LOCUS       pBLhIL6LUC              6470 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 3287.
SOURCE      
  ORGANISM  
COMMENT     VNTNAME|pBLhIL6LUC|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pBLhIL6LUC" 0 6470 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
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COMMENT        #5=(CFSignal (CObList) "LUC" 4 0 0 1250 2902 0 (CStringList) 
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COMMENT        #6=(CFSignal (CObList) "(t)" 4 0 0 3062 3136 0 (CStringList) 
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COMMENT        #10=(CFSignal (CObList) "polyA" 43 0 1 3756 3852 0 (CStringList) 
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COMMENT        #11=(CFSignal (CObList) "lac" 30 0 1 3986 4069 0 (CStringList) 
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COMMENT        #12=(CFSignal (CObList) "ORI" 33 0 1 4161 4563 0 (CStringList) 
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COMMENT        #13=(CFSignal (CObList) "AMP" 4 0 1 5104 5964 0 (CStringList) 
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COMMENT                          "DNA 'pBLhIL6LUC'" 1) 
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COMMENT                          "Foreign object. Author: UNKNOWN. Original author: BCCM/GeneCorner Plasmid Collection" 
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COMMENT                                        "antibiotic resistance gene; coding sequence" 
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FEATURES             Location/Qualifiers
     misc_feature    32..672
                     /vntifkey="21"
                     /label=5FR
                     /note="5' flanking region of the human interleukin 6 gene"
     promoter        673..1208
                     /vntifkey="30"
                     /label=hIL6
                     /note="human interleukin 6 promoter"
     CDS             1250..2902
                     /vntifkey="4"
                     /label=LUC
                     /note="firefly luciferase coding sequence"
     CDS             3062..3136
                     /vntifkey="4"
                     /label=(t)
                     /note="part of the SV40 small t-antigen"
     intron          3137..3203
                     /vntifkey="15"
                     /label=INT
                     /note="intron of the SV40 small t-antigen"
     misc_feature    3204..3755
                     /vntifkey="21"
                     /label=3UTR
                     /note="3' UTR of the SV40 small t-antigen"
     terminator      3756..3852
                     /vntifkey="43"
                     /label=polyA
                     /note="polyA signal of the SV40 early region"
     terminator      complement(3756..3852)
                     /vntifkey="43"
                     /label=polyA
                     /note="polyA signal of the SV40 late region"
     promoter        complement(3986..4069)
                     /vntifkey="30"
                     /label=lac
                     /note="E. coli lac operon promoter"
     rep_origin      complement(4161..4563)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     CDS             complement(5104..5964)
                     /vntifkey="4"
                     /label=AMP
                     /note="antibiotic resistance gene; coding sequence"
     terminator      6096..6170
                     /vntifkey="43"
                     /label=polyA
                     /note="polyA signal of the SV40 early region"
     terminator      complement(6096..6170)
                     /vntifkey="43"
                     /label=polyA
                     /note="polyA signal of the SV40 late region"
     terminator      6311..6407
                     /vntifkey="43"
                     /label=polyA
                     /note="polyA signal of the SV40 early region"
     terminator      complement(6311..6407)
                     /vntifkey="43"
                     /label=polyA
                     /note="polyA signal of the SV40 late region"
BASE COUNT     1830 a      1439 c      1450 g      1751 t 
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatcctcct gcaagagaca ccatcctgag 
       61 ggaagagggc ttctgaacca gcttgaccca ataagaaatt cttgggtgcc gacgcggaag 
      121 cagattcaga gcctagagcc gtgcctgcgt ccgtagtttc cttctagctt cttttgattt 
      181 caaatcaaga cttacaggga gagggagcga taaacacaaa ctctgcaaga tgccacaagg 
      241 tcctcctttg acatccccaa caaagaggtg agtagtattc tccccctttc tgccctgaac 
      301 caagtgggct tcagtaattt cagggctcca ggagacctgg ggcccatgca ggtgccccag 
      361 tgaaacagtg gtgaagagac tcagtggcaa tggggagagc actggcagca caaggcaaac 
      421 ctctggcaca gagagcaaag tcctcactgg gaggattccc aaggggtcac ttgggagagg 
      481 gcagggcagc agccaacctc ctctaagtgg gctgaagcag gtgaagaaag tggcagaagc 
      541 cacgcggtgg caaaaaggag tcacacactc cacctggaga cgccttgaag taactgcacg 
      601 aaatttgagg atggccaggc agttctacaa cagccgctca cagggagagc cagaacacag 
      661 aagaactcag atgactggta gtattacctt cttcataatc ccaggcttgg ggggctgcga 
      721 tggagtcaga ggaaactcag ttcagaacat ctttggtttt tacaaataca aattaactgg 
      781 aacgctaaat tctagcctgt taatctggtc actgaaaaaa aatttttttt ttttcaaaaa 
      841 acatagcttt agcttatttt ttttctcttt gtaaaacttc gtgcatgact tcagctttac 
      901 tctttgtcaa gacatgccaa agtgctgagt cactaataaa agaaaaaaag aaagtaaagg 
      961 aagagtggtt ctgcttctta gcgctagcct caatgacgac ctaagctgca cttttccccc 
     1021 tagttgtgtc ttgcgatgct aaaggacgtc acattgcaca atcttaataa ggtttccaat 
     1081 cagccccacc cgctctggcc ccaccctcac cctccaacaa agatttatca aatgtgggat 
     1141 tttcccatga gtctcaatat tagagtctca acccccaata aatataggac tggagatgtc 
     1201 tgaggctcat tctgccctcg aagcttggca ttccggtact gttggtaaaa tggaagacgc 
     1261 caaaaacata aagaaaggcc cggcgccatt ctatcctcta gaggatggaa ccgctggaga 
     1321 gcaactgcat aaggctatga agagatacgc cctggttcct ggaacaattg cttttacaga 
     1381 tgcacatatc gaggtgaaca tcacgtacgc ggaatacttc gaaatgtccg ttcggttggc 
