ID pAS2A20 standard; circular DNA; ; 10805 BP. DT 19-MAR-1980 DE LMBP 3766. CC Accession number: LMBP 3766 CC (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium) CC Last modified: 28-OCT-2019 CC CC This file is created by Vector NTI CC http://www.invitrogen.com/ CC ORIGDB|GenBank CC VNTDBDATE|-13054552| CC LSOWNER| CC VNTAUTHORNAME|Demo User| CC VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTOAUTHORTEL|0032-9-33-13-843| CC VNTOAUTHOREML|bccm.genecorner@UGent.be| CC VNTOAUTHORWWW|www.genecorner.ugent.be| CC VNTOAUTHORAD1|Ghent University| CC VNTOAUTHORAD2|Technologiepark 927| CC VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT rep_origin complement(2..982) FT /vntifkey="33" FT /label=2mu\ori FT /note="2µ origin, incl. region conferring stability (STB)" FT CDS 1884..2558 FT /vntifkey="4" FT /label=TRP1 FT /note="S. cerevisiae tryptophan 1 coding sequence" FT rep_origin complement(2661..3116) FT /vntifkey="33" FT /label=f1\ori FT /note="phage f1 origin" FT CDS complement(3855..4304) FT /vntifkey="4" FT /label=CYH2 FT /note="CYH2 sequence encoding ribosomal protein L29" FT promoter 5075..5478 FT /vntifkey="29" FT /label=ADH1\promoter FT /note="S. cerevisiae alcohol dehydrogenase promoter" FT CDS 5501..5941 FT /vntifkey="4" FT /label=GALbd FT /note="S. cerevisiae GAL4 DNA-binding domain" FT misc_marker 5957..5983 FT /vntifkey="22" FT /label=HA\epitope FT /note="Influenza HA epitope" FT CDS 5999..8371 FT /vntifkey="4" FT /label=hA20 FT /note="human A20 coding sequence" FT terminator 8443..8544 FT /vntifkey="43" FT /label=ADH1\terminator FT /note="S. cerevisiae alcohol dehydrogenase terminator" FT rep_origin complement(8872..9274) FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT CDS complement(9815..10675) FT /vntifkey="4" FT /label=ampicillin\resistance FT /note="antibiotic resistance gene; coding sequence" FT terminator complement(1060..1164) FT /vntifkey="43" FT /label=FLP\terminator FT promoter complement(8697..8780) FT /vntifkey="30" FT /label=lac\promoter FT terminator complement(3312..3586) FT /vntifkey="43" FT /label=CYC1\terminator SQ Sequence 10805 BP; 2997 A; 2412 C; 2476 G; 2920 t; gaacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt 60 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt 120 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta 180 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg 240 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag 300 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat 360 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg 420 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact 480 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga 540 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca 600 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac 660 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa 720 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag 780 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag 840 atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt 900 ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg 960 ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt 1020 tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca 1080 cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt 1140 gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc 1200 tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact 1260 accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc 1320 tatcatttcc tttgatattg gatcatatta agaaaccatt attatcatga