LOCUS       p426MET25   6333 bp dsDNA    Circular
DEFINITION  LMBP 4019.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     rep_origin      complement(4923..5980)
                     /label=2mu\ori
     promoter        complement(2326..2717)
                     /label=MET25\promoter
     promoter        1977..1993
                     /label=T7\promoter
     terminator      complement(2014..2261)
                     /label=CYC1\terminator
     promoter        complement(2733..2749)
                     /label=T3\promoter
     promoter        complement(2813..2896)
                     /label=lac\promoter
     rep_origin      2988..3390
                     /label=pMB1\ori
     rep_origin      complement(1351..1806)
                     /label=f1\ori
     CDS             complement(3931..4791)
                     /label=AMP
     CDS             417..1220
                     /label=URA3
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc 
      241 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg 
      301 agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc 
      361 cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt 
      421 cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat 
      481 ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca 
      541 aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg 
      601 tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg 
      661 ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca 
      721 aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac 
      781 acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa 
      841 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg 
      901 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct 
      961 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac 
     1021 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg 
     1081 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa 
     1141 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa 
     1201 gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac 
     1261 aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac 
     1321 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat 
     1381 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa 
     1441 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca 
     1501 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg 
     1561 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta 
     1621 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg 
     1681 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa 
     1741 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg 
     1801 gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg 
     1861 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg 
     1921 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat 
     1981 acgactcact atagggcgaa ttgggtaccg gccgcaaatt aaagccttcg agcgtcccaa 
     2041 aaccttctca agcaaggttt tcagtataat gttacatgcg tacacgcgtc tgtacagaaa 
     2101 aaaaagaaaa atttgaaata taaataacgt tcttaatact aacataacta taaaaaaata 
     2161 aatagggacc tagacttcag gttgtctaac tccttccttt tcggttagag cggatgtggg 
     2221 gggagggcgt gaatgtaagc gtgacataac taattacatg actcgaggtc gacggtatcg 
     2281 ataagcttga tatcgaattc ctgcagcccg ggggatccac tagttctaga tgtatggatg 
     2341 ggggtaatag aattgtatct atgtatctga cgaccctgta ttacacgaag gaaatagtag 
     2401 atgaaaagac aagagagcaa gaaaaaggaa aaacttcctt tctaacagac gctttactta 
     2461 accttatata tatcttattt ttttcatcga gcgtgttcaa ttggacaagg tgccaccttt 
     2521 tcgacacttc ggttattatg ttacacagtt ttcatgagga tggcgccttg actaacttaa 
     2581 ttagtcattt gccaaccacc acagttcccc aatatcgaca gcttcacgtg ccatttgcca 
     2641 ttttgcgatc atgtgacctc aagagaaaaa gcaaaaaata atgagagcta agggattcga 
     2701 acccttgcat ccgagctcca gcttttgttc cctttagtga gggttaattg cgcgcttggc 
     2761 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 
     2821 cataggagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga ggtaactcac 
     2881 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 
     2941 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 
     3001 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 
     3061 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 
     3121 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 
     3181 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 
     3241 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 
     3301 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 
     3361 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 
     3421 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 
     3481 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 
     3541 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 
     3601 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 
     3661 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 
     3721 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 
     3781 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 
     3841 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 
     3901 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 
     3961 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 
     4021 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 
     4081 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 
     4141 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 
     4201 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 
     4261 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 
     4321 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 
     4381 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 
     4441 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 
     4501 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 
     4561 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 
     4621 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 
     4681 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 
     4741 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 
     4801 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 
     4861 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 
     4921 tgaacgaagc atctgtgctt cattttgtag aacaaaaatg caacgcgaga gcgctaattt 
     4981 ttcaaacaaa gaatctgagc tgcattttta cagaacagaa atgcaacgcg aaagcgctat 
     5041 tttaccaacg aagaatctgt gcttcatttt tgtaaaacaa aaatgcaacg cgagagcgct 
     5101 aatttttcaa acaaagaatc tgagctgcat ttttacagaa cagaaatgca acgcgagagc 
     5161 gctattttac caacaaagaa tctatacttc ttttttgttc tacaaaaatg catcccgaga 
     5221 gcgctatttt tctaacaaag catcttagat tacttttttt ctcctttgtg cgctctataa 
     5281 tgcagtctct tgataacttt ttgcactgta ggtccgttaa ggttagaaga aggctacttt 
     5341 ggtgtctatt ttctcttcca taaaaaaagc ctgactccac ttcccgcgtt tactgattac 
     5401 tagcgaagct gcgggtgcat tttttcaaga taaaggcatc cccgattata ttctataccg 
     5461 atgtggattg cgcatacttt gtgaacagaa agtgatagcg ttgatgattc ttcattggtc 
     5521 agaaaattat gaacggtttc ttctattttg tctctatata ctacgtatag gaaatgttta 
     5581 cattttcgta ttgttttcga ttcactctat gaatagttct tactacaatt tttttgtcta 
     5641 aagagtaata ctagagataa acataaaaaa tgtagaggtc gagtttagat gcaagttcaa 
     5701 ggagcgaaag gtggatgggt aggttatata gggatatagc acagagatat atagcaaaga 
     5761 gatacttttg agcaatgttt gtggaagcgg tattcgcaat attttagtag ctcgttacag 
     5821 tccggtgcgt ttttggtttt ttgaaagtgc gtcttcagag cgcttttggt tttcaaaagc 
     5881 gctctgaagt tcctatactt tctagagaat aggaacttcg gaataggaac ttcaaagcgt 
     5941 ttccgaaaac gagcgcttcc gaaaatgcaa cgcgagctgc gcacatacag ctcactgttc 
     6001 acgtcgcacc tatatctgcg tgttgcctgt atatatatat acatgagaag aacggcatag 
     6061 tgcgtgttta tgcttaaatg cgtacttata tgcgtctatt tatgtaggat gaaaggtagt 
     6121 ctagtacctc ctgtgatatt atcccattcc atgcggggta tcgtatgctt ccttcagcac 
     6181 taccctttag ctgttctata tgctgccact cctcaattgg attagtctca tccttcaatg 
     6241 ctatcatttc ctttgatatt ggatcatact aagaaaccat tattatcatg acattaacct 
     6301 ataaaaatag gcgtatcacg aggccctttc gtc 
//
