LOCUS       pAS2hA20S565A          10805 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 3772.
SOURCE      
  ORGANISM  
COMMENT     VNTNAME|pAS2hA20S565A|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pAS2hA20S565A" 0 10805 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 10805 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
COMMENT        #3=(CFSignal (CObList) "2MU-O" 33 0 1 2 982 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2..982)") 
COMMENT        #4=(CFSignal (CObList) "TRP1" 4 0 0 1884 2558 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1884..2558") 
COMMENT        #5=(CFSignal (CObList) "f1ORI" 33 0 1 2661 3116 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2661..3116)") 
COMMENT        #6=(CFSignal (CObList) "CYH2" 4 0 1 3855 4304 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(3855..4304)") 
COMMENT        #7=(CFSignal (CObList) "ADH1" 30 0 0 5075 5478 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "5075..5478") 
COMMENT        #8=(CFSignal (CObList) "GALbd" 4 0 0 5501 5941 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "5501..5941") 
COMMENT        #9=(CFSignal (CObList) "HA" 4 0 0 5957 5983 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "5957..5983") 
COMMENT        #10=(CFSignal (CObList) "hA20m" 4 0 0 5999 8371 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5999..8371") 
COMMENT        #11=(CFSignal (CObList) "ADH1T" 43 0 0 8443 8544 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "8443..8544") 
COMMENT        #12=(CFSignal (CObList) "ORI" 33 0 1 8872 9274 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(8872..9274)") 
COMMENT        #13=(CFSignal (CObList) "AMP" 4 0 1 9815 10675 0 (CStringList) 
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COMMENT                          "DNA 'pAS2hA20S565A'" 1) 
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COMMENT                          "Foreign object. Author: UNKNOWN. Original author: BCCM/GeneCorner Plasmid Collection" 
COMMENT                          1) 
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COMMENT                          "Creation Date: ? (no data)" 1) 
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COMMENT                          "Last Modification Date: ? (no data)" 1) 
COMMENT                     #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 10805 bp" 1) 
COMMENT                     #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "Original Source Database: GenBank" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                                        "Start: 1884  End: 2558" 1) 
COMMENT                                   #32=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "S. cerevisiae tryptophan 1 coding sequence" 
COMMENT                                        1) 
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COMMENT                                        "Original Location Description:" 1) 
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COMMENT                                        "   1884..2558" 1))) "TRP1") 
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COMMENT                                        "Start: 3855  End: 4304 (Complementary)" 
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COMMENT                                   #37=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "CYH2 sequence encoding ribosomal protein L29" 
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COMMENT                                        "Start: 5501  End: 5941" 1) 
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COMMENT                                        "S. cerevisiae GAL4 DNA-binding domain" 
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COMMENT                                        "Start: 5957  End: 5983" 1) 
COMMENT                                   #47=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Influenza HA epitope" 1) 
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COMMENT                                        "Start: 5999  End: 8371" 1) 
COMMENT                                   #52=(CLinePar 
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COMMENT                                        "mutated human A20 coding sequence" 1) 
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COMMENT                                        "Start: 9815  End: 10675 (Complementary)" 
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COMMENT                                        "antibiotic resistance gene; coding sequence" 
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COMMENT                          "Replication Origin (3 total)") 
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COMMENT                          "Terminator (1 total)"))) "Feature Map")))) 
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FEATURES             Location/Qualifiers
     rep_origin      complement(2..982)
