ID pCAGGS2 standard; circular DNA; ; 4805 BP. DE LMBP 3955. CC Accession number: LMBP 3955 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium). CC Last modified: 12-JUN-2009 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT terminator 91..182 FT /vntifkey="43" FT /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA FT /note="3' UTR of the rabbit ß-globin gene, incl. polyA" FT misc_feature 183..539 FT /vntifkey="21" FT /label=3'\flanking\region\of\rabbit\ß-globin FT /note="3' flanking region of the rabbit ß-globin gene" FT promoter complement(617..699) FT /vntifkey="30" FT /label=lac\promoter FT /note="E. coli lac operon promoter" FT rep_origin 841..916 FT /vntifkey="33" FT /label=SV40\ori FT /note="bidirectional origin of SV40" FT terminator 947..1021 FT /vntifkey="43" FT /label=SV40\polyA\early FT /note="polyA signal of the SV40 early region" FT terminator complement(947..1021) FT /vntifkey="43" FT /label=SV40\polyA\late FT /note="polyA signal of the SV40 late region" FT rep_origin complement(1136..1537) FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT CDS complement(2078..2938) FT /vntifkey="4" FT /label=ampicillin\resistance FT /note="antibiotic resistance gene; coding sequence" FT promoter 3078..3455 FT /vntifkey="29" FT /label=hCMV-IE\enhancer FT /note="human CMV-IE enhancer" FT promoter 3461..4802 FT /vntifkey="29" FT /label=AG\promoter FT /note="chicken ß-actin/rabbit ß-globin hybrid promoter" FT intron 3830..4754 FT /vntifkey="15" FT /label=intron\AG\promoter FT /note="chicken ß-actin/rabbit ß-globin hybrid intron" SQ Sequence 4805 BP; 1004 A; 1273 C; 1338 G; 1190 t; ttcctcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg 60 gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg 120 gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt 180 gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat 240 catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg 300 gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct 360 gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt 420 tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga 480 tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc 540 ctcgacctgc agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg 600 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggt 660 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg 720 ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc agcaaccata gtcccgcccc 780 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct 840 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga 900 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctaact tgtttattgc 960 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 1020 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat 1080 ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct 1140 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 1200 gctcactcaa aggcggtaat acggttatcc acagaatcag ggataacgca ggaaagaaca 1260 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 1320 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 1380 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 1440 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 1500 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 1560 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 1620 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 1680 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 1740 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct 1800 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 1860 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 1920 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 1980 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 2040 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 2100 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 2160 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 2220 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 2280 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 2340 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 2400 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 2460 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 2520 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 2580 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 2640 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 2700 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 2760 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 2820 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 2880 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 2940 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 3000 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 3060 tgccacctgg gtcgatcgac attgattatt gactagttat taatagtaat caattacggg 3120 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 3180 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 3240 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 3300 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga 3360 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg 3420 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg 3480 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta 3540 attattttgt gcagcgatgg gggcgggggg gggggggggg ccccccccag gcggggcggg 3600 gcggggcgag gggcggggcg gggcgaggcg gaaaggtgcg gcggcagcca atcagagcgg 3660 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg 3720 aagcgcgcgg cgggcgggag tcgttgcgcg ctgccttccc cccgtgcccc gctccgccgc 3780 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg 3840 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt 3900 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg 3960 ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc 4020 cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga 4080 ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg 4140 ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct 4200 gcaacccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg 4260 gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg 4320 ggtgccgggc ggggcggggc cgcctcgggc cggggagggc tcgggggaag gggcgcggcg 4380 gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt 4440 aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg 4500 ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga 4560 aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc 4620 agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt 4680 tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct 4740 tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca 4800 aagaa 4805 //