LOCUS       p425MET25   7456 bp dsDNA    Circular
DEFINITION  LMBP 4017.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     promoter        complement(3449..3840)
                     /label=MET25\Promoter
     rep_origin      complement(6046..7103)
                     /label=2mu\ori
     CDS             complement(663..1769)
                     /label=LEU2
     CDS             complement(5054..5914)
                     /label=AMP
     promoter        3100..3116
                     /label=T7\promoter
     terminator      complement(3137..3384)
                     /label=CYC1\terminator
     promoter        complement(3856..3872)
                     /label=T3\promoter
     promoter        complement(3936..4019)
                     /label=lac\promoter
     rep_origin      4111..4513
                     /label=pMB1\ori
     rep_origin      complement(2474..2929)
                     /label=f1\ori
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accatatcga ctacgtcgta aggccgtttc tgacagagta aaattcttga gggaactttc 
      241 accattatgg gaaatgcttc aagaaggtat tgacttaaac tccatcaaat ggtcaggtca 
      301 ttgagtgttt tttatttgtt gtattttttt ttttttagag aaaatcctcc aatatcaaat 
      361 taggaatcgt agtttcatga ttttctgtta cacctaactt tttgtgtggt gccctcctcc 
      421 ttgtcaatat taatgttaaa gtgcaattct ttttccttat cacgttgagc cattagtatc 
      481 aatttgctta cctgtattcc tttactatcc tcctttttct ccttcttgat aaatgtatgt 
      541 agattgcgta tatagtttcg tctaccctat gaacatattc cattttgtaa tttcgtgtcg 
      601 tttctattat gaatttcatt tataaagttt atgtacaaat atcataaaaa aagagaatct 
      661 ttttaagcaa ggattttctt aacttcttcg gcgacagcat caccgacttc ggtggtactg 
      721 ttggaaccac ctaaatcacc agttctgata cctgcatcca aaaccttttt aactgcatct 
      781 tcaatggcct taccttcttc aggcaagttc aatgacaatt tcaacatcat tgcagcagac 
      841 aagatagtgg cgatagggtc aaccttattc tttggcaaat ctggagcaga accgtggcat 
      901 ggttcgtaca aaccaaatgc ggtgttcttg tctggcaaag aggccaagga cgcagatggc 
      961 aacaaaccca aggaacctgg gataacggag gcttcatcgg agatgatatc accaaacatg 
     1021 ttgctggtga ttataatacc atttaggtgg gttgggttct taactaggat catggcggca 
     1081 gaatcaatca attgatgttg aaccttcaat gtagggaatt cgttcttgat ggtttcctcc 
     1141 acagtttttc tccataatct tgaagaggcc aaaagattag ctttatccaa ggaccaaata 
     1201 ggcaatggtg gctcatgttg tagggccatg aaagcggcca ttcttgtgat tctttgcact 
     1261 tctggaacgg tgtattgttc actatcccaa gcgacaccat caccatcgtc ttcctttctc 
     1321 ttaccaaagt aaatacctcc cactaattct ctgacaacaa cgaagtcagt acctttagca 
     1381 aattgtggct tgattggaga taagtctaaa agagagtcgg atgcaaagtt acatggtctt 
     1441 aagttggcgt acaattgaag ttctttacgg atttttagta aaccttgttc aggtctaaca 
     1501 ctaccggtac cccatttagg accagccaca gcacctaaca aaacggcatc aaccttcttg 
     1561 gaggcttcca gcgcctcatc tggaagtggg acacctgtag catcgatagc agcaccacca 
     1621 attaaatgat tttcgaaatc gaacttgaca ttggaacgaa catcagaaat agctttaaga 
     1681 accttaatgg cttcggctgt gatttcttga ccaacgtggt cacctggcaa aacgacgatc 
     1741 ttcttagggg cagacatagg ggcagacatt agaatggtat atccttgaaa tatatatata 
     1801 tattgctgaa atgtaaaagg taagaaaagt tagaaagtaa gacgattgct aaccacctat 
     1861 tggaaaaaac aataggtcct taaataatat tgtcaacttc aagtattgtg atgcaagcat 
     1921 ttagtcatga acgcttctct attctatatg aaaagccggt tccggcctct cacctttcct 
     1981 ttttctccca atttttcagt tgaaaaaggt atatgcgtca ggcgacctct gaaattaaca 
     2041 aaaaatttcc agtcatcgaa tttgattctg tgcgatagcg cccctgtgtg ttctcgttat 
     2101 gttgaggaaa aaaataatgg ttgctaagag attcgaactc ttgcatctta cgatacctga 
     2161 gtattcccac agttaactgc ggtcaagata tttcttgaat caggcgcctt agaccgctcg 
     2221 gccaaacaac caattacttg ttgagaaata gagtataatt atcctataaa tataacgttt 
     2281 ttgaacacac atgaacaagg aagtacagga caattgattt tgaagagaat gtggattttg 
     2341 atgtaattgt tgggattcca tttttaataa ggcaataata ttaggtatgt ggatatacta 
     2401 gaagttctcc tcgaccgtcg atatgcggtg tgaaataccg cacagatgcg taaggagaaa 
     2461 ataccgcatc aggaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt 
     2521 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa 
     2581 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag 
     2641 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt 
     2701 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac 
     2761 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag 
     2821 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg 
     2881 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc gcgccattcg 
     2941 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc 
     3001 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc 
     3061 cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc 
     3121 gaattgggta ccggccgcaa attaaagcct tcgagcgtcc caaaaccttc tcaagcaagg 
     3181 ttttcagtat aatgttacat gcgtacacgc gtctgtacag aaaaaaaaga aaaatttgaa 
     3241 atataaataa cgttcttaat actaacataa ctataaaaaa ataaataggg acctagactt 
