LOCUS       pCAGGS-EmCASP-1         6079 bp    DNA     circular      2-DEC-2009
DEFINITION  LMBP 3912.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 3912
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 2-DEC-2009
            
            
COMMENT     VNTNAME|pCAGGS-EmCASP-1|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-EmCASP-1" 0 6079 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "BglII" "NcoI" "NotI" "SphI" "XbaI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 6079 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "BglII" "agatct" 2 0 1 1369 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 3 12 0 2112 0 4728 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 57 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "SphI" "gcatgc" 6 0 1 909 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 5982 0 0 "")) 
COMMENT       (CObList 
COMMENT        #5=(CFSignal (CObList) "ampicillin resistance" 4 0 1 3356 4216 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(3356..4216)") 
COMMENT        #6=(CFSignal (CObList) "E-tag" 22 0 0 16 54 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "16..54") 
COMMENT        #7=(CFSignal (CObList) "mCASP-1" 4 0 0 61 1266 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "61..1263") 
COMMENT        #8=(CFSignal (CObList) "3' UTR of rabbit \337-globin, incl. polyA" 43 0 
COMMENT            0 1369 1460 0 (CStringList) (CStringList) 1 1 1 1 "1369..1460") 
COMMENT        #9=(CFSignal (CObList) "lac promoter" 30 0 1 1895 1977 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1895..1977)") 
COMMENT        #10=(CFSignal (CObList) "SV40 ori" 33 0 0 2119 2194 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2119..2194") 
COMMENT        #11=(CFSignal (CObList) "SV40 polyA early" 43 0 0 2225 2299 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "2224..2299") 
COMMENT        #12=(CFSignal (CObList) "SV40 polyA late" 43 0 1 2225 2299 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(2224..2299)") 
COMMENT        #13=(CFSignal (CObList) "pMB1 ori" 33 0 1 2414 2815 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2414..2815)") 
COMMENT        #14=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 4352 4729 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4352..4729") 
COMMENT        #15=(CFSignal (CObList) "intron AG promoter" 15 0 0 5104 6028 0 
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COMMENT        #16=(CFSignal (CObList) "AG promoter" 29 0 0 4735 6076 0 (CStringList) 
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COMMENT        #17=(CFSignal (CObList) "3' flanking region of rabbit \337-globin" 21 0 
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COMMENT             (CStringList) (CStringList) 1 1 0 0 "") 
COMMENT        #19=(CFSignal (CObList) "LMBP 3912" 2 0 0 0 0 0 (CStringList) 
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COMMENT                          "DNA Plasmid 'pCAGGS-EmCASP-1'" 1) 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
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COMMENT                          "Creation Date: ? (no data)" 1) 
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COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6079 bp" 1) 
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COMMENT                   "Standard Fields") 
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COMMENT                          "Promoter Eukaryotic (2 total)") 
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COMMENT                                        "   complement(2414..2815)" 1))) 
COMMENT                                 "pMB1 ori"))) "Replication Origin (2 total)") 
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COMMENT                   "Feature Map") 
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FEATURES             Location/Qualifiers
     CDS             complement(3356..4216)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     misc_marker     16..54
                     /vntifkey="22"
                     /label=E-tag
     CDS             61..1266
                     /vntifkey="4"
                     /label=mCASP-1
     terminator      1369..1460
                     /vntifkey="43"
                     /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA
     promoter        complement(1895..1977)
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      2119..2194
                     /vntifkey="33"
                     /label=SV40\ori
     terminator      2225..2299
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(2225..2299)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      complement(2414..2815)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        4352..4729
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
     intron          5104..6028
                     /vntifkey="15"
                     /label=intron\AG\promoter
     promoter        4735..6076
                     /vntifkey="29"
                     /label=AG\promoter
     misc_feature    1461..1817
                     /vntifkey="21"
                     /label=3'\flanking\region\of\rabbit\ß-globin
BASE COUNT     1403 a      1529 c      1635 g      1512 t 
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 
       61 gctgacaaga tcctgagggc aaagaggaag caatttatca actcagtgag tatagggaca 
      121 ataaatggat tgttggatga acttttagag aagagagtgc tgaatcagga agaaatggat 
      181 aaaataaaac ttgcaaacat tactgctatg gacaaggcac gggacctatg tgatcatgtc 
      241 tctaaaaaag ggccccaggc aagccaaatc tttatcactt acatttgtaa tgaagactgc 
      301 tacctggcag gaattctgga gcttcaatca gctccatcag ctgaaacatt tgttgctaca 
      361 gaagattcta aaggaggaca tccttcatcc tcagaaacaa aggaagaaca gaacaaagaa 
      421 gatggcacat ttccaggact gactgggacc ctcaagtttt gccctttaga aaaagcccag 
      481 aagttatgga aagaaaatcc ttcagagatt tatccaataa tgaatacaac cactcgtaca 
      541 cgtcttgccc tcattatctg caacacagag tttcaacatc tttctccgag ggttggagct 
      601 caagttgacc tcagagaaat gaagttgctg ctggaggatc tggggtatac cgtgaaagtg 
      661 aaagaaaatc tcacagctct ggagatggtg aaagaggtga aagaatttgc tgcctgccca 
      721 gagcacaaga cttctgacag tactttcctt gtattcatgt ctcatggtat ccaggaggga 
