ID pBIC standard; circular DNA; ; 6479 BP. DE LMBP 3450. CC Accession number: LMBP 3450 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 13-MAR-2009 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT RBS complement(2600..3060) FT /vntifkey="32" FT /label=5'\UTR\of\FMDV-POLYP\(IRES) FT /note="5' UTR of the C-S8c1 foot and mouth disease virus polyprotein gene (FMDV)" FT CDS complement(3126..3785) FT /vntifkey="4" FT /label=cat FT /note="chloramphenicol acetyltransferase coding sequence" FT terminator complement(64..160) FT /vntifkey="43" FT /label=SV40\polyA\early FT /note="polyA signal of the SV40 early region" FT terminator 64..160 FT /vntifkey="43" FT /label=SV40\polyA\late FT /note="polyA signal of the SV40 late region" FT misc_feature complement(161..779) FT /vntifkey="21" FT /label=3'\UTR\of\SV40\small\t-antigen FT /note="part of the 3' UTR of the SV40 small t-antigen" FT misc_feature complement(867..911) FT /vntifkey="21" FT /label=3'\UTR\of\LUC FT /note="3' UTR of the firefly luciferase gene" FT CDS complement(912..2564) FT /vntifkey="4" FT /label=LUC FT /note="firefly luciferase coding sequence" FT promoter complement(3813..3921) FT /vntifkey="29" FT /label=HSV-TK\promoter FT /note="Herpes simplex thymidine kinase promoter" FT promoter complement(3954..3970) FT /vntifkey="30" FT /label=T7\promoter FT /note="phage T7 gene 10 promoter" FT rep_origin complement(4038..4440) FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT CDS complement(4981..5841) FT /vntifkey="4" FT /label=ampicillin\resistance SQ Sequence 6479 BP; 1700 A; 1553 C; 1494 G; 1732 t; ggggatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag 60 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata 120 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg 180 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat 240 gatctctagt caaggcacta tacatcaaat attccttatt aaccccttta caaattaaaa 300 agctaaaggt acacaatttt tgagcatagt tattaatagc agacactcta tgcctgtgtg 360 gagtaagaaa aaacagtatg ttatgattat aactgttatg cctacttata aaggttacag 420 aatatttttc cataattttc ttgtatagca gtgcagcttt ttcctttgtg gtgtaaatag 480 caaagcaagc aagagttcta ttactaaaca cagcatgact caaaaaactt agcaattctg 540 aaggaaagtc cttggggtct tctacctttc tcttcttttt tggaggagta gaatgttgag 600 agtcagcagt agcctcatca tcactagatg gcatttcttc tgagcaaaac aggttttcct 660 cattaaaggc attccaccac tgctcccatt catcagttcc ataggttgga atctaaaata 720 cacaaacaat tagaatcagt agtttaacac attatacact taaaaatttt atatttacct 780 tagagcttta aatctctgta ggtagtttgt ccaattatgt cacaccacag aagtaaggtt 840 ccttcacaaa gagatcctct agagtcatta caatagctaa gaatttcgtc atcgctgaat 900 acagttacat tttacaattt ggactttccg cccttcttgg cctttatgag gatctctctg 960 atttttcttg cgtcgagttt tccggtaaga cctttcggta cttcgtccac aaacacaact 1020 cctccgcgca actttttcgc ggttgttact tgactggcga cgtaatccac gatctctttt 1080 tccgtcatcg tctttccgtg ctccaaaaca acaacggcgg cgggaagttc accggcgtca 1140 tcgtcgggaa gacctgccac gcccgcgtcg aagatgttgg ggtgttgtaa caatatcgat 1200 tccaattcag cgggggccac ctgatatcct ttgtatttaa ttaaagactt caagcggtca 1260 actatgaaga agtgttcgtc ttcgtcccag taagctatgt ctccagaatg tagccatcca 1320 tccttgtcaa tcaaggcgtt ggtcgcttcc ggattgttta cataaccgga cataatcata 1380 ggtcctctga cacataattc gcctctctga ttaacgccca gcgttttccc ggtatccaga 1440 tccacaacct tcgcttcaaa aaatggaaca actttaccga ccgcgcccgg tttatcatcc 1500 ccctcgggtg taatcagaat agctgatgta gtctcagtga gcccatatcc ttgtcgtatc 1560 cctggaagat ggaagcgttt tgcaaccgct tccccgactt ctttcgaaag aggtgcgccc 1620 ccagaagcaa tttcgtgtaa attagataaa tcgtatttgt caatcagagt gcttttggcg 1680 aagaatgaaa atagggttgg tactagcaac gcactttgaa ttttgtaatc ctgaagggat 1740 cgtaaaaaca gctcttcttc aaatctatac attaagacga ctcgaaatcc acatatcaaa 1800 tatccgagtg tagtaaacat tccaaaaccg tgatggaatg gaacaacact taaaatcgca 1860 gtatccggaa tgatttgatt gccaaaaata ggatctctgg catgcgagaa tctgacgcag 1920 gcagttctat gcggaagggc cacaccctta ggtaacccag tagatccaga ggaattcatt 1980 atcagtgcaa ttgttttgtc acgatcaaag gactctggta caaaatcgta ttcattaaaa 2040 ccgggaggta gatgagatgt gacgaacgtg tacatcgact gaaatccctg gtaatccgtt 2100 ttagaatcca tgataataat tttctggatt attggtaatt ttttttgcac gttcaaaatt 2160 ttttgcaacc cctttttgga aacaaacact acggtaggct gcgaaatgtt catactgttg 2220 agcaattcac gttcattata aatgtcgttc gcgggcgcaa ctgcaactcc gataaataac 2280 gcgcccaaca ccggcataaa gaattgaaga gagttttcac tgcatacgac gattctgtga 2340 tttgtattca gcccatatcg