LOCUS       p423MET25   6404 bp dsDNA    Circular
DEFINITION  LMBP 4013.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     promoter        complement(2398..2789)
                     /label=MET25\promoter
     CDS             504..1163
                     /label=HIS3
     CDS             complement(4003..4863)
                     /label=AMP
     rep_origin      complement(4995..6052)
                     /note="2mu-ori incl. STB"
                     /label=2mu\ori
     promoter        2049..2065
                     /label=T7\promoter
     terminator      complement(2086..2333)
                     /label=CYC1\terminator
     promoter        complement(2805..2821)
                     /label=T3\promoter
     promoter        complement(2885..2968)
                     /label=lac\promoter
     rep_origin      3060..3462
                     /label=pMB1\ori
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accataaatt cccgttttaa gagcttggtg agcgctagga gtcactgcca ggtatcgttt 
      241 gaacacggca ttagtcaggg aagtcataac acagtccttt cccgcaattt tctttttcta 
      301 ttactcttgg cctcctctag tacactctat atttttttat gcctcggtaa tgattttcat 
      361 tttttttttt cccctagcgg atgactcttt ttttttctta gcgattggca ttatcacata 
      421 atgaattata cattatataa agtaatgtga tttcttcgaa gaatatacta aaaaatgagc 
      481 aggcaagata aacgaaggca aagatgacag agcagaaagc cctagtaaag cgtattacaa 
      541 atgaaaccaa gattcagatt gcgatctctt taaagggtgg tcccctagcg atagagcact 
      601 cgatcttccc agaaaaagag gcagaagcag tagcagaaca ggccacacaa tcgcaagtga 
      661 ttaacgtcca cacaggtata gggtttctgg accatatgat acatgctctg gccaagcatt 
      721 ccggctggtc gctaatcgtt gagtgcattg gtgacttaca catagacgac catcacacca 
      781 ctgaagactg cgggattgct ctcggtcaag cttttaaaga ggccctactg gcgcgtggag 
      841 taaaaaggtt tggatcagga tttgcgcctt tggatgaggc actttccaga gcggtggtag 
      901 atctttcgaa caggccgtac gcagttgtcg aacttggttt gcaaagggag aaagtaggag 
      961 atctctcttg cgagatgatc ccgcattttc ttgaaagctt tgcagaggct agcagaatta 
     1021 ccctccacgt tgattgtctg cgaggcaaga atgatcatca ccgtagtgag agtgcgttca 
     1081 aggctcttgc ggttgccata agagaagcca cctcgcccaa tggtaccaac gatgttccct 
     1141 ccaccaaagg tgttcttatg tagtgacacc gattatttaa agctgcagca tacgatatat 
     1201 atacatgtgt atatatgtat acctatgaat gtcagtaagt atgtatacga acagtatgat 
     1261 actgaagatg acaaggtaat gcatcattct atacgtgtca ttctgaacga ggcgcgcttt 
     1321 ccttttttct ttttgctttt tctttttttt tctcttgaac tcgacggatc tatgcggtgt 
     1381 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta aacgttaata 
     1441 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg 
     1501 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc 
     1561 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa 
     1621 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt 
     1681 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac 
     1741 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta 
     1801 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg 
     1861 cgccgctaca gggcgcgtcg cgccattcgc cattcaggct gcgcaactgt tgggaagggc 
     1921 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc 
     1981 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg 
     2041 agcgcgcgta atacgactca ctatagggcg aattgggtac cggccgcaaa ttaaagcctt 
     2101 cgagcgtccc aaaaccttct caagcaaggt tttcagtata atgttacatg cgtacacgcg 
     2161 tctgtacaga aaaaaaagaa aaatttgaaa tataaataac gttcttaata ctaacataac 
     2221 tataaaaaaa taaataggga cctagacttc aggttgtcta actccttcct tttcggttag 
     2281 agcggatgtg gggggagggc gtgaatgtaa gcgtgacata actaattaca tgactcgagg 
     2341 tcgacggtat cgataagctt gatatcgaat tcctgcagcc cgggggatcc actagttcta 
     2401 gatgtatgga tgggggtaat agaattgtat ctatgtatct gacgaccctg tattacacga 
     2461 aggaaatagt agatgaaaag acaagagagc aagaaaaagg aaaaacttcc tttctaacag 
     2521 acgctttact taaccttata tatatcttat ttttttcatc gagcgtgttc aattggacaa 
     2581 ggtgccacct tttcgacact tcggttatta tgttacacag ttttcatgag gatggcgcct 
     2641 tgactaactt aattagtcat ttgccaacca ccacagttcc ccaatatcga cagcttcacg 
     2701 tgccatttgc cattttgcga tcatgtgacc tcaagagaaa aagcaaaaaa taatgagagc 
     2761 taagggattc gaacccttgc atccgagctc cagcttttgt tccctttagt gagggttaat 