                     /vntifkey="33"
                     /label=2MU-O
                     /note="2ò origin, incl. region conferring stability (STB)"
     CDS             1884..2558
                     /vntifkey="4"
                     /label=TRP1
                     /note="S. cerevisiae tryptophan 1 coding sequence"
     rep_origin      complement(2661..3116)
                     /vntifkey="33"
                     /label=f1ORI
                     /note="phage f1 origin"
     CDS             complement(3855..4304)
                     /vntifkey="4"
                     /label=CYH2
                     /note="CYH2 sequence encoding ribosomal protein L29"
     promoter        5075..5478
                     /vntifkey="30"
                     /label=ADH1
                     /note="S. cerevisiae alcohol dehydrogenase promoter"
     CDS             5501..5941
                     /vntifkey="4"
                     /label=GALbd
                     /note="S. cerevisiae GAL4 DNA-binding domain"
     CDS             5957..5983
                     /vntifkey="4"
                     /label=HA
                     /note="Influenza HA epitope"
     CDS             5999..8371
                     /vntifkey="4"
                     /label=hA20m
                     /note="mutated human A20 coding sequence"
     terminator      8443..8544
                     /vntifkey="43"
                     /label=ADH1T
                     /note="S. cerevisiae alcohol dehydrogenase terminator"
     rep_origin      complement(8872..9274)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     CDS             complement(9815..10675)
                     /vntifkey="4"
                     /label=AMP
                     /note="antibiotic resistance gene; coding sequence"
BASE COUNT     2998 a      2411 c      2478 g      2918 t 
ORIGIN
        1 gaacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt 
       61 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt 
      121 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta 
      181 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg 
      241 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag 
      301 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat 
      361 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg 
      421 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact 
      481 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga 
      541 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca 
      601 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac 
      661 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa 
      721 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag 
      781 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag 
      841 atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt 
      901 ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg 
      961 ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt 
     1021 tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca 
     1081 cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt 
     1141 gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc 
     1201 tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact 
     1261 accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc 
     1321 tatcatttcc tttgatattg gatcatatta agaaaccatt attatcatga cattaaccta 
     1381 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa 
     1441 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 
     1501 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta 
     1561 tgcggcatca gagcagattg tactgagagt gcaccataga tcaacgacat tactatatat 
     1621 ataatatagg aagcatttaa tagacagcat cgtaatatat gtgtactttg cagttatgac 
     1681 gccagatggc agtagtggaa gatattcttt attgaaaaat agcttgtcac cttacgtaca 
     1741 atcttgatcc ggagcttttc tttttttgcc gattaagaat taattcggtc gaaaaaagaa 
     1801 aaggagaggg ccaagaggga gggcattggt gactattgag cacgtgagta tacgtgatta 
     1861 agcacacaaa ggcagcttgg agtatgtctg ttattaattt cacaggtagt tctggtccat 
     1921 tggtgaaagt ttgcggcttg cagagcacag aggccgcaga atgtgctcta gattccgatg 
     1981 ctgacttgct gggtattata tgtgtgccca atagaaagag aacaattgac ccggttattg 
     2041 caaggaaaat ttcaagtctt gtaaaagcat ataaaaatag ttcaggcact ccgaaatact 
     2101 tggttggcgt gtttcgtaat caacctaagg aggatgtttt ggctctggtc aatgattacg 
     2161 gcattgatat cgtccaactg catggagatg agtcgtggca agaataccaa gagttcctcg 
     2221 gtttgccagt tattaaaaga ctcgtatttc caaaagactg caacatacta ctcagtgcag 
     2281 cttcacagaa acctcattcg tttattccct tgtttgattc agaagcaggt gggacaggtg 
     2341 aacttttgga ttggaactcg atttctgact gggttggaag gcaagagagc cccgaaagct 
     2401 tacattttat gttagctggt ggactgacgc cagaaaatgt tggtgatgcg cttagattaa 
     2461 atggcgttat tggtgttgat gtaagcggag gtgtggagac aaatggtgta aaagactcta 
     2521 acaaaatagc aaatttcgtc aaaaatgcta agaaataggt tattactgag tagtatttat 
     2581 ttaagtattg tttgtgcact tgccgatcta tgcggtgtga aataccgcac agatgcgtaa 
