LOCUS       pBLCAT5HIL6234          4464 bp    DNA     circular     21-AUG-2013
DEFINITION  LMBP 3231.
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|670931450|
COMMENT     VNTNAME|pBLCAT5HIL6234|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pBLCAT5HIL6234" 0 4464 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "NheI" "XbaI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #3=(CRSite (CStringList) "NheI" "gctagc" 2 0 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "XbaI" "tctaga" 2 0 0 0 "")) 
COMMENT       (CObList 
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COMMENT            1 1 1 1 "1260..1919") 
COMMENT        #6=(CFSignal (CObList) "(t)" 4 0 0 1051 1125 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "2009..2083") 
COMMENT        #7=(CFSignal (CObList) "INT" 15 0 0 1126 1192 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "2084..2150") 
COMMENT        #8=(CFSignal (CObList) "3UTR" 21 0 0 1193 1744 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "2151..2702") 
COMMENT        #9=(CFSignal (CObList) "polyA" 43 0 0 1745 1841 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "2703..2799") 
COMMENT        #10=(CFSignal (CObList) "polyA" 43 0 1 1745 1841 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2703..2799)") 
COMMENT        #11=(CFSignal (CObList) "lac" 30 0 1 1980 2063 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2938..3021)") 
COMMENT        #12=(CFSignal (CObList) "ORI" 33 0 1 2155 2557 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3113..3515)") 
COMMENT        #13=(CFSignal (CObList) "AMP" 4 0 1 3098 3958 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(4056..4916)") 
COMMENT        #14=(CFSignal (CObList) "polyA" 43 0 0 4090 4164 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5048..5122") 
COMMENT        #15=(CFSignal (CObList) "polyA" 43 0 1 4090 4164 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(5048..5122)") 
COMMENT        #16=(CFSignal (CObList) "polyA" 43 0 0 4305 4401 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5263..5359") 
COMMENT        #17=(CFSignal (CObList) "polyA" 43 0 1 4305 4401 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(5263..5359)") 
COMMENT        #18=(CFSignal (CObList) "hIL6 promoter" 29 0 0 26 250 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) 
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COMMENT                          "DNA 'pBLCAT5HIL6234'" 1) 
COMMENT                     #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 08/21/13 09:50" 1) 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 08/21/13 09:50" 1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 4464 bp" 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 19-MAR-1980" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                   "Annotations") 
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COMMENT                                        "Start: 302   End: 961 " 1) 
COMMENT                                   #38=(CLinePar 
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COMMENT                                        "chloramphenicol acetyltransferase coding sequence" 
COMMENT                                        1) 
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COMMENT                                        "Start: 1051  End: 1125" 1) 
COMMENT                                   #43=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "part of the SV40 small t-antigen" 1) 
COMMENT                                   #44=(CLinePar 
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COMMENT                                   #45=(CLinePar 
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COMMENT                                        "   2009..2083" 1))) "(t)") 
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COMMENT                                        "Start: 3098  End: 3958 (Complementary)" 
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COMMENT                                   #48=(CLinePar 
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COMMENT                                        "antibiotic resistance gene; coding sequence" 
COMMENT                                        1) 
COMMENT                                   #49=(CLinePar 
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COMMENT                          "CDS (3 total)") 
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COMMENT                                        "Start: 1126  End: 1192" 1) 
COMMENT                                   #54=(CLinePar 
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COMMENT                                        "intron of the SV40 small t-antigen" 1) 
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COMMENT                                        "3' UTR of the SV40 small t-antigen" 1) 
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COMMENT                          "Misc. Feature (1 total)") 
COMMENT                     #63=(CFolderPar 
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COMMENT                                        "Start: 26    End: 250 " 1))) 
COMMENT                                 "hIL6 promoter"))) 
COMMENT                          "Promoter Eukaryotic (1 total)") 
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COMMENT                                        "E. coli lac operon promoter" 1) 
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COMMENT                                        "Start: 2155  End: 2557 (Complementary)" 
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COMMENT                                   #75=(CLinePar 
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COMMENT                          "Replication Origin (1 total)") 
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COMMENT                                    #106=(CLinePar 
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COMMENT                                  "polyA"))) "Terminator (6 total)"))) 
COMMENT                   "Feature Map") 
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FEATURES             Location/Qualifiers
     CDS             302..961
                     /vntifkey="4"
                     /label=cat
                     /note="chloramphenicol acetyltransferase coding sequence"
     CDS             1051..1125
                     /vntifkey="4"
                     /label=(t)
                     /note="part of the SV40 small t-antigen"
     intron          1126..1192
                     /vntifkey="15"
                     /label=INT
                     /note="intron of the SV40 small t-antigen"
     misc_feature    1193..1744
                     /vntifkey="21"
                     /label=3UTR
                     /note="3' UTR of the SV40 small t-antigen"
