LOCUS       pAS2mCASP-11m           9519 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 3922.
SOURCE      
  ORGANISM  
COMMENT     (800)357-3114 or http://www.informaxinc.com/
COMMENT     VNTNAME|pAS2mCASP-11m|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pAS2mCASP-11m" 0 9519 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 " all enzymes of LMBP" 1 1 1 1 0 0 1 1 0 1 10 6 
COMMENT        4000 50 0 1 0 (CHomObj 0 0 0 3 75) 
COMMENT        (CWordArray 0 4 15 20 21 22 29 30 33 43) (CWordArray) 
COMMENT        (CStringList "BalI" "EcoRI" "NcoI" "NheI" "PstI" "SalI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 1 9519 1 9519 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 3951 0 5946 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 2 6010 0 6263 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "SalI" "gtcgac" 2 0 1 7131 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "BalI" "tggcca" 4 0 1 6003 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "NheI" "gctagc" 2 0 2 5982 0 6768 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 7141 0 0 "")) 
COMMENT       (CObList 
COMMENT        #6=(CFSignal (CObList) "2mu ori" 33 0 1 2 982 0 (CStringList) 
COMMENT            (CStringList) 1 1 0 0 "") 
COMMENT        #7=(CFSignal (CObList) "f1 ori" 33 0 1 2661 3116 0 (CStringList) 
COMMENT            (CStringList) 1 1 0 0 "") 
COMMENT        #8=(CFSignal (CObList) "ADH1 promoter" 29 0 0 5075 5478 0 (CStringList) 
COMMENT            (CStringList) 1 1 0 0 "") 
COMMENT        #9=(CFSignal (CObList) "GALbd" 4 0 0 5501 5941 0 (CStringList) 
COMMENT            (CStringList) 1 1 0 0 "") 
COMMENT        #10=(CFSignal (CObList) "HA epitope" 22 0 0 5957 5983 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #11=(CFSignal (CObList) "mCASP-11m" 4 0 0 6011 7129 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #12=(CFSignal (CObList) "ADH1 terminator" 43 0 0 7157 7258 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "") 
COMMENT        #13=(CFSignal (CObList) "pMB1 ori" 33 0 1 7586 7988 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #14=(CFSignal (CObList) "AMP" 4 0 1 8529 9389 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #15=(CFSignal (CObList) "TRP1" 4 0 0 1884 2558 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #16=(CFSignal (CObList) "CYH2" 4 0 1 3855 4304 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "")) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #17=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #18=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 2 0 1 178) 
COMMENT                    (CObjectList 
COMMENT                     #19=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid pAS2mCASP-11m" 0) 
COMMENT                     #20=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DMB 3922  (P. Schotte)" 0) 
COMMENT                     #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 9519 bp" 0) 
COMMENT                     #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 0) 
COMMENT                     #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 0))) "General Description") 
COMMENT              #24=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 10 (6 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #25=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 2 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #26=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1884 (3 #15# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #27=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1884  End: 2558" 0) 
COMMENT                                   #28=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) "" 0))) 
COMMENT                                 "TRP1") 
COMMENT                            #29=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3855 (3 #16# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #30=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3855  End: 4304 (Complementary)" 
COMMENT                                        0) 
COMMENT                                   #31=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) "" 0))) 
COMMENT                                 "CYH2") 
COMMENT                            #32=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5501 (3 #9# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #33=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5501  End: 5941" 0) 
COMMENT                                   #34=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) "" 0))) 
COMMENT                                 "GALbd") 
COMMENT                            #35=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 6011 (3 #11# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #36=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 6011  End: 7129" 0) 
COMMENT                                   #37=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) "" 0))) 
COMMENT                                 "mCASP-11m") 
COMMENT                            #38=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 8529 (3 #14# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #39=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 8529  End: 9389 (Complementary)" 
COMMENT                                        0) 
COMMENT                                   #40=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) "" 0))) 
COMMENT                                 "AMP"))) "CDS (5 signals)") 
COMMENT                     #41=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 22 (7 22 0) 1 2 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #42=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5957 (3 #10# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #43=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5957  End: 5983" 0) 
