LOCUS       pCAGGS-mCASP-11         6280 bp    DNA     circular      3-JUN-2008
DEFINITION  LMBP 3816.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 3816
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 3-JUN-2008
            
COMMENT     VNTDATE|484157644|
COMMENT     VNTNAME|pCAGGS-mCASP-11|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-mCASP-11" 0 6280 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "BamHI" "BglII" "HindIII" "NcoI" "XhoI" "CaiI" "Cfr42I" 
COMMENT         "Eco147I" "PdmI" "MscI" "SgrAI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 6280 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #3=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 2 2220 0 2557 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "BglII" "agatct" 2 0 2 934 0 1570 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 2 413 0 2036 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 3 358 0 2313 0 4929 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 5 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "CaiI" "cagnnnctg" 7 0 2 3154 0 6233 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "Cfr42I" "ccgcgg" 5 0 2 5585 0 6114 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "Eco147I" "aggcct" 4 0 3 585 0 863 0 2405 0 0 
COMMENT             "") 
COMMENT        #11=(CRSite (CStringList) "PdmI" "gaannnnttc" 6 0 3 318 0 989 0 4230 0 0 
COMMENT             "") #12=(CRSite (CStringList) "MscI" "tggcca" 4 0 1 1284 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "SgrAI" "crccggyg" 3 0 1 5868 0 0 "")) 
COMMENT       (CObList 
COMMENT        #14=(CFSignal (CObList) "mCASP-11" 4 0 0 106 1227 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "106..1227") 
COMMENT        #15=(CFSignal (CObList) "5' UTR of mCASP-11" 21 0 0 80 105 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "80..105") 
COMMENT        #16=(CFSignal (CObList) "intron AG promoter" 15 0 0 5305 6229 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "5307..6231") 
COMMENT        #17=(CFSignal (CObList) "AG promoter" 29 0 0 4936 6277 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4938..6279") 
COMMENT        #18=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 4553 4930 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4555..4932") 
COMMENT        #19=(CFSignal (CObList) "ampicillin resistance" 4 0 1 3557 4417 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(3559..4419)") 
COMMENT        #20=(CFSignal (CObList) "pMB1 ori" 33 0 1 2615 3016 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2617..3018)") 
COMMENT        #21=(CFSignal (CObList) "SV40 polyA late" 43 0 1 2426 2500 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(2428..2502)") 
COMMENT        #22=(CFSignal (CObList) "SV40 polyA early" 43 0 0 2426 2500 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "2428..2502") 
COMMENT        #23=(CFSignal (CObList) "SV40 ori" 33 0 0 2320 2395 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2322..2397") 
COMMENT        #24=(CFSignal (CObList) "lac promoter" 30 0 1 2096 2178 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2098..2180)") 
COMMENT        #25=(CFSignal (CObList) "3' FR of \337-globin" 21 0 0 1662 2018 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "1664..2020") 
COMMENT        #26=(CFSignal (CObList) "\337-globin polyA" 43 0 0 1570 1661 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "1572..1663") 
COMMENT        #27=(CFSignal (CObList) "3' UTR of mCASP-11" 21 0 0 1228 1420 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "")) (CObList) (CObList) 
COMMENT       (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #28=(CFSignal (CObList) "LMBP 3816" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #29=(CFSignal (CObList) "Last modified: 3-JUN-2008" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
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COMMENT                    2) 5 "" 0 4) 
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COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'pCAGGS-mCASP-11'" 1) 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 3816" 
COMMENT                          1) 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
COMMENT                          1) 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 06/03/08 04:14" 1) 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 06/03/08 04:17" 1) 
COMMENT                     #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6280 bp" 1) 
COMMENT                     #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #40=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #41=(CFolderPar 
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COMMENT                   "Standard Fields") 
COMMENT              #42=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 5 (0 0) 1 1 0 0 178) 
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COMMENT                          "BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #44=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "EMAIL: <a href=\"mailto:bccm.genecorner@UGent.be\">bccm.lmbp@UGent.be</a>" 
COMMENT                          1))) "Original Author") 
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COMMENT                     #47=(CFolderPar 
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COMMENT                           (CObjectList 
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COMMENT                                 "" 1))) "LMBP 3816") 
COMMENT                     #49=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #50=(CLinePar (CParagraph 0 (15 #29# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "Last modified: 3-JUN-2008"))) 
COMMENT                   "Annotations") 
COMMENT              #51=(CFolderPar 
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COMMENT                     #52=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
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COMMENT                            #53=(CFolderPar 
