LOCUS       pRS414                  4788 bp    DNA     circular     18-APR-2019
DEFINITION  LMBP 4058.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4058
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 18-APR-2019
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDBDATE|-12776410|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pRS414|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark-Zwijnaarde 71|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTREPLTYPE|Phage
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     VNTEXTCHREPL|Yeast
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pRS414" 0 4788 
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COMMENT        (CObList) -1 "") 
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COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
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COMMENT         "HindIII" "KpnI" "NotI" "SacI" "SacII" "SmaI" "XhoI") 
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COMMENT       (CMolPar 0 0 0 0 4294967295 1 4788 0 0 0 0 0 0 0 0) (CStringList) 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT        #1=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 1943 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 2 753 0 1939 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "HincII" "gtyrac" 4 0 1 1918 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "HindIII" "aagctt" 2 0 2 981 0 1931 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "ApaI" "gggccc" 6 0 1 1905 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 1961 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "ClaI" "atcgat" 3 0 1 1926 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 1899 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 1980 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "SacI" "gagctc" 6 0 1 2001 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 1 1992 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "SmaI" "cccggg" 4 0 1 1957 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 1910 0 0 "")) 
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COMMENT        #15=(CFSignal (CObList) "lacZ fragment, incl. lacZalpha" 4 0 1 1698 2057 
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COMMENT        #16=(CFSignal (CObList) "f1 ori" 33 0 1 1241 1696 0 (CStringList) 
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COMMENT        #17=(CFSignal (CObList) "T7 promoter" 30 0 0 1867 1883 0 (CStringList) 
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COMMENT        #18=(CFSignal (CObList) "T3 promoter" 30 0 1 2016 2032 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2016..2032)") 
COMMENT        #19=(CFSignal (CObList) "lac promoter" 30 0 1 2096 2179 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2096..2179)") 
COMMENT        #20=(CFSignal (CObList) "5' UTR of lacZ, incl. RBS" 32 0 1 2058 2095 0 
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COMMENT        #21=(CFSignal (CObList) "pMB1 ori" 33 0 1 2271 2673 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2271..2673)") 
COMMENT        #22=(CFSignal (CObList) "ampicillin resistance" 4 0 1 3214 4074 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(3214..4074)") 
COMMENT        #23=(CFSignal (CObList) "CEN6/ARSH4 ori" 33 0 0 4210 4734 0 
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COMMENT                          "DNA Plasmid Phage 'pRS414'" 1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 
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COMMENT                          "Creation Date: ? (no data)" 1) 
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COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
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COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "TEL: 0032-9-33-13-843  " 1) 
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COMMENT                          "EMAIL: <a href=\"mailto:bccm.genecorner@UGent.be\">bccm.genecorner@UGent.be</a>" 
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COMMENT                          "WWW: <a href=\"http://www.genecorner.ugent.be\">www.genecorner.ugent.be</a>" 
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FEATURES             Location/Qualifiers
     CDS             468..1142
                     /vntifkey="4"
                     /label=TRP1
     CDS             complement(1698..2057)
                     /vntifkey="4"
                     /label=lacZ\fragment,\incl.\lacZalpha
     rep_origin      complement(1241..1696)
                     /vntifkey="33"
                     /label=f1\ori
     promoter        1867..1883
                     /vntifkey="30"
                     /label=T7\promoter
     promoter        complement(2016..2032)
                     /vntifkey="30"
                     /label=T3\promoter
     promoter        complement(2096..2179)
                     /vntifkey="30"
                     /label=lac\promoter
     RBS             complement(2058..2095)
                     /vntifkey="32"
                     /label=5'\UTR\of\lacZ,\incl.\RBS
     rep_origin      complement(2271..2673)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(3214..4074)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      4210..4734
                     /vntifkey="33"
                     /label=CEN6/ARSH4\ori
BASE COUNT     1307 a      1053 c      1153 g      1275 t 
