LOCUS       p416GAL1    5583 bp dsDNA    Circular
DEFINITION  LMBP 4026.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     rep_origin      5011..5529
                     /label=CEN6/ARSH4
     CDS             complement(4009..4869)
                     /label=AMP
     CDS             417..1220
                     /label=URA3
     promoter        1977..1993
                     /label=T7\promoter
     terminator      complement(2014..2261)
                     /label=CYC1\terminator
     promoter        complement(2392..2775)
                     /label=GAL1\promoter
     promoter        complement(2811..2827)
                     /label=T3\promoter
     promoter        complement(2891..2974)
                     /label=lac\promoter
     rep_origin      3066..3468
                     /label=pMB1\ori
     rep_origin      complement(1351..1806)
                     /label=f1\ori
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc 
      241 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg 
      301 agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc 
      361 cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt 
      421 cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat 
      481 ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca 
      541 aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg 
      601 tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg 
      661 ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca 
      721 aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac 
      781 acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa 
      841 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg 
      901 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct 
      961 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac 
     1021 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg 
     1081 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa 
     1141 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa 
     1201 gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac 
     1261 aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac 
     1321 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat 
     1381 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa 
     1441 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca 
     1501 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg 
     1561 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta 
     1621 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg 
     1681 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa 
     1741 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg 
     1801 gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg 
     1861 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg 
     1921 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat 
     1981 acgactcact atagggcgaa ttgggtaccg gccgcaaatt aaagccttcg agcgtcccaa 
     2041 aaccttctca agcaaggttt tcagtataat gttacatgcg tacacgcgtc tgtacagaaa 
     2101 aaaaagaaaa atttgaaata taaataacgt tcttaatact aacataacta taaaaaaata 
     2161 aatagggacc tagacttcag gttgtctaac tccttccttt tcggttagag cggatgtggg 
     2221 gggagggcgt gaatgtaagc gtgacataac taattacatg actcgaggtc gacggtatcg 
     2281 ataagcttga tatcgaattc ctgcagcccg ggggatccac tagttctaga tatagttttt 
     2341 tctccttgac gttaaagtat agaggtatat taacaatttt ttgttgatac ttttattaca 
     2401 tttgaataag aagtaataca aaccgaaaat gttgaaagta ttagttaaag tggttatgca 
     2461 gtttttgcat ttatatatct gttaatagat caaaaatcat cgcttcgctg attaattacc 
     2521 ccagaaataa ggctaaaaaa ctaatcgcat tatcatccta tggttgttaa tttgattcgt 
     2581 tcatttgaag gtttgtgggg ccaggttact gccaattttt cctcttcata accataaaag 
     2641 ctagtattgt agaatcttta ttgttcggag cagtgcggcg cgaggcacat ctgcgtttca 
     2701 ggaacgcgac cggtgaagac gaggacgcac ggaggagagt cttccttcgg agggctgtca 
     2761 cccgctcggc ggcttctaat ccgtacttca gagctccagc ttttgttccc tttagtgagg 
     2821 gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 
     2881 gctcacaatt ccacacaaca taggagccgg aagcataaag tgtaaagcct ggggtgccta 
     2941 atgagtgagg taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 
     3001 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 
     3061 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 
     3121 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 
     3181 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 
     3241 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 
     3301 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 
     3361 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 
     3421 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 
     3481 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 
     3541 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 
     3601 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 
     3661 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 
     3721 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 
     3781 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 
     3841 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 
     3901 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 
     3961 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 
     4021 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 
     4081 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 
     4141 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 
     4201 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 
     4261 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 
     4321 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 
     4381 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 
     4441 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 
     4501 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 
     4561 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 
     4621 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 
     4681 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 
     4741 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 
     4801 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 
     4861 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 
     4921 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 
     4981 tccccgaaaa gtgccacctg ggtccttttc atcacgtgct ataaaaataa ttataattta 
     5041 aattttttaa tataaatata taaattaaaa atagaaagta aaaaaagaaa ttaaagaaaa 
     5101 aatagttttt gttttccgaa gatgtaaaag actctagggg gatcgccaac aaatactacc 
     5161 ttttatcttg ctcttcctgc tctcaggtat taatgccgaa ttgtttcatc ttgtctgtgt 
     5221 agaagaccac acacgaaaat cctgtgattt tacattttac ttatcgttaa tcgaatgtat 
     5281 atctatttaa tctgcttttc ttgtctaata aatatatatg taaagtacgc tttttgttga 
     5341 aattttttaa acctttgttt attttttttt cttcattccg taactcttct accttcttta 
     5401 tttactttct aaaatccaaa tacaaaacat aaaaataaat aaacacagag taaattccca 
     5461 aattattcca tcattaaaag atacgaggcg cgtgtaagtt acaggcaagc gatccgtcct 
     5521 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc 
     5581 gtc 
//
