LOCUS       pENTR207-CATMAXyZZZZZ        3607 bp    DNA     circular     21-JUN-2011
DEFINITION  LMBP 337.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 337
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Reference: Hilson et al., Genome Research 14 (2004), 2176-2189
            Last modified: 21-JUN-2011
            
            
            
            
COMMENT     VNTDATE|424787367|
COMMENT     VNTNAME|pENTR207-CATMAXyZZZZZ|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

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COMMENT                                    (LOGPEN 0 0 0) 1 1.16464 6.23244 0.0365226) 
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COMMENT                                   4.77538 1.01066 #170#) 
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COMMENT                                   4.77538 1.01066 #172#) 
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COMMENT                                   12.7838 0 -10 8.31269 1.01066 #174#) 
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FEATURES             Location/Qualifiers
     CDS             2329..2511
                     /vntifkey="4"
                     /label=(kanamycin)
                     /note="3' fragment of the kanamycin resistance CDS"
     terminator      complement(3536..3579)
                     /vntifkey="43"
                     /label=T1\terminator
                     /note="Escherichia coli rrnB operon T1 terminator"
     terminator      complement(3377..3404)
                     /vntifkey="43"
                     /label=T2\terminator
                     /note="Escherichia coli rrnB operon T2 terminator"
     rep_origin      3024..3349
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             587..1213
                     /vntifkey="4"
                     /label=(kanamycin)
                     /note="5' fragment of the kanamycin resistance CDS"
     primer          3587..3606
                     /vntifkey="27"
                     /label=DNR5\primer
     primer          complement(813..832)
                     /vntifkey="27"
                     /label=DNR3\primer
     attenuator      28..127
                     /vntifkey="0"
                     /label=attL1
     attenuator      complement(364..464)
                     /vntifkey="0"
                     /label=attL2
     CDS             complement(1247..1780)
                     /vntifkey="4"
                     /label=gentamycin
     primer          130..146
                     /vntifkey="27"
                     /label=5'\gene\specific\primer
     primer          complement(347..363)
                     /vntifkey="27"
                     /label=3'\gene\specific\primer
     CDS             147..346
                     /vntifkey="4"
                     /label=CATMAXyZZZZZ
                     /note="GST template"
BASE COUNT      811 a       827 c       859 g       876 t       234 others
ORIGIN
        1 gttaacgcta gcatggatct cgggccccaa ataatgattt tattttgact gatagtgacc 
       61 tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa 
      121 gcaggcttcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
      181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
      361 nnngacccag ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt 
      421 tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgcagct 
      481 ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg 
      541 aacaataaaa ctgtctgctt acataaacag taatacaggg ggtgttatga gccatattca 
      601 acgggaaacg tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa 
      661 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc 
      721 cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga 
      781 tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt 
      841 tatccgtact cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt 
      901 ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt 
      961 cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt 
     1021 tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga 
     1081 tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc 
     1141 attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga 
     1201 cgaggggaaa ttaggccggg aagccgatct cggcttgaac gaattgttag gtggcggtac 
     1261 ttgggtcgat atcaaagtgc atcacttctt cccgtatgcc caactttgta tagagagcca 
     1321 ctgcgggatc gtcaccgtaa tctgcttgca cgtagatcac ataagcacca agcgcgttgg 
     1381 cctcatgctt gaggagattg atgagcgcgg tggcaatgcc ctgcctccgg tgctcgccgg 
     1441 agactgcgag atcatagata tagatctcac tacgcggctg ctcaaacctg ggcagaacgt 
     1501 aagccgcgag agcgccaaca accgcttctt ggtcgaaggc agcaagcgcg atgaatgtct 
     1561 tactacggag caagttcccg aggtaatcgg agtccggctg atgttgggag taggtggcta 
     1621 cgtctccgaa ctcacgaccg aaaagatcaa gagcagcccg catggatttg acttggtcag 
     1681 ggccgagcct acatgtgcga atgatgccca tacttgagcc acctaacttt gttttagggc 
     1741 gactgccctg ctgcgtaaca tcgttgctgc tgcgtaacat cgttgctgct ccataacatc 
     1801 aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtaca 
     1861 aaaaaacagt cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg 
     1921 gtcaaggttc tggaccagtt gcgtgagcgc atacgctact tgcattacag tttacgaacc 
     1981 gaacaggctt atgtcaactg ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg 
     2041 gcaaccttgg gcagcagcga agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag 
     2101 gtttcggtct ccacgcatcg tcaggcattg gcggccttgc tgttcttcta cggcaaggtg 
     2161 ctgtgcacgg atctgccctg gcttcaggag atcggaagac ctcggccgtc gcggcgcttg 
     2221 ccggtggtgc tgaccccgga tgaagtggtt cgcatcctcg gttttctgga aggcgagcat 
     2281 cgtttgttcg cccaggactc tagctatagt tctagtggtt ggctactaat aggttgtatt 
     2341 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc 
     2401 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat 
     2461 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg 
     2521 gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca 
     2581 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga 
     2641 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa 
     2701 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga 
     2761 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt 
     2821 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt 
     2881 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat 
     2941 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct 
     3001 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca 
     3061 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag 
     3121 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc 
     3181 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga 
     3241 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca 
     3301 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgct agccaggaag 
     3361 agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct 
     3421 ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa 
     3481 atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa 
     3541 acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcctgg cagttcccta 
     3601 ctctcgc 
//
