LOCUS       p424MET25   6223 bp dsDNA    Circular
DEFINITION  LMBP 4015.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     CDS             complement(3821..4681)
                     /label=AMP
     CDS             468..1142
                     /label=TRP1
     rep_origin      complement(4813..5870)
                     /label=2mu\ori
     promoter        1867..1883
                     /label=T7\promoter
     terminator      complement(1904..2151)
                     /label=CYC1\terminator
     promoter        complement(2623..2639)
                     /label=T3\promoter
     promoter        complement(2703..2786)
                     /label=lac\promoter
     rep_origin      2878..3280
                     /label=pMB1\ori
     promoter        complement(2216..2607)
                     /label=MET25\promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accataaacg acattactat atatataata taggaagcat ttaatagaca gcatcgtaat 
      241 atatgtgtac tttgcagtta tgacgccaga tggcagtagt ggaagatatt ctttattgaa 
      301 aaatagcttg tcaccttacg tacaatcttg atccggagct tttctttttt tgccgattaa 
      361 gaattaattc ggtcgaaaaa agaaaaggag agggccaaga gggagggcat tggtgactat 
      421 tgagcacgtg agtatacgtg attaagcaca caaaggcagc ttggagtatg tctgttatta 
      481 atttcacagg tagttctggt ccattggtga aagtttgcgg cttgcagagc acagaggccg 
      541 cagaatgtgc tctagattcc gatgctgact tgctgggtat tatatgtgtg cccaatagaa 
      601 agagaacaat tgacccggtt attgcaagga aaatttcaag tcttgtaaaa gcatataaaa 
      661 atagttcagg cactccgaaa tacttggttg gcgtgtttcg taatcaacct aaggaggatg 
      721 ttttggctct ggtcaatgat tacggcattg atatcgtcca actgcatgga gatgagtcgt 
      781 ggcaagaata ccaagagttc ctcggtttgc cagttattaa aagactcgta tttccaaaag 
      841 actgcaacat actactcagt gcagcttcac agaaacctca ttcgtttatt cccttgtttg 
      901 attcagaagc aggtgggaca ggtgaacttt tggattggaa ctcgatttct gactgggttg 
      961 gaaggcaaga gagccccgaa agcttacatt ttatgttagc tggtggactg acgccagaaa 
     1021 atgttggtga tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg 
     1081 agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat 
     1141 aggttattac tgagtagtat ttatttaagt attgtttgtg cacttgccta tgcggtgtga 
     1201 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt 
     1261 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa 
     1321 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca 
     1381 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc 
     1441 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg 
     1501 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg 
     1561 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg 
     1621 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg 
     1681 ccgctacagg gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga 
     1741 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga 
     1801 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag 
     1861 cgcgcgtaat acgactcact atagggcgaa ttgggtaccg gccgcaaatt aaagccttcg 
     1921 agcgtcccaa aaccttctca agcaaggttt tcagtataat gttacatgcg tacacgcgtc 
     1981 tgtacagaaa aaaaagaaaa atttgaaata taaataacgt tcttaatact aacataacta 
     2041 taaaaaaata aatagggacc tagacttcag gttgtctaac tccttccttt tcggttagag 
     2101 cggatgtggg gggagggcgt gaatgtaagc gtgacataac taattacatg actcgaggtc 
     2161 gacggtatcg ataagcttga tatcgaattc ctgcagcccg ggggatccac tagttctaga 
     2221 tgtatggatg ggggtaatag aattgtatct atgtatctga cgaccctgta ttacacgaag 
     2281 gaaatagtag atgaaaagac aagagagcaa gaaaaaggaa aaacttcctt tctaacagac 
     2341 gctttactta accttatata tatcttattt ttttcatcga gcgtgttcaa ttggacaagg 
     2401 tgccaccttt tcgacacttc ggttattatg ttacacagtt ttcatgagga tggcgccttg 
     2461 actaacttaa ttagtcattt gccaaccacc acagttcccc aatatcgaca gcttcacgtg 
     2521 ccatttgcca ttttgcgatc atgtgacctc aagagaaaaa gcaaaaaata atgagagcta 
     2581 agggattcga acccttgcat ccgagctcca gcttttgttc cctttagtga gggttaattg 
     2641 cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa 
     2701 ttccacacaa cataggagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga 
     2761 ggtaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 
     2821 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 
     2881 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 
     2941 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 
     3001 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 
     3061 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 
     3121 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 
     3181 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 
     3241 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 
     3301 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 
     3361 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 
     3421 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 
     3481 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 
     3541 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 
     3601 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 
     3661 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 
     3721 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 
     3781 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 
     3841 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 
     3901 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 
     3961 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 
     4021 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 
     4081 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 
     4141 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 
     4201 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 
     4261 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 
     4321 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 
     4381 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 
     4441 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 
     4501 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 
     4561 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 
     4621 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 
     4681 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 
     4741 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 
     4801 aagtgccacc tgaacgaagc atctgtgctt cattttgtag aacaaaaatg caacgcgaga 
     4861 gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa atgcaacgcg 
     4921 aaagcgctat tttaccaacg aagaatctgt gcttcatttt tgtaaaacaa aaatgcaacg 
     4981 cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa cagaaatgca 
     5041 acgcgagagc gctattttac caacaaagaa tctatacttc ttttttgttc tacaaaaatg 
     5101 catcccgaga gcgctatttt tctaacaaag catcttagat tacttttttt ctcctttgtg 
     5161 cgctctataa tgcagtctct tgataacttt ttgcactgta ggtccgttaa ggttagaaga 
     5221 aggctacttt ggtgtctatt ttctcttcca taaaaaaagc ctgactccac ttcccgcgtt 
     5281 tactgattac tagcgaagct gcgggtgcat tttttcaaga taaaggcatc cccgattata 
     5341 ttctataccg atgtggattg cgcatacttt gtgaacagaa agtgatagcg ttgatgattc 
     5401 ttcattggtc agaaaattat gaacggtttc ttctattttg tctctatata ctacgtatag 
     5461 gaaatgttta cattttcgta ttgttttcga ttcactctat gaatagttct tactacaatt 
     5521 tttttgtcta aagagtaata ctagagataa acataaaaaa tgtagaggtc gagtttagat 
     5581 gcaagttcaa ggagcgaaag gtggatgggt aggttatata gggatatagc acagagatat 
     5641 atagcaaaga gatacttttg agcaatgttt gtggaagcgg tattcgcaat attttagtag 
     5701 ctcgttacag tccggtgcgt ttttggtttt ttgaaagtgc gtcttcagag cgcttttggt 
     5761 tttcaaaagc gctctgaagt tcctatactt tctagagaat aggaacttcg gaataggaac 
     5821 ttcaaagcgt ttccgaaaac gagcgcttcc gaaaatgcaa cgcgagctgc gcacatacag 
     5881 ctcactgttc acgtcgcacc tatatctgcg tgttgcctgt atatatatat acatgagaag 
     5941 aacggcatag tgcgtgttta tgcttaaatg cgtacttata tgcgtctatt tatgtaggat 
     6001 gaaaggtagt ctagtacctc ctgtgatatt atcccattcc atgcggggta tcgtatgctt 
     6061 ccttcagcac taccctttag ctgttctata tgctgccact cctcaattgg attagtctca 
     6121 tccttcaatg ctatcatttc ctttgatatt ggatcatatt aagaaaccat tattatcatg 
     6181 acattaacct ataaaaatag gcgtatcacg aggccctttc gtc 
//