     3301 caggttgtct aactccttcc ttttcggtta gagcggatgt ggggggaggg cgtgaatgta 
     3361 agcgtgacat aactaattac atgactcgag gtcgacggta tcgataagct tgatatcgaa 
     3421 ttcctgcagc ccgggggatc cactagttct agatgtatgg atgggggtaa tagaattgta 
     3481 tctatgtatc tgacgaccct gtattacacg aaggaaatag tagatgaaaa gacaagagag 
     3541 caagaaaaag gaaaaacttc ctttctaaca gacgctttac ttaaccttat atatatctta 
     3601 tttttttcat cgagcgtgtt caattggaca aggtgccacc ttttcgacac ttcggttatt 
     3661 atgttacaca gttttcatga ggatggcgcc ttgactaact taattagtca tttgccaacc 
     3721 accacagttc cccaatatcg acagcttcac gtgccatttg ccattttgcg atcatgtgac 
     3781 ctcaagagaa aaagcaaaaa ataatgagag ctaagggatt cgaacccttg catccgagct 
     3841 ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc 
     3901 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatagga gccggaagca 
     3961 taaagtgtaa agcctggggt gcctaatgag tgaggtaact cacattaatt gcgttgcgct 
     4021 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 
     4081 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 
     4141 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 
     4201 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 
     4261 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 
     4321 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 
     4381 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 
     4441 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 
     4501 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 
     4561 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 
     4621 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 
     4681 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag 
     4741 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 
     4801 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 
     4861 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 
     4921 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 
     4981 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 
     5041 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 
     5101 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 
     5161 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 
     5221 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 
     5281 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 
     5341 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 
     5401 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 
     5461 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 
     5521 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 
     5581 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 
     5641 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 
     5701 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 
     5761 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 
     5821 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 
     5881 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 
     5941 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 
     6001 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgaacga agcatctgtg 
     6061 cttcattttg tagaacaaaa atgcaacgcg agagcgctaa tttttcaaac aaagaatctg 
     6121 agctgcattt ttacagaaca gaaatgcaac gcgaaagcgc tattttacca acgaagaatc 
     6181 tgtgcttcat ttttgtaaaa caaaaatgca acgcgagagc gctaattttt caaacaaaga 
     6241 atctgagctg catttttaca gaacagaaat gcaacgcgag agcgctattt taccaacaaa 
     6301 gaatctatac ttcttttttg ttctacaaaa atgcatcccg agagcgctat ttttctaaca 
     6361 aagcatctta gattactttt tttctccttt gtgcgctcta taatgcagtc tcttgataac 
     6421 tttttgcact gtaggtccgt taaggttaga agaaggctac tttggtgtct attttctctt 
     6481 ccataaaaaa agcctgactc cacttcccgc gtttactgat tactagcgaa gctgcgggtg 
     6541 cattttttca agataaaggc atccccgatt atattctata ccgatgtgga ttgcgcatac 
     6601 tttgtgaaca gaaagtgata gcgttgatga ttcttcattg gtcagaaaat tatgaacggt 
     6661 ttcttctatt ttgtctctat atactacgta taggaaatgt ttacattttc gtattgtttt 
     6721 cgattcactc tatgaatagt tcttactaca atttttttgt ctaaagagta atactagaga 
     6781 taaacataaa aaatgtagag gtcgagttta gatgcaagtt caaggagcga aaggtggatg 
     6841 ggtaggttat atagggatat agcacagaga tatatagcaa agagatactt ttgagcaatg 
     6901 tttgtggaag cggtattcgc aatattttag tagctcgtta cagtccggtg cgtttttggt 
     6961 tttttgaaag tgcgtcttca gagcgctttt ggttttcaaa agcgctctga agttcctata 
     7021 ctttctagag aataggaact tcggaatagg aacttcaaag cgtttccgaa aacgagcgct 
     7081 tccgaaaatg caacgcgagc tgcgcacata cagctcactg ttcacgtcgc acctatatct 
     7141 gcgtgttgcc tgtatatata tatacatgag aagaacggca tagtgcgtgt ttatgcttaa 
     7201 atgcgtactt atatgcgtct atttatgtag gatgaaaggt agtctagtac ctcctgtgat 
     7261 attatcccat tccatgcggg gtatcgtatg cttccttcag cactaccctt tagctgttct 
     7321 atatgctgcc actcctcaat tggattagtc tcatccttca atgctatcat ttcctttgat 
     7381 attggatcat actaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 
     7441 acgaggccct ttcgtc 
//
