ID pCAGGSmIL1B standard; circular DNA; ; 5633 BP. DE LMBP 3853. CC Accession number: LMBP 3853 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 27-NOV-2007 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT CDS 76..885 FT /vntifkey="4" FT /label=mIL1B FT terminator 923..1014 FT /vntifkey="43" FT /label=ß-globin\polyA FT /note="3' UTR of the rabbit ß-globin gene, incl. polyA" FT misc_feature 1015..1371 FT /vntifkey="21" FT /label=3'\FR\of\ß-globin FT /note="3' flanking region of the rabbit ß-globin gene" FT promoter complement(1449..1531) FT /vntifkey="30" FT /label=lac\promoter FT /note="E. coli lac operon promoter" FT rep_origin 1673..1748 FT /vntifkey="33" FT /label=SV40\ori FT /note="bidirectional origin of SV40" FT terminator 1779..1853 FT /vntifkey="43" FT /label=SV40\polyA\early FT /note="polyA signal of the SV40 early region" FT terminator complement(1779..1853) FT /vntifkey="43" FT /label=SV40\polyA\late FT /note="polyA signal of the SV40 late region" FT rep_origin complement(1968..2369) FT /vntifkey="33" FT /label=pMB1\ori FT CDS complement(2910..3770) FT /vntifkey="4" FT /label=ampicillin FT /note="antibiotic resistance gene; coding sequence" FT promoter 3906..4283 FT /vntifkey="29" FT /label=hCMV-IE\enhancer FT promoter 4289..5630 FT /vntifkey="29" FT /label=AG\promoter FT intron 4658..5582 FT /vntifkey="15" FT /label=intron\AG\promoter SQ Sequence 5633 BP; 1243 A; 1481 C; 1529 G; 1380 t; ttcctcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg 60 gatcagcgct ccaccatggc aactgttcct gaactcaact gtgaaatgcc accttttgac 120 agtgatgaga atgacctgtt ctttgaagtt gacggacccc aaaagatgaa gggctgcttc 180 caaacctttg acctgggctg tcctgatgag agcatccagc ttcaaatctc gcagcagcac 240 atcaacaaga gcttcaggca ggcagtatca ctcattgtgg ctgtggagaa gctgtggcag 300 ctacctgtgt ctttcccgtg gaccttccag gatgaggaca tgagcacctt cttttccttc 360 atctttgaag aagagcccat cctctgtgac tcatgggatg atgatgataa cctgctggtg 420 tgtgacgttc ccattagaca actgcactac aggctccgag atgaacaact aaaaagcctc 480 gtgctgtcgg acccatatga gctgaaagct ctccacctca atggacagaa tatcaaccaa 540 caagtgatat tctccatgag ctttgtacaa ggagaaccaa gcaacgacaa aatacctgtg 600 gccttgggcc tcaaaggaaa gaatctatac ctgtcctgtg taatgaaaga cggcacaccc 660 accctgcagc tggagagtgt ggatcccaag caatacccaa agaagaagat ggaaaaacgg 720 tttgtcttca acaagataga agtcaagagc aaagtggagt ttgagtctgc agagttcccc 780 aactggtaca tcagcacctc acaagcagag cacaagcctg tcttcctggg aaacaacagt 840 ggtcaggaca taattgactt caccatggaa tctgtgtctt cctaagcgct gatccaatgc 900 cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat 960 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt 1020 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa 1080 acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat 1140 gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc 1200 cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt 1260 atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct 1320 cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct 1380 gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc 1440 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg gtgcctaatg 1500 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct 1560 gtcgtgccag cggatccgca tctcaattag tcagcaacca tagtcccgcc cctaactccg 1620 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 1680 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga 1740 ggaggctttt ttggaggcct aggcttttgc aaaaagctaa cttgtttatt gcagcttata 1800 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc 1860 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccgctgca 1920 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 1980 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 2040 aaaggcggta atacggttat ccacagaatc agggataacg caggaaagaa catgtgagca 2100 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 2160 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 2220 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 2280 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 2340 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 2400 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 2460 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 2520 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 2580 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 2640 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 2700 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 2760 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 2820 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 2880 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc 2940 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 3000 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc 3060 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 3120 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 3180 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg 3240 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt 3300 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc 3360 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt 3420 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc 3480 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc 3540 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 3600 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac 3660 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa 3720 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt 3780 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa 3840 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct 3900 gggtcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca 3960 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc 4020 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat 4080 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt 4140 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc 4200 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta 4260 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc 4320 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc 4380 agcgatgggg gcgggggggg ggggggggcc ccccccaggc ggggcggggc ggggcgaggg 4440 gcggggcggg gcgaggcgga aaggtgcggc ggcagccaat cagagcggcg cgctccgaaa 4500 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg 4560 ggcgggagtc gttgcgcgct gccttccccc cgtgccccgc tccgccgccg cctcgcgccg 4620 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc 4680 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg 4740 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc 4800 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga 4860 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc 4920 cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg 4980 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct 5040 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg 5100 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg 5160 ggcggggccg cctcgggccg gggagggctc gggggaaggg gcgcggcggc ccccggagcg 5220 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga 5280 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc 5340 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg 5400 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct 5460 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc 5520 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac 5580 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaa 5633 //