COMMENT                                   #44=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) "" 0))) 
COMMENT                                 "HA epitope"))) "Misc_marker (1 signal)") 
COMMENT                     #45=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 29 (7 29 0) 1 2 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #46=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5075 (3 #8# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #47=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5075  End: 5478" 0) 
COMMENT                                   #48=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) "" 0))) 
COMMENT                                 "ADH1 promoter"))) 
COMMENT                          "Promoter_eukaryotic (1 signal)") 
COMMENT                     #49=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 33 (7 33 0) 1 2 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #50=(CFolderPar 
COMMENT                                 (CGroupPar (CParagraph 2 (3 #6# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #51=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2     End: 982  (Complementary)" 
COMMENT                                        0) 
COMMENT                                   #52=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) "" 0))) 
COMMENT                                 "2mu ori") 
COMMENT                            #53=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2661 (3 #7# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #54=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2661  End: 3116 (Complementary)" 
COMMENT                                        0) 
COMMENT                                   #55=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) "" 0))) 
COMMENT                                 "f1 ori") 
COMMENT                            #56=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 7586 (3 #13# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #57=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 7586  End: 7988 (Complementary)" 
COMMENT                                        0) 
COMMENT                                   #58=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) "" 0))) 
COMMENT                                 "pMB1 ori"))) "Rep_origin (3 signals)") 
COMMENT                     #59=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 43 (7 43 0) 1 2 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #60=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 7157 (3 #12# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #61=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 7157  End: 7258" 0) 
COMMENT                                   #62=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) "" 0))) 
COMMENT                                 "ADH1 terminator"))) "Terminator (1 signal)"))) 
COMMENT                   "Functional Map") 
COMMENT              #63=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 11 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #64=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 11359300 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #65=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #3# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #66=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #3# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 6003 " 0))))) "BalI: 1 site") 3 3 
COMMENT                          "TGGCCA" "ACCGGT") 
COMMENT                     #67=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 11470436 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #68=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #0# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #69=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #0# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 3951 " 0) 
COMMENT                                   #70=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #0# 2) 1 2 2 0 191) 
COMMENT                                        " N2: 5946 " 0))))) "EcoRI: 2 sites") 1 
COMMENT                          5 "GAATTC" "CTTAAG") 
COMMENT                     #71=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 11776500 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #72=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #1# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #1# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 6010 " 0) 
COMMENT                                   #74=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #1# 2) 1 2 2 0 191) 
COMMENT                                        " N2: 6263 " 0))))) "NcoI: 2 sites") 1 5 
COMMENT                          "CCATGG" "GGTACC") 
COMMENT                     #75=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 12000164 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #76=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #4# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #4# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 5982 " 0) 
COMMENT                                   #78=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #4# 2) 1 2 2 0 191) 
COMMENT                                        " N2: 6768 " 0))))) "NheI: 2 sites") 1 5 
COMMENT                          "GCTAGC" "CGATCG") 
COMMENT                     #79=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 11037292 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #80=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #5# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #81=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #5# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 7141 " 0))))) "PstI: 1 site") 5 1 
