ID pCDM8hFasR standard; circular DNA; UNA; 5130 BP. DE LMBP 3118. CC Accession number: LMBP 3118 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 29-MAY-2012 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT rep_origin complement(36..111) FT /vntifkey="33" FT /label=SV40\ori FT /note="bidirectional origin of SV40" FT rep_origin complement(262..374) FT /vntifkey="33" FT /label=polyoma\ori FT /note="origin polyoma and enhancer" FT terminator 1063..1159 FT /vntifkey="43" FT /label=SV40\polyA\late FT terminator complement(1063..1159) FT /vntifkey="43" FT /label=SV40\polyA\early FT misc_feature complement(1160..1558) FT /vntifkey="21" FT /label=3'\UTR\of\SV40\small\t-antigen FT /note="3' UTR of the SV40 small t-antigen; with deletion" FT intron complement(1559..1625) FT /vntifkey="15" FT /label=intron\of\SV40\small\t-antigen FT CDS complement(1626..1700) FT /vntifkey="4" FT /label=part\of\SV40\small\t-antigen FT CDS complement(1703..2710) FT /vntifkey="4" FT /label=hFAS FT misc_feature complement(2711..2907) FT /vntifkey="21" FT /label=5'\UTR\of\hFAS FT promoter complement(2945..2961) FT /vntifkey="30" FT /label=T7\promoter FT /note="phage T7 gene 10 promoter" FT promoter complement(3006..3589) FT /vntifkey="29" FT /label=hCMV-IE\promoter\and\enhancer FT tRNA complement(3744..3946) FT /vntifkey="59" FT /label=supF FT /note="suppressor tRNA gene, incl. own promoter" FT rep_origin 4070..4528 FT /vntifkey="33" FT /label=M13\ori FT /note="phage M13 origin" FT rep_origin complement(4547..4779) FT /vntifkey="33" FT /label=pMB1\ori SQ Sequence 5130 BP; 1274 A; 1221 C; 1198 G; 1434 t; 3 other; gaattagctt tttgcaaaag cctaggcctc caaaaaagcc tcctcactac ttctggaata 60 gctcagaggc cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg 120 gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagta agacccgcac 180 ccgttcccaa actgcttatt aaagggggta tggaggtgct ggaccttgtg acagggccag 240 acagtgtgac agaaatagaa gcagtcctgt tttgacaagt tgcctctgga agcctctaca 300 atgcctctct tctttttctc cagagtaagc gggcccaggg gtccccggcc tctgcttaat 360 actaaaaaaa acagctgttg tcatagtaat gattgggtgg aaacattcta ggcctgggtg 420 gagaggcttt tgcttcctct tgcaaaacca cacgccctct ggagggcgtt gcctagcaac 480 taattaaaag aggatgtcgc acggccagct gcgtcagtta gtccacttcc tccttaactg 540 acttgacatt ttctatttta agagtcggga ggaaaattac tgtgttggag gccctcgccc 600 atcttctgaa gctgatcaag taagtgaatt ttcaaaatgg gagccgcact gactattcta 660 gtagacctca tcgagggatt agctgaagtg tctaccctta cgggactctc ggcagaagct 720 attttatctg gagaagccct cgctgccctt gatcgcgaaa ttacagctct cacttggtag 780 ggtgtaatga gttcggagac agccctagca actatgggta tttcagagga ggtgtatggg 840 tttgtcagta ctgtgcctgt gtttgtaagt cgaacagcgg gggctatatg gctgatgcag 900 acagttcaag gtgcctctac tatttcccta ggaatacagc ggtacctaca caacgaagag 960 gtccctactg taaatagaaa tatggcgttg ataccatggc gggatccaga catgataaga 1020 tacattgatg agtttggaca aaccacaact agaatgcagt gataaaaatg ctttatttgt 1080 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac 1140 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa 1200 agcaagtaaa acctctacaa atgtggtatg gctgattatg atctctagtc aaggcactat 1260 acatcaaata tttttccata attttcttgt atagcagtgc agctttttcc tttgtggtgt 1320 aaatagcaaa gcaagcaaga gttctattac taaacacagc atgactcaaa aaacttacca 1380 attctgaagg aaagtccttg gggtcttcta cctttctctt cttttttgga ggagtagaat 1440 gttgagagtc agcagtagcc tcatcatcac tagatggcat ttcttctgag caaaacaggt 1500 tttcctcatt aaaggcattc caccactgct cccattcata agttccatag gttggaatct 1560 aaaataccac aacaattaga atcagtagtt taacacatta tacacttaaa aattttatat 1620 ttaccttaga gctttaaatc tctgtaggta gtttgtccaa ttatgtcaca ccacagaagt 1680 aaggttcctt cacaaagatc ctctagacca agctttggat ttcatttctg aagtttgaat 1740 tttctgagtc actagtaatg tccttgagga tgatagtctg aattttctct gcaagagtac 1800 aaagattggc ttttttgaga tctttaatca