LOCUS pBR325 5994 bp DNA circular 23-OCT-2007 DEFINITION LMBP 324. SOURCE ORGANISM COMMENT Accession number: LMBP 324 (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium). Last modified: 23-OCT-2007 COMMENT VNTNAME|pBR325| COMMENT VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection| COMMENT VNTOAUTHORTEL|0032-9-33-13-843| COMMENT VNTOAUTHOREML|bccm.genecorner@UGent.be| COMMENT VNTOAUTHORWWW|www.genecorner.ugent.be| COMMENT VNTOAUTHORAD1|Ghent University| COMMENT VNTOAUTHORAD2|Technologiepark 927| COMMENT VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| COMMENT VNTREPLTYPE|Plasmid COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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Location/Qualifiers CDS 1301..2491 /vntifkey="4" /label=tetracycline /note="antibiotic resistance gene; coding sequence" rep_origin complement(3565..3967) /vntifkey="33" /label=pMB1\ori /note="origin plasmid" CDS complement(4508..5368) /vntifkey="4" /label=ampicillin /note="antibiotic resistance gene; coding sequence" CDS 5779..444 /vntifkey="4" /label=chloramphenicol /note="antibiotic resistance gene; coding sequence" BASE COUNT 1394 a 1600 c 1565 g 1435 t ORIGIN 1 ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac 61 accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat 121 ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc 181 tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt 241 ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc 301 atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat 361 catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc 421 gatgagtggc agggcggggc gtaatttttt taaggcagtt attggtgccc ttaaacgcct 481 ggtgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg aaagcaaatt 541 cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct attgctggtt 601 taccggttta ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca 661 gtttgctcag gctctccccg tggaggtaat aattgacgat atgatcattt attctgcctc 721 ccagagcctg ataaaaacgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc 781 gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg 841 gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg 901 acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc 961 tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg 1021 atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac 1081 gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg 1141 ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg 1201 aataccgcaa gcgacaggcc atgtttgaca gcttatcatc gataagcttt aatgcggtag 1261 tttatcacag ttaaattgct aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct 1321 catcgtcatc ctcggcaccg tcaccctgga tgctgtaggc ataggcttgg ttatgccggt 1381 actgccgggc ctcttgcggg atatcgtcca ttccgacagc atcgccagtc actatggcgt 1441 gctgctagcg ctatatgcgt tgatgcaatt tctatgcgca cccgttctcg gagcactgtc 1501 cgaccgcttt ggccgccgcc cagtcctgct cgcttcgcta cttggagcca ctatcgacta 1561 cgcgatcatg gcgaccacac ccgtcctgtg gatcctctac gccggacgca tcgtggccgg 1621 catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga 1681 agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg 1741 ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc 1801 ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg 1861 agagcgtcga ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg 1921 gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca 1981 ggtgccggca gcgctctggg tcattttcgg cgaggaccgc tttcgctgga gcgcgacgat 2041 gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac 2101 tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt atcgccggca tggcggccga 2161 cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct tccccattat 2221 gattcttctc gcttccggcg gcatcgggat gcccgcgttg caggccatgc tgtccaggca 2281 ggtagatgac gaccatcagg gacagcttca aggatcgctc gcggctctta ccagcctaac 2341 ttcgatcact ggaccgctga tcgtcacggc gatttatgcc gcctcggcga gcacatggaa 2401 cgggttggca tggattgtag gcgccgccct ataccttgtc tgcctccccg cgttgcgtcg 2461 cggtgcatgg agccgggcca cctcgacctg aatggaagcc ggcggcacct cgctaacgga 2521 ttcaccactc caagaattgg agccaatcaa ttcttgcgga gaactgtgaa tgcgcaaacc 2581 aacccttggc agaacatatc catcgcgtcc gccatctcca gcagccgcac gcggcgcatc 2641 tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc gttgaggacc 2701 cggctaggct ggcggggttg ccttactggt tagcagaatg aatcaccgat acgcgagcga 2761 acgtgaagcg actgctgctg caaaacgtct gcgacctgag caacaacatg aatggtcttc 2821 ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag cgccctgcac cattatgttc 2881 cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc tgtattaacg 2941 aagcgctggc attgaccctg agtgattttt ctctggtccc gccgcatcca taccgccagt 3001 tgtttaccct cacaacgttc cagtaaccgg gcatgttcat catcagtaac ccgtatcgtg 3061 agcatcctct ctcgtttcat cggtatcatt acccccatga acagaaatcc cccttacacg 3121 gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt tatcagaagc 3181 cagacattaa cgcttctgga gaaactcaac gagctggacg cggatgaaca ggcagacatc 3241 tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca gctgcctcgc gcgtttcggt 3301 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa 3361 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg 3421 ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg 3481 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg 3541 taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct 3601 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 3661 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 3721 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 3781 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 3841 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 3901 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 3961 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 4021 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 4081 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 4141 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg 4201 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 4261 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 4321 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 4381 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 4441 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 4501 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 4561 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 4621 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 4681 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 4741 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 4801 tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg 4861 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 4921 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 4981 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 5041 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 5101 cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa 5161 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 5221 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 5281 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 5341 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 5401 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 5461 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta 5521 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt cgaataaata 5581 cctgtgacgg aagatcactt cgcagaataa ataaatcctg gtgtccctgt tgataccggg 5641 aagccctggg ccaacttttg gcgaaaatga gacgttgatc ggcacgtaag aggttccaac 5701 tttcaccata atgaaataag atcactaccg ggcgtatttt ttgagttatc gagattttca 5761 ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc 5821 caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac 5881 cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag 5941 ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc ggaa //