COMMENT                          "CTGCAG" "GACGTC") 
COMMENT                     #82=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 11598636 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #83=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #2# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #84=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #2# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 7131 " 0))))) "SalI: 1 site") 1 5 
COMMENT                          "GTCGAC" "CAGCTG"))) "Restriction Map") 
COMMENT              #85=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 14 (0 0) 1 2 0 0 178) (CObjectList)) 
COMMENT                   "Annotations")))) 
COMMENT       (CGraphView 
COMMENT        (CWStyleSheet 
COMMENT         (CObjectList 
COMMENT          #86=(CWidgetStyle "Annotation" 0 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 0 
COMMENT               0) 
COMMENT          #87=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.611111 
COMMENT               0 1 5 "@N (@S)" 0) 
COMMENT          #88=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 
COMMENT               (LOGBRUSH 0 16777215 0) 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 1 
COMMENT               2 "?" 0) 
COMMENT          #89=(CWidgetStyle "Mark 1" 0 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" 0) 
COMMENT          #90=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.64 0 1 -1 
COMMENT               "@N (@H%)" 0) 
COMMENT          #91=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.611111 
COMMENT               0 1 5 "@N (@S)" 0) 
COMMENT          #92=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT               "@N" 0) 
COMMENT          #93=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 
COMMENT               "@F bp (molecule @L bp)" 0) 
COMMENT          #94=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 
COMMENT               "Fragment of @N" 0) 
COMMENT          #95=(CWidgetStyle "Molecule Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 16 
COMMENT               "@L bp" 0) 
COMMENT          #96=(CWidgetStyle "Molecule Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 "@N" 0) 
COMMENT          #97=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 
COMMENT               (LOGBRUSH 2 8388608 5) 0 0 0) 
COMMENT          #98=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 255 0) 0 
COMMENT               0 0) 
COMMENT          #99=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 
COMMENT               0 0) 
COMMENT          #100=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 0 0 0) 
COMMENT          #101=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 
COMMENT                0) 
COMMENT          #102=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 0) 2 1 (LOGBRUSH 0 12632256 0) 
COMMENT                0 0 0) #103=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #104=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) 
COMMENT          #105=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #106=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #107=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) 
COMMENT          #108=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) 
COMMENT          #109=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #110=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #111=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 8388608) 10 0 0 0 0) 
COMMENT          #112=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #113=(CWidgetStyle "Void" 0 0 0 0 0) 
COMMENT          #114=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                1 3 "@T @N " 0) 
COMMENT          #115=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #116=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #117=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #118=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0)) 0.164644 1.74233 0.164644 2.53336 
COMMENT         (15 (CShapeMapEntry 0 "Line 1" 1 "Position Label") 9 
COMMENT          (CShapeMapEntry 0 "Shape 4" 1 "Position Label") 4 
COMMENT          (CShapeMapEntry 0 "Shape 3" 1 "Position Label") 30 
COMMENT          (CShapeMapEntry 0 "Shape 1" 1 "Position Label") 29 
COMMENT          (CShapeMapEntry 0 "Shape 1" 1 "Position Label") 26 
COMMENT          (CShapeMapEntry 0 "Line 0" 1 "Position Label") 25 
COMMENT          (CShapeMapEntry 0 "Line 4" 1 "Position Label") 22 
COMMENT          (CShapeMapEntry 0 "Shape 2" 1 "Position Label") 21 
COMMENT          (CShapeMapEntry 5 "Line 2" 1 "Position Label") 43 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Position Label") 33 
COMMENT          (CShapeMapEntry 0 "Shape 4" 1 "Position Label") 32 
COMMENT          (CShapeMapEntry 0 "Line 3" 1 "Position Label") 52 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Position Label")) 39 39 39 39 0.1 0) 1 1 
COMMENT        0 1 1 (mapper: 20.3628 -14.2384 39 39 0.01 10 -1 9519 9519 1 0 0) 
COMMENT        #119=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil 3 100) 
COMMENT              (CObjectList 
COMMENT               #120=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -449 100) 
COMMENT                     (CObjectList 
COMMENT                      #121=(CAxis 
COMMENT                            (CWideLine 
COMMENT                             (CWidget 0 (0 0) 1 2 0 0 #102# 11839892 0) 
COMMENT                             (LOGPEN 0 0 0) 2 (LOGBRUSH 0 12632256 4294954445) 1 
COMMENT                             6.27484 6.27284 1 0.0214037) 0.0659017) 
COMMENT                      #122=(CLabel 
COMMENT                            (CWidget 1001 (0 0) 1 2 0 0 #96# 168650593 100) 
COMMENT                            (LOGPEN 0 0 8421376) 1 
