LOCUS       pCAGGSMtCAT             6430 bp    DNA     circular     29-MAR-2006
DEFINITION  LMBP 3482.
SOURCE      
  ORGANISM  
COMMENT     29/03/06 MTS aangepast na sequencing: GCT (Ala) -> GTT (Val)
COMMENT     VNTNAME|pCAGGSMtCAT|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGSMtCAT" 0 6430 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 "proto011" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "BamHI" "EcoRI" "EcoRV" "HindIII" "NcoI" "SalI" "XbaI" 
COMMENT         "XhoI") (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") 
COMMENT        (CObList) 1 "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 
COMMENT        0 (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 6430 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 4 580 0 643 0 2370 0 2707 
COMMENT            0 0 "") #1=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 6429 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 2186 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 2 2463 0 5079 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "SalI" "gtcgac" 2 0 1 4701 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 6333 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 1254 0 0 "")) 
COMMENT       (CObList 
COMMENT        #8=(CFSignal (CObList) "\337-globin polyA" 43 0 0 1720 1811 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #9=(CFSignal (CObList) "lac promoter" 30 0 1 2246 2328 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #10=(CFSignal (CObList) "SV40 ori" 33 0 0 2470 2545 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #11=(CFSignal (CObList) "pMB1 ori" 33 0 1 2765 3166 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #12=(CFSignal (CObList) "AMP" 4 0 1 3707 4567 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #13=(CFSignal (CObList) "intron AG promoter" 15 0 0 5455 6379 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #14=(CFSignal (CObList) "3' FR of \337-globin" 21 0 0 1812 2168 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #15=(CFSignal (CObList) "SV40 polyA early" 43 0 0 2576 2650 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #16=(CFSignal (CObList) "SV40 polyA late" 43 0 1 2576 2650 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #17=(CFSignal (CObList) "AG promoter" 29 0 0 5086 6427 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #18=(CFSignal (CObList) "hCMV enhancer" 29 0 0 4703 5080 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #19=(CFSignal (CObList) "rCATf" 4 0 0 112 478 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #20=(CFSignal (CObList) "hCATf" 4 0 0 479 1644 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #21=(CFSignal (CObList) "MTS of hMnSOD" 4 0 0 40 111 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #22=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #23=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "pCAGGSMtCAT" 2) 5 
COMMENT                   "" 0 4) 
COMMENT              #24=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'pCAGGSMtCAT'" 1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 3482" 
COMMENT                          1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: Demo User" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Creation Date: ? (no data)" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 03/29/06 04:14" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6430 bp" 1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #33=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) (CObjectList)) 
COMMENT                   "Standard Fields") 
COMMENT              #34=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 5 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Demo User" 
COMMENT                          1))) "Original Author") 
COMMENT              #36=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #37=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) 
COMMENT                   "Annotations") 
COMMENT              #38=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #39=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #40=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 40 (3 #21# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #41=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 40    End: 111 " 1) 
COMMENT                                   #42=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "mitochondrial targeting sequence of the hMnSOD gene" 
COMMENT                                        1))) "MTS of hMnSOD") 
COMMENT                            #43=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 112 (3 #19# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #44=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 112   End: 478 " 1) 
COMMENT                                   #45=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "fragment of the rat catalase mature sequence" 
COMMENT                                        1))) "rCATf") 
COMMENT                            #46=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 479 (3 #20# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #47=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 479   End: 1644" 1) 
COMMENT                                   #48=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "fragment of the human catalase mature sequence" 
COMMENT                                        1))) "hCATf") 
COMMENT                            #49=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3707 (3 #12# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #50=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3707  End: 4567 (Complementary)" 
COMMENT                                        1))) "AMP"))) "CDS (4 total)") 
COMMENT                     #51=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 15 (7 15 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #52=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5455 (3 #13# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #53=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5455  End: 6379" 1))) 