atgtgtcata cgcttctttc tttccatgaa 1860 gttgatgcca attacgaagc agttgaactt tctgttctgc tgtgtcttgg acattgtcat 1920 tcttgatctc atctattttg gcttcattga caccattctt tcgaacaaag cctttaactt 1980 gacttagtgt catgactcca gcaatagtgg tgatatattt actcaagtca acatcagata 2040 aatttattgc cactgtttca ggatttaagg ttggagattc atgagaacct tggttttcct 2100 ttctgtgctt tctgcatgtt ttctgtactt cctttctctt cacccaaaca attagtggaa 2160 ttggcaaaag aagaagacaa agccacccca agttagatct ggatccttcc tctttgcact 2220 tggtgttgct ggtgagtgtg cattccttga tgattccatg ttcacatttg gtgcaagggt 2280 cacagtgttc acatacagta gagttacaaa aaaagtttgg tttacatctg cacttggtat 2340 tctgggtccg ggtgcagttt atttccactt ctaagccatg tccttcatca cacaatctac 2400 atcttctgca tttggaagaa aaatgggctt tgtctgtgta ctccttccct tcttggcagg 2460 gcacgcagtc tggttcatcc ccattgactg tgcagtccct agctttcctt tcacctggag 2520 gacagggctt atggcagaat tggccatcat gatgcaggcc ttccaagttc tgagtctcaa 2580 ctgtagtaac agtcttcctc aattccaatc ccttggagtt gatgtcagtc acttgggcat 2640 taacactttt ggacgataat ctagcaacag acgtaagaac cagaggtagg agggtccaga 2700 tgcccagcat ggttgttgag caatcctccg aagtgaaaga gcttccccaa ctccgtactg 2760 agcgggtcca ccaacccgcg ggagaagagg cagctccgcc tgggcagcca gggctggcct 2820 cagggtgtgt tccgtgccag tgcccgcccc tgaggctcca gccaagtcac tcgtaaaccg 2880 cttccctcac tccccagaag cgtcnnnctc gaggtcgagg gatctctaga agcttgggtc 2940 tccctatagt gagtcgtatt aatttcgata agccagtaag cagtgggttc tctagttagc 3000 cagagagctc tgcttatata gacctcccac cgtacacgcc taccgcccat ttgcgtcaat 3060 ggggcggagt tgttacgaca ttttggaaag tcccgttgat tttggtgcca aaacaaactc 3120 ccattgacgt caatggggtg gagacttgga aatccccgtg agtcaaaccg ctatccacgc 3180 ccattgatgt actgccaaaa ccgcatcacc atggtaatag cgatgactaa tacgtagatg 3240 tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca ggcgggccat 3300 ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg atgtactgcc 3360 aagtgggcag tttaccgtaa atagtccacc cattgacgtc aatggaaagt ccctattggc 3420 gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag 3480 ccaggcgggc catttaccgt aagttatgta acgcggaact ccatatatgg gctatgaact 3540 aatgaccccg taattgatta ctattaataa ctagtcaata atcaatgtca acgcgtatat 3600 ctggcccgta catcgcgaag cagcgcaaaa cgcctaaccc taagcagatt cttcatgcaa 3660 ttgtcggtca agccttgcct tgttgtagct taaattttac tcgcgcacta ctcagcgacc 3720 tccaacacac aagcagggag cagattcttt cggacttttg aaagtgatgg tggtggggga 3780 aggattcgaa ccttcgaagt cgatgacggc agatttagag tctgctccct ttggccgctc 3840 gggaacccca ccacgggtaa tgcttttact ggcctgctcc cttatcggga agcggggcgc 3900 atcatatcaa atgacgcgcc gctgtaaagt gttacgttga gaaagaccgc ggtaatccag 3960 ctggcgtacc gttcctgtct aaaatccctt taatcggcct cctgtttagc tcccgctctg 4020 attccaacga ggaaagcacg ttatacggtc tcgtcaaagc aaccatagta cgcgccctgt 4080 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc 4140 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc 4200 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg 4260 cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcg gccatcgccc 4320 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg 4380 ttccaaactg gaacaacact caaccctatc tcgggctatt cttttgattt ataagggatt 4440 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat 4500 tttaacaaaa tattaacgtt tacaatttct agctagaagc tagcttgcgt tgctggcgtt 4560 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 4620 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 4680 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 4740 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 4800 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 4860 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 4920 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 4980 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 5040 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 5100 tttttttgtt tgcaagcagc agattacgcc 5130 //