COMMENT                            (LOGFONT 61 23 0 0 400 0 0 0 0 3 2 1 34 "Arial") 
COMMENT                            1.74233 0.91 0 "pAS2mCASP-11m" "@N" 1 0 0.871165 0 
COMMENT                            -10 11.6154 1.53846 Nil) 
COMMENT                      #123=(CLabel (CWidget 1002 (0 0) 1 2 0 0 #95# 6116508 100) 
COMMENT                            (LOGPEN 0 0 8421376) 1 
COMMENT                            (LOGFONT 70 26 0 0 400 0 0 0 0 3 2 1 49 
COMMENT                             "Courier New") 1.74233 1.05 0 "9519 bp" "@L bp" 16 
COMMENT                            0 -0.871165 0 -10.8 4.94872 1.79487 Nil)) 
COMMENT                     (CObjectList)) 
COMMENT               #124=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -789 100) 
COMMENT                     (CObjectList 
COMMENT                      #125=(CGroupWidget 
COMMENT                            (CWidget 4 (7 4 0) 1 2 0 0 Nil -925 100) 
COMMENT                            (CObjectList 
COMMENT                             #126=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #9# 0) 1 2 0 0 #98# 598 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    2.35796 2.64859 1 0.082322) 0.8 1.8 0) 
COMMENT                             #127=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #116# 6116508 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 42 15 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.63 8388608 "GALbd" "@N" 1 
COMMENT                                   -9.01391 -12.1963 0 -10 2.92308 1.10256 #126#) 
COMMENT                             #128=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #11# 0) 1 2 0 0 #98# 1 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    1.57505 2.31249 1 0.082322) 0.8 1.8 0) 
COMMENT                             #129=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #116# 10289568 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 42 15 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.63 8388608 "mCASP-11m" 
COMMENT                                   "@N" 1 -14.7179 -2.94872 0 -10 5.30769 
COMMENT                                   1.10256 #128#) 
COMMENT                             #130=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #14# 0) 1 2 0 0 #98# 103 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    0.0856722 0.653086 1 0.082322) 0.8 1.8 1) 
COMMENT                             #131=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #116# 10289728 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 42 15 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.63 8388608 "AMP" "@N" 1 
COMMENT                                   -5.74047 11.6591 0 -10 2.07692 1.10256 #130#) 
COMMENT                             #132=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #15# 0) 1 2 0 0 #98# 29300 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    4.58742 5.03226 1 0.082322) 0.8 1.8 0) 
COMMENT                             #133=(CLabel (CWidget 0 (0 0) 1 2 0 0 #116# 60 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 42 15 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.63 8388608 "TRP1" "@N" 1 
COMMENT                                   13.4699 1.33207 0 -10 2.41026 1.10256 #132#) 
COMMENT                             #134=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #16# 0) 1 2 0 0 #98# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    3.43677 3.73333 1 0.082322) 0.8 1.8 1) 
COMMENT                             #135=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #116# 6116508 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 42 15 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.63 8388608 "CYH2" "@N" 1 
COMMENT                                   6.87628 -12.4271 0 -10 2.41026 1.10256 #134#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #136=(CGroupWidget 
COMMENT                            (CWidget 22 (7 22 0) 1 2 0 0 Nil -925 100) 
COMMENT                            (CObjectList 
COMMENT                             #137=(CScratch 
COMMENT                                   (CWidget 0 (3 #10# 0) 1 2 0 0 #111# 431 100) 
COMMENT                                   (LOGPEN 0 6 8388608) 10 1 2.34808 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #138=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #116# 13344 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 42 15 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.63 8388608 "HA epitope" 
COMMENT                                   "@N" 1 -13.747 -9.27575 0 -10 4.64103 1.10256 
COMMENT                                   #137#)) (CObjectList)) 
COMMENT                      #139=(CGroupWidget 
COMMENT                            (CWidget 29 (7 29 0) 1 2 0 0 Nil -653 100) 
COMMENT                            (CObjectList 
COMMENT                             #140=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #8# 0) 1 2 0 0 #100# 598 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 8388736 0) 1 
COMMENT                                    2.66309 2.92933 1 0.082322) 0.8 1.8 0) 
COMMENT                             #141=(CLabel (CWidget 0 (0 0) 1 2 0 0 #116# 0 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 42 15 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.63 8388608 
COMMENT                                   "ADH1 promoter" "@N" 1 -7.91846 -13.9386 0 
COMMENT                                   -10 6.25641 1.10256 #140#)) (CObjectList)) 
COMMENT                      #142=(CGroupWidget 
COMMENT                            (CWidget 33 (7 33 0) 1 2 0 0 Nil -585 100) 
COMMENT                            (CObjectList 
COMMENT                             #143=(CWideLine 
COMMENT                                   (CWidget 0 (3 #6# 0) 1 2 0 0 #97# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 1 
COMMENT                                   5.62603 6.27253 1 0.082322) 