COMMENT                                 "intron AG promoter"))) "Intron (1 total)") 
COMMENT                     #54=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #55=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1812 (3 #14# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #56=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1812  End: 2168" 1))) 
COMMENT                                 "3' FR of \337-globin"))) 
COMMENT                          "Misc. Feature (1 total)") 
COMMENT                     #57=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #58=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4703 (3 #18# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #59=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4703  End: 5080" 1))) 
COMMENT                                 "hCMV enhancer") 
COMMENT                            #60=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5086 (3 #17# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #61=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5086  End: 6427" 1) 
COMMENT                                   #62=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "chicken \337-actin/rabbit \337-globin hybrid promoter" 
COMMENT                                        1))) "AG promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
COMMENT                     #63=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #64=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2246 (3 #9# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #65=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2246  End: 2328 (Complementary)" 
COMMENT                                        1))) "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
COMMENT                     #66=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #67=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2470 (3 #10# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #68=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2470  End: 2545" 1))) "SV40 ori") 
COMMENT                            #69=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2765 (3 #11# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #70=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2765  End: 3166 (Complementary)" 
COMMENT                                        1))) "pMB1 ori"))) 
COMMENT                          "Replication Origin (2 total)") 
COMMENT                     #71=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 43 (7 43 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #72=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1720 (3 #8# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1720  End: 1811" 1))) 
COMMENT                                 "\337-globin polyA") 
COMMENT                            #74=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2576 (3 #15# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #75=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2576  End: 2650" 1))) 
COMMENT                                 "SV40 polyA early") 
COMMENT                            #76=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2576 (3 #16# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2576  End: 2650 (Complementary)" 
COMMENT                                        1))) "SV40 polyA late"))) 
COMMENT                          "Terminator (3 total)"))) "Feature Map") 
COMMENT              #78=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #79=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 35424544 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #80=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #0# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #81=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #0# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 580 " 1) 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #0# 2) 1 2 2 0 191) 
COMMENT                                        " N2: 643 " 1) 
COMMENT                                   #83=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #0# 3) 1 2 2 0 191) 
COMMENT                                        " N3: 2370 " 1) 
COMMENT                                   #84=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #0# 4) 1 2 2 0 191) 
COMMENT                                        " N4: 2707 " 1))))) "BamHI: 4 sites") 1 
COMMENT                          5 "GGATCC" "CCTAGG") 
COMMENT                     #85=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 35413816 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #86=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #1# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #87=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #1# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 6429 " 1))))) "EcoRI: 1 site") 1 5 
COMMENT                          "GAATTC" "CTTAAG") 
COMMENT                     #88=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 27476088 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList)) "EcoRV: 0 sites") 3 3 "GATATC" 
COMMENT                          "CTATAG") 
COMMENT                     #89=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 27503544 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #90=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #3# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #91=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #3# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 2186 " 1))))) "HindIII: 1 site") 1 
COMMENT                          5 "AAGCTT" "TTCGAA") 
COMMENT                     #92=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 35424704 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #93=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #4# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #94=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #4# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 2463 " 1) 
