ID pCAGGS-FLAGmA20f3 standard; circular DNA; ; 5872 BP. DE LMBP 3953. CC Accession number: LMBP 3953 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 09-JUN-2009 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT misc_marker 13..36 FT /vntifkey="22" FT /label=FLAG\epitope FT promoter complement(1684..1766) FT /vntifkey="30" FT /label=lac\promoter FT terminator 1158..1249 FT /vntifkey="43" FT /label=3'\UTR\of\ß-globin,\incl.\polyA FT promoter 4145..4522 FT /vntifkey="29" FT /label=hCMV-IE\enhancer FT CDS complement(3145..4005) FT /vntifkey="4" FT /label=ampicillin\resistance FT rep_origin 1908..1983 FT /vntifkey="33" FT /label=SV40\ori FT rep_origin complement(2203..2604) FT /vntifkey="33" FT /label=pMB1\ori FT intron 4897..5821 FT /vntifkey="15" FT /label=intron\AG\promoter FT CDS 46..1143 FT /vntifkey="4" FT /label=mA20\(1-366) FT misc_feature 1250..1606 FT /vntifkey="21" FT /label=3'\flanking\region\of\rabbit\ß-globin FT terminator 2014..2088 FT /vntifkey="43" FT /label=SV40\polyA\early FT terminator complement(2014..2088) FT /vntifkey="43" FT /label=SV40\polyA\late FT promoter 4528..5869 FT /vntifkey="29" FT /label=AG\promoter SQ Sequence 5872 BP; 1325 A; 1515 C; 1595 G; 1437 t; ttcctcgaga tggactacaa ggacgacgat gacaagggta ccgccatggc tgaacaactt 60 cttcctcagg ctttgtattt gagcaatatg cggaaagctg tgaagatacg agagagaacc 120 ccagaagaca ttttcaaacc taccaatggg atcatctatc actttaaaac catgcaccga 180 tacacgctgg agatgttcag aacatgccag ttttgcccac agttccgaga gatcatccac 240 aaagcactta ttgacagaag tgtccaggct tccctggaaa gccagaagaa gctcaactgg 300 tgtcgtgaag tcaggaagct cgtggctctg aaaaccaatg gtgatggaaa ctgcctcatg 360 catgcagctt gtcagtacat gtggggtgtt caggatactg acctggtcct gaggaaggcc 420 ctctgcagca cccttaagga gacagacact cggaacttta aattccgctg gcagctggaa 480 tctctgaaat ctcaggaatt tgtggaaaca ggactttgct acgacactcg gaactggaat 540 gacgaatggg acaacttggt caaaatggca tcagcagaca cacctgcagc ccgaagtgga 600 cttcagtaca attccctgga agaaatccac atatttgtcc tcagcaacat cctcagaaga 660 cccatcattg tcatttcaga caaaatgcta agaagtttgg aatctggttc caattttgct 720 cctttgaaag tgggtgggat ttatctgcct cttcactggc ctgcccagga gtgttacaga 780 tatcccatcg tcctaggcta tgacagccag cactttgtac ccctggtgac cctgaaggac 840 agtggacctg aacttcgcgc tgttccactt gttaacagag accggggtag gtttgaagac 900 ttaaaagttc acttcttgac agatcctgag aatgagatga aggaaaagct tctaaaggag 960 tacttgatag tgatggagat ccctgtgcaa ggctgggacc acggcacgac tcacctgatc 1020 aacgctgcaa aattggatga agctaactta cccaaagaaa taaatttggt agacgattac 1080 tttgagcttg ttcagcacga atacaagaaa tggcaggaga acagcgatca ggccaggaga 1140 gcgtaagtgt cgacgaagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc 1200 cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt 1260 ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc 1320 agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct gccatgaaca 1380 aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc cattccttat 1440 tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt gtgttatttt 1500 tttctttaac atccctaaaa ttttccttac atgttttact agccagattt ttcctcctct 1560 cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc gacctgcagc 1620 ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca 1680 attccacaca acatacgagc cggaagcata aagtgtaaag cctgggtgcc taatgagtga 1740 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 1800 gccagcggat ccgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat 1860 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt 1920 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg 1980 cttttttgga ggcctaggct tttgcaaaaa gctaacttgt ttattgcagc ttataatggt 2040 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 2100 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccg ctgcattaat 2160 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc 2220 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 2280 cggtaatacg gttatccaca gaatcaggga taacgcagga aagaacatgt gagcaaaagg 2340 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 2400 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 2460 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 2520 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 2580 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 2640 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 2700 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 2760 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 2820 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 2880 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 2940 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 3000 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 3060 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 3120 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 3180 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 3240 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 3300 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 3360 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 3420 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 3480 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 3540 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 3600 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 3660 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 3720 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 3780 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 3840 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 3900 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 3960 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 4020 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 4080 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgggtc 4140 gatcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat 4200 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg 4260 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata 4320 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta 4380 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc 4440 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 4500 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc 4560 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca 4620 gcgatggggg cggggggggg gggggggccc cccccaggcg gggcggggcg gggcgagggg 4680 cggggcgggg cgaggcggaa aggtgcggcg gcagccaatc agagcggcgc gctccgaaag 4740 tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg 4800 gcgggagtcg ttgcgcgctg ccttcccccc gtgccccgct ccgccgccgc ctcgcgccgc 4860 ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct 4920 cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt 4980 gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg 5040 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag 5100 cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc 5160 gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt 5220 gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg 5280 cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg 5340 cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg 5400 gcggggccgc ctcgggccgg ggagggctcg ggggaagggg cgcggcggcc cccggagcgc 5460 cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag 5520 ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg 5580 caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg 5640 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg 5700 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg 5760 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca 5820 gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aa 5872 //