COMMENT                             #144=(CLabel (CWidget 0 (0 0) 1 2 0 0 #116# 0 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 42 15 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.63 8388608 "2mu ori" "@N" 
COMMENT                                   1 6.26366 11.8899 0 -10 3.17949 1.10256 #143#) 
COMMENT                             #145=(CWideLine 
COMMENT                                   (CWidget 0 (3 #7# 0) 1 2 0 0 #97# 12001096 
COMMENT                                    100) (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 
COMMENT                                   1 4.21969 4.5202 1 0.082322) 
COMMENT                             #146=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #116# 6116508 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 42 15 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.63 8388608 "f1 ori" "@N" 
COMMENT                                   1 12.6439 -4.53594 0 -10 2.15385 1.10256 
COMMENT                                   #145#) 
COMMENT                             #147=(CWideLine 
COMMENT                                   (CWidget 0 (3 #13# 0) 1 2 0 0 #97# 10289616 
COMMENT                                    100) (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 
COMMENT                                   1 1.00896 1.27454 1 0.082322) 
COMMENT                             #148=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #116# 6116508 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 42 15 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.63 8388608 "pMB1 ori" 
COMMENT                                   "@N" 1 -12.8456 5.9937 0 -10 3.76923 1.10256 
COMMENT                                   #147#)) (CObjectList)) 
COMMENT                      #149=(CGroupWidget 
COMMENT                            (CWidget 43 (7 43 0) 1 2 0 0 Nil -313 100) 
COMMENT                            (CObjectList 
COMMENT                             #150=(CLine 
COMMENT                                   (CWidget 0 (3 #12# 0) 1 2 0 0 #106# 711 100) 
COMMENT                                   (LOGPEN 0 6 16711680) 8 0.835356 1.49004 
COMMENT                                   1.55726) 
COMMENT                             #151=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #116# 1885431891 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 42 15 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.63 8388608 
COMMENT                                   "ADH1 terminator" "@N" 1 -15.1653 3.04624 0 
COMMENT                                   -10 6.76923 1.10256 #150#)) (CObjectList))) 
COMMENT                     (CObjectList)) 
COMMENT               #152=(CGroupWidget (CWidget 11 (8 0) 1 2 0 0 Nil -721 100) 
COMMENT                     (CObjectList 
COMMENT                      #153=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #2# 0) 1 2 0 0 Nil -178 100) 
COMMENT                            (CObjectList 
COMMENT                             #154=(CScratch 
COMMENT                                   (CWidget 1 (1 #2# 1) 1 2 0 0 #109# 6116508 
COMMENT                                    100) (LOGPEN 0 6 0) 8 1 1.57439 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #155=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #87# 10289784 100) 
COMMENT                                   (LOGPEN 0 0 8421376) 1 
COMMENT                                   (LOGFONT 41 15 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 0 
COMMENT                                   "{\\i Sal} I (7131)" "@N (@S)" 5 -13.7523 
COMMENT                                   -0.284571 0 -10 4.12821 1.02564 #154#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #156=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #3# 0) 1 2 0 0 Nil -654 100) 
COMMENT                            (CObjectList 
COMMENT                             #157=(CScratch 
COMMENT                                   (CWidget 1 (1 #3# 1) 1 2 0 0 #109# 389 100) 
COMMENT                                   (LOGPEN 0 6 0) 8 1 2.31776 1.9 0.082322 1) 
COMMENT                             #158=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #87# 12341 100) 
COMMENT                                   (LOGPEN 0 0 8421376) 1 
COMMENT                                   (LOGFONT 41 15 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 0 
COMMENT                                   "{\\i Bal} I (6003)" "@N (@S)" 5 -13.176 
COMMENT                                   -6.81673 0 -10 4.23077 1.02564 #157#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #159=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #5# 0) 1 2 0 0 Nil -1061 100) 
COMMENT                            (CObjectList 
COMMENT                             #160=(CScratch 
COMMENT                                   (CWidget 1 (1 #5# 1) 1 2 0 0 #109# 431 100) 
COMMENT                                   (LOGPEN 0 6 0) 8 1 1.5678 1.9 0.082322 1) 
COMMENT                             #161=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #87# 6116508 100) 
COMMENT                                   (LOGPEN 0 0 8421376) 1 
COMMENT                                   (LOGFONT 41 15 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 0 
COMMENT                                   "{\\i Pst} I (7141)" "@N (@S)" 5 -13.6323 
COMMENT                                   0.996221 0 -10 4.12821 1.02564 #160#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #162=(CGroupWidget 
COMMENT                            (CWidget 2 (10 #0# 0) 1 2 0 0 Nil -381 100) 
COMMENT                            (CObjectList 
COMMENT                             #163=(CScratch 
COMMENT                                   (CWidget 1 (1 #0# 1) 1 2 0 0 #109# 431 100) 
COMMENT                                   (LOGPEN 0 6 0) 8 1 3.67007 1.9 0.082322 1) 