COMMENT                                   #95=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #4# 2) 1 2 2 0 191) 
COMMENT                                        " N2: 5079 " 1))))) "NcoI: 2 sites") 1 5 
COMMENT                          "CCATGG" "GGTACC") 
COMMENT                     #96=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 35424624 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #97=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #5# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #98=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #5# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 4701 " 1))))) "SalI: 1 site") 1 5 
COMMENT                          "GTCGAC" "CAGCTG") 
COMMENT                     #99=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 27462296 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #100=(CGroupPar 
COMMENT                                   (CParagraph 0 (10 #6# 0) 1 2 2 0 180) 
COMMENT                                   (CObjectList 
COMMENT                                    #101=(CLinePar 
COMMENT                                          (CParagraph 0 (1 #6# 1) 1 2 2 0 191) 
COMMENT                                          " N1: 6333 " 1))))) "XbaI: 1 site") 1 
COMMENT                          5 "TCTAGA" "AGATCT") 
COMMENT                     #102=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 27502664 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #103=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #7# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #104=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #7# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 1254 " 1))))) "XhoI: 1 site") 1 
COMMENT                           5 "CTCGAG" "GAGCTC"))) "Restriction/Methylation Map")))) 
COMMENT       (CGraphView 
COMMENT        (CWStyleSheet 
COMMENT         (CObjectList 
COMMENT          #105=(CWidgetStyle "Annotation" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                0 0) 
COMMENT          #106=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 5 "@N (@S)" 0) 
COMMENT          #107=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 
COMMENT                1 2 "?" 0) 
COMMENT          #108=(CWidgetStyle "Mark 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" 
COMMENT                0) 
COMMENT          #109=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.64 0 1 -1 
COMMENT                "@N (@H%)" 0) 
COMMENT          #110=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 5 "@N (@S)" 0) 
COMMENT          #111=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #112=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 
COMMENT                "@F bp (molecule @L bp)" 0) 
COMMENT          #113=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 
COMMENT                "Fragment of @N" 0) 
COMMENT          #114=(CWidgetStyle "Molecule Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 16 
COMMENT                "@L bp" 0) 
COMMENT          #115=(CWidgetStyle "Molecule Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 "@N" 0) 
COMMENT          #116=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 2 8388608 5) 0 0 0) 
COMMENT          #117=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 255 0) 0 
COMMENT                0 0) 
COMMENT          #118=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 
COMMENT                0 0) 
COMMENT          #119=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 0 0 0) 
COMMENT          #120=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 
COMMENT                0) 
COMMENT          #121=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 0) 2 1 (LOGBRUSH 0 12632256 0) 
COMMENT                0 0 0) #122=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #123=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) 
COMMENT          #124=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #125=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #126=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) 
COMMENT          #127=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) 
COMMENT          #128=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #129=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #130=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 8388608) 10 0 0 0 0) 
COMMENT          #131=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #132=(CWidgetStyle "Void" 0 0 0 0 0) 
COMMENT          #133=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                1 3 "@T @N " 0) 
COMMENT          #134=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #135=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #136=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #137=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0)) 0.164644 1.74233 0.164644 2.53336 
COMMENT         (15 (CShapeMapEntry 0 "Line 1" 1 "Position Label") 9 
COMMENT          (CShapeMapEntry 0 "Shape 4" 1 "Position Label") 4 
COMMENT          (CShapeMapEntry 0 "Shape 3" 1 "Position Label") 30 
COMMENT          (CShapeMapEntry 0 "Shape 1" 1 "Position Label") 29 
COMMENT          (CShapeMapEntry 0 "Shape 1" 1 "Position Label") 26 
COMMENT          (CShapeMapEntry 0 "Line 0" 1 "Position Label") 25 
COMMENT          (CShapeMapEntry 0 "Line 4" 1 "Position Label") 22 
COMMENT          (CShapeMapEntry 0 "Shape 2" 1 "Position Label") 21 
COMMENT          (CShapeMapEntry 5 "Line 2" 1 "Position Label") 43 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Position Label") 33 
COMMENT          (CShapeMapEntry 0 "Shape 4" 1 "Position Label") 32 
COMMENT          (CShapeMapEntry 0 "Line 3" 1 "Position Label") 52 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Position Label")) 39 39 39 39 0.1 0) 1 1 
COMMENT        0 1 1 (mapper: 20.1769 -16.2469 39 39 0.01 10 -1 6430 6430 1 0 0) 
COMMENT        #138=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil 36486920 100) 
COMMENT              (CObjectList 
