ID pCAGGS-FLAGmA20f5 standard; circular DNA; ; 6544 BP. DE LMBP 3956. CC Accession number: LMBP 3956 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 9-JUN-2009 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT misc_marker 13..36 FT /vntifkey="22" FT /label=FLAG\epitope FT promoter complement(2356..2438) FT /vntifkey="30" FT /label=lac\promoter FT terminator 1830..1921 FT /vntifkey="43" FT /label=3'\UTR\of\ß-globin,\incl.\polyA FT promoter 4817..5194 FT /vntifkey="29" FT /label=hCMV-IE\enhancer FT CDS complement(3817..4677) FT /vntifkey="4" FT /label=ampicillin\resistance FT rep_origin 2580..2655 FT /vntifkey="33" FT /label=SV40\ori FT rep_origin complement(2875..3276) FT /vntifkey="33" FT /label=pMB1\ori FT intron 5569..6493 FT /vntifkey="15" FT /label=intron\AG\promoter FT CDS 46..1815 FT /vntifkey="4" FT /label=mA20\(1-590) FT misc_feature 1922..2278 FT /vntifkey="21" FT /label=3'\flanking\region\of\rabbit\ß-globin FT terminator 2686..2760 FT /vntifkey="43" FT /label=SV40\polyA\early FT terminator complement(2686..2760) FT /vntifkey="43" FT /label=SV40\polyA\late FT promoter 5200..6541 FT /vntifkey="29" FT /label=AG\promoter SQ Sequence 6544 BP; 1490 A; 1738 C; 1742 G; 1574 t; ttcctcgaga tggactacaa ggacgacgat gacaagggta ccgccatggc tgaacaactt 60 cttcctcagg ctttgtattt gagcaatatg cggaaagctg tgaagatacg agagagaacc 120 ccagaagaca ttttcaaacc taccaatggg atcatctatc actttaaaac catgcaccga 180 tacacgctgg agatgttcag aacatgccag ttttgcccac agttccgaga gatcatccac 240 aaagcactta ttgacagaag tgtccaggct tccctggaaa gccagaagaa gctcaactgg 300 tgtcgtgaag tcaggaagct cgtggctctg aaaaccaatg gtgatggaaa ctgcctcatg 360 catgcagctt gtcagtacat gtggggtgtt caggatactg acctggtcct gaggaaggcc 420 ctctgcagca cccttaagga gacagacact cggaacttta aattccgctg gcagctggaa 480 tctctgaaat ctcaggaatt tgtggaaaca ggactttgct acgacactcg gaactggaat 540 gacgaatggg acaacttggt caaaatggca tcagcagaca cacctgcagc ccgaagtgga 600 cttcagtaca attccctgga agaaatccac atatttgtcc tcagcaacat cctcagaaga 660 cccatcattg tcatttcaga caaaatgcta agaagtttgg aatctggttc caattttgct 720 cctttgaaag tgggtgggat ttatctgcct cttcactggc ctgcccagga gtgttacaga 780 tatcccatcg tcctaggcta tgacagccag cactttgtac ccctggtgac cctgaaggac 840 agtggacctg aacttcgcgc tgttccactt gttaacagag accggggtag gtttgaagac 900 ttaaaagttc acttcttgac agatcctgag aatgagatga aggaaaagct tctaaaggag 960 tacttgatag tgatggagat ccctgtgcaa ggctgggacc acggcacgac tcacctgatc 1020 aacgctgcaa aattggatga agctaactta cccaaagaaa taaatttggt agacgattac 1080 tttgagcttg ttcagcacga atacaagaaa tggcaggaga acagcgatca ggccaggaga 1140 gcggcacatg cgcagaaccc cttggagcct tccacacccc agctatcact catggatata 1200 aaatgtgaga cacccaactg tcctttcttc atgtccgtga acactcagcc tttatgccac 1260 gaatgctcag agaggcgcca aaagaatcag agcaagctcc caaagctgaa ctcgaagcta 1320 ggccctgaag gactcccagg cgtgggactt ggctcctcaa actggagccc cgaggaaacc 1380 gctggaggac ctcattcagc cccacccaca gcacccagcc tttttctctt cagtgagacc 1440 actgcaatga agtgcaggag tcctgggtgc ccttttactt tgaatgtgca gcataatgga 1500 ttctgtgagc gttgccacgc ccggcagatt aatgccagcc acaccgcaga ccctggaaag 1560 tgccaagcct gccttcagga tgtcactcgg acctttaatg gcatctgcag tacctgtttc 1620 aaaaggacta cagcagagcc cagctccagc ctcacttcca gtatccctgc ctcctgtcac 1680 caacgctcca agtctgaccc ctcacaactc atccaaagtc tcactccaca ctcttgccac 1740 cggactggaa atgtctctcc ttctggctgc ctctcccagg ctgcacggac tccaggagac 1800 agagcaggga caagctaagt gtcgacgaag atctttttcc ctctgccaaa aattatgggg 1860 acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt attttcattg 1920 caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc 1980 atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg 2040 ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg 2100 tccattcctt attccataga aaagccttga cttgaggtta gatttttttt atattttgtt 2160 ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat 2220 ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatccc 2280 tcgacctgca gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt 2340 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctgggtg 2400 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg 2460 gaaacctgtc gtgccagcgg atccgcatct caattagtca gcaaccatag tcccgcccct 2520 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg 2580 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa 2640 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctaactt gtttattgca 2700 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 2760 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc 2820 cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 2880 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 2940 ctcactcaaa ggcggtaata cggttatcca cagaatcagg gataacgcag gaaagaacat 3000 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 3060 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 3120 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 3180 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 3240 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 3300 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 3360 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 3420 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 3480 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt 3540 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 3600 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 3660 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 3720 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 3780 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc 3840 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta 3900 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga 3960 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg 4020 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc 4080 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat 4140 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag 4200 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat 4260 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa 4320 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa 4380 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga 4440 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg 4500 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc 4560 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg 4620 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact 4680 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat 4740 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 4800 gccacctggg tcgatcgaca ttgattattg actagttatt aatagtaatc aattacgggg 4860 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg 4920 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata 4980 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc 5040 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac 5100 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg 5160 cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc 5220 ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa 5280 ttattttgtg cagcgatggg ggcggggggg gggggggggc cccccccagg cggggcgggg 5340 cggggcgagg ggcggggcgg ggcgaggcgg aaaggtgcgg cggcagccaa tcagagcggc 5400 gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga 5460 agcgcgcggc gggcgggagt cgttgcgcgc tgccttcccc ccgtgccccg ctccgccgcc 5520 gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg 5580 gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt 5640 ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc 5700 tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc 5760 ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag 5820 gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc 5880 tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg 5940 caaccccccc tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg 6000 ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg 6060 gtgccgggcg gggcggggcc gcctcgggcc ggggagggct cgggggaagg ggcgcggcgg 6120 cccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta 6180 atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg 6240 gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa 6300 ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca 6360 gcctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt 6420 cggcttctgg cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt 6480 ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa 6540 agaa 6544 //