LOCUS       p424GAL1    6301 bp dsDNA    Circular
DEFINITION  LMBP 4023.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     rep_origin      complement(4891..5948)
                     /label=2mu\ori
     CDS             468..1142
                     /label=TRP1
     CDS             complement(3899..4759)
                     /label=AMP
     promoter        1867..1883
                     /label=T7\promoter
     terminator      complement(1904..2151)
                     /label=CYC1\terminator
     promoter        complement(2282..2665)
                     /label=GAL1\promoter
     promoter        complement(2701..2717)
                     /label=T3\promoter
     promoter        complement(2781..2864)
                     /label=lac\promoter
     rep_origin      2956..3358
                     /label=pMB1\ori
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accataaacg acattactat atatataata taggaagcat ttaatagaca gcatcgtaat 
      241 atatgtgtac tttgcagtta tgacgccaga tggcagtagt ggaagatatt ctttattgaa 
      301 aaatagcttg tcaccttacg tacaatcttg atccggagct tttctttttt tgccgattaa 
      361 gaattaattc ggtcgaaaaa agaaaaggag agggccaaga gggagggcat tggtgactat 
      421 tgagcacgtg agtatacgtg attaagcaca caaaggcagc ttggagtatg tctgttatta 
      481 atttcacagg tagttctggt ccattggtga aagtttgcgg cttgcagagc acagaggccg 
      541 cagaatgtgc tctagattcc gatgctgact tgctgggtat tatatgtgtg cccaatagaa 
      601 agagaacaat tgacccggtt attgcaagga aaatttcaag tcttgtaaaa gcatataaaa 
      661 atagttcagg cactccgaaa tacttggttg gcgtgtttcg taatcaacct aaggaggatg 
      721 ttttggctct ggtcaatgat tacggcattg atatcgtcca actgcatgga gatgagtcgt 
      781 ggcaagaata ccaagagttc ctcggtttgc cagttattaa aagactcgta tttccaaaag 
      841 actgcaacat actactcagt gcagcttcac agaaacctca ttcgtttatt cccttgtttg 
      901 attcagaagc aggtgggaca ggtgaacttt tggattggaa ctcgatttct gactgggttg 
      961 gaaggcaaga gagccccgaa agcttacatt ttatgttagc tggtggactg acgccagaaa 
     1021 atgttggtga tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg 
     1081 agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat 
     1141 aggttattac tgagtagtat ttatttaagt attgtttgtg cacttgccta tgcggtgtga 
     1201 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt 
     1261 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa 
     1321 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca 
     1381 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc 
     1441 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg 
     1501 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg 
     1561 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg 
     1621 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg 
     1681 ccgctacagg gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga 
     1741 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga 
     1801 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag 
     1861 cgcgcgtaat acgactcact atagggcgaa ttgggtaccg gccgcaaatt aaagccttcg 
     1921 agcgtcccaa aaccttctca agcaaggttt tcagtataat gttacatgcg tacacgcgtc 
     1981 tgtacagaaa aaaaagaaaa atttgaaata taaataacgt tcttaatact aacataacta 
     2041 taaaaaaata aatagggacc tagacttcag gttgtctaac tccttccttt tcggttagag 
     2101 cggatgtggg gggagggcgt gaatgtaagc gtgacataac taattacatg actcgaggtc 
     2161 gacggtatcg ataagcttga tatcgaattc ctgcagcccg ggggatccac tagttctaga 
     2221 tatagttttt tctccttgac gttaaagtat agaggtatat taacaatttt ttgttgatac 
     2281 ttttattaca tttgaataag aagtaataca aaccgaaaat gttgaaagta ttagttaaag 
     2341 tggttatgca gtttttgcat ttatatatct gttaatagat caaaaatcat cgcttcgctg 
     2401 attaattacc ccagaaataa ggctaaaaaa ctaatcgcat tatcatccta tggttgttaa 
     2461 tttgattcgt tcatttgaag gtttgtgggg ccaggttact gccaattttt cctcttcata 
     2521 accataaaag ctagtattgt agaatcttta ttgttcggag cagtgcggcg cgaggcacat 
     2581 ctgcgtttca ggaacgcgac cggtgaagac gaggacgcac ggaggagagt cttccttcgg 
     2641 agggctgtca cccgctcggc ggcttctaat ccgtacttca gagctccagc ttttgttccc 
     2701 tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa 
     2761 attgttatcc gctcacaatt ccacacaaca taggagccgg aagcataaag tgtaaagcct 
