LOCUS pBlue-mA20f3 4056 bp DNA circular 19-MAR-1980 DEFINITION LMBP 3952. SOURCE ORGANISM COMMENT (800)357-3114 or http://www.informaxinc.com/ COMMENT VNTNAME|pBlue-mA20f3| COMMENT VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection| COMMENT VNTOAUTHORTEL|0032-9-33-13-843| COMMENT VNTOAUTHOREML|bccm.genecorner@UGent.be| COMMENT VNTOAUTHORWWW|www.genecorner.ugent.be| COMMENT VNTOAUTHORAD1|Ghent University| COMMENT VNTOAUTHORAD2|Technologiepark 927| COMMENT VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| COMMENT VNTREPLTYPE|Plasmid COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) COMMENT (SXF COMMENT (CGexDoc "pBlue-mA20f3" 0 4056 COMMENT (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) COMMENT (CObList) -1 "") COMMENT (CDocSetData 1 1 0 0 0 0 " all enzymes of LMBP" 1 1 1 1 0 0 1 1 0 1 10 6 COMMENT 4000 50 0 1 0 (CHomObj 0 0 0 3 75) COMMENT (CWordArray 0 4 15 20 21 22 29 30 33 43) (CWordArray) COMMENT (CStringList "BamHI" "EcoRI" "KpnI" "SalI" "XbaI" "XhoI") COMMENT (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 COMMENT "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 COMMENT (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) COMMENT (CMolPar 0 0 0 0 0 1 4056 0 0 0 0 0 0 0 0) (CStringList) (CStringList) COMMENT (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) COMMENT (COAPar 25 250 50 0 6 4 3 7) COMMENT (CObList #0=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 0 0 "") COMMENT #1=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 712 0 0 "") COMMENT #2=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 1855 0 0 "") COMMENT #3=(CRSite (CStringList) "SalI" "gtcgac" 2 0 1 739 0 0 "") COMMENT #4=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 678 0 0 "") COMMENT #5=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 0 0 "")) COMMENT (CObList COMMENT #6=(CFSignal (CObList) "T7 promoter" 30 0 0 626 642 0 (CStringList) COMMENT (CStringList) 1 1 0 0 "") COMMENT #7=(CFSignal (CObList) "f1 ori" 33 0 0 4 459 0 (CStringList) COMMENT (CStringList) 1 1 0 0 "") COMMENT #8=(CFSignal (CObList) "AMP" 4 0 1 3068 3928 0 (CStringList) COMMENT (CStringList) 1 1 0 0 "") COMMENT #9=(CFSignal (CObList) "lacZ RBS" 32 0 1 1912 1949 0 (CStringList) COMMENT (CStringList) 1 1 0 0 "") COMMENT #10=(CFSignal (CObList) "lac promoter" 30 0 1 1950 2033 0 (CStringList) COMMENT (CStringList) 1 1 0 0 "") COMMENT #11=(CFSignal (CObList) "pMB1 ori" 33 0 1 2125 2527 0 (CStringList) COMMENT (CStringList) 1 1 0 0 "") COMMENT #12=(CFSignal (CObList) "T3 promoter" 30 0 1 1870 1886 0 (CStringList) COMMENT (CStringList) 1 1 0 0 "") COMMENT #13=(CFSignal (CObList) "mA20 (1-366)" 4 0 1 746 1846 0 (CStringList) COMMENT (CStringList) 1 1 0 0 "")) (CObList) (CObList) (CObList) (CObList) COMMENT (CObList) (CObList) COMMENT (CTextView 0 COMMENT #14=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) COMMENT (CObjectList COMMENT #15=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 2 0 1 178) COMMENT (CObjectList COMMENT #16=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "DNA Plasmid pBlue-mA20f3" 0) COMMENT #17=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 3952" COMMENT 0) COMMENT #18=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "length: 4056 bp" 0) COMMENT #19=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "storage type: Basic" 0) COMMENT #20=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "form: Circular" 0))) "General Description") COMMENT #21=(CFolderPar COMMENT (CGroupPar (CParagraph 10 (6 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #22=(CFolderPar COMMENT (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #23=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 746 (3 #13# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #24=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 746 End: 1846 (Complementary)" COMMENT 0) COMMENT #25=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Mouse zinc finger protein A20 cDNA (mA20) fragment, containing codons 1 to 366." 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0 178) COMMENT (CObjectList COMMENT #44=(CFolderPar COMMENT (CGroupPar (CParagraph 4 (3 #7# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #45=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 4 End: 459 " 0) COMMENT #46=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) "" 0))) COMMENT "f1 ori") COMMENT #47=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2125 (3 #11# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #48=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 2125 End: 2527 (Complementary)" COMMENT 0) COMMENT #49=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) "" 0))) COMMENT "pMB1 ori"))) "Rep_origin (2 signals)"))) COMMENT "Functional Map") COMMENT #50=(CFolderPar COMMENT (CGroupPar (CParagraph 11 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #51=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 11298556 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) "BamHI: 0 sites") 1 5 "GGATCC" COMMENT "CCTAGG") COMMENT #52=(CRSFolderPar 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3.69231 1.02564 #133#)) (CObjectList)) COMMENT #135=(CGroupWidget (CWidget 1 (10 #4# 0) 1 2 0 0 Nil 0 100) COMMENT (CObjectList COMMENT #136=(CScratch COMMENT (CWidget 1 (1 #4# 1) 1 2 0 0 #89# 178 100) COMMENT (LOGPEN 0 6 0) 8 1 5.22611 1.9 0.082322 