cattaaccta 1380 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa 1440 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 1500 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta 1560 tgcggcatca gagcagattg tactgagagt gcaccataga tcaacgacat tactatatat 1620 ataatatagg aagcatttaa tagacagcat cgtaatatat gtgtactttg cagttatgac 1680 gccagatggc agtagtggaa gatattcttt attgaaaaat agcttgtcac cttacgtaca 1740 atcttgatcc ggagcttttc tttttttgcc gattaagaat taattcggtc gaaaaaagaa 1800 aaggagaggg ccaagaggga gggcattggt gactattgag cacgtgagta tacgtgatta 1860 agcacacaaa ggcagcttgg agtatgtctg ttattaattt cacaggtagt tctggtccat 1920 tggtgaaagt ttgcggcttg cagagcacag aggccgcaga atgtgctcta gattccgatg 1980 ctgacttgct gggtattata tgtgtgccca atagaaagag aacaattgac ccggttattg 2040 caaggaaaat ttcaagtctt gtaaaagcat ataaaaatag ttcaggcact ccgaaatact 2100 tggttggcgt gtttcgtaat caacctaagg aggatgtttt ggctctggtc aatgattacg 2160 gcattgatat cgtccaactg catggagatg agtcgtggca agaataccaa gagttcctcg 2220 gtttgccagt tattaaaaga ctcgtatttc caaaagactg caacatacta ctcagtgcag 2280 cttcacagaa acctcattcg tttattccct tgtttgattc agaagcaggt gggacaggtg 2340 aacttttgga ttggaactcg atttctgact gggttggaag gcaagagagc cccgaaagct 2400 tacattttat gttagctggt ggactgacgc cagaaaatgt tggtgatgcg cttagattaa 2460 atggcgttat tggtgttgat gtaagcggag gtgtggagac aaatggtgta aaagactcta 2520 acaaaatagc aaatttcgtc aaaaatgcta agaaataggt tattactgag tagtatttat 2580 ttaagtattg tttgtgcact tgccgatcta tgcggtgtga aataccgcac agatgcgtaa 2640 ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa 2700 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa 2760 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact 2820 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc 2880 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa 2940 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc 3000 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt 3060 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcgcg 3120 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 3180 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 3240 gttttcccag tcacgacgtt gtaaaacgac ggccagtcgt ccaagctttc gcgagctcga 3300 gatcccgagc tttgcaaatt aaagccttcg agcgtcccaa aaccttctca agcaaggttt 3360 tcagtataat gttacatgcg tacacgcgtc tgtacagaaa aaaaagaaaa atttgaaata 3420 taaataacgt tcttaatact aacataacta taaaaaaata aatagggacc tagacttcag 3480 gttgtctaac tccttccttt tcggttagag cggatgtggg gggagggcgt gaatgtaagc 3540 gtgacataac taattacatg atatcgacaa aggaaaaggg gcctgtttac tcacaggctt 3600 ttttcaagta ggtaattaag tcgtttctgt ctttttcctt cttcaaccca ccaaaggcca 3660 tcttggtact tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt 3720 tttttttttt tttttttttt tttttttttt tttttttttt tttcatagaa ataatacaga 3780 agtagatgtt gaattagatt aaactgaaga tatataattt attggaaaat acatagagct 3840 ttttgttgat gcgcttaagc gatcaattca acaacaccac cagcagctct gattttttct 3900 tcagccaact tggagacgaa tctagctttg acgataactg gaacatttgg aattctaccc 3960 ttacccaaga tcttaccgta accggctgcc aaagtgtcaa taactggagc agtttcctta 4020 gaagcagatt tcaagtattg gtctctcttg tcttctggga tcaatgtcca caatttgtcc 4080 aagttcaaga ctggcttcca gaaatgagct tgttgcttgt ggaagtatct cataccaacc 4140 ttaccgaaat aacctggatg gtatttatcc atgttaattc tgtggtgatg ttgaccaccg 4200 gccatacctc taccaccggg gtgctttctg tgcttaccga tacgaccttt accggctgag 4260 acgtgacctc tgtgctttct agtcttagtg aatctggaag gcattcttga ttagttggat 4320 gattgttctg ggatttaatg caaaaaaatc actaagaagg aaaaaaatca acggagaaag 4380 caaacgccat cttaaatata cgggatacag atgaaaggtt tgaacctatc tgggaaaata 4440 cgcattaaac aagcgaaaaa ctgcgaggaa aattgtttgc