     2641 ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa 
     2701 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa 
     2761 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact 
     2821 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc 
     2881 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa 
     2941 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc 
     3001 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt 
     3061 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcgcg 
     3121 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 
     3181 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 
     3241 gttttcccag tcacgacgtt gtaaaacgac ggccagtcgt ccaagctttc gcgagctcga 
     3301 gatcccgagc tttgcaaatt aaagccttcg agcgtcccaa aaccttctca agcaaggttt 
     3361 tcagtataat gttacatgcg tacacgcgtc tgtacagaaa aaaaagaaaa atttgaaata 
     3421 taaataacgt tcttaatact aacataacta taaaaaaata aatagggacc tagacttcag 
     3481 gttgtctaac tccttccttt tcggttagag cggatgtggg gggagggcgt gaatgtaagc 
     3541 gtgacataac taattacatg atatcgacaa aggaaaaggg gcctgtttac tcacaggctt 
     3601 ttttcaagta ggtaattaag tcgtttctgt ctttttcctt cttcaaccca ccaaaggcca 
     3661 tcttggtact tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt 
     3721 tttttttttt tttttttttt tttttttttt tttttttttt tttcatagaa ataatacaga 
     3781 agtagatgtt gaattagatt aaactgaaga tatataattt attggaaaat acatagagct 
     3841 ttttgttgat gcgcttaagc gatcaattca acaacaccac cagcagctct gattttttct 
     3901 tcagccaact tggagacgaa tctagctttg acgataactg gaacatttgg aattctaccc 
     3961 ttacccaaga tcttaccgta accggctgcc aaagtgtcaa taactggagc agtttcctta 
     4021 gaagcagatt tcaagtattg gtctctcttg tcttctggga tcaatgtcca caatttgtcc 
     4081 aagttcaaga ctggcttcca gaaatgagct tgttgcttgt ggaagtatct cataccaacc 
     4141 ttaccgaaat aacctggatg gtatttatcc atgttaattc tgtggtgatg ttgaccaccg 
     4201 gccatacctc taccaccggg gtgctttctg tgcttaccga tacgaccttt accggctgag 
     4261 acgtgacctc tgtgctttct agtcttagtg aatctggaag gcattcttga ttagttggat 
     4321 gattgttctg ggatttaatg caaaaaaatc actaagaagg aaaaaaatca acggagaaag 
     4381 caaacgccat cttaaatata cgggatacag atgaaaggtt tgaacctatc tgggaaaata 
     4441 cgcattaaac aagcgaaaaa ctgcgaggaa aattgtttgc gtctctgcgg gctattcacg 
     4501 cgccagagga aaataggaaa aataacaggg cattagaaaa ataattttga ttttggtaat 
     4561 gtgtgggtcc ctggtgtaca gatgttacat tggttacagt actcttgttt ttgctgtgtt 
     4621 tttcgatgaa tctccaaaat ggttgttagc acatggaaga gtcaccgatg ctaagttatc 
     4681 tctatgtaag ctacgtggcg tgacttttga tgaagccgca caagagatac aggattggca 
     4741 actgcaaata gaatctgggg atctagatat ccttttgttg tttccgggtg tacaatatgg 
     4801 acttcctctt ttctggcaac caaacccata catcgggatt cctataatac cttcgttggt 
     4861 ctccctaaca tgtaggtggc ggaggggaga tatacaatag aacagatacc agacaagaca 
     4921 taatgggcta aacaagacta caccaattac actgcctcat tgatggtggt acataacgaa 
     4981 ctaatactgt agccctagac ttgatagcca tcatcatatc gaagtttcac tacccttttt 
     5041 ccatttgcca tctattgaag taataatagg cgcatgcaac ttcttttctt tttttttctt 
     5101 ttctctctcc cccgttgttg tctcaccata tccgcaatga caaaaaaaat gatggaagac 
     5161 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga 
     5221 tgaggggtat cttcgaacac acgaaacttt ttccttcctt cattcacgca cactactctc 
     5281 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaagttt 
     5341 gccgctttgc tatcaagtat aaatagacct gcaattatta atcttttgtt tcctcgtcat 
     5401 tgttctcgtt ccctttcttc cttgtttctt tttctgcaca atatttcaag ctataccaag 
     5461 catacaatca actccaagct tgaagcaagc ctcctgaaag atgaagctac tgtcttctat 
     5521 cgaacaagca tgcgatattt gccgacttaa aaagctcaag tgctccaaag aaaaaccgaa 
     5581 gtgcgccaag tgtctgaaga acaactggga gtgtcgctac tctcccaaaa ccaaaaggtc 
     5641 tccgctgact agggcacatc tgacagaagt ggaatcaagg ctagaaagac tggaacagct 
     5701 atttctactg atttttcctc gagaagacct tgacatgatt ttgaaaatgg attctttaca 
     5761 ggatataaaa gcattgttaa caggattatt tgtacaagat aatgtgaata aagatgccgt 
     5821 cacagataga ttggcttcag tggagactga tatgcctcta acattgagac agcatagaat 
     5881 aagtgcgaca tcatcatcgg aagagagtag taacaaaggt caaagacagt tgactgtatc 
     5941 gccggaattc atggcttacc catacgatgt tccagattac gctagcttgg gtggtcatat 
     6001 ggctgaacaa gtccttcctc aggctttgta tttgagcaat atgcggaaag ctgtgaagat 
     6061 acgggagaga actccagaag acatttttaa acctactaat gggatcattc atcattttaa 
     6121 aaccatgcac cgatacacac tggaaatgtt cagaacttgc cagttttgtc ctcagtttcg 
     6181 ggagatcatc cacaaagccc tcatcgacag aaacatccag gccaccctgg aaagccagaa 
     6241 gaaactcaac tggtgtcgag aagtccggaa gcttgtggcg ctgaaaacga acggtgacgg 
     6301 caattgcctc atgcatgcca cttctcagta catgtggggc gttcaggaca cagacttggt 
     6361 actgaggaag gcgctgttca gcacgctcaa ggaaacagac acacgcaact ttaaattccg 
     6421 ctggcaactg gagtctctca aatctcagga atttgttgaa acggggcttt gctatgatac 
     6481 tcggaactgg aatgatgaat gggacaatct tatcaaaatg gcttccacag acacacccat 
     6541 ggcccgaagt ggacttcagt acaactcact ggaagaaata cacatatttg tcctttgcaa 