     terminator      1745..1841
                     /vntifkey="43"
                     /label=polyA
                     /note="polyA signal of the SV40 early region"
     terminator      complement(1745..1841)
                     /vntifkey="43"
                     /label=polyA
                     /note="polyA signal of the SV40 late region"
     promoter        complement(1980..2063)
                     /vntifkey="30"
                     /label=lac
                     /note="E. coli lac operon promoter"
     rep_origin      complement(2155..2557)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     CDS             complement(3098..3958)
                     /vntifkey="4"
                     /label=AMP
                     /note="antibiotic resistance gene; coding sequence"
     terminator      4090..4164
                     /vntifkey="43"
                     /label=polyA
                     /note="polyA signal of the SV40 early region"
     terminator      complement(4090..4164)
                     /vntifkey="43"
                     /label=polyA
                     /note="polyA signal of the SV40 late region"
     terminator      4305..4401
                     /vntifkey="43"
                     /label=polyA
                     /note="polyA signal of the SV40 early region"
     terminator      complement(4305..4401)
                     /vntifkey="43"
                     /label=polyA
                     /note="polyA signal of the SV40 late region"
     promoter        26..250
                     /vntifkey="29"
                     /label=hIL6\promoter
BASE COUNT     1222 a      1018 c       963 g      1261 t 
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctagc ctcaatgacg acctaagctg cacttttccc 
       61 cctagttgtg tcttgcgatg ctaaaggacg tcacattgca caatcttaat aaggtttcca 
      121 atcagcccca cccgctctgg ccccaccctc accctccaac aaagatttat caaatgtggg 
      181 attttcccat gagtctcaat attagagtct caacccccaa taaatatagg actggagatg 
      241 tctgaggctc attctgccct cgaggagctt ggcgagattt tcaggagcta aggaagctaa 
      301 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga 
      361 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga 
      421 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat 
      481 tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg 
      541 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga 
      601 aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata 
      661 ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga 
      721 gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt 
      781 ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg 
      841 cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca 
      901 tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta 
      961 atttttttaa ggcagttatt ggtgccctta aacgcctggt gctacgcctg aataagtgat 
     1021 aataagcgga tgaatggcag aaattcgccg gatctttgtg aaggaacctt acttctgtgg 
     1081 tgtgacataa ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt 
     1141 tttaagtgta taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac 
     1201 ctatggaact gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg 
     1261 ctcagaagaa atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc 
     1321 tccaaaaaag aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt 
     1381 tttgagtcat gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa 
     1441 ggaaaaagct gcactgctat acaagaaaat tatggaaaaa tattctgtaa cctttataag 
     1501 taggcataac agttataatc ataacatact gttttttctt actccacaca ggcatagagt 
     1561 gtctgctatt aataactatg ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg 
     1621 ggttaataag gaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca 
     1681 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac 
     1741 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat 
     1801 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg 
     1861 gtttgtccaa actcatcaat gtatcttatc atgtctggat cgatccccgg gtaccgagct 
     1921 cgaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 
     1981 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct 
     2041 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 
     2101 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 
     2161 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 
     2221 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 
     2281 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 
     2341 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 
     2401 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 
     2461 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 
     2521 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 
     2581 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 
     2641 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 
     2701 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 
     2761 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 
     2821 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 
     2881 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 
     2941 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 
     3001 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 
     3061 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 
     3121 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 
     3181 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 
     3241 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 
     3301 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 
     3361 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 
     3421 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 
     3481 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 
     3541 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 
     3601 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 
     3661 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 
     3721 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 
     3781 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 
     3841 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 
     3901 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 
     3961 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 
     4021 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 
     4081 tgccacctga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 
     4141 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 
     4201 aatgtatctt atcatgtctg gatcataatc agccatacca catttgtaga ggttttactt 
     4261 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt 
     4321 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 
     4381 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 
     4441 gtatcttatc atgtctggat ctcg 
//