COMMENT               #139=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -1332 100) 
COMMENT                     (CObjectList 
COMMENT                      #140=(CAxis 
COMMENT                            (CWideLine 
COMMENT                             (CWidget 0 (0 0) 1 2 0 0 #121# 35916676 0) 
COMMENT                             (LOGPEN 0 0 0) 2 (LOGBRUSH 0 12632256 0) 1 6.27516 
COMMENT                             6.27316 1 0.0214037) 0.0975612) 
COMMENT                      #141=(CLabel 
COMMENT                            (CWidget 1001 (0 0) 1 2 0 0 #115# 35210948 100) 
COMMENT                            (LOGPEN 0 0 8388608) 1 
COMMENT                            (LOGFONT 61 23 0 0 400 0 0 0 0 3 2 1 34 "Arial") 
COMMENT                            1.74233 0.91 0 "pCAGGSMtCAT" "@N" 1 0 0.871165 0 -10 
COMMENT                            9.46154 1.51282 Nil) 
COMMENT                      #142=(CLabel 
COMMENT                            (CWidget 1002 (0 0) 1 2 0 0 #114# 35263812 100) 
COMMENT                            (LOGPEN 0 0 8388608) 1 
COMMENT                            (LOGFONT 70 26 0 0 400 0 0 0 0 3 2 1 49 
COMMENT                             "Courier New") 1.74233 1.05 0 "6430 bp" "@L bp" 16 
COMMENT                            0 -0.871165 0 -10.8 4.64103 1.76923 Nil)) 
COMMENT                     (CObjectList)) 
COMMENT               #143=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -992 100) 
COMMENT                     (CObjectList 
COMMENT                      #144=(CGroupWidget 
COMMENT                            (CWidget 4 (7 4 0) 1 2 0 0 Nil -1060 100) 
COMMENT                            (CObjectList 
COMMENT                             #145=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #12# 0) 1 2 0 0 #117# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    1.81757 2.65757 1 0.082322) 0.8 1.8 1) 
COMMENT                             #146=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #135# 36493756 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 42 15 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.63 8388608 "AMP" "@N" 1 
COMMENT                                   -11.0855 -6.96932 0 -10 1.94872 1.05128 #145#) 
COMMENT                             #147=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #19# 0) 1 2 0 0 #117# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    5.80684 6.16489 1 0.082322) 0.8 1.8 0) 
COMMENT                             #148=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #135# 27495652 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 42 15 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.63 8388608 "rCATf" "@N" 1 
COMMENT                                   7.70185 10.454 0 -10 2.25641 1.05128 #147#) 
COMMENT                             #149=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #20# 0) 1 2 0 0 #117# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    4.66928 5.80684 1 0.082322) 0.8 1.8 0) 
COMMENT                             #150=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #135# 35222708 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 42 15 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.63 8388608 "hCATf" "@N" 1 
COMMENT                                   12.2897 5.22699 0 -10 2.51282 1.05128 #149#) 
COMMENT                             #151=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #21# 0) 1 2 0 0 #117# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    6.16489 6.23514 1 0.082322) 0.8 1.8 0) 
COMMENT                             #152=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #135# 35417516 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 42 15 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.63 8388608 
COMMENT                                   "MTS of hMnSOD" "@N" 1 5.16708 12.1963 0 -10 
COMMENT                                   6.58974 1.05128 #151#)) (CObjectList)) 
COMMENT                      #153=(CGroupWidget 
COMMENT                            (CWidget 15 (7 15 0) 1 2 0 0 Nil -517 100) 
COMMENT                            (CObjectList 
COMMENT                             #154=(CLine 
COMMENT                                   (CWidget 0 (3 #13# 0) 1 2 0 0 #126# 
COMMENT                                    1816181360 100) (LOGPEN 0 0 16711680) 1 
COMMENT                                   0.835356 0.0497562 0.952197) 
COMMENT                             #155=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #135# 35297724 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 42 15 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.63 8388608 
COMMENT                                   "intron AG promoter" "@N" 1 -10.3153 10.454 0 
COMMENT                                   -10 7.30769 1.05128 #154#)) (CObjectList)) 
COMMENT                      #156=(CGroupWidget 
COMMENT                            (CWidget 21 (7 21 0) 1 2 0 0 Nil -177 100) 
COMMENT                            (CObjectList 
COMMENT                             #157=(CLine 
COMMENT                                   (CWidget 0 (3 #14# 0) 1 2 0 0 #125# 0 100) 
COMMENT                                   (LOGPEN 0 6 16711680) 8 0.835356 4.15806 
COMMENT                                   4.50635) 
COMMENT                             #158=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #135# 27664020 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 42 15 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.63 8388608 
COMMENT                                   "3' FR of \337-globin" "@N" 1 14.9002 
COMMENT                                   -3.48466 0 -10 6.35897 1.05128 #157#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #159=(CGroupWidget 
COMMENT                            (CWidget 29 (7 29 0) 1 2 0 0 Nil -721 100) 
COMMENT                            (CObjectList 
COMMENT                             #160=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #17# 0) 1 2 0 0 #119# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 8388736 0) 1 
