LOCUS       pCAGGS2                 4805 bp    DNA     circular     12-JUN-2009
DEFINITION  LMBP 3955.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 3955
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium).
            Last modified: 12-JUN-2009
            
            
COMMENT     VNTNAME|pCAGGS2|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS2" 0 4805 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "BamHI" "SspI" "BglII" "FspI" "SgrAI" "NcoI" "PvuI" "SalI" 
COMMENT         "XbaI" "EcoRI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 4805 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 2 741 0 1078 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "SspI" "aatatt" 4 0 1 2956 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BglII" "agatct" 2 0 1 91 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "FspI" "tgcgca" 4 0 1 2374 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "SgrAI" "crccggyg" 3 0 1 4393 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 2 834 0 3454 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 2 2522 0 3077 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "SalI" "gtcgac" 2 0 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 4708 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 11 0 4804 0 0 "")) 
COMMENT       (CObList 
COMMENT        #10=(CFSignal (CObList) "lac promoter" 30 0 1 617 699 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(617..699)") 
COMMENT        #11=(CFSignal (CObList) "SV40 ori" 33 0 0 841 916 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "841..916") 
COMMENT        #12=(CFSignal (CObList) "SV40 polyA early" 43 0 0 947 1021 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "947..1021") 
COMMENT        #13=(CFSignal (CObList) "SV40 polyA late" 43 0 1 947 1021 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(947..1021)") 
COMMENT        #14=(CFSignal (CObList) "pMB1 ori" 33 0 1 1136 1537 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1136..1537)") 
COMMENT        #15=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 3078 3455 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3078..3455") 
COMMENT        #16=(CFSignal (CObList) "AG promoter" 29 0 0 3461 4802 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3461..4802") 
COMMENT        #17=(CFSignal (CObList) "3' UTR of rabbit \337-globin, incl. polyA" 43 0 
COMMENT             0 91 182 0 (CStringList) (CStringList) 1 1 1 1 "91..182") 
COMMENT        #18=(CFSignal (CObList) "3' flanking region of rabbit \337-globin" 21 0 
COMMENT             0 183 539 0 (CStringList) (CStringList) 1 1 1 1 "183..539") 
COMMENT        #19=(CFSignal (CObList) "ampicillin resistance" 4 0 1 2078 2938 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(2078..2938)") 
COMMENT        #20=(CFSignal (CObList) "intron AG promoter" 15 0 0 3830 4754 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3830..4754")) (CObList) 
COMMENT       (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #21=(CFSignal (CObList) "LMBP 3955" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #22=(CFSignal (CObList) "Last modified: 12-JUN-2009" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
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COMMENT                          "DNA Plasmid 'pCAGGS2'" 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 3955." 
COMMENT                          1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Creation Date: ? (no data)" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 06/12/09 04:26" 1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 4805 bp" 1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 24-OCT-2007" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #37=(CFolderPar 
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COMMENT                          1))) "Author") 
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COMMENT                                 "" 1))) "LMBP 3955") 
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COMMENT                                 "" 1))) "Last modified: 12-JUN-2009"))) 
COMMENT                   "Annotations") 
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COMMENT                                        "Start: 2078  End: 2938 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #49=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "antibiotic resistance gene; coding sequence" 
COMMENT                                        1) 
COMMENT                                   #50=(CLinePar 
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COMMENT                                   #51=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(2078..2938)" 1))) 
COMMENT                                 "ampicillin resistance"))) "CDS (1 total)") 
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COMMENT                                        "Start: 3830  End: 4754" 1) 
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COMMENT                                        "chicken \337-actin/rabbit \337-globin hybrid intron" 
COMMENT                                        1) 
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COMMENT                                        "   3830..4754" 1))) 
COMMENT                                 "intron AG promoter"))) "Intron (1 total)") 
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COMMENT                                 "3' flanking region of rabbit \337-globin"))) 
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COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3461..4802" 1))) "AG promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
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COMMENT                                        "Start: 617   End: 699  (Complementary)" 
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COMMENT                                   #76=(CLinePar 
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COMMENT                                   #77=(CLinePar 
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COMMENT                                        "   complement(617..699)" 1))) 
COMMENT                                 "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
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COMMENT                                   #82=(CLinePar 
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COMMENT                                        "   841..916" 1))) "SV40 ori") 
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COMMENT                                        "Start: 1136  End: 1537 (Complementary)" 
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COMMENT                                   #85=(CLinePar 
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COMMENT                                   #86=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(1136..1537)" 1))) 
COMMENT                                 "pMB1 ori"))) "Replication Origin (2 total)") 
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COMMENT                                        "Start: 91    End: 182 " 1) 
COMMENT                                   #90=(CLinePar 
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COMMENT                                        "3' UTR of the rabbit \337-globin gene, incl. polyA" 
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COMMENT                                 "3' UTR of rabbit \337-globin, incl. polyA") 
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COMMENT                                        "Start: 947   End: 1021" 1) 
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COMMENT                                         "   complement(947..1021)" 1))) 
COMMENT                                 "SV40 polyA late"))) "Terminator (3 total)"))) 
