LOCUS       p414GALL    5443 bp dsDNA    Circular
DEFINITION  LMBP 4030.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     rep_origin      4871..5389
                     /label=CEN6/ARSH4
     CDS             complement(3869..4729)
                     /label=AMP
     CDS             468..1142
                     /label=TRP1
     promoter        1867..1883
                     /label=T7\promoter
     terminator      complement(1904..2151)
                     /label=CYC1\terminator
     promoter        complement(2282..2655)
                     /label=GALL\promoter
     promoter        complement(2671..2687)
                     /label=T3\promoter
     promoter        complement(2751..2834)
                     /label=lac\promoter
     rep_origin      2926..3328
                     /label=pMB1\ori
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accataaacg acattactat atatataata taggaagcat ttaatagaca gcatcgtaat 
      241 atatgtgtac tttgcagtta tgacgccaga tggcagtagt ggaagatatt ctttattgaa 
      301 aaatagcttg tcaccttacg tacaatcttg atccggagct tttctttttt tgccgattaa 
      361 gaattaattc ggtcgaaaaa agaaaaggag agggccaaga gggagggcat tggtgactat 
      421 tgagcacgtg agtatacgtg attaagcaca caaaggcagc ttggagtatg tctgttatta 
      481 atttcacagg tagttctggt ccattggtga aagtttgcgg cttgcagagc acagaggccg 
      541 cagaatgtgc tctagattcc gatgctgact tgctgggtat tatatgtgtg cccaatagaa 
      601 agagaacaat tgacccggtt attgcaagga aaatttcaag tcttgtaaaa gcatataaaa 
      661 atagttcagg cactccgaaa tacttggttg gcgtgtttcg taatcaacct aaggaggatg 
      721 ttttggctct ggtcaatgat tacggcattg atatcgtcca actgcatgga gatgagtcgt 
      781 ggcaagaata ccaagagttc ctcggtttgc cagttattaa aagactcgta tttccaaaag 
      841 actgcaacat actactcagt gcagcttcac agaaacctca ttcgtttatt cccttgtttg 
      901 attcagaagc aggtgggaca ggtgaacttt tggattggaa ctcgatttct gactgggttg 
      961 gaaggcaaga gagccccgaa agcttacatt ttatgttagc tggtggactg acgccagaaa 
     1021 atgttggtga tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg 
     1081 agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat 
     1141 aggttattac tgagtagtat ttatttaagt attgtttgtg cacttgccta tgcggtgtga 
     1201 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt 
     1261 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa 
     1321 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca 
     1381 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc 
     1441 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg 
     1501 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg 
     1561 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg 
     1621 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg 
     1681 ccgctacagg gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga 
     1741 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga 
     1801 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag 
     1861 cgcgcgtaat acgactcact atagggcgaa ttgggtaccg gccgcaaatt aaagccttcg 
     1921 agcgtcccaa aaccttctca agcaaggttt tcagtataat gttacatgcg tacacgcgtc 
     1981 tgtacagaaa aaaaagaaaa atttgaaata taaataacgt tcttaatact aacataacta 
     2041 taaaaaaata aatagggacc tagacttcag gttgtctaac tccttccttt tcggttagag 
     2101 cggatgtggg gggagggcgt gaatgtaagc gtgacataac taattacatg actcgaggtc 
     2161 gacggtatcg ataagcttga tatcgaattc ctgcagcccg ggggatccac tagttctaga 
     2221 tatagttttt tctccttgac gttaaagtat agaggtatat taacaatttt ttgttgatac 
     2281 ttttattaca tttgaataag aagtaataca aaccgaaaat gttgaaagta ttagttaaag 
     2341 tggttatgca gtttttgcat ttatatatct gttaatagat caaaaatcat cgcttcgctg 
     2401 attaattacc ccagaaataa ggctaaaaaa ctaatcgcat tatcatccta tggttgttaa 
     2461 tttgattcgt tcatttgaag gtttgtgggg ccaggttact gccaattttt cctcttcata 
     2521 accataaaag ctagtattgt agaatcttta ttgttcggag cagtgcggcg cgaggcacat 
     2581 ctgcgtttca ggaacgcgac cggtgaagac gaggacgcac ggaggagagt cttccttcgg 
     2641 agggctgtca gagctccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt 
     2701 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 
     2761 taggagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagg taactcacat 
     2821 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 
     2881 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 
     2941 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 
     3001 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 
     3061 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 
     3121 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 
     3181 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 
     3241 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 
     3301 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 
     3361 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 
     3421 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 
     3481 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 
     3541 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 
     3601 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 
     3661 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 
     3721 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 
     3781 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 
     3841 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 
     3901 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 
     3961 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 
     4021 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 
     4081 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 
     4141 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 
     4201 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 
     4261 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 
     4321 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 
     4381 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 
     4441 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 
     4501 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 
     4561 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 
     4621 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 
     4681 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 
     4741 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 
     4801 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 
     4861 ggtccttttc atcacgtgct ataaaaataa ttataattta aattttttaa tataaatata 
     4921 taaattaaaa atagaaagta aaaaaagaaa ttaaagaaaa aatagttttt gttttccgaa 
     4981 gatgtaaaag actctagggg gatcgccaac aaatactacc ttttatcttg ctcttcctgc 
     5041 tctcaggtat taatgccgaa ttgtttcatc ttgtctgtgt agaagaccac acacgaaaat 
     5101 cctgtgattt tacattttac ttatcgttaa tcgaatgtat atctatttaa tctgcttttc 
     5161 ttgtctaata aatatatatg taaagtacgc tttttgttga aattttttaa acctttgttt 
     5221 attttttttt cttcattccg taactcttct accttcttta tttactttct aaaatccaaa 
     5281 tacaaaacat aaaaataaat aaacacagag taaattccca aattattcca tcattaaaag 
     5341 atacgaggcg cgtgtaagtt acaggcaagc gatccgtcct aagaaaccat tattatcatg 
     5401 acattaacct ataaaaatag gcgtatcacg aggccctttc gtc 
//