COMMENT                                    0.00292684 1.3122 1 0.082322) 0.8 1.8 0) 
COMMENT                             #161=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #135# 35149964 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 42 15 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.63 8388608 "AG promoter" 
COMMENT                                   "@N" 1 -11.2978 8.71165 0 -10 4.92308 1.05128 
COMMENT                                   #160#) 
COMMENT                             #162=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #18# 0) 1 2 0 0 #119# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 8388736 0) 1 
COMMENT                                    1.31708 1.68586 1 0.082322) 0.8 1.8 0) 
COMMENT                             #163=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #135# 36500780 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 42 15 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.63 8388608 
COMMENT                                   "hCMV enhancer" "@N" 1 -15.2687 0 0 -10 
COMMENT                                   6.20513 1.05128 #162#)) (CObjectList)) 
COMMENT                      #164=(CGroupWidget 
COMMENT                            (CWidget 30 (7 30 0) 1 2 0 0 Nil -312 100) 
COMMENT                            (CObjectList 
COMMENT                             #165=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #9# 0) 1 2 0 0 #119# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 8388736 0) 1 
COMMENT                                    4.00196 4.08294 1 0.082322) 0.8 1.8 1) 
COMMENT                             #166=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #135# 36534364 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 42 15 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.63 8388608 "lac promoter" 
COMMENT                                   "@N" 1 12.61 -6.96932 0 -10 4.82051 1.05128 
COMMENT                                   #165#)) (CObjectList)) 
COMMENT                      #167=(CGroupWidget 
COMMENT                            (CWidget 33 (7 33 0) 1 2 0 0 Nil -516 100) 
COMMENT                            (CObjectList 
COMMENT                             #168=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #10# 0) 1 2 0 0 #116# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 1 
COMMENT                                    3.79025 3.8644 1 0.082322) 0.8 1.8 0) 
COMMENT                             #169=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #135# 27651084 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 42 15 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.63 8388608 "SV40 ori" 
COMMENT                                   "@N" 1 10.1528 -12.1963 0 -10 3.38462 1.05128 
COMMENT                                   #168#) 
COMMENT                             #170=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #11# 0) 1 2 0 0 #116# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 1 
COMMENT                                    3.1844 3.57659 1 0.082322) 0.8 1.8 1) 
COMMENT                             #171=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #135# 35155936 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 42 15 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.63 8388608 "pMB1 ori" 
COMMENT                                   "@N" 1 5.78811 -19.1656 0 -10 3.53846 1.05128 
COMMENT                                   #170#)) (CObjectList)) 
COMMENT                      #172=(CGroupWidget 
COMMENT                            (CWidget 43 (7 43 0) 1 2 0 0 Nil -380 100) 
COMMENT                            (CObjectList 
COMMENT                             #173=(CLine 
COMMENT                                   (CWidget 0 (3 #8# 0) 1 2 0 0 #125# 36486920 
COMMENT                                    100) (LOGPEN 0 6 16711680) 8 0.835356 
COMMENT                                   4.50635 4.59611) 
COMMENT                             #174=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #135# 35133932 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 42 15 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.63 8388608 
COMMENT                                   "\337-globin polyA" "@N" 1 14.7831 -1.74233 0 
COMMENT                                   -10 5.64103 1.05128 #173#) 
COMMENT                             #175=(CLine 
COMMENT                                   (CWidget 0 (3 #15# 0) 1 2 0 0 #125# 36147544 
COMMENT                                    100) (LOGPEN 0 6 16711680) 8 0.835356 
COMMENT                                   3.68781 3.76098) 
COMMENT                             #176=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #135# 27615876 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 42 15 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.63 8388608 
COMMENT                                   "SV40 polyA early" "@N" 1 10.9922 -13.9386 0 
COMMENT                                   -10 6.66667 1.05128 #175#) 
COMMENT                             #177=(CLine 
COMMENT                                   (CWidget 0 (3 #16# 0) 1 2 0 0 #125# 641 100) 
COMMENT                                   (LOGPEN 0 6 16711680) 8 0.670712 3.68781 
COMMENT                                   3.76098) 
COMMENT                             #178=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #135# 18088352 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 42 15 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.63 8388608 
COMMENT                                   "SV40 polyA late" "@N" 1 10.7896 -15.681 0 
COMMENT                                   -10 6.28205 1.05128 #177#)) (CObjectList))) 
COMMENT                     (CObjectList)) 
COMMENT               #179=(CGroupWidget (CWidget 11 (8 0) 1 2 0 0 Nil -857 100) 
COMMENT                     (CObjectList 
COMMENT                      #180=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #1# 0) 1 2 0 0 Nil -1265 100) 
COMMENT                            (CObjectList 
COMMENT                             #181=(CScratch 
