LOCUS       pATmIL6hIFNG            5856 bp    DNA     circular     23-OCT-2007
DEFINITION  LMBP 3665.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 3665
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium).
            Last modified: 23-OCT-2007
            
COMMENT     VNTNAME|pATmIL6hIFNG|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pATmIL6hIFNG" 0 5856 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43) 
COMMENT        (CWordArray) (CStringList "BsaI" "HindIII" "XhoI" "XbaI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 5856 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "BsaI" "ggtctc" 8 12 2 874 1 4625 1 0 "") 
COMMENT        #1=(CRSite (CStringList) "HindIII" "aagctt" 2 0 2 2 0 1408 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 536 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "XbaI" "tctaga" 2 0 2 26 0 1417 0 0 "")) 
COMMENT       (CObList 
COMMENT        #4=(CFSignal (CObList) "mIL6 promoter" 29 0 0 42 519 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "42..519") 
COMMENT        #5=(CFSignal (CObList) "hIFNG" 4 0 0 600 1100 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "600..1100") 
COMMENT        #6=(CFSignal (CObList) "part of SV40 small t-antigen" 4 0 0 1429 1503 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "1429..1503") 
COMMENT        #7=(CFSignal (CObList) "SV40 polyA early" 43 0 0 2123 2219 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "2123..2219") 
COMMENT        #8=(CFSignal (CObList) "SV40 polyA late" 43 0 1 2123 2219 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(2123..2219)") 
COMMENT        #9=(CFSignal (CObList) "pMB1 ori" 33 0 1 3547 3949 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(3547..3949)") 
COMMENT        #10=(CFSignal (CObList) "ampicillin" 4 0 1 4490 5350 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(4490..5350)") 
COMMENT        #11=(CFSignal (CObList) "SV40 polyA early" 43 0 0 5482 5556 0 
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COMMENT        #12=(CFSignal (CObList) "SV40 polyA late" 43 0 1 5482 5556 0 
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COMMENT        #14=(CFSignal (CObList) "SV40 polyA late" 43 0 1 5697 5793 0 
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COMMENT        #15=(CFSignal (CObList) "intron of SV40 small t-antigen" 15 0 0 1504 
COMMENT             1570 0 (CStringList) (CStringList) 1 1 1 1 "1504..1570") 
COMMENT        #16=(CFSignal (CObList) "3' UTR of SV40 small t-antigen" 21 0 0 1571 
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COMMENT        #17=(CFSignal (CObList) "LMBP 3665" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #18=(CFSignal (CObList) "Last modified: 23-OCT-2007" 2 0 0 0 0 0 
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COMMENT                          "DNA Plasmid 'pATmIL6hIFNG'" 1) 
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COMMENT                          1) 
COMMENT                     #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
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COMMENT                          "Creation Date: ? (no data)" 1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 10/23/07 05:38" 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5856 bp" 1) 
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COMMENT                          "Modification Date in the Original DB: 19-MAR-1980" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                          "Misc. Feature (1 total)") 
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COMMENT                          "Promoter Eukaryotic (1 total)") 
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COMMENT                                 "pMB1 ori"))) "Replication Origin (1 total)") 
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FEATURES             Location/Qualifiers
     promoter        42..519
                     /vntifkey="29"
                     /label=mIL6\promoter
                     /note="mouse interleukin 6 promoter"
     CDS             600..1100
                     /vntifkey="4"
                     /label=hIFNG
                     /note="human interferon gamma coding sequence"
     CDS             1429..1503
                     /vntifkey="4"
                     /label=part\of\SV40\small\t-antigen
                     /note="part of the SV40 small t-antigen"
     intron          1504..1570
                     /vntifkey="15"
                     /label=intron\of\SV40\small\t-antigen
                     /note="intron of the SV40 small t-antigen"
     misc_feature    1571..2122
                     /vntifkey="21"
                     /label=3'\UTR\of\SV40\small\t-antigen
                     /note="3' UTR of the SV40 small t-antigen"
     terminator      2123..2219
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     terminator      complement(2123..2219)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     rep_origin      complement(3547..3949)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     CDS             complement(4490..5350)
                     /vntifkey="4"
                     /label=ampicillin
                     /note="antibiotic resistance gene; coding sequence"
     terminator      5482..5556
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     terminator      complement(5482..5556)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     terminator      5697..5793
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     terminator      complement(5697..5793)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
BASE COUNT     1534 a      1373 c      1328 g      1621 t 
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatccttac agacatacaa aagaatccta 
       61 gcctcttatt catgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgta 
      121 tgtgtgtgtc gtctgtcatg cgcgcgtgcc tgcgtttaaa taacatcagc tttacgttct 
      181 ctttctcctt ataaaacatt gtgaatttca gttttctttc ccatcaagac atgctcaagt 
      241 gctgagtcac ttttaaagaa aaaaagaaga gtgctcatgc ttcttagggc tagcctcaag 
      301 gatgacttaa gcacactttt cccttcctag ttgtgattct ttcgatgcta aacgacgtca 
      361 cattgtgcaa tcttaataag gtttccaatc agccccaccc actctggccc cacccccacc 
      421 ctccaacaaa gatttttatc aaatgtggga ttttcccatg agtctcaaaa ttagagagtt 