1) COMMENT #137=(CLabel (CWidget 0 (0 0) 1 2 0 0 #67# 4 100) COMMENT (LOGPEN 0 0 8421376) 1 COMMENT (LOGFONT 41 15 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Times New Roman") 1.74233 0.611111 0 COMMENT "{\\i Xba} I (678)" "@N (@S)" 5 13.6826 COMMENT 6.20135 0 -10 3.94872 1.02564 #136#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #138=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -449 100) COMMENT (CObjectList) (CObjectList)) COMMENT #139=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil 0 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 6 (CObjectList) (CObList) 1 (CObList)) (CObList) 1196705607 COMMENT (CStringList) 1094795585 1195852099 (CObList))) FEATURES Location/Qualifiers promoter 626..642 /vntifkey="30" /label=T7\promoter rep_origin 4..459 /vntifkey="33" /label=f1\ori CDS complement(3068..3928) /vntifkey="4" /label=AMP RBS complement(1912..1949) /vntifkey="32" /label=lacZ\RBS promoter complement(1950..2033) /vntifkey="30" /label=lac\promoter rep_origin complement(2125..2527) /vntifkey="33" /label=pMB1\ori promoter complement(1870..1886) /vntifkey="30" /label=T3\promoter CDS complement(746..1846) /vntifkey="4" /label=mA20\(1-366) /note="Mouse zinc finger protein A20 cDNA (mA20) fragment, containing codons 1 to 366." BASE COUNT 966 a 1021 c 983 g 1086 t ORIGIN 1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga 61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg 121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat 181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg 241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt 361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat 421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg 481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca 661 ccgcggtggc ggccgctcta gaactagtgg atcgatcccc cgggctgcag gaattcgata 721 tcaagcttat cgataccgtc gacacttacg ctctcctggc ctgatcgctg ttctcctgcc 781 atttcttgta ttcgtgctga acaagctcaa agtaatcgtc taccaaattt atttctttgg 841 gtaagttagc ttcatccaat tttgcagcgt tgatcaggtg agtcgtgccg tggtcccagc 901 cttgcacagg gatctccatc actatcaagt actcctttag aagcttttcc ttcatctcat 961 tctcaggatc tgtcaagaag tgaactttta agtcttcaaa cctaccccgg tctctgttaa 1021 caagtggaac agcgcgaagt tcaggtccac tgtccttcag ggtcaccagg ggtacaaagt 1081 gctggctgtc atagcctagg acgatgggat atctgtaaca ctcctgggca ggccagtgaa 1141 gaggcagata aatcccaccc actttcaaag gagcaaaatt ggaaccagat tccaaacttc 1201 ttagcatttt gtctgaaatg acaatgatgg gtcttctgag gatgttgctg aggacaaata 1261 tgtggatttc ttccagggaa ttgtactgaa gtccacttcg ggctgcaggt gtgtctgctg 1321 atgccatttt gaccaagttg tcccattcgt cattccagtt ccgagtgtcg tagcaaagtc 1381 ctgtttccac aaattcctga gatttcagag attccagctg ccagcggaat ttaaagttcc 1441 gagtgtctgt ctccttaagg gtgctgcaga gggccttcct caggaccagg tcagtatcct 1501 gaacacccca catgtactga caagctgcat gcatgaggca gtttccatca ccattggttt 1561 tcagagccac gagcttcctg acttcacgac accagttgag cttcttctgg ctttccaggg 1621 aagcctggac acttctgtca ataagtgctt tgtggatgat ctctcggaac tgtgggcaaa 1681 actggcatgt tctgaacatc tccagcgtgt atcggtgcat ggttttaaag tgatagatga 1741 tcccattggt aggtttgaaa atgtcttctg gggttctctc tcgtatcttc acagctttcc 1801 gcatattgct caaatacaaa gcctgaggaa gaagttgttc agccatggcg gtacccagct 1861 tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc 1921 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt 1981 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc 2041 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg 2101 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct 2161 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 2221 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 2281 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 2341 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 2401 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 2461 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 2521 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 2581 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 2641 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 2701 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg 2761 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 2821 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 2881 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 2941 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 3001 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 3061 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 3121 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 3181 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 3241 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 3301 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 3361 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 3421 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 3481 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 3541 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 3601 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 3661 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 3721 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 3781 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 3841 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 3901 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 3961 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 4021 taggggttcc gcgcacattt ccccgaaaag tgccac //