LOCUS p425GALL 7504 bp dsDNA Circular DEFINITION LMBP 4033. COMMENT VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection| COMMENT VNTOAUTHORTEL|0032-9-33-13-843| COMMENT VNTOAUTHOREML|bccm.genecorner@UGent.be| COMMENT VNTOAUTHORWWW|www.genecorner.ugent.be| COMMENT VNTOAUTHORAD1|Ghent University| COMMENT VNTOAUTHORAD2|Technologiepark 927| COMMENT VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| FEATURES Location/Qualifiers rep_origin 2474..2930 /label=f1\ori rep_origin complement(6094..7151) /label=2mu\ori promoter 3100..3116 /label=T7\promoter CDS complement(663..1769) /label=LEU2 CDS complement(5102..5962) /label=AMP terminator 3137..3384 /label=CYC1\terminator promoter complement(3515..3888) /label=GALL\promoter promoter complement(3904..3920) /label=T3\promoter promoter complement(3984..4067) /label=lac\promoter rep_origin 4159..4561 /label=pMB1\ori rep_origin complement(2922..2929) /label=f1\ori ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatcga ctacgtcgta aggccgtttc tgacagagta aaattcttga gggaactttc 241 accattatgg gaaatgcttc aagaaggtat tgacttaaac tccatcaaat ggtcaggtca 301 ttgagtgttt tttatttgtt gtattttttt ttttttagag aaaatcctcc aatatcaaat 361 taggaatcgt agtttcatga ttttctgtta cacctaactt tttgtgtggt gccctcctcc 421 ttgtcaatat taatgttaaa gtgcaattct ttttccttat cacgttgagc cattagtatc 481 aatttgctta cctgtattcc tttactatcc tcctttttct ccttcttgat aaatgtatgt 541 agattgcgta tatagtttcg tctaccctat gaacatattc cattttgtaa tttcgtgtcg 601 tttctattat gaatttcatt tataaagttt atgtacaaat atcataaaaa aagagaatct 661 ttttaagcaa ggattttctt aacttcttcg gcgacagcat caccgacttc ggtggtactg 721 ttggaaccac ctaaatcacc agttctgata cctgcatcca aaaccttttt aactgcatct 781 tcaatggcct taccttcttc aggcaagttc aatgacaatt tcaacatcat tgcagcagac 841 aagatagtgg cgatagggtc aaccttattc tttggcaaat ctggagcaga accgtggcat 901 ggttcgtaca aaccaaatgc ggtgttcttg tctggcaaag aggccaagga cgcagatggc 961 aacaaaccca aggaacctgg gataacggag gcttcatcgg agatgatatc accaaacatg 1021 ttgctggtga ttataatacc atttaggtgg gttgggttct taactaggat catggcggca 1081 gaatcaatca attgatgttg aaccttcaat gtagggaatt cgttcttgat ggtttcctcc 1141 acagtttttc tccataatct tgaagaggcc aaaagattag ctttatccaa ggaccaaata 1201 ggcaatggtg gctcatgttg tagggccatg aaagcggcca ttcttgtgat tctttgcact 1261 tctggaacgg tgtattgttc actatcccaa gcgacaccat caccatcgtc ttcctttctc 1321 ttaccaaagt aaatacctcc cactaattct ctgacaacaa cgaagtcagt acctttagca 1381 aattgtggct tgattggaga taagtctaaa agagagtcgg atgcaaagtt acatggtctt 1441 aagttggcgt acaattgaag ttctttacgg atttttagta aaccttgttc aggtctaaca 1501 ctaccggtac cccatttagg accagccaca gcacctaaca aaacggcatc aaccttcttg 1561 gaggcttcca gcgcctcatc tggaagtggg acacctgtag catcgatagc agcaccacca 1621 attaaatgat tttcgaaatc gaacttgaca ttggaacgaa catcagaaat agctttaaga 1681 accttaatgg cttcggctgt gatttcttga ccaacgtggt cacctggcaa aacgacgatc 1741 ttcttagggg cagacatagg ggcagacatt agaatggtat atccttgaaa tatatatata 1801 tattgctgaa atgtaaaagg taagaaaagt tagaaagtaa gacgattgct aaccacctat 1861 tggaaaaaac aataggtcct taaataatat tgtcaacttc aagtattgtg atgcaagcat 1921 ttagtcatga acgcttctct attctatatg aaaagccggt tccggcctct cacctttcct 1981 ttttctccca atttttcagt tgaaaaaggt atatgcgtca ggcgacctct gaaattaaca 2041 aaaaatttcc agtcatcgaa tttgattctg tgcgatagcg cccctgtgtg ttctcgttat 2101 gttgaggaaa aaaataatgg ttgctaagag attcgaactc ttgcatctta cgatacctga 2161 gtattcccac agttaactgc ggtcaagata tttcttgaat caggcgcctt agaccgctcg 2221 gccaaacaac caattacttg ttgagaaata gagtataatt atcctataaa tataacgttt 2281 ttgaacacac atgaacaagg aagtacagga caattgattt tgaagagaat gtggattttg 2341 atgtaattgt tgggattcca tttttaataa ggcaataata ttaggtatgt ggatatacta 2401 gaagttctcc tcgaccgtcg atatgcggtg tgaaataccg cacagatgcg taaggagaaa 2461 ataccgcatc aggaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt 2521 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa 2581 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag 2641 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt 2701 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac 2761 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag 2821 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg 2881 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc gcgccattcg 2941 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc 3001 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc 3061 cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc 3121 gaattgggta ccggccgcaa attaaagcct tcgagcgtcc caaaaccttc tcaagcaagg 3181 ttttcagtat aatgttacat gcgtacacgc gtctgtacag aaaaaaaaga aaaatttgaa 3241 atataaataa cgttcttaat actaacataa ctataaaaaa ataaataggg acctagactt 3301 