gtctctgcgg gctattcacg 4500 cgccagagga aaataggaaa aataacaggg cattagaaaa ataattttga ttttggtaat 4560 gtgtgggtcc ctggtgtaca gatgttacat tggttacagt actcttgttt ttgctgtgtt 4620 tttcgatgaa tctccaaaat ggttgttagc acatggaaga gtcaccgatg ctaagttatc 4680 tctatgtaag ctacgtggcg tgacttttga tgaagccgca caagagatac aggattggca 4740 actgcaaata gaatctgggg atctagatat ccttttgttg tttccgggtg tacaatatgg 4800 acttcctctt ttctggcaac caaacccata catcgggatt cctataatac cttcgttggt 4860 ctccctaaca tgtaggtggc ggaggggaga tatacaatag aacagatacc agacaagaca 4920 taatgggcta aacaagacta caccaattac actgcctcat tgatggtggt acataacgaa 4980 ctaatactgt agccctagac ttgatagcca tcatcatatc gaagtttcac tacccttttt 5040 ccatttgcca tctattgaag taataatagg cgcatgcaac ttcttttctt tttttttctt 5100 ttctctctcc cccgttgttg tctcaccata tccgcaatga caaaaaaaat gatggaagac 5160 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga 5220 tgaggggtat cttcgaacac acgaaacttt ttccttcctt cattcacgca cactactctc 5280 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaagttt 5340 gccgctttgc tatcaagtat aaatagacct gcaattatta atcttttgtt tcctcgtcat 5400 tgttctcgtt ccctttcttc cttgtttctt tttctgcaca atatttcaag ctataccaag 5460 catacaatca actccaagct tgaagcaagc ctcctgaaag atgaagctac tgtcttctat 5520 cgaacaagca tgcgatattt gccgacttaa aaagctcaag tgctccaaag aaaaaccgaa 5580 gtgcgccaag tgtctgaaga acaactggga gtgtcgctac tctcccaaaa ccaaaaggtc 5640 tccgctgact agggcacatc tgacagaagt ggaatcaagg ctagaaagac tggaacagct 5700 atttctactg atttttcctc gagaagacct tgacatgatt ttgaaaatgg attctttaca 5760 ggatataaaa gcattgttaa caggattatt tgtacaagat aatgtgaata aagatgccgt 5820 cacagataga ttggcttcag tggagactga tatgcctcta acattgagac agcatagaat 5880 aagtgcgaca tcatcatcgg aagagagtag taacaaaggt caaagacagt tgactgtatc 5940 gccggaattc atggcttacc catacgatgt tccagattac gctagcttgg gtggtcatat 6000 ggctgaacaa gtccttcctc aggctttgta tttgagcaat atgcggaaag ctgtgaagat 6060 acgggagaga actccagaag acatttttaa acctactaat gggatcattc atcattttaa 6120 aaccatgcac cgatacacac tggaaatgtt cagaacttgc cagttttgtc ctcagtttcg 6180 ggagatcatc cacaaagccc tcatcgacag aaacatccag gccaccctgg aaagccagaa 6240 gaaactcaac tggtgtcgag aagtccggaa gcttgtggcg ctgaaaacga acggtgacgg 6300 caattgcctc atgcatgcca cttctcagta catgtggggc gttcaggaca cagacttggt 6360 actgaggaag gcgctgttca gcacgctcaa ggaaacagac acacgcaact ttaaattccg 6420 ctggcaactg gagtctctca aatctcagga atttgttgaa acggggcttt gctatgatac 6480 tcggaactgg aatgatgaat gggacaatct tatcaaaatg gcttccacag acacacccat 6540 ggcccgaagt ggacttcagt acaactcact ggaagaaata cacatatttg tcctttgcaa 6600 catcctcaga aggccaatca ttgtcatttc agacaaaatg ctaagaagtt tggaatcagg 6660 ttccaatttc gcccctttga aagtgggtgg aatttacttg cctctccact ggcctgccca 6720 ggaatgctac agatacccca ttgttctcgg ctatgacagc catcattttg tacccttggt 6780 gaccctgaag gacagtgggc ctgaaatccg agctgttcca cttgttaaca gagaccgggg 6840 aagatttgaa gacttaaaag ttcacttttt gacagatcct gaaaatgaga tgaaggagaa 6900 gctcttaaaa gagtacttaa tggtgataga aatccccgtc caaggctggg accatggcac 6960 aactcatctc atcaatgccg caaagttgga tgaagctaac ttaccaaaag aaatcaatct 7020 ggtagatgat tactttgaac ttgttcagca tgagtacaag aaatggcagg aaaacagcga 7080 gcaggggagg agagaggggc acgcccagaa tcccatggaa ccttccgtgc cccagctttc 7140 tctcatggat gtaaaatgtg aaacgcccaa ctgccccttc ttcatgtctg tgaacaccca 7200 gcctttatgc catgagtgct cagagaggcg gcaaaagaat caaaacaaac tcccaaagct 7260 gaactccaag ccgggccctg aggggctccc tggcatggcg ctcggggcct ctcggggaga 7320 agcctatgag cccttggcgt ggaaccctga ggagtccact ggggggcctc attcggcccc 7380 accgacagca cccagccctt ttctgttcag tgagaccact gccatgaagt gcaggagccc 7440 cggctgcccc ttcacactga atgtgcagca caacggattt