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accataaacg acattactat atatataata taggaagcat ttaatagaca gcatcgtaat 
      241 atatgtgtac tttgcagtta tgacgccaga tggcagtagt ggaagatatt ctttattgaa 
      301 aaatagcttg tcaccttacg tacaatcttg atccggagct tttctttttt tgccgattaa 
      361 gaattaattc ggtcgaaaaa agaaaaggag agggccaaga gggagggcat tggtgactat 
      421 tgagcacgtg agtatacgtg attaagcaca caaaggcagc ttggagtatg tctgttatta 
      481 atttcacagg tagttctggt ccattggtga aagtttgcgg cttgcagagc acagaggccg 
      541 cagaatgtgc tctagattcc gatgctgact tgctgggtat tatatgtgtg cccaatagaa 
      601 agagaacaat tgacccggtt attgcaagga aaatttcaag tcttgtaaaa gcatataaaa 
      661 atagttcagg cactccgaaa tacttggttg gcgtgtttcg taatcaacct aaggaggatg 
      721 ttttggctct ggtcaatgat tacggcattg atatcgtcca actgcatgga gatgagtcgt 
      781 ggcaagaata ccaagagttc ctcggtttgc cagttattaa aagactcgta tttccaaaag 
      841 actgcaacat actactcagt gcagcttcac agaaacctca ttcgtttatt cccttgtttg 
      901 attcagaagc aggtgggaca ggtgaacttt tggattggaa ctcgatttct gactgggttg 
      961 gaaggcaaga gagccccgaa agcttacatt ttatgttagc tggtggactg acgccagaaa 
     1021 atgttggtga tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg 
     1081 agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat 
     1141 aggttattac tgagtagtat ttatttaagt attgtttgtg cacttgccta tgcggtgtga 
     1201 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt 
     1261 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa 
     1321 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca 
     1381 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc 
     1441 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg 
     1501 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg 
     1561 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg 
     1621 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg 
     1681 ccgctacagg gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga 
     1741 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga 
     1801 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag 
     1861 cgcgcgtaat acgactcact atagggcgaa ttgggtaccg ggccccccct cgaggtcgac 
     1921 ggtatcgata agcttgatat cgaattcctg cagcccgggg gatccactag ttctagagcg 
     1981 gccgccaccg cggtggagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt 
     2041 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 
     2101 caacatagga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgaggtaact 
     2161 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 
     2221 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 
     2281 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 
     2341 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 
     2401 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 
     2461 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 
     2521 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 
     2581 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 
     2641 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 
     2701 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 
     2761 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 
     2821 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 
     2881 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 
     2941 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 
     3001 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 
     3061 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 
     3121 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 
     3181 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 
     3241 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 
     3301 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 
     3361 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 
     3421 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 
     3481 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 
     3541 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 
     3601 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 
     3661 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 
     3721 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 
     3781 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 
     3841 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 
     3901 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 
     3961 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 
     4021 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 
     4081 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 
     4141 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 
     4201 acctgggtcc ttttcatcac gtgctataaa aataattata atttaaattt tttaatataa 
     4261 atatataaat taaaaataga aagtaaaaaa agaaattaaa gaaaaaatag tttttgtttt 
     4321 ccgaagatgt aaaagactct agggggatcg ccaacaaata ctacctttta tcttgctctt 
     4381 cctgctctca ggtattaatg ccgaattgtt tcatcttgtc tgtgtagaag accacacacg 
     4441 aaaatcctgt gattttacat tttacttatc gttaatcgaa tgtatatcta tttaatctgc 
     4501 ttttcttgtc taataaatat atatgtaaag tacgcttttt gttgaaattt tttaaacctt 
     4561 tgtttatttt tttttcttca ttccgtaact cttctacctt ctttatttac tttctaaaat 
     4621 ccaaatacaa aacataaaaa taaataaaca cagagtaaat tcccaaatta ttccatcatt 
     4681 aaaagatacg aggcgcgtgt aagttacagg caagcgatcc gtcctaagaa accattatta 
     4741 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtc 
//