COMMENT                             #164=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #91# 10289784 100) 
COMMENT                                   (LOGPEN 0 0 8421376) 1 
COMMENT                                   (LOGFONT 41 15 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 0 
COMMENT                                   "{\\i Eco}R I (3951)" "@N (@S)" 5 8.75103 
COMMENT                                   -11.095 0 -10 4.82051 1.02564 #163#) 
COMMENT                             #165=(CScratch 
COMMENT                                   (CWidget 2 (1 #0# 2) 1 2 0 0 #109# 431 100) 
COMMENT                                   (LOGPEN 0 6 0) 8 1 2.35533 1.9 0.082322 1) 
COMMENT                             #166=(CLabel (CWidget 0 (0 0) 1 2 0 0 #91# 0 100) 
COMMENT                                   (LOGPEN 0 0 8421376) 1 
COMMENT                                   (LOGFONT 41 15 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 0 
COMMENT                                   "{\\i Eco}R I (5946)" "@N (@S)" 5 -11.342 
COMMENT                                   -10.3771 0 -10 4.82051 1.02564 #165#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #167=(CGroupWidget 
COMMENT                            (CWidget 2 (10 #1# 0) 1 2 0 0 Nil -246 100) 
COMMENT                            (CObjectList 
COMMENT                             #168=(CScratch 
COMMENT                                   (CWidget 1 (1 #1# 1) 1 2 0 0 #109# 419 100) 
COMMENT                                   (LOGPEN 0 6 0) 8 1 2.31315 1.9 0.082322 1) 
COMMENT                             #169=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #91# 1885431891 100) 
COMMENT                                   (LOGPEN 0 0 8421376) 1 
COMMENT                                   (LOGFONT 41 15 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 0 
COMMENT                                   "{\\i Nco} I (6010)" "@N (@S)" 5 -13.0151 
COMMENT                                   -5.81799 0 -10 4.38462 1.02564 #168#) 
COMMENT                             #170=(CScratch 
COMMENT                                   (CWidget 2 (1 #1# 2) 1 2 0 0 #109# 6116508 
COMMENT                                    100) (LOGPEN 0 6 0) 8 1 2.14642 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #171=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #91# -22418 100) 
COMMENT                                   (LOGPEN 0 0 8421376) 1 
COMMENT                                   (LOGFONT 41 15 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 0 
COMMENT                                   "{\\i Nco} I (6263)" "@N (@S)" 5 -13.0772 
COMMENT                                   -4.79361 0 -10 4.38462 1.02564 #170#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #172=(CGroupWidget 
COMMENT                            (CWidget 2 (10 #4# 0) 1 2 0 0 Nil -110 100) 
COMMENT                            (CObjectList 
COMMENT                             #173=(CScratch 
COMMENT                                   (CWidget 1 (1 #4# 1) 1 2 0 0 #109# 419 100) 
COMMENT                                   (LOGPEN 0 6 0) 8 1 2.3316 1.9 0.082322 1) 
COMMENT                             #174=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #91# 13873 100) 
COMMENT                                   (LOGPEN 0 0 8421376) 1 
COMMENT                                   (LOGFONT 41 15 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 0 
COMMENT                                   "{\\i Nhe} I (5982)" "@N (@S)" 5 -13.1335 
COMMENT                                   -7.91804 0 -10 4.38462 1.02564 #173#) 
COMMENT                             #175=(CScratch 
COMMENT                                   (CWidget 2 (1 #4# 2) 1 2 0 0 #109# 431 100) 
COMMENT                                   (LOGPEN 0 6 0) 8 1 1.81362 1.9 0.082322 1) 
COMMENT                             #176=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #91# -22152 100) 
COMMENT                                   (LOGPEN 0 0 8421376) 1 
COMMENT                                   (LOGFONT 41 15 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 0 
COMMENT                                   "{\\i Nhe} I (6768)" "@N (@S)" 5 -13.6413 
COMMENT                                   -1.53972 0 -10 4.38462 1.02564 #175#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #177=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -858 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #178=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -993 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 6 (CObjectList) (CObList) 1 (CObList)) (CObList) 1196705607 
COMMENT       (CStringList) 1094795585 1195852099 (CObList)))
FEATURES             Location/Qualifiers
     rep_origin      complement(2..982)
                     /vntifkey="33"
                     /label=2mu\ori
     rep_origin      complement(2661..3116)
                     /vntifkey="33"
                     /label=f1\ori
     promoter        5075..5478
                     /vntifkey="30"
                     /label=ADH1\promoter
     CDS             5501..5941
                     /vntifkey="4"
                     /label=GALbd
     misc_difference 5957..5983
                     /vntifkey="85"
                     /label=HA\epitope
     CDS             6011..7129
                     /vntifkey="4"
                     /label=mCASP-11m
     terminator      7157..7258
                     /vntifkey="43"
                     /label=ADH1\terminator
     rep_origin      complement(7586..7988)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(8529..9389)
                     /vntifkey="4"
                     /label=AMP
     CDS             1884..2558
                     /vntifkey="4"
                     /label=TRP1
     CDS             complement(3855..4304)
