LOCUS       p415GALS    6676 bp dsDNA    Circular
DEFINITION  LMBP 4040.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     CDS             complement(663..1769)
                     /label=LEU2
     CDS             complement(5102..5962)
                     /label=AMP
     promoter        3100..3116
                     /label=T7\promoter
     terminator      complement(3137..3384)
                     /label=CYC1\terminator
     promoter        complement(3904..3920)
                     /label=T3\promoter
     promoter        complement(3984..4067)
                     /label=lac\promoter
     rep_origin      4159..4561
                     /label=pMB1\ori
     rep_origin      complement(2474..2929)
                     /label=f1\ori
     promoter        complement(3515..3888)
                     /label=GALS\promoter
     rep_origin      6104..6622
                     /label=CEN6/ARSH4
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accatatcga ctacgtcgta aggccgtttc tgacagagta aaattcttga gggaactttc 
      241 accattatgg gaaatggttc aagaaggtat tgacttaaac tccatcaaat ggtcaggtca 
      301 ttgagtgttt tttatttgtt gtattttttt ttttttagag aaaatcctcc aatatcaaat 
      361 taggaatcgt agtttcatga ttttctgtta cacctaactt tttgtgtggt gccctcctcc 
      421 ttgtcaatat taatgttaaa gtgcaattct ttttccttat cacgttgagc cattagtatc 
      481 aatttgctta cctgtattcc tttactatcc tcctttttct ccttcttgat aaatgtatgt 
      541 agattgcgta tatagtttcg tctaccctat gaacatattc cattttgtaa tttcgtgtcg 
      601 tttctattat gaatttcatt tataaagttt atgtacaaat atcataaaaa aagagaatct 
      661 ttttaagcaa ggattttctt aacttcttcg gcgacagcat caccgacttc ggtggtactg 
      721 ttggaaccac ctaaatcacc agttctgata cctgcatcca aaaccttttt aactgcatct 
      781 tcaatggcct taccttcttc aggcaagttc aatgacaatt tcaacatcat tgcagcagac 
      841 aagatagtgg cgatagggtc aaccttattc tttggcaaat ctggagcaga accgtggcat 
      901 ggttcgtaca aaccaaatgc ggtgttcttg tctggcaaag aggccaagga cgcagatggc 
      961 aacaaaccca aggaacctgg gataacggag gcttcatcgg agatgatatc accaaacatg 
     1021 ttgctggtga ttataatacc atttaggtgg gttgggttct taactaggat catggcggca 
     1081 gaatcaatca attgatgttg aaccttcaat gtagggaatt cgttcttgat ggtttcctcc 
     1141 acagtttttc tccataatct tgaagaggcc aaaacattag ctttatccaa ggaccaaata 
     1201 ggcaatggtg gctcatgttg tagggccatg aaagcggcca ttcttgtgat tctttgcact 
     1261 tctggaacgg tgtattgttc actatcccaa gcgacaccat caccatcgtc ttcctttctc 
     1321 ttaccaaagt aaatacctcc cactaattct ctgacaacaa cgaagtcagt acctttagca 
     1381 aattgtggct tgattggaga taagtctaaa agagagtcgg atgcaaagtt acatggtctt 
     1441 aagttggcgt acaattgaag ttctttacgg atttttagta aaccttgttc aggtctaaca 
     1501 ctaccggtac cccatttagg accacccaca gcacctaaca aaacggcatc aaccttcttg 
     1561 gaggcttcca gcgcctcatc tggaagtggg acacctgtag catcgatagc agcaccacca 
     1621 attaaatgat tttcgaaatc gaacttgaca ttggaacgaa catcagaaat agctttaaga 
     1681 accttaatgg cttcggctgt gatttcttga ccaacgtggt cacctggcaa aacgacgatc 
     1741 ttcttagggg cagacatagg ggcagacatt agaatggtat atccttgaaa tatatatata 
     1801 tattgctgaa atgtaaaagg taagaaaagt tagaaagtaa gacgattgct aaccacctat 
     1861 tggaaaaaac aataggtcct taaataatat tgtcaacttc aagtattgtg atgcaagcat 
     1921 ttagtcatga acgcttctct attctatatg aaaagccggt tccggcctct cacctttcct 
     1981 ttttctccca atttttcagt tgaaaaaggt atatgcgtca ggcgacctct gaaattaaca 
     2041 aaaaatttcc agtcatcgaa tttgattctg tgcgatagcg cccctgtgtg ttctcgttat 
     2101 gttgaggaaa aaaataatgg ttgctaagag attcgaactc ttgcatctta cgatacctga 
     2161 gtattcccac agttaactgc ggtcaagata tttcttgaat caggcgcctt agaccgctcg 
     2221 gccaaacaac caattacttg ttgagaaata gagtataatt atcctataaa tataacgttt 
     2281 ttgaacacac atgaacaagg aagtacagga caattgattt tgaagagaat gtggattttg 
     2341 atgtaattgt tgggattcca tttttaataa ggcaataata ttaggtatgt ggatatacta 
     2401 gaagttctcc tcgaccgtcg atatgcggtg tgaaataccg cacagatgcg taaggagaaa 
     2461 ataccgcatc aggaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt 
     2521 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa 
     2581 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag 
     2641 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt 
     2701 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac 
     2761 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag 
     2821 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg 
     2881 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc gcgccattcg 
