ID pCAGGS-FLAGmA20 standard; circular DNA; ; 7099 BP. DE LMBP 3954. CC Accession number: LMBP 3954 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium). CC Last modified: 24-OCT-2007 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT misc_marker 13..36 FT /vntifkey="22" FT /label=FLAG\epitope FT /note="FLAG epitope tag" FT CDS 46..2373 FT /vntifkey="4" FT /label=mA20 FT /note="mouse zinc finger protein A20 cDNA coding sequence" FT terminator 2385..2476 FT /vntifkey="43" FT /label=ß-globin\polyA FT /note="3' UTR of the rabbit ß-globin gene, incl. polyA" FT misc_feature 2477..2833 FT /vntifkey="21" FT /label=3'\FR\of\ß-globin FT /note="3' flanking region of the rabbit ß-globin gene" FT promoter complement(2911..2993) FT /vntifkey="30" FT /label=lac\promoter FT /note="E. coli lac operon promoter" FT rep_origin 3135..3210 FT /vntifkey="33" FT /label=SV40\ori FT /note="bidirectional origin of SV40" FT terminator 3241..3315 FT /vntifkey="43" FT /label=SV40\polyA\early FT /note="polyA signal of the SV40 early region" FT terminator complement(3241..3315) FT /vntifkey="43" FT /label=SV40\polyA\late FT /note="polyA signal of the SV40 late region" FT rep_origin complement(3430..3831) FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT CDS complement(4372..5232) FT /vntifkey="4" FT /label=ampicillin FT /note="antibiotic resistance gene; coding sequence" FT promoter 5372..5749 FT /vntifkey="29" FT /label=hCMV-IE\enhancer FT /note="human CMV-IE enhancer" FT promoter 5755..7096 FT /vntifkey="29" FT /label=AG\promoter FT /note="chicken ß-actin/rabbit ß-globin hybrid promoter" FT intron 6124..7048 FT /vntifkey="15" FT /label=intron\AG\promoter FT /note="chicken ß-actin/rabbit ß-globin hybrid intron" SQ Sequence 7099 BP; 1637 A; 1881 C; 1894 G; 1687 t; ttcctcgaga tggactacaa ggacgacgat gacaagggta ccgccatggc tgaacaactt 60 cttcctcagg ctttgtattt gagcaatatg cggaaagctg tgaagatacg agagagaacc 120 ccagaagaca ttttcaaacc taccaatggg atcatctatc actttaaaac catgcaccga 180 tacacgctgg agatgttcag aacatgccag ttttgcccac agttccgaga gatcatccac 240 aaagcactta ttgacagaag tgtccaggct tccctggaaa gccagaagaa gctcaactgg 300 tgtcgtgaag tcaggaagct cgtggctctg aaaaccaatg gtgatggaaa ctgcctcatg 360 catgcagctt gtcagtacat gtggggtgtt caggatactg acctggtcct gaggaaggcc 420 ctctgcagca cccttaagga gacagacact cggaacttta aattccgctg gcagctggaa 480 tctctgaaat ctcaggaatt tgtggaaaca ggactttgct acgacactcg gaactggaat 540 gacgaatggg acaacttggt caaaatggca tcagcagaca cacctgcagc ccgaagtgga 600 cttcagtaca attccctgga agaaatccac atatttgtcc tcagcaacat cctcagaaga 660 cccatcattg tcatttcaga caaaatgcta agaagtttgg aatctggttc caattttgct 720 cctttgaaag tgggtgggat ttatctgcct cttcactggc ctgcccagga gtgttacaga 780 tatcccatcg tcctaggcta tgacagccag cactttgtac ccctggtgac cctgaaggac 840 agtggacctg aacttcgcgc tgttccactt gttaacagag accggggtag gtttgaagac 900 ttaaaagttc acttcttgac agatcctgag aatgagatga aggaaaagct tctaaaggag 960 tacttgatag tgatggagat ccctgtgcaa ggctgggacc acggcacgac tcacctgatc 1020 aacgctgcaa aattggatga agctaactta cccaaagaaa taaatttggt agacgattac 1080 tttgagcttg ttcagcacga atacaagaaa tggcaggaga acagcgatca ggccaggaga 1140 gcggcacatg cgcagaaccc cttggagcct tccacacccc agctatcact catggatata 1200 aaatgtgaga cacccaactg tcctttcttc atgtccgtga acactcagcc tttatgccac 1260 gaatgctcag agaggcgcca aaagaatcag agcaagctcc caaagctgaa ctcgaagcta 1320 ggccctgaag gactcccagg cgtgggactt ggctcctcaa actggagccc cgaggaaacc 1380 gctggaggac ctcattcagc cccacccaca gcacccagcc tttttctctt cagtgagacc 1440 actgcaatga agtgcaggag tcctgggtgc ccttttactt tgaatgtgca gcataatgga 1500 ttctgtgagc gttgccacgc ccggcagatt aatgccagcc acaccgcaga ccctggaaag 1560 tgccaagcct gccttcagga tgtcactcgg acctttaatg gcatctgcag tacctgtttc 1620 aaaaggacta cagcagagcc cagctccagc ctcacttcca gtatccctgc ctcctgtcac 1680 caacgctcca agtctgaccc ctcacaactc atccaaagtc tcactccaca ctcttgccac 1740 cggactggaa atgtctctcc ttctggctgc ctctcccagg ctgcacggac tccaggagac 1800 agagcaggga caagcaagtg caggaaagct ggctgcatgt attttgggac tccagaaaac 1860 aagggctttt gcactctatg tttcatcgaa tacagagaaa ataagcagtc tgttactgcc 1920 tctgcgaaag ctggttcccc ggcccccagg ttccagaaca atgtcccgtg cctgggcagg 1980 gagtgcggca cactcggaag caccatgttt gaagggtact gtcagaagtg tttcatcgaa 2040 gctcagaacc agagattcca tgaagcaaga agaacggaag aacagctgag atcaagccag 2100 catagagaca tgcctcgaac tacacaggta gcctcaaggc cgaaatgtgc ccgggcctcc 2160 tgcaagaaca ttctggcctg tcgcagtgag gaactctgta tggagtgcca gcacctaagc 2220 caacgagtag gttctgtggc ccaccggggt gagcccacgc ctgaagagcc ccctaaacag 2280 cgctgccggg cccctgcttg tgatcacttt ggcaatgcca agtgtaatgg ttactgcaat 2340 gagtgctacc agttcaagca gatgtatggc taagtgtcga cgaagatctt tttccctctg 2400 ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga 2460 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat 2520 atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat 2580 atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga 2640 aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt 2700 tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg 2760 ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc 2820 tcttatggag atccctcgac ctgcagccca agcttggcgt aatcatggtc atagctgttt 2880 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag 2940 tgtaaagcct gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc 3000 ccgctttcca gtcgggaaac ctgtcgtgcc agcggatccg catctcaatt agtcagcaac 3060 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc 3120 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc 3180 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct 3240 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 3300 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 3360 tatcatgtct ggatccgctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 3420 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 3480 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcagggataa 3540 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 3600 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 3660 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 3720 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 3780 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 3840 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 3900 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 3960 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 4020 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct 4080 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 4140 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 4200 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 4260 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 4320 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 4380 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 4440 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 4500 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 4560 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 4620 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 4680 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 4740 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 4800 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 4860 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 4920 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 4980 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 5040 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 5100 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 5160 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 5220 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 5280 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 5340 atttccccga aaagtgccac ctgggtcgat cgacattgat tattgactag ttattaatag 5400 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt 5460 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg 5520 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat 5580 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct 5640 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg 5700 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtcgaggtg 5760 agccccacgt tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat 5820 ttatttattt tttaattatt ttgtgcagcg atgggggcgg gggggggggg ggggcccccc 5880 ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga ggcggaaagg tgcggcggca 5940 gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg 6000 ccctataaaa agcgaagcgc gcggcgggcg ggagtcgttg cgcgctgcct tccccccgtg 6060 ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca 6120 caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt ggtttaatga 6180 cggcttgttt cttttctgtg gctgcgtgaa agccttgagg ggctccggga gggccctttg 6240 tgcgggggga gcggctcggg gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc 6300 ggctccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc 6360 cgcagtgtgc gcgaggggag cgcggccggg ggcggtgccc cgcggtgcgg ggggggctgc 6420 gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg ggtgagcagg gggtgtgggc 6480 gcgtcggtcg ggctgcaacc ccccctgcac ccccctcccc gagttgctga gcacggcccg 6540 gcttcgggtg cggggctccg tacggggcgt ggcgcggggc tcgccgtgcc gggcgggggg 6600 tggcggcagg tgggggtgcc gggcggggcg gggccgcctc gggccgggga gggctcgggg 6660 gaaggggcgc ggcggccccc ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca 6720 ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc aaatctgtgc 6780 ggagccgaaa tctgggaggc gccgccgcac cccctctagc gggcgcgggg cgaagcggtg 6840 cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc 6900 ccttctccct ctccagcctc ggggctgtcc gcggggggac ggctgccttc gggggggacg 6960 gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct ctgctaacca 7020 tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta ttgtgctgtc 7080 tcatcatttt ggcaaagaa 7099 //