LOCUS       pCAGGSFLAGmCASP-11 6223 bp dsDNACircular
DEFINITION  LMBP 3973.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     promoter        complement(2035..2117)
                     /label=lac\promoter
     CDS             43..1164
                     /label=mCASP-11
     misc_difference 10..36
                     /label=FLAG-tag
     terminator      1509..1600
                     /label=ß-globin\polyA
     rep_origin      2259..2334
                     /label=SV40\ori
     terminator      2364..2439
                     /label=SV40\polyA\early
     terminator      complement(2364..2439)
                     /label=SV40\polyA\late
     rep_origin      complement(2554..2955)
                     /label=pMB1\ori
     CDS             complement(3496..4356)
                     /label=AMP
     promoter        4496..4873
                     /label=hCMV\enhancer
     promoter        5248..6000
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
                     /label=AG\promoter
     intron          5248..6172
                     /label=intron\AG\promoter
ORIGIN
        1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccatggctga aaacaaacac 
       61 cctgacaaac cacttaaggt gttggaacag ctgggcaaag aagtccttac ggagtaccta 
      121 gaaaaattag tacaaagcaa tgtactgaaa ttaaaggagg aagataaaca aaaatttaac 
      181 aatgctgaac gcagtgacaa gcgttgggtt tttgtagatg ccatgaaaaa gaaacacagc 
      241 aaagtaggtg aaatgcttct ccagacattc ttcagtgtgg acccaggcag ccaccatggt 
      301 gaagctaatc tggaaatgga ggaaccagaa gaatcattga acactctcaa gctttgttcc 
      361 cctgaagagt tcacaaggct ttgcagagaa aagacacaag aaatttaccc aataaaggag 
      421 gccaatggcc gtacacgaaa ggctcttatc atatgcaata cagagttcaa acatctctca 
      481 ctgaggtatg gggctaactt tgacatcatt ggtatgaaag gccttcttga agacttaggc 
      541 tacgatgtgg tggtgaaaga ggagcttaca gcagagggca tggagtcaga gatgaaagac 
      601 tttgctgcac tctcagaaca ccagacatca gacagcacat tcctggtgct aatgtctcat 
      661 ggcacactgc atggcatttg tggaacaatg cacagtgaaa aaactccaga tgtgctacag 
      721 tatgatacca tctatcagat attcaacaat tgccactgtc caggtctacg agacaaaccc 
      781 aaagtcatca ttgtgcaggc ctgcagaggt gggaactctg gagaaatgtg gatcagagag 
      841 tcttcaaaac cccagttgtg cagaggtgta gatctaccta ggaatatgga agctgatgct 
      901 gtcaagctga gccacgtgga gaaggacttc attgccttct actctacaac cccacatcac 
      961 ttgtcctacc gagacaaaac aggaggctct tacttcatca ctagactcat ttcctgcttc 
     1021 cggaaacatg cttgctcttg tcatctcttt gatatattcc tgaaggtgca acaatcattt 
     1081 gaaaaggcaa gtattcattc ccagatgccc accattgatc gggcaacctt gacgagatat 
     1141 ttctacctct ttcctggcaa ctgagaacaa agcaacaagc aactgaatct catttcttca 
     1201 gcttgaagaa gtgatcttgg ccaaggatca cattctattc ctgaaattcc agaactagtg 
     1261 aaattaagga aagaatactt atgaattcaa gaccagccta agcaacacag tgggattctg 
     1321 ttccatagac aagcaaacaa gcccaaaaaa aaaaaaaaac tcgtgccgaa ttcgatctct 
     1381 ttttttgggt ttggtggggt atcttcatca tcgaatagat agttatatac atcatccatt 
     1441 gtagtggtat taaacatccc tgtagtgatt ccaaacgcgc caatgccctg gctcacaaat 
     1501 accactgaga tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc 
     1561 atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt 
     1621 tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt 
     1681 atttggttta gagtttggca acatatgccc atatgctggc tgccatgaac aaaggttggc 
     1741 tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta ttccatagaa 
     1801 aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt ttttctttaa 
     1861 catccctaaa attttcctta catgttttac tagccagatt tttcctcctc tcctgactac 
     1921 tcccagtcat agctgtccct cttctcttat ggagatccct cgacctgcag cccaagcttg 
     1981 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 
     2041 aacatacgag ccggaagcat aaagtgtaaa gcctgggtgc ctaatgagtg agctaactca 
     2101 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga 
     2161 tccgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct 
     2221 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc 
     2281 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg 
     2341 aggcctaggc ttttgcaaaa agctaacttg tttattgcag cttataatgg ttacaaataa 
     2401 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 
     2461 ttgtccaaac tcatcaatgt atcttatcat gtctggatcc gctgcattaa tgaatcggcc 
     2521 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact 
     2581 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 
     2641 ggttatccac agaatcaggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 
     2701 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 
     2761 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 
     2821 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 
     2881 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 
     2941 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 
     3001 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 
     3061 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 
     3121 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac 
     3181 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 
     3241 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 
     3301 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 
     3361 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 
     3421 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 
     3481 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 
     3541 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 
     3601 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 
     3661 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 
     3721 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 
     3781 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 
     3841 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 
     3901 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 
     3961 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 
     4021 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 
     4081 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 
     4141 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 
     4201 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 
     4261 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 
     4321 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 
     4381 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 
     4441 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgggt cgatcgacat 
     4501 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat 
     4561 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac 
     4621 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc 
     4681 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg 
     4741 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat 
     4801 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc 
     4861 atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc 
     4921 ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg 
     4981 gcgggggggg ggggggggcc ccccccaggc ggggcggggc ggggcgaggg gcggggcggg 
     5041 gcgaggcgga aaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt 
     5101 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc 
     5161 gttgcgcgct gccttccccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg 
     5221 ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc 
     5281 tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg tgaaagcctt 
     5341 gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt 
     5401 gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg 
     5461 cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt 
     5521 gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg 
     5581 ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct gcacccccct 
     5641 ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg 
     5701 gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg 
     5761 cctcgggccg gggagggctc gggggaaggg gcgcggcggc ccccggagcg ccggcggctg 
     5821 tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg 
     5881 acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc 
     5941 tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct 
     6001 tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct gtccgcgggg 
     6061 ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg 
     6121 cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg 
     6181 caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaa 
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