                     /vntifkey="4"
                     /label=CYH2
BASE COUNT     2713 a      2001 c      2119 g      2686 t 
ORIGIN
        1 gaacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt 
       61 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt 
      121 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta 
      181 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg 
      241 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag 
      301 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat 
      361 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg 
      421 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact 
      481 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga 
      541 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca 
      601 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac 
      661 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa 
      721 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag 
      781 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag 
      841 atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt 
      901 ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg 
      961 ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt 
     1021 tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca 
     1081 cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt 
     1141 gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc 
     1201 tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact 
     1261 accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc 
     1321 tatcatttcc tttgatattg gatcatatta agaaaccatt attatcatga cattaaccta 
     1381 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa 
     1441 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 
     1501 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta 
     1561 tgcggcatca gagcagattg tactgagagt gcaccataga tcaacgacat tactatatat 
     1621 ataatatagg aagcatttaa tagacagcat cgtaatatat gtgtactttg cagttatgac 
     1681 gccagatggc agtagtggaa gatattcttt attgaaaaat agcttgtcac cttacgtaca 
     1741 atcttgatcc ggagcttttc tttttttgcc gattaagaat taattcggtc gaaaaaagaa 
     1801 aaggagaggg ccaagaggga gggcattggt gactattgag cacgtgagta tacgtgatta 
     1861 agcacacaaa ggcagcttgg agtatgtctg ttattaattt cacaggtagt tctggtccat 
     1921 tggtgaaagt ttgcggcttg cagagcacag aggccgcaga atgtgctcta gattccgatg 
     1981 ctgacttgct gggtattata tgtgtgccca atagaaagag aacaattgac ccggttattg 
     2041 caaggaaaat ttcaagtctt gtaaaagcat ataaaaatag ttcaggcact ccgaaatact 
     2101 tggttggcgt gtttcgtaat caacctaagg aggatgtttt ggctctggtc aatgattacg 
     2161 gcattgatat cgtccaactg catggagatg agtcgtggca agaataccaa gagttcctcg 
     2221 gtttgccagt tattaaaaga ctcgtatttc caaaagactg caacatacta ctcagtgcag 
     2281 cttcacagaa acctcattcg tttattccct tgtttgattc agaagcaggt gggacaggtg 
     2341 aacttttgga ttggaactcg atttctgact gggttggaag gcaagagagc cccgaaagct 
     2401 tacattttat gttagctggt ggactgacgc cagaaaatgt tggtgatgcg cttagattaa 
     2461 atggcgttat tggtgttgat gtaagcggag gtgtggagac aaatggtgta aaagactcta 
     2521 acaaaatagc aaatttcgtc aaaaatgcta agaaataggt tattactgag tagtatttat 
     2581 ttaagtattg tttgtgcact tgccgatcta tgcggtgtga aataccgcac agatgcgtaa 
     2641 ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa 
     2701 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa 
     2761 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact 
     2821 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc 
     2881 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa 
     2941 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc 
     3001 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt 
     3061 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcgcg 
     3121 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 
     3181 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 
     3241 gttttcccag tcacgacgtt gtaaaacgac ggccagtcgt ccaagctttc gcgagctcga 
     3301 gatcccgagc tttgcaaatt aaagccttcg agcgtcccaa aaccttctca agcaaggttt 
     3361 tcagtataat gttacatgcg tacacgcgtc tgtacagaaa aaaaagaaaa atttgaaata 
     3421 taaataacgt tcttaatact aacataacta taaaaaaata aatagggacc tagacttcag 
     3481 gttgtctaac tccttccttt tcggttagag cggatgtggg gggagggcgt gaatgtaagc 
     3541 gtgacataac taattacatg atatcgacaa aggaaaaggg gcctgtttac tcacaggctt 
     3601 ttttcaagta ggtaattaag tcgtttctgt ctttttcctt cttcaaccca ccaaaggcca 
     3661 tcttggtact tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt 
     3721 tttttttttt tttttttttt tttttttttt tttttttttt tttcatagaa ataatacaga 
     3781 agtagatgtt gaattagatt aaactgaaga tatataattt attggaaaat acatagagct 
     3841 ttttgttgat gcgcttaagc gatcaattca acaacaccac cagcagctct gattttttct 
     3901 tcagccaact tggagacgaa tctagctttg acgataactg gaacatttgg aattctaccc 
     3961 ttacccaaga tcttaccgta accggctgcc aaagtgtcaa taactggagc agtttcctta 
     4021 gaagcagatt tcaagtattg gtctctcttg tcttctggga tcaatgtcca caatttgtcc 
     4081 aagttcaaga ctggcttcca gaaatgagct tgttgcttgt ggaagtatct cataccaacc 
     4141 ttaccgaaat aacctggatg gtatttatcc atgttaattc tgtggtgatg ttgaccaccg 
     4201 gccatacctc taccaccggg gtgctttctg tgcttaccga tacgaccttt accggctgag 
     4261 acgtgacctc tgtgctttct agtcttagtg aatctggaag gcattcttga ttagttggat 
     4321 gattgttctg ggatttaatg caaaaaaatc actaagaagg aaaaaaatca acggagaaag 
     4381 caaacgccat cttaaatata cgggatacag atgaaaggtt tgaacctatc tgggaaaata 
     4441 cgcattaaac aagcgaaaaa ctgcgaggaa aattgtttgc gtctctgcgg gctattcacg 
     4501 cgccagagga aaataggaaa aataacaggg cattagaaaa ataattttga ttttggtaat 
     4561 gtgtgggtcc ctggtgtaca gatgttacat tggttacagt actcttgttt ttgctgtgtt 
     4621 tttcgatgaa tctccaaaat ggttgttagc acatggaaga gtcaccgatg ctaagttatc 