     2941 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc 
     3001 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc 
     3061 cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc 
     3121 gaattgggta ccggccgcaa attaaagcct tcgagcgtcc caaaaccttc tcaagcaagg 
     3181 ttttcagtat aatgttacat gcgtacacgc gtctgtacag aaaaaaaaga aaaatttgaa 
     3241 atataaataa cgttcttaat actaacataa ctataaaaaa ataaataggg acctagactt 
     3301 caggttgtct aactccttcc ttttcggtta gagcggatgt ggggggaggg cgtgaatgta 
     3361 agcgtgacat aactaattac atgactcgag gtcgacggta tcgataagct tgatatcgaa 
     3421 ttcctgcagc ccgggggatc cactagttct agatatagtt ttttctcctt gacgttaaag 
     3481 tatagaggta tattaacaat tttttgttga tacttttatt acatttgaat aagaagtaat 
     3541 acaaaccgaa aatgttgaaa gtattagtta aagtggttat gcagtttttg catttatata 
     3601 tctgttaata gatcaaaaat catcgcttcg ctgattaatt accccagaaa taaggctaaa 
     3661 aaactaatcg cattatcatc ctatggttgt taatttgatt cgttcatttg aaggtttgtg 
     3721 gggccaggtt actgccaatt tttcctcttc ataaccataa aagctagtat tgtagaatct 
     3781 ttattgttcg gagcagtgcg gcgcgaggca catctgcgtt tcaggaacgc gaccggtgaa 
     3841 gacgaggacg cacggaggag agtcttcctt cggagggctg tcagagctcc agcttttgtt 
     3901 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt 
     3961 gaaattgtta tccgctcaca attccacaca acataggagc cggaagcata aagtgtaaag 
     4021 cctggggtgc ctaatgagtg aggtaactca cattaattgc gttgcgctca ctgcccgctt 
     4081 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 
     4141 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 
     4201 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 
     4261 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 
     4321 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 
     4381 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 
     4441 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 
     4501 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 
     4561 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 
     4621 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 
     4681 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 
     4741 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 
     4801 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 
     4861 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 
     4921 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 
     4981 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 
     5041 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 
     5101 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 
     5161 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 
     5221 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 
     5281 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 
     5341 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 
     5401 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 
     5461 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 
     5521 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 
     5581 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 
     5641 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 
     5701 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 
     5761 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 
     5821 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 
     5881 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 
     5941 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 
     6001 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 
     6061 ttccgcgcac atttccccga aaagtgccac ctgggtcctt ttcatcacgt gctataaaaa 
     6121 taattataat ttaaattttt taatataaat atataaatta aaaatagaaa gtaaaaaaag 
     6181 aaattaaaga aaaaatagtt tttgttttcc gaagatgtaa aagactctag ggggatcgcc 
     6241 aacaaatact accttttatc ttgctcttcc tgctctcagg tattaatgcc gaattgtttc 
     6301 atcttgtctg tgtagaagac cacacacgaa aatcctgtga ttttacattt tacttatcgt 
     6361 taatcgaatg tatatctatt taatctgctt ttcttgtcta ataaatatat atgtaaagta 
     6421 cgctttttgt tgaaattttt taaacctttg tttatttttt tttcttcatt ccgtaactct 
     6481 tctaccttct ttatttactt tctaaaatcc aaatacaaaa cataaaaata aataaacaca 
     6541 gagtaaattc ccaaattatt ccatcattaa aagatacgag gcgcgtgtaa gttacaggca 
     6601 agcgatccgt cctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 
     6661 acgaggccct ttcgtc 
//