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COMMENT                                   #54=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 106   End: 1227" 1) 
COMMENT                                   #55=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "mouse caspase-11 coding sequence" 1) 
COMMENT                                   #56=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #57=(CLinePar 
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COMMENT                                        "   106..1227" 1))) "mCASP-11") 
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COMMENT                                        "Start: 3557  End: 4417 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #60=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "antibiotic resistance gene; coding sequence" 
COMMENT                                        1) 
COMMENT                                   #61=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #62=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(3559..4419)" 1))) 
COMMENT                                 "ampicillin resistance"))) "CDS (2 total)") 
COMMENT                     #63=(CFolderPar 
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COMMENT                           (CObjectList 
COMMENT                            #64=(CFolderPar 
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COMMENT                                  (CParagraph 5305 (3 #16# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #65=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5305  End: 6229" 1) 
COMMENT                                   #66=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "chicken \337-actin/rabbit \337-globin hybrid intron" 
COMMENT                                        1) 
COMMENT                                   #67=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #68=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   5307..6231" 1))) 
COMMENT                                 "intron AG promoter"))) "Intron (1 total)") 
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COMMENT                                        "Start: 80    End: 105 " 1) 
COMMENT                                   #72=(CLinePar 
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COMMENT                                        "5' UTR of the mouse caspase-11 gene" 1) 
COMMENT                                   #73=(CLinePar 
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COMMENT                                   #74=(CLinePar 
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COMMENT                                        "   80..105" 1))) "5' UTR of mCASP-11") 
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COMMENT                                   #76=(CLinePar 
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COMMENT                                        "Start: 1228  End: 1420" 1) 
COMMENT                                   #77=(CLinePar 
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COMMENT                                        "3' UTR of the mouse caspase-11 gene" 1))) 
COMMENT                                 "3' UTR of mCASP-11") 
COMMENT                            #78=(CFolderPar 
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COMMENT                                        "Start: 1662  End: 2018" 1) 
COMMENT                                   #80=(CLinePar 
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COMMENT                                        "3' flanking region of the rabbit \337-globin gene" 
COMMENT                                        1) 
COMMENT                                   #81=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1664..2020" 1))) 
COMMENT                                 "3' FR of \337-globin"))) 
COMMENT                          "Misc. Feature (3 total)") 
COMMENT                     #83=(CFolderPar 
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COMMENT                           (CObjectList 
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COMMENT                                        "Start: 4553  End: 4930" 1) 
COMMENT                                   #86=(CLinePar 
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COMMENT                                        "human CMV-IE enhancer" 1) 
COMMENT                                   #87=(CLinePar 
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COMMENT                                   #88=(CLinePar 
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COMMENT                                        "   4555..4932" 1))) "hCMV-IE enhancer") 
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COMMENT                                        "Start: 4936  End: 6277" 1) 
COMMENT                                   #91=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "chicken \337-actin/rabbit \337-globin hybrid promoter" 
COMMENT                                        1) 
COMMENT                                   #92=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
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COMMENT                                   #93=(CLinePar 
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COMMENT                                        "   4938..6279" 1))) "AG promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
COMMENT                     #94=(CFolderPar 
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COMMENT                                        "Start: 2096  End: 2178 (Complementary)" 
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COMMENT                                   #97=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "E. coli lac operon promoter" 1) 
COMMENT                                   #98=(CLinePar 
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COMMENT                                   #99=(CLinePar 
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COMMENT                                        "   complement(2098..2180)" 1))) 
COMMENT                                 "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
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COMMENT                                           "Start: 2320  End: 2395" 1) 
COMMENT                                     #103=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "bidirectional origin of SV40" 1) 
COMMENT                                     #104=(CLinePar 
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COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #105=(CLinePar 
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COMMENT                                           "   2322..2397" 1))) "SV40 ori") 
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COMMENT                                           "Start: 2615  End: 3016 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #108=(CLinePar 
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COMMENT                                           "E. coli plasmid pMB1 origin" 1) 
COMMENT                                     #109=(CLinePar 
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COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #110=(CLinePar 
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COMMENT                                           "   complement(2617..3018)" 1))) 
COMMENT                                   "pMB1 ori"))) "Replication Origin (2 total)") 
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COMMENT                                           "Start: 1570  End: 1661" 1) 
COMMENT                                     #114=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "3' UTR of the rabbit \337-globin gene, incl. polyA" 
COMMENT                                           1) 
COMMENT                                     #115=(CLinePar 
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COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #116=(CLinePar 
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COMMENT                                           "   1572..1663" 1))) 
COMMENT                                   "\337-globin polyA") 
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COMMENT                                           "Start: 2426  End: 2500 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #119=(CLinePar 
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COMMENT                                           "polyA signal of the SV40 late region" 
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FEATURES             Location/Qualifiers
     CDS             106..1227
                     /vntifkey="4"
                     /label=mCASP-11
                     /note="mouse caspase-11 coding sequence"
     misc_feature    80..105
                     /vntifkey="21"
                     /label=5'\UTR\of\mCASP-11
                     /note="5' UTR of the mouse caspase-11 gene"
     intron          5305..6229
                     /vntifkey="15"
                     /label=intron\AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid intron"
     promoter        4936..6277
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     promoter        4553..4930
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
                     /note="human CMV-IE enhancer"
     CDS             complement(3557..4417)
                     /vntifkey="4"
                     /label=ampicillin\resistance
                     /note="antibiotic resistance gene; coding sequence"
     rep_origin      complement(2615..3016)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="E. coli plasmid pMB1 origin"
     terminator      complement(2426..2500)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     terminator      2426..2500
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     rep_origin      2320..2395
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="bidirectional origin of SV40"
     promoter        complement(2096..2178)
                     /vntifkey="30"
                     /label=lac\promoter
                     /note="E. coli lac operon promoter"
     misc_feature    1662..2018
                     /vntifkey="21"
                     /label=3'\FR\of\ß-globin
                     /note="3' flanking region of the rabbit ß-globin gene"
     terminator      1570..1661
                     /vntifkey="43"
                     /label=ß-globin\polyA
                     /note="3' UTR of the rabbit ß-globin gene, incl. polyA"
     misc_feature    1228..1420
                     /vntifkey="21"
                     /label=3'\UTR\of\mCASP-11
                     /note="3' UTR of the mouse caspase-11 gene"
BASE COUNT     1483 a      1588 c      1659 g      1550 t 
ORIGIN
        1 ttcctcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg 
       61 ggggaattcg gcacgagcgg tgcgaaagaa ctgaggcttt ttctcatggc tgaaaacaaa 
      121 caccctgaca aaccacttaa ggtgttggaa cagctgggca aagaagtcct tacggagtac 
      181 ctagaaaaat tagtacaaag caatgtactg aaattaaagg aggaagataa acaaaaattt 
      241 aacaatgctg aacgcagtga caagcgttgg gtttttgtag atgccatgaa aaagaaacac 
      301 agcaaagtag gtgaaatgct tctccagaca ttcttcagtg tggacccagg cagccaccat 
      361 ggtgaagcta atctggaaat ggaggaacca gaagaatcat tgaacactct caagctttgt 
      421 tcccctgaag agttcacaag gctttgcaga gaaaagacac aagaaattta cccaataaag 
      481 gaggccaatg gccgtacacg aaaggctctt atcatatgca atacagagtt caaacatctc 
      541 tcactgaggt atggggctaa ctttgacatc attggtatga aaggccttct tgaagactta 
      601 ggctacgatg tggtggtgaa agaggagctt acagcagagg gcatggagtc agagatgaaa 
      661 gactttgctg cactctcaga acaccagaca tcagacagca cattcctggt gctaatgtct 
      721 catggcacac tgcatggcat ttgtggaaca atgcacagtg aaaaaactcc agatgtgcta 
      781 cagtatgata ccatctatca gatattcaac aattgccact gtccaggtct acgagacaaa 
      841 cccaaagtca tcattgtgca ggcctgcaga ggtgggaact ctggagaaat gtggatcaga 
      901 gagtcttcaa aaccccagtt gtgcagaggt gtagatctac ctaggaatat ggaagctgat 
      961 gctgtcaagc tgagccacgt ggagaaggac ttcattgcct tctactctac aaccccacat 
     1021 cacttgtcct accgagacaa aacaggaggc tcttacttca tcactagact catttcctgc 