tttcatagct tctgccaacc gaacggacat ttcgaagtat 2400 tccgcgtacg tgatgttcac ctcgatatgt gcatctgtaa aagcaattgt tccaggaacc 2460 agggcgtatc tcttcatagc cttatgcagt tgctctccag cggttccatc ctctagagga 2520 tagaatggcg ccgggccttt ctttatgttt ttggcgtctt ccattttacc aacagtaccg 2580 gaatgccaag ctcgagctca gggtcattaa ttgtaaagga aaggtgccga cctccggtca 2640 cctattcagg cgtagaagct ttttaaaccg agcgctttta tagaagcccc agtccccttc 2700 tcagatcccg agtgtcgcgt gttacctcgg ggtacctgaa gggcatcctt agcctgtcac 2760 cagtgtgtgg gtaccagttt caatgtcact ttaaagtggg tttcgcagta aagtcgccgt 2820 gctggggttg cacacatgac gggcccgtgt gggcgtggct tttggccccg tgggtccttg 2880 ttaccaagga ggagttccca cggccgtcat gctccgctac gaagcaacag tgctgttact 2940 aacactcgcc agtggatcga gcgtggagcc aaacacagta caaagtgtta cccctctaga 3000 cctggaaaga ccaggcggag tttcaagttg cacgttttgt gtcagttggg gaaacctgct 3060 gagctctgcg gcacgctgtt gacgctgtta agcgggtcgc tgcagcaata actgccttaa 3120 aaaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg 3180 ccgacatgga agccatcaca gacggcatga tgaacctgaa tcgccagcgg catcagcacc 3240 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata 3300 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac 3360 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct 3420 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa 3480 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc 3540 agctcaccgt ctttcattgc catacggaat tccggatgag cattcatcag gcgggcaaga 3600 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc 3660 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca 3720 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc 3780 tccattttag cttctgcagg gtcgctcggt gttcgaggcc acacgcgtca ccttaatatg 3840 cgaagtggac ctcggaccgc gccgccccga ctgcatctgc gtgttcgaat tcgccaatga 3900 caagacgctg ggcggggttt gccggatcct ctagagtcga gcttgggtct ccctatagtg 3960 agtcgtatta atttcgataa gccagctgca ttaatgaatc ggccaacgcg cggggagagg 4020 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 4080 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 4140 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 4200 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 4260 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 4320 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 4380 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 4440 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 4500 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 4560 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 4620 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 4680 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 4740 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 4800 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 4860 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 4920 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 4980 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 5040 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 5100 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 5160 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 5220 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 5280 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 5340 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 5400 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 5460 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 5520 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 5580 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 5640 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 5700 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 5760 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 5820 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 5880 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 5940 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac 6000 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga 6060 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga 6120 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg 6180 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatcg acgctctccc 6240 ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc 6300 gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct 6360 gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc 6420 ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgcc 6479 //