     4681 tctatgtaag ctacgtggcg tgacttttga tgaagccgca caagagatac aggattggca 
     4741 actgcaaata gaatctgggg atctagatat ccttttgttg tttccgggtg tacaatatgg 
     4801 acttcctctt ttctggcaac caaacccata catcgggatt cctataatac cttcgttggt 
     4861 ctccctaaca tgtaggtggc ggaggggaga tatacaatag aacagatacc agacaagaca 
     4921 taatgggcta aacaagacta caccaattac actgcctcat tgatggtggt acataacgaa 
     4981 ctaatactgt agccctagac ttgatagcca tcatcatatc gaagtttcac tacccttttt 
     5041 ccatttgcca tctattgaag taataatagg cgcatgcaac ttcttttctt tttttttctt 
     5101 ttctctctcc cccgttgttg tctcaccata tccgcaatga caaaaaaaat gatggaagac 
     5161 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga 
     5221 tgaggggtat cttcgaacac acgaaacttt ttccttcctt cattcacgca cactactctc 
     5281 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaagttt 
     5341 gccgctttgc tatcaagtat aaatagacct gcaattatta atcttttgtt tcctcgtcat 
     5401 tgttctcgtt ccctttcttc cttgtttctt tttctgcaca atatttcaag ctataccaag 
     5461 catacaatca actccaagct tgaagcaagc ctcctgaaag atgaagctac tgtcttctat 
     5521 cgaacaagca tgcgatattt gccgacttaa aaagctcaag tgctccaaag aaaaaccgaa 
     5581 gtgcgccaag tgtctgaaga acaactggga gtgtcgctac tctcccaaaa ccaaaaggtc 
     5641 tccgctgact agggcacatc tgacagaagt ggaatcaagg ctagaaagac tggaacagct 
     5701 atttctactg atttttcctc gagaagacct tgacatgatt ttgaaaatgg attctttaca 
     5761 ggatataaaa gcattgttaa caggattatt tgtacaagat aatgtgaata aagatgccgt 
     5821 cacagataga ttggcttcag tggagactga tatgcctcta acattgagac agcatagaat 
     5881 aagtgcgaca tcatcatcgg aagagagtag taacaaaggt caaagacagt tgactgtatc 
     5941 gccggaattc atggcttacc catacgatgt tccagattac gctagcttgg gtggtcatat 
     6001 ggccaccacc atggctgaaa acaaacaccc tgacaaacca cttaaggtgt tggaacagct 
     6061 gggcaaagaa gtccttacgg agtacctaga aaaattagta caaagcaatg tactgaaatt 
     6121 aaaggaggaa gataaacaaa aatttaacaa tgctgaacgc agtgacaagc gttgggtttt 
     6181 tgtagatgcc atgaaaaaga aacacagcaa agtaggtgaa atgcttctcc agacattctt 
     6241 cagtgtggac ccaggcagcc accatggtga agctaatctg gaaatggagg aaccagaaga 
     6301 atcattgaac actctcaagc tttgttcccc tgaagagttc acaaggcttt gcagagaaaa 
     6361 gacacaagaa atttacccaa taaaggaggc caatggccgt acacgaaagg ctcttatcat 
     6421 atgcaataca gagttcaaac atctctcact gaggtatggg gctaactttg acatcattgg 
     6481 tatgaaaggc cttcttgaag acttaggcta cgatgtggtg gtgaaagagg agcttacagc 
     6541 agagggcatg gagtcagaga tgaaagactt tgctgcactc tcagaacacc agacatcaga 
     6601 cagcacattc ctggtgctaa tgtctcatgg cacactgcat ggcatttgtg gaacaatgca 
     6661 cagtgaaaaa actccagatg tgctacagta tgataccatc tatcagatat tcaacaattg 
     6721 ccactgtcca ggtctacgag acaaacccaa agtcatcatt gtgcaggcta gcagaggtgg 
     6781 gaactctgga gaaatgtgga tcagagagtc ttcaaaaccc cagttgtgca gaggtgtaga 
     6841 tctacctagg aatatggaag ctgatgctgt caagctgagc cacgtggaga aggacttcat 
     6901 tgccttctac tctacaaccc cacatcactt gtcctaccga gacaaaacag gaggctctta 
     6961 cttcatcact agactcattt cctgcttccg gaaacatgct tgctcttgtc atctctttga 
     7021 tatattcctg aaggtgcaac aatcatttga aaaggcaagt attcattccc agatgcccac 
     7081 cattgatcgg gcaaccttga cgagatattt ctacttcttt cctggcaacg tcgacctgca 
     7141 gccaagctaa ttccgggcga atttcttatg atttatgatt tttattatta aataagttat 
     7201 aaaaaaaata agtgtataca aattttaaag tgactcttag gttttaaaac gaaaattctt 
     7261 gttcttgagt aactctttcc tgtaggtcag gttgctttct caggtatagc atgaggtcgc 
     7321 tcttattgac cacacctcta ccggcatgca agcttggcgt aatcatggtc atagctgttt 
     7381 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag 
     7441 tgtaaagcct ggggtgccta atgagtgagg taactcacat taattgcgtt gcgctcactg 
     7501 cccgctttcc agtcgggaaa cctgtcgtgc cagctggatt aatgaatcgg ccaacgcgcg 
     7561 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc 
     7621 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 
     7681 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 
     7741 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 
     7801 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 
     7861 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 
     7921 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 
     7981 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 
     8041 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 
     8101 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 
     8161 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt 
     8221 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 
     8281 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 
     8341 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 
     8401 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 
     8461 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 
     8521 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 
     8581 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 
     8641 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 
     8701 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 
     8761 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 
     8821 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 
     8881 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 
     8941 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 
     9001 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 
     9061 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 
     9121 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 
     9181 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 
     9241 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 
     9301 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 
     9361 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 
     9421 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 
     9481 ataggggttc cgcgcacatt tccccgaaaa gtgccacct 
//