      781 atatgtggga ccacatactc taatgaagtt tcagatattt taaaggttga cacaatcttt 
      841 cagatgatga acactttgaa gtgcccaagc ttgaaagaca agcccaaggt gatcattatt 
      901 caggcatgcc gtggagagaa acaaggagtg gtgttgttaa aagattcagt aagagactct 
      961 gaagaggatt tcttaacgga tgcaattttt gaagatgatg gcattaagaa ggcccatata 
     1021 gagaaagatt ttattgcttt ctgctcttca acaccagata atgtgtcttg gagacatcct 
     1081 gtcaggggct cacttttcat tgagtcactc atcaaacaca tgaaagaata tgcctggtct 
     1141 tgtgacttgg aggacatttt cagaaaggtt cgattttcat ttgaacaacc agaatttagg 
     1201 ctacagatgc ccactgctga tagggtgacc ctgacaaaac gtttctacct cttcccggga 
     1261 cattaaacgc gtgggtaccg gatccggcaa ttcactcctc aggtgcaggc tgcctatcag 
     1321 aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc 
     1381 tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa 
     1441 aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg 
     1501 acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca 
     1561 acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat 
     1621 atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag 
     1681 atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta 
     1741 catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct 
     1801 cttctcttat ggagatccct cgacctgcag cccaagcttg gcgtaatcat ggtcatagct 
     1861 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat 
     1921 aaagtgtaaa gcctgggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca 
     1981 ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga tccgcatctc aattagtcag 
     2041 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc 
     2101 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg 
     2161 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa 
     2221 agctaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 
     2281 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 
     2341 atcttatcat gtctggatcc gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt 
     2401 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg 
     2461 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg 
     2521 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 
     2581 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 
     2641 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 
     2701 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 
     2761 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 
     2821 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 
     2881 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 
     2941 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 
     3001 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc 
     3061 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 
     3121 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 
     3181 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 
     3241 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 
     3301 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 
     3361 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 
     3421 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 
     3481 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 
     3541 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 
     3601 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 
     3661 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 
     3721 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 
     3781 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 
     3841 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 
     3901 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 
     3961 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 
     4021 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 
     4081 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 
     4141 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 
     4201 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 
     4261 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 
     4321 gcacatttcc ccgaaaagtg ccacctgggt cgacattgat tattgactag ttattaatag 
     4381 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt 
     4441 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg 
     4501 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat 
     4561 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct 
     4621 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg 
     4681 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtcgaggtg 
     4741 agccccacgt tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat 
     4801 ttatttattt tttaattatt ttgtgcagcg atgggggcgg gggggggggg ggggcccccc 
     4861 ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga ggcggaaagg tgcggcggca 
     4921 gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg 
     4981 ccctataaaa agcgaagcgc gcggcgggcg ggagtcgttg cgcgctgcct tccccccgtg 
     5041 ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca 
     5101 caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt ggtttaatga 
     5161 cggcttgttt cttttctgtg gctgcgtgaa agccttgagg ggctccggga gggccctttg 
     5221 tgcgggggga gcggctcggg gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc 
     5281 ggctccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc 
     5341 cgcagtgtgc gcgaggggag cgcggccggg ggcggtgccc cgcggtgcgg ggggggctgc 
     5401 gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg ggtgagcagg gggtgtgggc 
     5461 gcgtcggtcg ggctgcaacc ccccctgcac ccccctcccc gagttgctga gcacggcccg 
     5521 gcttcgggtg cggggctccg tacggggcgt ggcgcggggc tcgccgtgcc gggcgggggg 
     5581 tggcggcagg tgggggtgcc gggcggggcg gggccgcctc gggccgggga gggctcgggg 
     5641 gaaggggcgc ggcggccccc ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca 
     5701 ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc aaatctgtgc 
     5761 ggagccgaaa tctgggaggc gccgccgcac cccctctagc gggcgcgggg cgaagcggtg 
     5821 cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc 
     5881 ccttctccct ctccagcctc ggggctgtcc gcggggggac ggctgccttc gggggggacg 
     5941 gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct ctgctaacca 
     6001 tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta ttgtgctgtc 
     6061 tcatcatttt ggcaaagaa 
//
