9480 ggaa 9484 //