tgtgaacgtt gccacaacgc 7500 ccggcaactt cacgccagcc acgccccaga ccacacaagg cacttggatc ccgggaagtg 7560 ccaagcctgc ctccaggatg ttaccaggac atttaatggg atctgcagta cttgcttcaa 7620 aaggactaca gcagaggcct cctccagcct cagcaccagc ctccctcctt cctgtcacca 7680 gcgttccaag tcagatccct cgcggctcgt ccggagcccc tccccgcatt cttgccacag 7740 agctggaaac gacgcccctg ctggctgcct gtctcaagct gcacggactc ctggggacag 7800 gacggggacg agcaagtgca gaaaagccgg ctgcgtgtat tttgggactc cagaaaacaa 7860 gggcttttgc acactgtgtt tcatcgagta cagagaaaac aaacattttg ctgctgcctc 7920 agggaaagtc agtcccacag cgtccaggtt ccagaacacc attccgtgcc tggggaggga 7980 atgcggcacc cttggaagca ccatgtttga aggatactgc cagaagtgtt tcattgaagc 8040 tcagaatcag agatttcatg aggccaaaag gacagaagag caactgagat cgagccagcg 8100 cagagatgtg cctcgaacca cacaaagcac ctcaaggccc aagtgcgccc gggcctcctg 8160 caagaacatc ctggcctgcc gcagcgagga gctctgcatg gagtgtcagc atcccaacca 8220 gaggatgggc cctggggccc accggggtga gcctgccccc gaagaccccc ccaagcagcg 8280 ttgccgggcc cccgcctgtg atcattttgg caatgccaag tgcaacggct actgcaacga 8340 atgctttcag ttcaagcaga tgtatggcta accggaaaca ggtgggtcac ctcctgcaag 8400 aagtggggcc tcgaggtcga cctgcagcca agctaattcc gggcgaattt cttatgattt 8460 atgattttta ttattaaata agttataaaa aaaataagtg tatacaaatt ttaaagtgac 8520 tcttaggttt taaaacgaaa attcttgttc ttgagtaact ctttcctgta ggtcaggttg 8580 ctttctcagg tatagcatga ggtcgctctt attgaccaca cctctaccgg catgcaagct 8640 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 8700 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgaggtaac 8760 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc 8820 tggattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg 8880 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 8940 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 9000 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 9060 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 9120 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 9180 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 9240 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 9300 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 9360 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 9420 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 9480 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 9540 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 9600 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 9660 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 9720 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 9780 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 9840 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 9900 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 9960 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 10020 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 10080 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 10140 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 10200 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 10260 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 10320 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 10380 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 10440 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 10500 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 10560 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 10620 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 10680 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 10740 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 10800 cacct 10805 //