LOCUS p416MET25 5505 bp dsDNA Circular DEFINITION LMBP 4018. COMMENT VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection| COMMENT VNTOAUTHORTEL|0032-9-33-13-843| COMMENT VNTOAUTHOREML|bccm.genecorner@UGent.be| COMMENT VNTOAUTHORWWW|www.genecorner.ugent.be| COMMENT VNTOAUTHORAD1|Ghent University| COMMENT VNTOAUTHORAD2|Technologiepark 927| COMMENT VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| FEATURES Location/Qualifiers rep_origin 4933..5451 /label=CEN6/ARSH4 CDS complement(3931..4791) /label=AMP CDS 417..1220 /label=URA3 promoter 1978..1993 /label=T7\promoter terminator complement(2014..2261) /label=CYC1\terminator promoter complement(2733..2749) /label=T3\promoter promoter complement(2813..2896) /label=lac\promoter rep_origin 2988..3390 /label=pMB1\ori rep_origin complement(1351..1806) /label=f1\ori promoter complement(2326..2717) /label=MET25\promoter ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc 241 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg 301 agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc 361 cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt 421 cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat 481 ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca 541 aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg 601 tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg 661 ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca 721 aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac 781 acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa 841 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg 901 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct 961 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac 1021 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg 1081 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa 1141 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa 1201 gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac 1261 aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac 1321 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat 1381 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa 1441 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca 1501 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg 1561 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta 1621 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg 1681 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa 1741 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg 1801 gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg 1861 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg 1921 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat 1981 acgactcact atagggcgaa ttgggtaccg gccgcaaatt aaagccttcg agcgtcccaa 2041 aaccttctca agcaaggttt tcagtataat gttacatgcg tacacgcgtc tgtacagaaa 2101 aaaaagaaaa atttgaaata taaataacgt tcttaatact aacataacta taaaaaaata 2161 aatagggacc tagacttcag gttgtctaac tccttccttt tcggttagag cggatgtggg 2221 gggagggcgt gaatgtaagc gtgacataac taattacatg actcgaggtc gacggtatcg 2281 ataagcttga tatcgaattc ctgcagcccg ggggatccac tagttctaga tgtatggatg 2341 ggggtaatag aattgtatct atgtatctga cgaccctgta ttacacgaag gaaatagtag 2401 atgaaaagac aagagagcaa gaaaaaggaa aaacttcctt tctaacagac gctttactta 2461 accttatata tatcttattt ttttcatcga gcgtgttcaa ttggacaagg tgccaccttt 2521 tcgacacttc ggttattatg ttacacagtt ttcatgagga tggcgccttg actaacttaa 2581 ttagtcattt gccaaccacc acagttcccc aatatcgaca gcttcacgtg ccatttgcca 2641 ttttgcgatc atgtgacctc aagagaaaaa gcaaaaaata atgagagcta agggattcga 2701 acccttgcat ccgagctcca gcttttgttc cctttagtga gggttaattg cgcgcttggc 2761 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 2821 cataggagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga ggtaactcac 2881 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 2941 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 3001 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 3061 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 3121 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 3181 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 3241 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 3301 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 3361 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 3421 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 3481 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 3541 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 3601 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 3661 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 3721 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 3781 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 3841 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 3901 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 3961 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 4021 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 4081 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 4141 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 4201 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 4261 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 4321 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 4381 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 4441 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 4501 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 4561 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 4621 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 4681 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 4741 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 4801 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 4861 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 4921 tgggtccttt tcatcacgtg ctataaaaat aattataatt taaatttttt aatataaata 4981 tataaattaa aaatagaaag taaaaaaaga aattaaagaa aaaatagttt ttgttttccg 5041 aagatgtaaa agactctagg gggatcgcca acaaatacta ccttttatct tgctcttcct 5101 gctctcaggt attaatgccg aattgtttca tcttgtctgt gtagaagacc acacacgaaa 5161 atcctgtgat tttacatttt acttatcgtt aatcgaatgt atatctattt aatctgcttt 5221 tcttgtctaa taaatatata tgtaaagtac gctttttgtt gaaatttttt aaacctttgt 5281 ttattttttt ttcttcattc cgtaactctt ctaccttctt tatttacttt ctaaaatcca 5341 aatacaaaac ataaaaataa ataaacacag agtaaattcc caaattattc catcattaaa 5401 agatacgagg cgcgtgtaag ttacaggcaa gcgatccgtc ctaagaaacc attattatca 5461 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtc //