LOCUS       pCAGGS-mCASP-7          5929 bp    DNA     circular     31-MAR-2008
DEFINITION  LMBP 3818.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 3818
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 31-MAR-2008
            
COMMENT     VNTNAME|pCAGGS-mCASP-7|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-mCASP-7" 0 5929 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "AflIII" "HindII" "HindIII" "PstI" "SacII" "XhoI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 5929 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #3=(CRSite (CStringList) "HindII" "gtyrac" 4 0 3 333 0 1177 0 4202 0 0 
COMMENT            "") #4=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 1685 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "PstI" "ctgcag" 6 0 2 132 0 1680 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 2 5234 0 5763 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "AflIII" "acrygt" 2 0 2 1591 0 2387 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 2 5 0 1033 0 0 "")) 
COMMENT       (CObList 
COMMENT        #9=(CFSignal (CObList) "5' UTR of mCASP-7" 21 0 0 78 266 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "78..266") 
COMMENT        #10=(CFSignal (CObList) "mCASP-7" 4 0 0 267 1178 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "267..1178") 
COMMENT        #11=(CFSignal (CObList) "3' UTR of mCASP-7" 21 0 0 1179 1189 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "1179..1189") 
COMMENT        #12=(CFSignal (CObList) "\337-globin polyA" 43 0 0 1219 1310 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "1219..1310") 
COMMENT        #13=(CFSignal (CObList) "3' FR of \337-globin" 21 0 0 1311 1667 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "1311..1667") 
COMMENT        #14=(CFSignal (CObList) "lac promoter" 30 0 1 1745 1827 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1745..1827)") 
COMMENT        #15=(CFSignal (CObList) "SV40 ori" 33 0 0 1969 2044 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1969..2044") 
COMMENT        #16=(CFSignal (CObList) "SV40 polyA early" 43 0 0 2075 2149 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "2075..2149") 
COMMENT        #17=(CFSignal (CObList) "SV40 polyA late" 43 0 1 2075 2149 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(2075..2149)") 
COMMENT        #18=(CFSignal (CObList) "pMB1 ori" 33 0 1 2264 2665 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2264..2665)") 
COMMENT        #19=(CFSignal (CObList) "ampicillin" 4 0 1 3206 4066 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3206..4066)") 
COMMENT        #20=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 4202 4579 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4202..4579") 
COMMENT        #21=(CFSignal (CObList) "AG promoter" 29 0 0 4585 5926 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4585..5926") 
COMMENT        #22=(CFSignal (CObList) "intron AG promoter" 15 0 0 4954 5878 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4954..5878")) (CObList) 
COMMENT       (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #23=(CFSignal (CObList) "LMBP 3818" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #24=(CFSignal (CObList) "Last modified: 31-MAR-2008" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
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COMMENT                   5 "" 0 4) 
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COMMENT                    (CObjectList 
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COMMENT                          "DNA Plasmid 'pCAGGS-mCASP-7'" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 3818" 
COMMENT                          1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
COMMENT                          1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Creation Date: ? (no data)" 1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 03/31/08 09:44" 1) 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5929 bp" 1) 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Original Source Database: GenBank" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #38=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 5 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #40=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "EMAIL: <a href=\"mailto:bccm.genecorner@UGent.be\">bccm.lmbp@UGent.be</a>" 
COMMENT                          1))) "Original Author") 
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COMMENT                     #43=(CFolderPar 
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COMMENT                           (CObjectList 
COMMENT                            #44=(CLinePar (CParagraph 0 (15 #23# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "LMBP 3818") 
COMMENT                     #45=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #46=(CLinePar (CParagraph 0 (15 #24# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "Last modified: 31-MAR-2008"))) 
COMMENT                   "Annotations") 
COMMENT              #47=(CFolderPar 
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COMMENT                    (CObjectList 
COMMENT                     #48=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #49=(CFolderPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #50=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 267   End: 1178" 1) 
COMMENT                                   #51=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "mouse caspase-7 coding sequence" 1) 
COMMENT                                   #52=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #53=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   267..1178" 1))) "mCASP-7") 
COMMENT                            #54=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3206 (3 #19# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #55=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3206  End: 4066 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #56=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "antibiotic resistance gene; coding sequence" 
COMMENT                                        1) 
COMMENT                                   #57=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #58=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(3206..4066)" 1))) 
COMMENT                                 "ampicillin"))) "CDS (2 total)") 
COMMENT                     #59=(CFolderPar 
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COMMENT                           (CObjectList 
COMMENT                            #60=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4954 (3 #22# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #61=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4954  End: 5878" 1) 
COMMENT                                   #62=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "chicken \337-actin/rabbit \337-globin hybrid intron" 
COMMENT                                        1) 
COMMENT                                   #63=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #64=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4954..5878" 1))) 
COMMENT                                 "intron AG promoter"))) "Intron (1 total)") 
COMMENT                     #65=(CFolderPar 
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COMMENT                            #66=(CFolderPar 
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COMMENT                                  (CParagraph 78 (3 #9# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #67=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 78    End: 266 " 1) 
COMMENT                                   #68=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "5' UTR of the mouse caspase-7 gene" 1) 