      481 gactcctaat aaatatgaga ctggggatgt ctgtagctca ttctgctctg ggatctcgag 
      541 gtcagcttga tacaagaact actgatttca acttctttgg cttaattctc tcggaaacga 
      601 tgaaatatac aagttatatc ttggcttttc agctctgcat cgttttgggt tctcttggct 
      661 gttactgcca ggacccatat gtaaaagaag cagaaaacct taagaaatat tttaatgcag 
      721 gtcattcaga tgtagcggat aatggaactc ttttcttagg cattttgaag aattggaaag 
      781 aggagagtga cagaaaaata atgcagagcc aaattgtctc cttttacttc aaacttttta 
      841 aaaactttaa agatgaccag agcatccaaa agagtgtgga gaccatcaag gaagacatga 
      901 atgtcaagtt tttcaatagc aacaaaaaga aacgagatga cttcgaaaag ctgactaatt 
      961 attcggtaac tgacttgaat gtccaacgca aagcaataca tgaactcatc caagtgatgg 
     1021 ctgaactgtc gccagcagct aaaacaggga agcgaaaaag gagtcagatg ctgtttcgag 
     1081 gtcgaagagc atcccagtaa tggttgtcct gcctgcaata tttgaatttt aaatctaaat 
     1141 ctatttatta atatttaaca ttatttatat ggggaatata tttttagact catcaatcaa 
     1201 ataagtattt ataatagcaa cttttgtgta atgaaaatga atatctatta atatatgtat 
     1261 tatttataat tcctatatcc tgtgactgtc tcacttaatc ctttgttttc tgactaatta 
     1321 ggcaaggcta tgtgattaca aggctttatc tcaggggcca actaggcagc caacctaagc 
     1381 aagacctagg cttttgcaaa aagctcaagc ttggctctag agtcgacgga tctttgtgaa 
     1441 ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc 
     1501 taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg 
     1561 tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa 
     1621 tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga 
     1681 ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc 
     1741 ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt 
     1801 tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata 
     1861 ttctgtaacc tttataagta ggcataacag ttataatcat aacatactgt tttttcttac 
     1921 tccacacagg catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag 
     1981 ctttttaatt tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga 
     2041 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc 
     2101 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag 
     2161 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt 
     2221 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc 
     2281 tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct 
     2341 atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt 
     2401 gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct 
     2461 tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct 
     2521 tcctaatgca ggagtcgcat aagggagagc gtcgaccgat gcccttgaga gccttcaacc 
     2581 cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct 
     2641 tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg 
     2701 accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc 
     2761 acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg 
     2821 ccattatcgc cggcatggcg gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc 
     2881 gaggctggat ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg 
     2941 cgttgcaggc catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggat 
     3001 cgctcgcggc tcttaccagc ctaacttcga tcactggacc gctgatcgtc acggcgattt 
     3061 atgccgcctc ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc gccctatacc 
     3121 ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg ggccacctcg acctgaatgg 
     3181 aagccggcgg cacctcgcta acggattcac cactccaaga attggagcca atcaattctt 
     3241 gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac atatccatcg cgtccgccat 
     3301 ctccagcagc cgcacgcggc gcatctcggg cagcgttggg tcctggccac gggtgcgcat 
     3361 gatcgtgctc ctgtcgttga ggacccggct aggctggcgg ggttgcctta ctggttagca 
     3421 gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc tgctgcaaaa cgtctgcgac 
     3481 ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa 
     3541 gtcagcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 
     3601 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 
     3661 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 
     3721 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 
     3781 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 
     3841 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 
     3901 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 
     3961 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 
     4021 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 
     4081 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 
     4141 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga 
     4201 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 
     4261 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 
     4321 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 
     4381 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 
     4441 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 
     4501 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 
     4561 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 
     4621 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 
     4681 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 
     4741 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 
     4801 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 
     4861 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 
     4921 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 
     4981 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 
     5041 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 
     5101 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 
     5161 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 
     5221 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 
     5281 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 
     5341 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 
     5401 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 
     5461 ttccccgaaa agtgccacct gaacttgttt attgcagctt ataatggtta caaataaagc 
     5521 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 
     5581 tccaaactca tcaatgtatc ttatcatgtc tggatcataa tcagccatac cacatttgta 
     5641 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg 
     5701 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat 
     5761 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 
     5821 aaactcatca atgtatctta tcatgtctgg atctcg 
//