caggttgtct aactccttcc ttttcggtta gagcggatgt ggggggaggg cgtgaatgta 3361 agcgtgacat aactaattac atgactcgag gtcgacggta tcgataagct tgatatcgaa 3421 ttcctgcagc ccgggggatc cactagttct agatatagtt ttttctcctt gacgttaaag 3481 tatagaggta tattaacaat tttttgttga tacttttatt acatttgaat aagaagtaat 3541 acaaaccgaa aatgttgaaa gtattagtta aagtggttat gcagtttttg catttatata 3601 tctgttaata gatcaaaaat catcgcttcg ctgattaatt accccagaaa taaggctaaa 3661 aaactaatcg cattatcatc ctatggttgt taatttgatt cgttcatttg aaggtttgtg 3721 gggccaggtt actgccaatt tttcctcttc ataaccataa aagctagtat tgtagaatct 3781 ttattgttcg gagcagtgcg gcgcgaggca catctgcgtt tcaggaacgc gaccggtgaa 3841 gacgaggacg cacggaggag agtcttcctt cggagggctg tcagagctcc agcttttgtt 3901 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt 3961 gaaattgtta tccgctcaca attccacaca acataggagc cggaagcata aagtgtaaag 4021 cctggggtgc ctaatgagtg aggtaactca cattaattgc gttgcgctca ctgcccgctt 4081 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 4141 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 4201 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 4261 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 4321 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 4381 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 4441 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 4501 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 4561 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 4621 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 4681 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 4741 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 4801 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 4861 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 4921 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 4981 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 5041 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 5101 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 5161 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 5221 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 5281 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 5341 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 5401 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 5461 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 5521 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 5581 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 5641 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 5701 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 5761 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 5821 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 5881 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 5941 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 6001 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 6061 ttccgcgcac atttccccga aaagtgccac ctgaacgaag catctgtgct tcattttgta 6121 gaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa agaatctgag ctgcattttt 6181 acagaacaga aatgcaacgc gaaagcgcta ttttaccaac gaagaatctg tgcttcattt 6241 ttgtaaaaca aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca 6301 tttttacaga acagaaatgc aacgcgagag cgctatttta ccaacaaaga atctatactt 6361 cttttttgtt ctacaaaaat gcatcccgag agcgctattt ttctaacaaa gcatcttaga 6421 ttactttttt tctcctttgt gcgctctata atgcagtctc ttgataactt tttgcactgt 6481 aggtccgtta aggttagaag aaggctactt tggtgtctat tttctcttcc ataaaaaaag 6541 cctgactcca cttcccgcgt ttactgatta ctagcgaagc tgcgggtgca ttttttcaag 6601 ataaaggcat ccccgattat attctatacc gatgtggatt gcgcatactt tgtgaacaga 6661 aagtgatagc gttgatgatt cttcattggt cagaaaatta tgaacggttt cttctatttt 6721 gtctctatat actacgtata ggaaatgttt acattttcgt attgttttcg attcactcta 6781 tgaatagttc ttactacaat ttttttgtct aaagagtaat actagagata aacataaaaa 6841 atgtagaggt cgagtttaga tgcaagttca aggagcgaaa ggtggatggg taggttatat 6901 agggatatag cacagagata tatagcaaag agatactttt gagcaatgtt tgtggaagcg 6961 gtattcgcaa tattttagta gctcgttaca gtccggtgcg tttttggttt tttgaaagtg 7021 cgtcttcaga gcgcttttgg ttttcaaaag cgctctgaag ttcctatact ttctagagaa 7081 taggaacttc ggaataggaa cttcaaagcg tttccgaaaa cgagcgcttc cgaaaatgca 7141 acgcgagctg cgcacataca gctcactgtt cacgtcgcac ctatatctgc gtgttgcctg 7201 tatatatata tacatgagaa gaacggcata gtgcgtgttt atgcttaaat gcgtacttat 7261 atgcgtctat ttatgtagga tgaaaggtag tctagtacct cctgtgatat tatcccattc 7321 catgcggggt atcgtatgct tccttcagca ctacccttta gctgttctat atgctgccac 7381 tcctcaattg gattagtctc atccttcaat gctatcattt cctttgatat tggatcatac 7441 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt 7501 cgtc //