COMMENT                                   #69=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #70=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   78..266" 1))) "5' UTR of mCASP-7") 
COMMENT                            #71=(CFolderPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1179  End: 1189" 1) 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "3' UTR of the mouse caspase-7 gene" 1) 
COMMENT                                   #74=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #75=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1179..1189" 1))) "3' UTR of mCASP-7") 
COMMENT                            #76=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1311 (3 #13# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1311  End: 1667" 1) 
COMMENT                                   #78=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "3' flanking region of the rabbit \337-globin gene" 
COMMENT                                        1) 
COMMENT                                   #79=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #80=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1311..1667" 1))) 
COMMENT                                 "3' FR of \337-globin"))) 
COMMENT                          "Misc. Feature (3 total)") 
COMMENT                     #81=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #82=(CFolderPar 
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COMMENT                                   #83=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4202  End: 4579" 1) 
COMMENT                                   #84=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "human CMV-IE enhancer" 1) 
COMMENT                                   #85=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #86=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4202..4579" 1))) "hCMV-IE enhancer") 
COMMENT                            #87=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4585 (3 #21# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #88=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4585  End: 5926" 1) 
COMMENT                                   #89=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "chicken \337-actin/rabbit \337-globin hybrid promoter" 
COMMENT                                        1) 
COMMENT                                   #90=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #91=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4585..5926" 1))) "AG promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
COMMENT                     #92=(CFolderPar 
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COMMENT                           (CObjectList 
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COMMENT                                   #94=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1745  End: 1827 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #95=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "E. coli lac operon promoter" 1) 
COMMENT                                   #96=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #97=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(1745..1827)" 1))) 
COMMENT                                 "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
COMMENT                     #98=(CFolderPar 
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COMMENT                           (CObjectList 
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COMMENT                                  (CObjectList 
COMMENT                                   #100=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "Start: 1969  End: 2044" 1) 
COMMENT                                   #101=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "bidirectional origin of SV40" 1) 
COMMENT                                   #102=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "Original Location Description:" 1) 
COMMENT                                   #103=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "   1969..2044" 1))) "SV40 ori") 
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COMMENT                                   (CObjectList 
COMMENT                                    #105=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Start: 2264  End: 2665 (Complementary)" 
COMMENT                                          1) 
COMMENT                                    #106=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "origin plasmid" 1) 
COMMENT                                    #107=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Original Location Description:" 1) 
COMMENT                                    #108=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "   complement(2264..2665)" 1))) 
COMMENT                                  "pMB1 ori"))) "Replication Origin (2 total)") 
COMMENT                     #109=(CFolderPar 
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COMMENT                            (CObjectList 
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COMMENT                                    (CObjectList 
COMMENT                                     #111=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 1219  End: 1310" 1) 
COMMENT                                     #112=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "3' UTR of the rabbit \337-globin gene, incl. polyA" 
COMMENT                                           1) 
COMMENT                                     #113=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #114=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   1219..1310" 1))) 
COMMENT                                   "\337-globin polyA") 
COMMENT                             #115=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 2075 (3 #16# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #116=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 2075  End: 2149" 1) 
COMMENT                                     #117=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "polyA signal of the SV40 early region" 
COMMENT                                           1) 
COMMENT                                     #118=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #119=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
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FEATURES             Location/Qualifiers
     misc_feature    78..266
                     /vntifkey="21"
                     /label=5'\UTR\of\mCASP-7
                     /note="5' UTR of the mouse caspase-7 gene"
     CDS             267..1178
                     /vntifkey="4"
                     /label=mCASP-7
                     /note="mouse caspase-7 coding sequence"
     misc_feature    1179..1189
                     /vntifkey="21"
                     /label=3'\UTR\of\mCASP-7
                     /note="3' UTR of the mouse caspase-7 gene"
     terminator      1219..1310
                     /vntifkey="43"
                     /label=ß-globin\polyA
                     /note="3' UTR of the rabbit ß-globin gene, incl. polyA"
     misc_feature    1311..1667
                     /vntifkey="21"
                     /label=3'\FR\of\ß-globin
                     /note="3' flanking region of the rabbit ß-globin gene"
     promoter        complement(1745..1827)
                     /vntifkey="30"
                     /label=lac\promoter
                     /note="E. coli lac operon promoter"
     rep_origin      1969..2044
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="bidirectional origin of SV40"
     terminator      2075..2149