     1441 agaagctatg aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa 
     1501 ctctcttcaa ttctttatgc cggtgttggg cgcgttattt atcggagttg cagttgcgcc 
     1561 cgcgaacgac atttataatg aacgtgaatt gctcaacagt atgaacattt cgcagcctac 
     1621 cgtagtgttt gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa aaaaattacc 
     1681 aataatccag aaaattatta tcatggattc taaaacggat taccagggat ttcagtcgat 
     1741 gtacacgttc gtcacatctc atctacctcc cggttttaat gaatacgatt ttgtaccaga 
     1801 gtcctttgat cgtgacaaaa caattgcact gataatgaat tcctctggat ctactgggtt 
     1861 acctaagggt gtggcccttc cgcatagaac tgcctgcgtc agattctcgc atgccagaga 
     1921 tcctattttt ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg ttccattcca 
     1981 tcacggtttt ggaatgttta ctacactcgg atatttgata tgtggatttc gagtcgtctt 
     2041 aatgtataga tttgaagaag agctgttttt acgatccctt caggattaca aaattcaaag 
     2101 tgcgttgcta gtaccaaccc tattttcatt cttcgccaaa agcactctga ttgacaaata 
     2161 cgatttatct aatttacacg aaattgcttc tgggggcgca cctctttcga aagaagtcgg 
     2221 ggaagcggtt gcaaaacgct tccatcttcc agggatacga caaggatatg ggctcactga 
     2281 gactacatca gctattctga ttacacccga gggggatgat aaaccgggcg cggtcggtaa 
     2341 agttgttcca ttttttgaag cgaaggttgt ggatctggat accgggaaaa cgctgggcgt 
     2401 taatcagaga ggcgaattat gtgtcagagg acctatgatt atgtccggtt atgtaaacaa 
     2461 tccggaagcg accaacgcct tgattgacaa ggatggatgg ctacattctg gagacatagc 
     2521 ttactgggac gaagacgaac acttcttcat agttgaccgc ttgaagtctt taattaaata 
     2581 caaaggatat caggtggccc ccgctgaatt ggaatcgata ttgttacaac accccaacat 
     2641 cttcgacgcg ggcgtggcag gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt 
     2701 tgttgttttg gagcacggaa agacgatgac ggaaaaagag atcgtggatt acgtcgccag 
     2761 tcaagtaaca accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa 
     2821 aggtcttacc ggaaaactcg acgcaagaaa aatcagagag atcctcataa aggccaagaa 
     2881 gggcggaaag tccaaattgt aaaatgtaac tgtattcagc gatgacgaaa ttcttagcta 
     2941 ttgtaatatt atatgcaaat tgatgaatgg taattttgta attgtgggtc actgtactat 
     3001 tttaacgaat aataaaatca ggtataggta actaaaaagg aattcgagct cggtacccgg 
     3061 ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag 
     3121 agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga 
     3181 ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt 
     3241 ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg 
     3301 aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc 
     3361 ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa 
     3421 ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa 
     3481 ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat cataacatac 
     3541 tgttttttct tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat 
     3601 tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg 
     3661 ccttgactag agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa 
     3721 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac 
     3781 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 
     3841 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 
     3901 catgtctgga tcccgggtac cgagctcgaa ttcgtaatca tggtcatagc tgtttcctgt 
     3961 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 
     4021 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 
     4081 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 
     4141 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 
     4201 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 
     4261 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 
     4321 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 
     4381 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 
     4441 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 
     4501 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 
     4561 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 
     4621 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 
     4681 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 
     4741 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat 
     4801 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 
     4861 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 
     4921 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 
     4981 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 
     5041 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 
     5101 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 
     5161 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 
     5221 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 
     5281 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 
     5341 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 
     5401 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 
     5461 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 
     5521 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 
     5581 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 
     5641 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 
     5701 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 
     5761 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 
     5821 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 
     5881 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 
     5941 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 
     6001 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 
     6061 ggttccgcgc acatttcccc gaaaagtgcc acctgaactt gtttattgca gcttataatg 
     6121 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt 
     6181 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc ataatcagcc 
     6241 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc 
     6301 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt 
     6361 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta 
     6421 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatctcg 
//