COMMENT                                   (CWidget 1 (1 #1# 1) 1 2 0 0 #128# 880 100) 
COMMENT                                   (LOGPEN 0 6 0) 8 1 0.00195122 1.9 0.082322 1) 
COMMENT                             #182=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #111# 35149964 100) 
COMMENT                                   (LOGPEN 0 0 32896) 1 
COMMENT                                   (LOGFONT 41 15 0 0 400 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.611111 0 "EcoRI" "@N" 1 
COMMENT                                   -2.19434 13.9386 0 -10 2.35897 1.05128 #181#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #183=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #3# 0) 1 2 0 0 Nil 525 100) 
COMMENT                            (CObjectList 
COMMENT                             #184=(CScratch 
COMMENT                                   (CWidget 1 (1 #3# 1) 1 2 0 0 #128# 380 100) 
COMMENT                                   (LOGPEN 0 6 0) 8 1 4.14147 1.9 0.082322 1) 
COMMENT                             #185=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #111# 36497740 100) 
COMMENT                                   (LOGPEN 0 0 32896) 1 
COMMENT                                   (LOGFONT 41 15 0 0 400 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.611111 0 "HindIII" "@N" 1 
COMMENT                                   12.342 -5.22699 0 -10 2.58974 1.05128 #184#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #186=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #5# 0) 1 2 0 0 Nil 461 100) 
COMMENT                            (CObjectList 
COMMENT                             #187=(CScratch 
COMMENT                                   (CWidget 1 (1 #5# 1) 1 2 0 0 #128# 938 100) 
COMMENT                                   (LOGPEN 0 6 0) 8 1 1.68781 1.9 0.082322 1) 
COMMENT                             #188=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #111# 35084492 100) 
COMMENT                                   (LOGPEN 0 0 32896) 1 
COMMENT                                   (LOGFONT 41 15 0 0 400 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.611111 0 "SalI" "@N" 1 
COMMENT                                   -12.7379 -1.74233 0 -10 1.61538 1.05128 #187#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #189=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #6# 0) 1 2 0 0 Nil -721 100) 
COMMENT                            (CObjectList 
COMMENT                             #190=(CScratch 
COMMENT                                   (CWidget 1 (1 #6# 1) 1 2 0 0 #128# 35151624 
COMMENT                                    100) (LOGPEN 0 6 0) 8 1 0.09561 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #191=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #111# 35111540 100) 
COMMENT                                   (LOGPEN 0 0 32896) 1 
COMMENT                                   (LOGFONT 41 15 0 0 400 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.611111 0 "XbaI" "@N" 1 
COMMENT                                   -2.89362 12.1963 0 -10 1.87179 1.05128 #190#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #192=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #7# 0) 1 2 0 0 Nil 461 100) 
COMMENT                            (CObjectList 
COMMENT                             #193=(CScratch 
COMMENT                                   (CWidget 1 (1 #7# 1) 1 2 0 0 #128# 36421024 
COMMENT                                    100) (LOGPEN 0 6 0) 8 1 5.05074 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #194=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #111# 18088368 100) 
COMMENT                                   (LOGPEN 0 0 32896) 1 
COMMENT                                   (LOGFONT 41 15 0 0 400 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.611111 0 "XhoI" "@N" 1 
COMMENT                                   12.5307 3.48466 0 -10 1.87179 1.05128 #193#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #195=(CGroupWidget 
COMMENT                            (CWidget 2 (10 #4# 0) 1 2 0 0 Nil -1197 100) 
COMMENT                            (CObjectList 
COMMENT                             #196=(CScratch 
COMMENT                                   (CWidget 1 (1 #4# 1) 1 2 0 0 #128# 938 100) 
COMMENT                                   (LOGPEN 0 6 0) 8 1 3.87123 1.9 0.082322 1) 
COMMENT                             #197=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #110# 27557500 100) 
COMMENT                                   (LOGPEN 0 0 8421376) 1 
COMMENT                                   (LOGFONT 41 15 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 0 
COMMENT                                   "NcoI (2463)" "@N (@S)" 5 11.097 -10.454 0 
COMMENT                                   -10 3.94872 0.974359 #196#) 
COMMENT                             #198=(CScratch 
COMMENT                                   (CWidget 2 (1 #4# 2) 1 2 0 0 #128# 36448840 
COMMENT                                    100) (LOGPEN 0 6 0) 8 1 1.31903 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #199=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #110# 35766228 100) 
COMMENT                                   (LOGPEN 0 0 8421376) 1 
COMMENT                                   (LOGFONT 41 15 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 0 
COMMENT                                   "NcoI (5079)" "@N (@S)" 5 -14.1697 3.48466 0 
COMMENT                                   -10 3.94872 0.974359 #198#)) (CObjectList)) 
COMMENT                      #200=(CGroupWidget 
COMMENT                            (CWidget 4 (10 #0# 0) 1 2 0 0 Nil -110 100) 
COMMENT                            (CObjectList 
COMMENT                             #201=(CScratch 
COMMENT                                   (CWidget 1 (1 #0# 1) 1 2 0 0 #128# 938 100) 
COMMENT                                   (LOGPEN 0 6 0) 8 1 5.70831 1.9 0.082322 1) 
COMMENT                             #202=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #110# 28239428 100) 
COMMENT                                   (LOGPEN 0 0 8421376) 1 