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     terminator      complement(2075..2149)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     rep_origin      complement(2264..2665)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     CDS             complement(3206..4066)
                     /vntifkey="4"
                     /label=ampicillin
                     /note="antibiotic resistance gene; coding sequence"
     promoter        4202..4579
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
                     /note="human CMV-IE enhancer"
     promoter        4585..5926
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     intron          4954..5878
                     /vntifkey="15"
                     /label=intron\AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid intron"
BASE COUNT     1263 a      1606 c      1646 g      1414 t 
ORIGIN
        1 ttcctcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg 
       61 ggggaattcg gcacgaggcg gcgggctccg tagcgcgcgt tcccgctcgc ccccagcttt 
      121 cccgggctgc aggcacagcc ccgtccggcc cgcccgctcc tccccctccg cctccgggac 
      181 ttttgctttc agttttccca aagctgccct cgacccttgc ggaggacgga cgctgccgtg 
      241 ggctcctggc cgccgccgtg ggaacgatga ccgatgatca ggactgtgct gcggagctgg 
      301 aaaaggtgga ttcttccagc gaagacggag ttgacgccaa gccagaccgc tcctctatca 
      361 tctcctctat tctcttgaag aagaagagaa atgcctctgc gggccccgtc aggaccggcc 
      421 gggaccgagt gcccacttat ctgtaccgca tggatttcca gaagatgggt aaatgcatca 
      481 tcataaacaa caagaacttc gacaaagcga caggtatgga cgtccggaat gggacggaca 
      541 aagatgcagg ggccctcttc aagtgcttcc aaaacctggg ttttgaagta accgtccaca 
      601 atgactgctc ttgtgcaaag atgcaagatc tgcttagaaa agcctctgag gaggaccaca 
      661 gcaactcggc ctgcttcgcc tgcgtcctgc tgagccacgg ggaagaggac ctgatttacg 
      721 ggaaagatgg cgtgacaccc ataaaggatc tgacagctca ttttagggga gaccgatgca 
      781 aaaccctgtt agagaaaccc aaactcttct tcattcaggc atgccgaggg acggagctcg 
      841 atgatggaat ccaggctgac tcggggccca tcaacgacat tgacgctaat ccccgcaaca 
      901 agatcccggt ggaagccgac ttcctctttg cttactccac ggttccaggt tattactcat 
      961 ggaggaaccc agggaaaggc tcctggtttg tgcaggccct ctgctccatc ctgaatgagc 
     1021 atggcaagga cctcgagatc atgcagatcc tgaccagggt gaacgacagg gtggccaggc 
     1081 acttcgagtc ccagtctgat gatccacgct tcaacgagaa gaagcagatc ccgtgtatgg 
     1141 tgtccatgct caccaaagag ctgtacttca gccgttgacc acccttcagc caatgccctg 
     1201 gctcacaaat accactgaga tctttttccc tctgccaaaa attatgggga catcatgaag 
     1261 ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt 
     1321 tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat 
     1381 cagaatgagt atttggttta gagtttggca acatatgccc atatgctggc tgccatgaac 
     1441 aaaggttggc tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta 
     1501 ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt 
     1561 ttttctttaa catccctaaa attttcctta catgttttac tagccagatt tttcctcctc 
     1621 tcctgactac tcccagtcat agctgtccct cttctcttat ggagatccct cgacctgcag 
     1681 cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 
     1741 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctgggtgc ctaatgagtg 
     1801 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 
     1861 tgccagcgga tccgcatctc aattagtcag caaccatagt cccgccccta actccgccca 
     1921 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 
     1981 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag 
     2041 gcttttttgg aggcctaggc ttttgcaaaa agctaacttg tttattgcag cttataatgg 
     2101 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 
     2161 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc gctgcattaa 
     2221 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg 
     2281 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 
     2341 gcggtaatac ggttatccac agaatcaggg ataacgcagg aaagaacatg tgagcaaaag 
     2401 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 
     2461 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 
     2521 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 
     2581 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 
     2641 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 
     2701 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 
     2761 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 
     2821 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 
     2881 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 
     2941 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 
     3001 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 
     3061 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 
     3121 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 
     3181 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 
     3241 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 
     3301 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 
     3361 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 
     3421 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 
     3481 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 
     3541 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 
     3601 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 
     3661 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 
     3721 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 
     3781 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 
     3841 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 
     3901 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 
     3961 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 
     4021 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 
     4081 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 
     4141 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgggt 
     4201 cgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc 
     4261 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc 
     4321 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg 
     4381 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat 
     4441 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc 
     4501 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta 
     4561 ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc 
     4621 tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg 
     4681 atgggggcgg gggggggggg ggggcccccc ccaggcgggg cggggcgggg cgaggggcgg 
     4741 ggcggggcga ggcggaaagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt 
     4801 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg 
     4861 ggagtcgttg cgcgctgcct tccccccgtg ccccgctccg ccgccgcctc gcgccgcccg 
     4921 ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct 
     4981 ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa 
     5041 agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg gggtgcgtgc 
     5101 gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc 
     5161 tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg 
     5221 ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg 
     5281 tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac 
     5341 ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt 
     5401 ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg 
     5461 gggccgcctc gggccgggga gggctcgggg gaaggggcgc ggcggccccc ggagcgccgg 
     5521 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc 
     5581 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac 
     5641 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga 
     5701 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc 
     5761 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt 
     5821 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct 
     5881 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaa 
//