     2821 ggggtgccta atgagtgagg taactcacat taattgcgtt gcgctcactg cccgctttcc 
     2881 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 
     2941 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 
     3001 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 
     3061 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 
     3121 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 
     3181 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 
     3241 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 
     3301 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 
     3361 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 
     3421 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 
     3481 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 
     3541 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 
     3601 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 
     3661 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 
     3721 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 
     3781 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 
     3841 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 
     3901 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 
     3961 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 
     4021 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 
     4081 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 
     4141 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 
     4201 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 
     4261 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 
     4321 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 
     4381 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 
     4441 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 
     4501 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 
     4561 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 
     4621 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 
     4681 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 
     4741 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 
     4801 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 
     4861 cgcgcacatt tccccgaaaa gtgccacctg aacgaagcat ctgtgcttca ttttgtagaa 
     4921 caaaaatgca acgcgagagc gctaattttt caaacaaaga atctgagctg catttttaca 
     4981 gaacagaaat gcaacgcgaa agcgctattt taccaacgaa gaatctgtgc ttcatttttg 
     5041 taaaacaaaa atgcaacgcg agagcgctaa tttttcaaac aaagaatctg agctgcattt 
     5101 ttacagaaca gaaatgcaac gcgagagcgc tattttacca acaaagaatc tatacttctt 
     5161 ttttgttcta caaaaatgca tcccgagagc gctatttttc taacaaagca tcttagatta 
     5221 ctttttttct cctttgtgcg ctctataatg cagtctcttg ataacttttt gcactgtagg 
     5281 tccgttaagg ttagaagaag gctactttgg tgtctatttt ctcttccata aaaaaagcct 
     5341 gactccactt cccgcgttta ctgattacta gcgaagctgc gggtgcattt tttcaagata 
     5401 aaggcatccc cgattatatt ctataccgat gtggattgcg catactttgt gaacagaaag 
     5461 tgatagcgtt gatgattctt cattggtcag aaaattatga acggtttctt ctattttgtc 
     5521 tctatatact acgtatagga aatgtttaca ttttcgtatt gttttcgatt cactctatga 
     5581 atagttctta ctacaatttt tttgtctaaa gagtaatact agagataaac ataaaaaatg 
     5641 tagaggtcga gtttagatgc aagttcaagg agcgaaaggt ggatgggtag gttatatagg 
     5701 gatatagcac agagatatat agcaaagaga tacttttgag caatgtttgt ggaagcggta 
     5761 ttcgcaatat tttagtagct cgttacagtc cggtgcgttt ttggtttttt gaaagtgcgt 
     5821 cttcagagcg cttttggttt tcaaaagcgc tctgaagttc ctatactttc tagagaatag 
     5881 gaacttcgga ataggaactt caaagcgttt ccgaaaacga gcgcttccga aaatgcaacg 
     5941 cgagctgcgc acatacagct cactgttcac gtcgcaccta tatctgcgtg ttgcctgtat 
     6001 atatatatac atgagaagaa cggcatagtg cgtgtttatg cttaaatgcg tacttatatg 
     6061 cgtctattta tgtaggatga aaggtagtct agtacctcct gtgatattat cccattccat 
     6121 gcggggtatc gtatgcttcc ttcagcacta ccctttagct gttctatatg ctgccactcc 
     6181 tcaattggat tagtctcatc cttcaatgct atcatttcct ttgatattgg atcatattaa 
     6241 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt 
     6301 c 
//