COMMENT                                   (LOGFONT 41 15 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 0 
COMMENT                                   "BamHI (580)" "@N (@S)" 5 11.4024 8.71165 0 
COMMENT                                   -10 4.35897 0.974359 #201#) 
COMMENT                             #203=(CScratch 
COMMENT                                   (CWidget 2 (1 #0# 2) 1 2 0 0 #128# 380 100) 
COMMENT                                   (LOGPEN 0 6 0) 8 1 5.64684 1.9 0.082322 1) 
COMMENT                             #204=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #110# 35363988 100) 
COMMENT                                   (LOGPEN 0 0 8421376) 1 
COMMENT                                   (LOGFONT 41 15 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 0 
COMMENT                                   "BamHI (643)" "@N (@S)" 5 12.7756 6.96932 0 
COMMENT                                   -10 4.35897 0.974359 #203#) 
COMMENT                             #205=(CScratch 
COMMENT                                   (CWidget 3 (1 #0# 3) 1 2 0 0 #128# 879 100) 
COMMENT                                   (LOGPEN 0 6 0) 8 1 3.96196 1.9 0.082322 1) 
COMMENT                             #206=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #110# 36622300 100) 
COMMENT                                   (LOGPEN 0 0 8421376) 1 
COMMENT                                   (LOGFONT 41 15 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 0 
COMMENT                                   "BamHI (2370)" "@N (@S)" 5 12.1879 -8.71165 0 
COMMENT                                   -10 4.74359 0.974359 #205#) 
COMMENT                             #207=(CScratch 
COMMENT                                   (CWidget 4 (1 #0# 4) 1 2 0 0 #128# 36443688 
COMMENT                                    100) (LOGPEN 0 6 0) 8 1 3.63318 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #208=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #110# 36533580 100) 
COMMENT                                   (LOGPEN 0 0 8421376) 1 
COMMENT                                   (LOGFONT 41 15 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 0 
COMMENT                                   "BamHI (2707)" "@N (@S)" 5 9.64415 -17.4233 0 
COMMENT                                   -10 4.74359 0.974359 #207#)) (CObjectList))) 
COMMENT                     (CObjectList)) 
COMMENT               #209=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -1401 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #210=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -1129 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 6 (CObjectList) (CObList) 1 (CObList)) (CObList) 909324854 
COMMENT       (CStringList) 1952545872 2083607156 (CObList)))
FEATURES             Location/Qualifiers
     terminator      1720..1811
                     /vntifkey="43"
                     /label=ß-globin\polyA
     promoter        complement(2246..2328)
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      2470..2545
                     /vntifkey="33"
                     /label=SV40\ori
     rep_origin      complement(2765..3166)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(3707..4567)
                     /vntifkey="4"
                     /label=AMP
     intron          5455..6379
                     /vntifkey="15"
                     /label=intron\AG\promoter
     misc_feature    1812..2168
                     /vntifkey="21"
                     /label=3'\FR\of\ß-globin
     terminator      2576..2650
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(2576..2650)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     promoter        5086..6427
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     promoter        4703..5080
                     /vntifkey="29"
                     /label=hCMV\enhancer
     CDS             112..478
                     /vntifkey="4"
                     /label=rCATf
                     /note="fragment of the rat catalase mature sequence"
     CDS             479..1644
                     /vntifkey="4"
                     /label=hCATf
                     /note="fragment of the human catalase mature sequence"
     CDS             40..111
                     /vntifkey="4"
                     /label=MTS\of\hMnSOD
                     /note="mitochondrial targeting sequence of the hMnSOD gene"
BASE COUNT     1424 a      1692 c      1748 g      1566 t 
ORIGIN
        1 ttcggcggcg gcatcagcgg tagcaccagc actagcagca tgttgagccg ggcagtgtgc 
       61 ggcaccagca ggcagctggc tccggttttg gggtatctgg gctccaggca gatggcggac 
      121 agccgggacc cagccagcga ccagatgaag cagtggaagg agcagcgggc ccctcagaaa 
      181 cccgatgtcc tgaccaccgg aggcgggaac ccaataggag ataaacttaa tatcatgact 
      241 gcggggcccc gagggcccct cctcgttcaa gatgtggttt tcaccgacga gatggcacac 
      301 tttgacagag agcggattcc tgagagagtg gtacatgcaa agggagcagg tgcttttgga 
      361 tactttgagg tcacccacga tattaccaga tactccaagg caaaggtgtt tgagcatatt 
      421 gggaagagga ctcctattgc cgtccgattc tccacagtcg ctggagagtc aggctcagct 
      481 gacacagttc gggaccctcg tgggtttgca gtgaaatttt acacagaaga tggtaactgg 
      541 gatctcgttg gaaataacac ccccattttc ttcatcaggg atcccatatt gtttccatct 
      601 tttatccaca gccaaaagag aaatcctcag acacatctga aggatccgga catggtctgg 
      661 gacttctgga gcctacgtcc tgagtctctg catcaggttt ctttcttgtt cagtgatcgg 
      721 gggattccag atggacatcg ccacatgaat ggatatggat cacatacttt caagctggtt 
      781 aatgcaaatg gggaggcagt ttattgcaaa ttccattata agactgacca gggcatcaaa 
      841 aacctttctg ttgaagatgc ggcgagactt tcccaggaag atcctgacta tggcatccgg 
      901 gatcttttta acgccattgc cacaggaaag tacccctcct ggacttttta catccaggtc 
      961 atgacattta atcaggcaga aacttttcca tttaatccat tcgatctcac caaggtttgg 
     1021 cctcacaagg actaccctct catcccagtt ggtaaactgg tcttaaaccg gaatccagtt 
     1081 aattactttg ctgaggttga acagatagcc ttcgacccaa gcaacatgcc acctggcatt 
     1141 gaggccagtc ctgacaaaat gcttcagggc cgcctttttg cctatcctga cactcaccgc 
