LOCUS p415MET25 6628 bp dsDNA Circular DEFINITION LMBP 4016. COMMENT VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection| COMMENT VNTOAUTHORTEL|0032-9-33-13-843| COMMENT VNTOAUTHOREML|bccm.genecorner@UGent.be| COMMENT VNTOAUTHORWWW|www.genecorner.ugent.be| COMMENT VNTOAUTHORAD1|Ghent University| COMMENT VNTOAUTHORAD2|Technologiepark 927| COMMENT VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| FEATURES Location/Qualifiers rep_origin 6056..6574 /label=CEN6/ARSH4 CDS complement(663..1769) /label=LEU2 CDS complement(5054..5914) /label=AMP promoter 3100..3116 /label=T7\promoter terminator complement(3137..3384) /label=CYC1\terminator promoter complement(3449..3840) /label=MET25\promoter promoter complement(3856..3872) /label=T3\promoter promoter complement(3936..4019) /label=lac\promoter rep_origin 4111..4513 /label=pMB1\ori rep_origin complement(2474..2929) /label=f1\ori ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatcga ctacgtcgta aggccgtttc tgacagagta aaattcttga gggaactttc 241 accattatgg gaaatggttc aagaaggtat tgacttaaac tccatcaaat ggtcaggtca 301 ttgagtgttt tttatttgtt gtattttttt ttttttagag aaaatcctcc aatatcaaat 361 taggaatcgt agtttcatga ttttctgtta cacctaactt tttgtgtggt gccctcctcc 421 ttgtcaatat taatgttaaa gtgcaattct ttttccttat cacgttgagc cattagtatc 481 aatttgctta cctgtattcc tttactatcc tcctttttct ccttcttgat aaatgtatgt 541 agattgcgta tatagtttcg tctaccctat gaacatattc cattttgtaa tttcgtgtcg 601 tttctattat gaatttcatt tataaagttt atgtacaaat atcataaaaa aagagaatct 661 ttttaagcaa ggattttctt aacttcttcg gcgacagcat caccgacttc ggtggtactg 721 ttggaaccac ctaaatcacc agttctgata cctgcatcca aaaccttttt aactgcatct 781 tcaatggcct taccttcttc aggcaagttc aatgacaatt tcaacatcat tgcagcagac 841 aagatagtgg cgatagggtc aaccttattc tttggcaaat ctggagcaga accgtggcat 901 ggttcgtaca aaccaaatgc ggtgttcttg tctggcaaag aggccaagga cgcagatggc 961 aacaaaccca aggaacctgg gataacggag gcttcatcgg agatgatatc accaaacatg 1021 ttgctggtga ttataatacc atttaggtgg gttgggttct taactaggat catggcggca 1081 gaatcaatca attgatgttg aaccttcaat gtagggaatt cgttcttgat ggtttcctcc 1141 acagtttttc tccataatct tgaagaggcc aaaacattag ctttatccaa ggaccaaata 1201 ggcaatggtg gctcatgttg tagggccatg aaagcggcca ttcttgtgat tctttgcact 1261 tctggaacgg tgtattgttc actatcccaa gcgacaccat caccatcgtc ttcctttctc 1321 ttaccaaagt aaatacctcc cactaattct ctgacaacaa cgaagtcagt acctttagca 1381 aattgtggct tgattggaga taagtctaaa agagagtcgg atgcaaagtt acatggtctt 1441 aagttggcgt acaattgaag ttctttacgg atttttagta aaccttgttc aggtctaaca 1501 ctaccggtac cccatttagg accacccaca gcacctaaca aaacggcatc aaccttcttg 1561 gaggcttcca gcgcctcatc tggaagtggg acacctgtag catcgatagc agcaccacca 1621 attaaatgat tttcgaaatc gaacttgaca ttggaacgaa catcagaaat agctttaaga 1681 accttaatgg cttcggctgt gatttcttga ccaacgtggt cacctggcaa aacgacgatc 1741 ttcttagggg cagacatagg ggcagacatt agaatggtat atccttgaaa tatatatata 1801 tattgctgaa atgtaaaagg taagaaaagt tagaaagtaa gacgattgct aaccacctat 1861 tggaaaaaac aataggtcct taaataatat tgtcaacttc aagtattgtg atgcaagcat 1921 ttagtcatga acgcttctct attctatatg aaaagccggt tccggcctct cacctttcct 1981 ttttctccca atttttcagt tgaaaaaggt atatgcgtca ggcgacctct gaaattaaca 2041 aaaaatttcc agtcatcgaa tttgattctg tgcgatagcg cccctgtgtg ttctcgttat 2101 gttgaggaaa aaaataatgg ttgctaagag attcgaactc ttgcatctta cgatacctga 2161 gtattcccac agttaactgc ggtcaagata tttcttgaat caggcgcctt agaccgctcg 2221 gccaaacaac caattacttg ttgagaaata gagtataatt atcctataaa tataacgttt 2281 ttgaacacac atgaacaagg aagtacagga caattgattt tgaagagaat gtggattttg 2341 atgtaattgt tgggattcca tttttaataa ggcaataata ttaggtatgt ggatatacta 2401 gaagttctcc tcgaccgtcg atatgcggtg tgaaataccg cacagatgcg taaggagaaa 2461 ataccgcatc aggaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt 2521 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa 2581 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag 2641 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt 2701 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac 2761 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag 2821 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg 2881 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc gcgccattcg 2941 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc 3001 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc 3061 cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc 3121 gaattgggta ccggccgcaa attaaagcct tcgagcgtcc caaaaccttc tcaagcaagg 3181 ttttcagtat aatgttacat gcgtacacgc gtctgtacag aaaaaaaaga aaaatttgaa 3241 atataaataa cgttcttaat actaacataa ctataaaaaa ataaataggg acctagactt 3301 caggttgtct aactccttcc ttttcggtta gagcggatgt ggggggaggg cgtgaatgta 3361 agcgtgacat aactaattac atgactcgag gtcgacggta tcgataagct tgatatcgaa 3421 ttcctgcagc ccgggggatc cactagttct agatgtatgg atgggggtaa tagaattgta 3481 tctatgtatc tgacgaccct gtattacacg aaggaaatag tagatgaaaa gacaagagag 3541 caagaaaaag gaaaaacttc ctttctaaca gacgctttac ttaaccttat atatatctta 3601 tttttttcat cgagcgtgtt caattggaca aggtgccacc ttttcgacac ttcggttatt 3661 atgttacaca gttttcatga ggatggcgcc ttgactaact taattagtca tttgccaacc 3721 accacagttc cccaatatcg acagcttcac gtgccatttg ccattttgcg atcatgtgac 3781 ctcaagagaa aaagcaaaaa ataatgagag ctaagggatt cgaacccttg catccgagct 3841 ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc 3901 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatagga gccggaagca 3961 taaagtgtaa agcctggggt gcctaatgag tgaggtaact cacattaatt gcgttgcgct 4021 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 4081 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 4141 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 4201 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 4261 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 4321 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 4381 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 4441 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 4501 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 4561 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 4621 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 4681 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag 4741 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 4801 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 4861 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 4921 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 4981 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 5041 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 5101 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 5161 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 5221 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 5281 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 5341 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 5401 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 5461 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 5521 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 5581 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 5641 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 5701 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 5761 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 5821 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 5881 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 5941 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 6001 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgggtcc ttttcatcac 6061 gtgctataaa aataattata atttaaattt tttaatataa atatataaat taaaaataga 6121 aagtaaaaaa agaaattaaa gaaaaaatag tttttgtttt ccgaagatgt aaaagactct 6181 agggggatcg ccaacaaata ctacctttta tcttgctctt cctgctctca ggtattaatg 6241 ccgaattgtt tcatcttgtc tgtgtagaag accacacacg aaaatcctgt gattttacat 6301 tttacttatc gttaatcgaa tgtatatcta tttaatctgc ttttcttgtc taataaatat 6361 atatgtaaag tacgcttttt gttgaaattt tttaaacctt tgtttatttt tttttcttca 6421 ttccgtaact cttctacctt ctttatttac tttctaaaat ccaaatacaa aacataaaaa 6481 taaataaaca cagagtaaat tcccaaatta ttccatcatt aaaagatacg aggcgcgtgt 6541 aagttacagg caagcgatcc gtcctaagaa accattatta tcatgacatt aacctataaa 6601 aataggcgta tcacgaggcc ctttcgtc //