     1081 ttccggaaac atgcttgctc ttgtcatctc tttgatatat tcctgaaggt gcaacaatca 
     1141 tttgaaaagg caagtattca ttcccagatg cccaccattg atcgggcaac cttgacgaga 
     1201 tatttctacc tctttcctgg caactgagaa caaagcaaca agcaactgaa tctcatttct 
     1261 tcagcttgaa gaagtgatct tggccaagga tcacattcta ttcctgaaat tccagaacta 
     1321 gtgaaattaa ggaaagaata cttatgaatt caagaccagc ctaagcaaca cagtgggatt 
     1381 ctgttccata gacaagcaaa caagcaaaaa taaaaaaaaa ctcgtgccga attcgatctc 
     1441 tttttttggg tttggtgggg tatcttcatc atcgaataga tagttatata catcatccat 
     1501 tgtagtggta ttaaacatcc ctgtagtgat tccaaacgcg ccaatgccct ggctcacaaa 
     1561 taccactgag atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag 
     1621 catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt 
     1681 ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag 
     1741 tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg 
     1801 ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga 
     1861 aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta 
     1921 acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta 
     1981 ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacctgca gcccaagctt 
     2041 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 
     2101 caacatacga gccggaagca taaagtgtaa agcctgggtg cctaatgagt gagctaactc 
     2161 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg 
     2221 atccgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc 
     2281 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 
     2341 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg 
     2401 gaggcctagg cttttgcaaa aagctaactt gtttattgca gcttataatg gttacaaata 
     2461 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 
     2521 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgctgcatta atgaatcggc 
     2581 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 
     2641 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 
     2701 cggttatcca cagaatcagg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 
     2761 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 
     2821 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 
     2881 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 
     2941 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 
     3001 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 
     3061 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 
     3121 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 
     3181 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa 
     3241 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 
     3301 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 
     3361 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 
     3421 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 
     3481 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 
     3541 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 
     3601 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 
     3661 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 
     3721 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 
     3781 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 
     3841 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt 
     3901 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 
     3961 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 
     4021 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 
     4081 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 
     4141 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca 
     4201 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 
     4261 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 
     4321 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 
     4381 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt 
     4441 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 
     4501 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctggg tcgacattga 
     4561 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg 
     4621 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc 
     4681 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat 
     4741 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat 
     4801 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat 
     4861 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc 
     4921 gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc 
     4981 tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg 
     5041 gggggggggg gggggccccc cccaggcggg gcggggcggg gcgaggggcg gggcggggcg 
     5101 aggcggaaag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg 
     5161 gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgtt 
     5221 gcgcgctgcc ttccccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc 
     5281 tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt 
     5341 aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga aagccttgag 
     5401 gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg 
     5461 tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc 
     5521 ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc 
     5581 ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg 
     5641 gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca cccccctccc 
     5701 cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg 
     5761 ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct 
     5821 cgggccgggg agggctcggg ggaaggggcg cggcggcccc cggagcgccg gcggctgtcg 
     5881 aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg cgcagggact 
     5941 tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca ccccctctag 
     6001 cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg agggccttcg 
     6061 tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc cgcgggggga 
     6121 cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg 
     6181 ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa 
     6241 cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa 
//
