ID pCAGGS-FLAGmA20f6 standard; circular DNA; ; 6871 BP. DE LMBP 3957. CC Accession number: LMBP 3957 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 11-JUN-2009 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT misc_marker 13..36 FT /vntifkey="22" FT /label=FLAG\epitope FT promoter complement(2683..2765) FT /vntifkey="30" FT /label=lac\promoter FT terminator 2157..2248 FT /vntifkey="43" FT /label=3'\UTR\of\ß-globin,\incl.\polyA FT promoter 5144..5521 FT /vntifkey="29" FT /label=hCMV-IE\enhancer FT CDS complement(4144..5004) FT /vntifkey="4" FT /label=ampicillin\resistance FT rep_origin 2907..2982 FT /vntifkey="33" FT /label=SV40\ori FT rep_origin complement(3202..3603) FT /vntifkey="33" FT /label=pMB1\ori FT intron 5896..6820 FT /vntifkey="15" FT /label=intron\AG\promoter FT CDS 46..2142 FT /vntifkey="4" FT /label=mA20\(1-699) FT misc_feature 2249..2605 FT /vntifkey="21" FT /label=3'\flanking\region\of\rabbit\ß-globin FT terminator 3013..3087 FT /vntifkey="43" FT /label=SV40\polyA\early FT terminator complement(3013..3087) FT /vntifkey="43" FT /label=SV40\polyA\late FT promoter 5527..6868 FT /vntifkey="29" FT /label=AG\promoter SQ Sequence 6871 BP; 1586 A; 1816 C; 1829 G; 1640 t; ttcctcgaga tggactacaa ggacgacgat gacaagggta ccgccatggc tgaacaactt 60 cttcctcagg ctttgtattt gagcaatatg cggaaagctg tgaagatacg agagagaacc 120 ccagaagaca ttttcaaacc taccaatggg atcatctatc actttaaaac catgcaccga 180 tacacgctgg agatgttcag aacatgccag ttttgcccac agttccgaga gatcatccac 240 aaagcactta ttgacagaag tgtccaggct tccctggaaa gccagaagaa gctcaactgg 300 tgtcgtgaag tcaggaagct cgtggctctg aaaaccaatg gtgatggaaa ctgcctcatg 360 catgcagctt gtcagtacat gtggggtgtt caggatactg acctggtcct gaggaaggcc 420 ctctgcagca cccttaagga gacagacact cggaacttta aattccgctg gcagctggaa 480 tctctgaaat ctcaggaatt tgtggaaaca ggactttgct acgacactcg gaactggaat 540 gacgaatggg acaacttggt caaaatggca tcagcagaca cacctgcagc ccgaagtgga 600 cttcagtaca attccctgga agaaatccac atatttgtcc tcagcaacat cctcagaaga 660 cccatcattg tcatttcaga caaaatgcta agaagtttgg aatctggttc caattttgct 720 cctttgaaag tgggtgggat ttatctgcct cttcactggc ctgcccagga gtgttacaga 780 tatcccatcg tcctaggcta tgacagccag cactttgtac ccctggtgac cctgaaggac 840 agtggacctg aacttcgcgc tgttccactt gttaacagag accggggtag gtttgaagac 900 ttaaaagttc acttcttgac agatcctgag aatgagatga aggaaaagct tctaaaggag 960 tacttgatag tgatggagat ccctgtgcaa ggctgggacc acggcacgac tcacctgatc 1020 aacgctgcaa aattggatga agctaactta cccaaagaaa taaatttggt agacgattac 1080 tttgagcttg ttcagcacga atacaagaaa tggcaggaga acagcgatca ggccaggaga 1140 gcggcacatg cgcagaaccc cttggagcct tccacacccc agctatcact catggatata 1200 aaatgtgaga cacccaactg tcctttcttc atgtccgtga acactcagcc tttatgccac 1260 gaatgctcag agaggcgcca aaagaatcag agcaagctcc caaagctgaa ctcgaagcta 1320 ggccctgaag gactcccagg cgtgggactt ggctcctcaa actggagccc cgaggaaacc 1380 gctggaggac ctcattcagc cccacccaca gcacccagcc tttttctctt cagtgagacc 1440 actgcaatga agtgcaggag tcctgggtgc ccttttactt tgaatgtgca gcataatgga 1500 ttctgtgagc gttgccacgc ccggcagatt aatgccagcc acaccgcaga ccctggaaag 1560 tgccaagcct gccttcagga tgtcactcgg acctttaatg gcatctgcag tacctgtttc 1620 aaaaggacta cagcagagcc cagctccagc ctcacttcca gtatccctgc ctcctgtcac 1680 caacgctcca agtctgaccc ctcacaactc atccaaagtc tcactccaca ctcttgccac 1740 cggactggaa atgtctctcc ttctggctgc ctctcccagg ctgcacggac tccaggagac 1800 agagcaggga caagcaagtg caggaaagct ggctgcatgt attttgggac tccagaaaac 1860 aagggctttt gcactctatg tttcatcgaa tacagagaaa ataagcagtc tgttactgcc 1920 tctgcgaaag ctggttcccc ggcccccagg ttccagaaca atgtcccgtg cctgggcagg 1980 gagtgcggca cactcggaag caccatgttt gaagggtact gtcagaagtg tttcatcgaa 2040 gctcagaacc agagattcca tgaagcaaga agaacggaag aacagctgag atcaagccag 2100 catagagaca tgcctcgaac tacacaggta gcctcaaggc tgtaagtgtc gacgaagatc 2160 tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg 2220 gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac 2280 tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga 2340 gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta taaagaggtc 2400 atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt 2460 gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat 2520 tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag 2580 ctgtccctct tctcttatgg agatccctcg acctgcagcc caagcttggc gtaatcatgg 2640 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc 2700 ggaagcataa agtgtaaagc ctgggtgcct aatgagtgag ctaactcaca ttaattgcgt 2760 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc cgcatctcaa 2820 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag 2880 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc 2940 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt 3000 ttgcaaaaag ctaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 3060 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 3120 atcaatgtat cttatcatgt ctggatccgc tgcattaatg aatcggccaa cgcgcgggga 3180 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 3240 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 3300 aatcagggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 3360 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 3420 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 3480 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 3540 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 3600 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 3660 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 3720 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 3780 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat 3840 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 3900 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 3960 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 4020 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 4080 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 4140 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 4200 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 4260 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 4320 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 4380 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 4440 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 4500 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 4560 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 4620 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 4680 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 4740 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 4800 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 4860 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 4920 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 4980 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 5040 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 5100 ggttccgcgc acatttcccc gaaaagtgcc acctgggtcg atcgacattg attattgact 5160 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc 5220 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg 5280 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa 5340 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca 5400 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac 5460 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc 5520 atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc 5580 ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg 5640 ggggggcccc ccccaggcgg ggcggggcgg ggcgaggggc ggggcggggc gaggcggaaa 5700 ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg 5760 cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgt tgcgcgctgc 5820 cttccccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc 5880 gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagcgc 5940 ttggtttaat gacggcttgt ttcttttctg tggctgcgtg aaagccttga ggggctccgg 6000 gagggccctt tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg 6060 agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg 6120 ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc 6180 ggggggggct gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca 6240 gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc ccgagttgct 6300 gagcacggcc cggcttcggg tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg 6360 ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg 6420 gagggctcgg gggaaggggc gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc 6480 gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc 6540 ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta gcgggcgcgg 6600 ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc 6660 gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg acggctgcct 6720 tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc 6780 ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt 6840 tattgtgctg tctcatcatt ttggcaaaga a 6871 //