ID pCAGGSFLAGmCASP-11m2 standard; circular DNA; ; 5888 BP. DE LMBP 3972. CC Accession number: LMBP 3972 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium). CC Last modified: 15-FEB-2008 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT mutation 799..804 FT /vntifkey="62" FT /label=C254S FT /note="WT: GCC.TGC" FT CDS 43..1164 FT /vntifkey="4" FT /label=mCASP-11\mutant FT /note="mouse cysteinyl aspartate specific proteinase 11 cDNA; mutated sequence" FT misc_marker 13..36 FT /vntifkey="22" FT /label=FLAG\epitope FT /note="N-terminal FLAG epitope tag" FT terminator 1174..1265 FT /vntifkey="43" FT /label=ß-globin\polyA FT /note="3' UTR of the rabbit ß-globin gene, incl. polyA" FT misc_feature 1266..1622 FT /vntifkey="21" FT /label=3'\FR\of\ß-globin FT /note="3' flanking region of the rabbit ß-globin gene" FT promoter complement(1700..1782) FT /vntifkey="30" FT /label=lac\promoter FT /note="Escherichia coli lac operon promoter" FT rep_origin 1924..1999 FT /vntifkey="33" FT /label=SV40\ori FT /note="bidirectional origin of simian virus 40" FT terminator 2030..2104 FT /vntifkey="43" FT /label=SV40\polyA\early FT /note="polyA signal of the SV40 early region" FT terminator complement(2030..2104) FT /vntifkey="43" FT /label=SV40\polyA\late FT /note="polyA signal of the SV40 late region" FT rep_origin complement(2219..2620) FT /vntifkey="33" FT /label=pMB1\ori FT /note="Escherichia coli plasmid pMB1 origin" FT CDS complement(3161..4021) FT /vntifkey="4" FT /label=ampicillin FT /note="antibiotic resistance gene; coding sequence" FT promoter 4161..4538 FT /vntifkey="29" FT /label=hCMV-IE\enhancer FT /note="human cytomegalovirus immediate early enhancer" FT promoter 4544..5885 FT /vntifkey="29" FT /label=AG\promoter FT /note="chicken ß-actin/rabbit ß-globin hybrid promoter" FT intron 4913..5837 FT /vntifkey="15" FT /label=intron\AG\promoter FT /note="chicken ß-actin/rabbit ß-globin hybrid intron" SQ Sequence 5888 BP; 1362 A; 1505 C; 1579 G; 1442 t; ttcctcgaga tggactacaa ggacgacgat gacaagggta ccatggctga aaacaaacac 60 cctgacaaac cacttaaggt gttggaacag ctgggcaaag aagtccttac ggagtaccta 120 gaaaaattag tacaaagcaa tgtactgaaa ttaaaggagg aagataaaca aaaatttaac 180 aatgctgaac gcagtgacaa gcgttgggtt tttgtagatg ccatgaaaaa gaaacacagc 240 aaagtaggtg aaatgcttct ccagacattc ttcagtgtgg acccaggcag ccaccatggt 300 gaagctaatc tggaaatgga ggaaccagaa gaatcattga acactctcaa gctttgttcc 360 cctgaagagt tcacaaggct ttgcagagaa aagacacaag aaatttaccc aataaaggag 420 gccaatggcc gtacacgaaa ggctcttatc atatgcaata cagagttcaa acatctctca 480 ctgaggtatg gggctaactt tgacatcatt ggtatgaaag gccttcttga agacttaggc 540 tacgatgtgg tggtgaaaga ggagcttaca gcagagggca tggagtcaga gatgaaagac 600 tttgctgcac tctcagaaca ccagacatca gacagcacat tcctggtgct aatgtctcat 660 ggcacactgc atggcatttg tggaacaatg cacagtgaaa aaactccaga tgtgctacag 720 tatgatacca tctatcagat attcaacaat tgccactgtc caggtctacg agacaaaccc 780 aaagtcatca ttgtgcaggc tagcagaggt gggaactctg gagaaatgtg gatcagagag 840 tcttcaaaac cccagttgtg cagaggtgta gatctaccta ggaatatgga agctgatgct 900 gtcaagctga gccacgtgga gaaggacttc attgccttct actctacaac cccacatcac 960 ttgtcctacc gagacaaaac aggaggctct tacttcatca ctagactcat ttcctgcttc 1020 cggaaacatg cttgctcttg tcatctcttt gatatattcc tgaaggtgca acaatcattt 1080 gaaaaggcaa gtattcattc ccagatgccc accattgatc gggcaacctt gacgagatat 1140 ttctacgtct ttcctggcaa ctgagtcgac gaagatcttt ttccctctgc caaaaattat 1200 ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc 1260 attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca 1320 aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg 1380 ctggctgcca tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct 1440 gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt 1500 tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc 1560 agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct cttatggaga 1620 tccctcgacc tgcagcccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa 1680 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 1740 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag 1800 tcgggaaacc tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc atagtcccgc 1860 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg 1920 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc 1980 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagcta acttgtttat 2040 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 2100 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 2160 gatccgctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 2220 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 2280 tcagctcact caaaggcggt aatacggtta tccacagaat cagggataac gcaggaaaga 2340 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 2400 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 2460 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 2520 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 2580 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 2640 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 2700 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 2760 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 2820 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta 2880 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 2940 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 3000 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 3060 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 3120 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 3180 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 3240 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 3300 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 3360 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 3420 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 3480 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 3540 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 3600 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 3660 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 3720 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 3780 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 3840 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 3900 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 3960 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 4020 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 4080 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 4140 aagtgccacc tgggtcgatc gacattgatt attgactagt tattaatagt aatcaattac 4200 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg 4260 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc 4320 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac 4380 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa 4440 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac 4500 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt 4560 ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt 4620 ttaattattt tgtgcagcga tgggggcggg gggggggggg gggccccccc caggcggggc 4680 ggggcggggc gaggggcggg gcggggcgag gcggaaaggt gcggcggcag ccaatcagag 4740 cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa 4800 gcgaagcgcg cggcgggcgg gagtcgttgc gcgctgcctt ccccccgtgc cccgctccgc 4860 cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg 4920 gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc 4980 ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag 5040 cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct 5100 gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg 5160 cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa 5220 aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg 5280 gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc 5340 ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt 5400 gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aaggggcgcg 5460 gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat 5520 ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat 5580 ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca 5640 ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc 5700 tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg 5760 ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct 5820 tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg 5880 gcaaagaa 5888 //