     1201 catcgcctgg gacccaatta tcttcatata cctgtgaact gtccctaccg tgctcgagtg 
     1261 gccaactacc aacgtgacgg cccgatgtgc atgcaggaca atcagggtgg tgctccaaat 
     1321 tactacccca acagctttgg tgctccggaa caacagcctt ctgccttgga gcacagcatc 
     1381 caatattctg gagaagtgcg gagattcaac actgccaatg atgataacgt tactcaggtg 
     1441 cgggcattct atgtgaacgt gctgaatgag gaacagagga aacgtctgtg tgagaacatt 
     1501 gccggccacc tgaaggatgc acaaattttc atccagaaga aagcggtcaa gaacttcact 
     1561 gaggtccacc ctgactacgg gagccacatc caggctcttc tggacaagta caatgctgag 
     1621 aagcctaaga atgcgattca cacctgacct caggtgcagg ctgcctatca gaaggtggtg 
     1681 gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa 
     1741 aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt 
     1801 attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg 
     1861 agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc 
     1921 catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag 
     1981 ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta gatttttttt 
     2041 atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta 
     2101 ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta 
     2161 tggagatccc tcgacctgca gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt 
     2221 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 
     2281 agcctgggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct 
     2341 ttccagtcgg gaaacctgtc gtgccagcgg atccgcatct caattagtca gcaaccatag 
     2401 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 
     2461 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc 
     2521 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctaactt 
     2581 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 
     2641 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 
     2701 tgtctggatc cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt 
     2761 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 
     2821 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg gataacgcag 
     2881 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 
     2941 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 
     3001 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 
     3061 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 
     3121 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 
     3181 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 
     3241 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 
     3301 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 
     3361 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc 
     3421 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 
     3481 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 
     3541 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 
     3601 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 
     3661 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 
     3721 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 
     3781 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 
     3841 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 
     3901 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 
     3961 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 
     4021 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 
     4081 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 
     4141 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 
     4201 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 
     4261 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 
     4321 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 
     4381 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 
     4441 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 
     4501 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 
     4561 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 
     4621 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 
     4681 cccgaaaagt gccacctggg tcgacattga ttattgacta gttattaata gtaatcaatt 
     4741 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat 
     4801 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 
     4861 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 
     4921 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 
     4981 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 
     5041 acttggcagt acatctacgt attagtcatc gctattacca tggtcgaggt gagccccacg 
     5101 ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta tttatttatt 
     5161 ttttaattat tttgtgcagc gatgggggcg gggggggggg gggggccccc cccaggcggg 
     5221 gcggggcggg gcgaggggcg gggcggggcg aggcggaaag gtgcggcggc agccaatcag 
     5281 agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa 
     5341 aagcgaagcg cgcggcgggc gggagtcgtt gcgcgctgcc ttccccccgt gccccgctcc 
     5401 gccgccgcct cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc 
     5461 gggcgggacg gcccttctcc tccgggctgt aattagcgct tggtttaatg acggcttgtt 
     5521 tcttttctgt ggctgcgtga aagccttgag gggctccggg agggcccttt gtgcgggggg 
     5581 agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg 
     5641 ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg 
     5701 cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac 
     5761 aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc 
     5821 gggctgcaac cccccctgca cccccctccc cgagttgctg agcacggccc ggcttcgggt 
     5881 gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag 
     5941 gtgggggtgc cgggcggggc ggggccgcct cgggccgggg agggctcggg ggaaggggcg 
     6001 cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt 
     6061 atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa 
     6121 atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg 
     6181 caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc 
     6241 tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc 
     6301 ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc 
     6361 cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt 
     6421 tggcaaagaa 
//