COMMENT                   "Feature Map") 
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COMMENT                                            " N2: 1078 " 1))))) "BamHI: 2 sites") 
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COMMENT                            1 5 "CCATGG" "GGTACC") 
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COMMENT                            3 3 "AATATT" "TTATAA") 
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COMMENT                                            " N1: 4708 " 1))))) "XbaI: 1 site") 
COMMENT                            1 5 "TCTAGA" "AGATCT"))) 
COMMENT                    "Restriction/Methylation Map")))) 
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FEATURES             Location/Qualifiers
     terminator      91..182
                     /vntifkey="43"
                     /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA
                     /note="3' UTR of the rabbit ß-globin gene, incl. polyA"
     misc_feature    183..539
                     /vntifkey="21"
                     /label=3'\flanking\region\of\rabbit\ß-globin
                     /note="3' flanking region of the rabbit ß-globin gene"
     promoter        complement(617..699)
                     /vntifkey="30"
                     /label=lac\promoter
                     /note="E. coli lac operon promoter"
     rep_origin      841..916
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="bidirectional origin of SV40"
     terminator      947..1021
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     terminator      complement(947..1021)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     rep_origin      complement(1136..1537)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     CDS             complement(2078..2938)
                     /vntifkey="4"
                     /label=ampicillin\resistance
                     /note="antibiotic resistance gene; coding sequence"
     promoter        3078..3455
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
                     /note="human CMV-IE enhancer"
     promoter        3461..4802
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     intron          3830..4754
                     /vntifkey="15"
                     /label=intron\AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid intron"
BASE COUNT     1004 a      1273 c      1338 g      1190 t 
ORIGIN
        1 ttcctcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg 
       61 gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg 
      121 gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt 
      181 gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat 
      241 catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg 
      301 gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct 
      361 gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt 
      421 tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga 
      481 tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc 
      541 ctcgacctgc agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg 
      601 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggt 
      661 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg 
      721 ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc agcaaccata gtcccgcccc 
      781 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct 
      841 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga 
      901 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctaact tgtttattgc 
      961 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 
     1021 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat 
     1081 ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct 
     1141 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 
     1201 gctcactcaa aggcggtaat acggttatcc acagaatcag ggataacgca ggaaagaaca 
     1261 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 
     1321 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 
     1381 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 
     1441 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 
     1501 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 
     1561 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 
     1621 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 
     1681 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 
     1741 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct 
     1801 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 
     1861 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 
     1921 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 
     1981 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 
     2041 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 
     2101 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 
     2161 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 
     2221 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 
     2281 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 
     2341 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 
     2401 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 
     2461 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 
     2521 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 
     2581 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 
     2641 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 
     2701 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 
     2761 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 
     2821 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 
     2881 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 
     2941 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 
     3001 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 
     3061 tgccacctgg gtcgatcgac attgattatt gactagttat taatagtaat caattacggg 
     3121 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 
     3181 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 
     3241 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 
     3301 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga 
     3361 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg 
     3421 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg 
     3481 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta 
     3541 attattttgt gcagcgatgg gggcgggggg gggggggggg ccccccccag gcggggcggg 
     3601 gcggggcgag gggcggggcg gggcgaggcg gaaaggtgcg gcggcagcca atcagagcgg 
     3661 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg 
     3721 aagcgcgcgg cgggcgggag tcgttgcgcg ctgccttccc cccgtgcccc gctccgccgc 
     3781 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg 
     3841 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt 
     3901 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg 
     3961 ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc 
     4021 cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga 
     4081 ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg 
     4141 ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct 
     4201 gcaacccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg 
     4261 gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg 
     4321 ggtgccgggc ggggcggggc cgcctcgggc cggggagggc tcgggggaag gggcgcggcg 
     4381 gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt 
     4441 aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg 
     4501 ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga 
     4561 aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc 
     4621 agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt 
     4681 tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct 
     4741 tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca 
     4801 aagaa 
//
