LOCUS       p426GALS    6381 bp dsDNA    Circular
DEFINITION  LMBP 4043.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     rep_origin      complement(4971..6028)
                     /label=2mu\ori
     CDS             417..1220
                     /label=URA3
     CDS             complement(3979..4839)
                     /label=AMP
     promoter        1977..1993
                     /label=T7\promoter
     terminator      complement(2014..2261)
                     /label=CYC1\terminator
     promoter        complement(2781..2797)
                     /label=T3\promoter
     promoter        complement(2861..2944)
                     /label=lac\promoter
     rep_origin      3036..3438
                     /label=pMB1\ori
     rep_origin      complement(1351..1806)
                     /label=f1\ori
     promoter        complement(2392..2765)
                     /label=GALS\promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc 
      241 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg 
      301 agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc 
      361 cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt 
      421 cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat 
      481 ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca 
      541 aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg 
      601 tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg 
      661 ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca 
      721 aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac 
      781 acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa 
      841 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg 
      901 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct 
      961 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac 
     1021 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg 
     1081 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa 
     1141 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa 
     1201 gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac 
     1261 aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac 
     1321 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat 
     1381 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa 
     1441 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca 
     1501 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg 
     1561 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta 
     1621 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg 
     1681 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa 
     1741 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg 
     1801 gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg 
     1861 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg 
     1921 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat 
     1981 acgactcact atagggcgaa ttgggtaccg gccgcaaatt aaagccttcg agcgtcccaa 
     2041 aaccttctca agcaaggttt tcagtataat gttacatgcg tacacgcgtc tgtacagaaa 
     2101 aaaaagaaaa atttgaaata taaataacgt tcttaatact aacataacta taaaaaaata 
     2161 aatagggacc tagacttcag gttgtctaac tccttccttt tcggttagag cggatgtggg 
     2221 gggagggcgt gaatgtaagc gtgacataac taattacatg actcgaggtc gacggtatcg 
     2281 ataagcttga tatcgaattc ctgcagcccg ggggatccac tagttctaga tatagttttt 
     2341 tctccttgac gttaaagtat agaggtatat taacaatttt ttgttgatac ttttattaca 
     2401 tttgaataag aagtaataca aaccgaaaat gttgaaagta ttagttaaag tggttatgca 
     2461 gtttttgcat ttatatatct gttaatagat caaaaatcat cgcttcgctg attaattacc 
     2521 ccagaaataa ggctaaaaaa ctaatcgcat tatcatccta tggttgttaa tttgattcgt 
     2581 tcatttgaag gtttgtgggg ccaggttact gccaattttt cctcttcata accataaaag 
     2641 ctagtattgt agaatcttta ttgttcggag cagtgcggcg cgaggcacat ctgcgtttca 
     2701 ggaacgcgac cggtgaagac gaggacgcac ggaggagagt cttccttcgg agggctgtca 
     2761 gagctccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc 
     2821 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca taggagccgg 
     2881 aagcataaag tgtaaagcct ggggtgccta atgagtgagg taactcacat taattgcgtt 
     2941 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg 
     3001 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 
     3061 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 
     3121 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 
     3181 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 
     3241 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 
     3301 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 
     3361 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 
     3421 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 
     3481 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 
     3541 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 
     3601 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 
     3661 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 
     3721 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 
     3781 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 
     3841 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 
     3901 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 
     3961 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 
     4021 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 
     4081 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 
     4141 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 
     4201 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 
     4261 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc 
     4321 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 
     4381 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 
     4441 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 
     4501 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 
     4561 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag 
     4621 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 
     4681 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 
     4741 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 
     4801 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 
     4861 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 
     4921 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg aacgaagcat 
     4981 ctgtgcttca ttttgtagaa caaaaatgca acgcgagagc gctaattttt caaacaaaga 
     5041 atctgagctg catttttaca gaacagaaat gcaacgcgaa agcgctattt taccaacgaa 
     5101 gaatctgtgc ttcatttttg taaaacaaaa atgcaacgcg agagcgctaa tttttcaaac 
     5161 aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgagagcgc tattttacca 
     5221 acaaagaatc tatacttctt ttttgttcta caaaaatgca tcccgagagc gctatttttc 
     5281 taacaaagca tcttagatta ctttttttct cctttgtgcg ctctataatg cagtctcttg 
     5341 ataacttttt gcactgtagg tccgttaagg ttagaagaag gctactttgg tgtctatttt 
     5401 ctcttccata aaaaaagcct gactccactt cccgcgttta ctgattacta gcgaagctgc 
     5461 gggtgcattt tttcaagata aaggcatccc cgattatatt ctataccgat gtggattgcg 
     5521 catactttgt gaacagaaag tgatagcgtt gatgattctt cattggtcag aaaattatga 
     5581 acggtttctt ctattttgtc tctatatact acgtatagga aatgtttaca ttttcgtatt 
     5641 gttttcgatt cactctatga atagttctta ctacaatttt tttgtctaaa gagtaatact 
     5701 agagataaac ataaaaaatg tagaggtcga gtttagatgc aagttcaagg agcgaaaggt 
     5761 ggatgggtag gttatatagg gatatagcac agagatatat agcaaagaga tacttttgag 
     5821 caatgtttgt ggaagcggta ttcgcaatat tttagtagct cgttacagtc cggtgcgttt 
     5881 ttggtttttt gaaagtgcgt cttcagagcg cttttggttt tcaaaagcgc tctgaagttc 
     5941 ctatactttc tagagaatag gaacttcgga ataggaactt caaagcgttt ccgaaaacga 
     6001 gcgcttccga aaatgcaacg cgagctgcgc acatacagct cactgttcac gtcgcaccta 
     6061 tatctgcgtg ttgcctgtat atatatatac atgagaagaa cggcatagtg cgtgtttatg 
     6121 cttaaatgcg tacttatatg cgtctattta tgtaggatga aaggtagtct agtacctcct 
     6181 gtgatattat cccattccat gcggggtatc gtatgcttcc ttcagcacta ccctttagct 
     6241 gttctatatg ctgccactcc tcaattggat tagtctcatc cttcaatgct atcatttcct 
     6301 ttgatattgg atcatactaa gaaaccatta ttatcatgac attaacctat aaaaataggc 
     6361 gtatcacgag gccctttcgt c 
//