     6601 catcctcaga aggccaatca ttgtcatttc agacaaaatg ctaagaagtt tggaatcagg 
     6661 ttccaatttc gcccctttga aagtgggtgg aatttacttg cctctccact ggcctgccca 
     6721 ggaatgctac agatacccca ttgttctcgg ctatgacagc catcattttg tacccttggt 
     6781 gaccctgaag gacagtgggc ctgaaatccg agctgttcca cttgttaaca gagaccgggg 
     6841 aagatttgaa gacttaaaag ttcacttttt gacagatcct gaaaatgaga tgaaggagaa 
     6901 gctcttaaaa gagtacttaa tggtgataga aatccccgtc caaggctggg accatggcac 
     6961 aactcatctc atcaatgccg caaagttgga tgaagctaac ttaccaaaag aaatcaatct 
     7021 ggtagatgat tactttgaac ttgttcagca tgagtacaag aaatggcagg aaaacagcga 
     7081 gcaggggagg agagaggggc acgcccagaa tcccatggaa ccttccgtgc cccagctttc 
     7141 tctcatggat gtaaaatgtg aaacgcccaa ctgccccttc ttcatgtctg tgaacaccca 
     7201 gcctttatgc catgagtgct cagagaggcg gcaaaagaat caaaacaaac tcccaaagct 
     7261 gaactccaag ccgggccctg aggggctccc tggcatggcg ctcggggcct ctcggggaga 
     7321 agcctatgag cccttggcgt ggaaccctga ggagtccact ggggggcctc attcggcccc 
     7381 accgacagca cccagccctt ttctgttcag tgagaccact gccatgaagt gcaggagccc 
     7441 cggctgcccc ttcacactga atgtgcagca caacggattt tgtgaacgtt gccacaacgc 
     7501 ccggcaactt cacgccagcc acgccccaga ccacacaagg cacttggatc ccgggaagtg 
     7561 ccaagcctgc ctccaggatg ttaccaggac atttaatggg atctgcagta cttgcttcaa 
     7621 aaggactaca gcagaggcct cctccagcct cagcaccagc ctccctcctt cctgtcacca 
     7681 gcgatcgaag gcagatccct cgcggctcgt ccggagcccc tccccgcatt cttgccacag 
     7741 agctggaaac gacgcccctg ctggctgcct gtctcaagct gcacggactc ctggggacag 
     7801 gacggggacg agcaagtgca gaaaagccgg ctgcgtgtat tttgggactc cagaaaacaa 
     7861 gggcttttgc acactgtgtt tcatcgagta cagagaaaac aaacattttg ctgctgcctc 
     7921 agggaaagtc agtcccacag cgtccaggtt ccagaacacc attccgtgcc tggggaggga 
     7981 atgcggcacc cttggaagca ccatgtttga aggatactgc cagaagtgtt tcattgaagc 
     8041 tcagaatcag agatttcatg aggccaaaag gacagaagag caactgagat cgagccagcg 
     8101 cagagatgtg cctcgaacca cacaaagcac ctcaaggccc aagtgcgccc gggcctcctg 
     8161 caagaacatc ctggcctgcc gcagcgagga gctctgcatg gagtgtcagc atcccaacca 
     8221 gaggatgggc cctggggccc accggggtga gcctgccccc gaagaccccc ccaagcagcg 
     8281 ttgccgggcc cccgcctgtg atcattttgg caatgccaag tgcaacggct actgcaacga 
     8341 atgctttcag ttcaagcaga tgtatggcta accggaaaca ggtgggtcac ctcctgcaag 
     8401 aagtggggcc tcgaggtcga cctgcagcca agctaattcc gggcgaattt cttatgattt 
     8461 atgattttta ttattaaata agttataaaa aaaataagtg tatacaaatt ttaaagtgac 
     8521 tcttaggttt taaaacgaaa attcttgttc ttgagtaact ctttcctgta ggtcaggttg 
     8581 ctttctcagg tatagcatga ggtcgctctt attgaccaca cctctaccgg catgcaagct 
     8641 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 
     8701 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgaggtaac 
     8761 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc 
     8821 tggattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg 
     8881 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 
     8941 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 
     9001 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 
     9061 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 
     9121 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 
     9181 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 
     9241 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 
     9301 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 
     9361 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 
     9421 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 
     9481 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 
     9541 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 
     9601 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 
     9661 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 
     9721 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 
     9781 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 
     9841 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 
     9901 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 
     9961 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 
    10021 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 
    10081 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 
    10141 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 
    10201 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 
    10261 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 
    10321 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 
    10381 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 
    10441 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 
    10501 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 
    10561 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 
    10621 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 
    10681 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 
    10741 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 
    10801 cacct 
//