     2821 tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 
     2881 aattccacac aacataggag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 
     2941 gaggtaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 
     3001 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 
     3061 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 
     3121 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 
     3181 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 
     3241 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 
     3301 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 
     3361 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 
     3421 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 
     3481 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 
     3541 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 
     3601 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 
     3661 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 
     3721 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 
     3781 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 
     3841 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 
     3901 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 
     3961 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 
     4021 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 
     4081 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 
     4141 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 
     4201 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 
     4261 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 
     4321 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 
     4381 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 
     4441 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 
     4501 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 
     4561 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 
     4621 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 
     4681 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 
     4741 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 
     4801 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 
     4861 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 
     4921 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 
     4981 aaaagtgcca cctgaacgaa gcatctgtgc ttcattttgt agaacaaaaa tgcaacgcga 
     5041 gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag aaatgcaacg 
     5101 cgaaagcgct attttaccaa cgaagaatct gtgcttcatt tttgtaaaac aaaaatgcaa 
     5161 cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg 
     5221 caacgcgaga gcgctatttt accaacaaag aatctatact tcttttttgt tctacaaaaa 
     5281 tgcatcccga gagcgctatt tttctaacaa agcatcttag attacttttt ttctcctttg 
     5341 tgcgctctat aatgcagtct cttgataact ttttgcactg taggtccgtt aaggttagaa 
     5401 gaaggctact ttggtgtcta ttttctcttc cataaaaaaa gcctgactcc acttcccgcg 
     5461 tttactgatt actagcgaag ctgcgggtgc attttttcaa gataaaggca tccccgatta 
     5521 tattctatac cgatgtggat tgcgcatact ttgtgaacag aaagtgatag cgttgatgat 
     5581 tcttcattgg tcagaaaatt atgaacggtt tcttctattt tgtctctata tactacgtat 
     5641 aggaaatgtt tacattttcg tattgttttc gattcactct atgaatagtt cttactacaa 
     5701 tttttttgtc taaagagtaa tactagagat aaacataaaa aatgtagagg tcgagtttag 
     5761 atgcaagttc aaggagcgaa aggtggatgg gtaggttata tagggatata gcacagagat 
     5821 atatagcaaa gagatacttt tgagcaatgt ttgtggaagc ggtattcgca atattttagt 
     5881 agctcgttac agtccggtgc gtttttggtt ttttgaaagt gcgtcttcag agcgcttttg 
     5941 gttttcaaaa gcgctctgaa gttcctatac tttctagaga ataggaactt cggaatagga 
     6001 acttcaaagc gtttccgaaa acgagcgctt ccgaaaatgc aacgcgagct gcgcacatac 
     6061 agctcactgt tcacgtcgca cctatatctg cgtgttgcct gtatatatat atacatgaga 
     6121 agaacggcat agtgcgtgtt tatgcttaaa tgcgtactta tatgcgtcta tttatgtagg 
     6181 atgaaaggta gtctagtacc tcctgtgata ttatcccatt ccatgcgggg tatcgtatgc 
     6241 ttccttcagc actacccttt agctgttcta tatgctgcca ctcctcaatt ggattagtct 
     6301 catccttcaa tgctatcatt tcctttgata ttggatcatc taagaaacca ttattatcat 
     6361 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtc 
//
