ID pAIL2 standard; circular DNA; UNA; 4504 BP. DE LMBP 3076. CC Accession number: LMBP 3076 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 30-MAY-2012 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT terminator 24..105 FT /vntifkey="43" FT /label=fd\terminator FT terminator 134..215 FT /vntifkey="43" FT /label=fd\terminator FT rep_origin 313..768 FT /vntifkey="33" FT /label=f1\ori FT rep_origin complement(772..1145) FT /vntifkey="33" FT /label=pMB1\ori FT CDS complement(1686..2546) FT /vntifkey="4" FT /label=ampicillin\resistance FT CDS complement(2861..3520) FT /vntifkey="4" FT /label=chloramphenicol\resistance FT promoter 3785..3821 FT /vntifkey="30" FT /label=tac\promoter FT RBS 3822..3857 FT /vntifkey="32" FT /label=5'\UTR\of\lacZ,\incl.\RBS FT RBS 3865..3881 FT /vntifkey="32" FT /label=5'\UTR\of\ompA,\incl.\RBS FT CDS 3882..3941 FT /vntifkey="4" FT /label=ompA\signal\sequence FT /note="E. coli ompA signal sequence, lacking 3' Ala codon" FT CDS 3945..4394 FT /vntifkey="4" FT /label=mIL2\mature\sequence FT /note="mature mouse IL2 cDNA, incl. extra 5' Ser codon" FT misc_feature 4395..4497 FT /vntifkey="21" FT /label=3'\UTR\of\mIL2 SQ Sequence 4504 BP; 1198 A; 1088 C; 1018 G; 1200 t; aattcacctc gaaagcaagc tgataaaccg atacaattaa aggctccttt tggagccttt 60 ttttttggag attttcaacg tgaaaaaatt attattcgca attccaagct aattcacctc 120 gaaagcaagc tgataaaccg atacaattaa aggctccttt tggagccttt ttttttggag 180 attttcaacg tgaaaaaatt attattcgca attccaagct ctgcctcgcg cgtttcggtg 240 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag 300 cggatgcaga tcacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc 360 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt 420 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag 480 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt 540 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt 600 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt 660 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt 720 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttga tctgcgctcg 780 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 840 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 900 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 960 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 1020 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 1080 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 1140 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 1200 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 1260 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 1320 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt 1380 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 1440 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 1500 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 1560 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 1620 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 1680 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 1740 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 1800 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 1860 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 1920 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 1980 cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct 2040 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 2100 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 2160 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 2220 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 2280 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa 2340 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 2400 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 2460 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 2520 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcagacag 2580 ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga 2640 cacaacgtgg ctttgttgaa taaatcgaac ttttgctgag ttgactcccc gcgcgcgatg 2700 ggtcgaattt gctttcgaaa aaaaagcccg ctcattaggc gggctaaaaa aaagcccgct 2760 cattaggcgg gctcgaattt ctgccattca tccgcttatt atcacttatt caggcgtagc 2820 aaccaggcgt ttaagggcac caataactgc cttaaaaaaa ttacgccccg ccctgccact 2880 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacagacgg 2940 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc 3000 ccatagtgaa aacgggggcg aagaagttgt ccatattcgc cacgtttaaa tcaaaactgg 3060 tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga 3120 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc 3180 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa 3240 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac 3300 gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact 3360 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt 3420 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg 3480 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg 3540 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt 3600 tggaacctct tacgtgccga tcaacgtctc attttcgcca aaagttggcc cagggcttcc 3660 cggtatcaac agggacacca ggatttattt attctgcgaa gtgatcttcc gtcacaggta 3720 tttattcgaa gacgaaaggg catcgcgcgc ggggaattcg agctcgagct tactccccat 3780 ccccctgttg acaattaatc atcggctcgt ataatgtgtg gaattgtgag cggataacaa 3840 tttcacacag gaaacaggat cctagataac gagggcaaaa aatgaaaaag acagctatcg 3900 cgattgcagt ggcactggct ggtttcgcta ccgtagcgca gtcagcaccc acttcaagct 3960 ccacttcaag ctctacagcg gaagcacagc agcagcagca gcagcagcag cagcagcagc 4020 agcacctgga gcagctgttg atggacctac aggagctcct gagcaggatg gagaattaca 4080 ggaacctgaa actccccagg atgctcacct tcaaatttta cttgcccaag caggccacag 4140 aattgaaaga tcttcagtgc ctagaagatg aacttggacc tctgcggcat gttctggatt 4200 tgactcaaag caaaagcttt caattggaag atgctgagaa tttcatcagc aatatcagag 4260 taactgttgt aaaactaaag ggctctgaca acacatttga gtgccaattc gatgatgagt 4320 cagcaactgt ggtggacttt ctgaggagat ggatagcctt ctgtcaaagc atcatctcaa 4380 caagccctca ataactatgt acctcctgct tacaacacat aaggctctct atttatttaa 4440 atatttaact ttaatttatt tttggatgta ttgtttacta tcttttgtaa ctactagagg 4500 tcga 4504 //