ID pRS414 standard; circular DNA; ; 4788 BP. DT 18-APR-2019 DE LMBP 4058. CC Accession number: LMBP 4058 CC (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium) CC Last modified: 18-APR-2019 CC This file is created by Vector NTI CC http://www.invitrogen.com/ CC ORIGDB|GenBank CC VNTDBDATE|-12776410| CC LSOWNER| CC VNTAUTHORNAME|Demo User| CC VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTOAUTHORTEL|0032-9-33-13-843| CC VNTOAUTHOREML|bccm.genecorner@UGent.be| CC VNTOAUTHORWWW|www.genecorner.ugent.be| CC VNTOAUTHORAD1|Ghent University| CC VNTOAUTHORAD2|Technologiepark-Zwijnaarde 71| CC VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC VNTREPLTYPE|Plasmid CC VNTREPLTYPE|Phage CC VNTEXTCHREPL|Bacteria CC VNTEXTCHREPL|Yeast FH Key Location/Qualifiers FH FT CDS 468..1142 FT /vntifkey="4" FT /label=TRP1 FT CDS complement(1698..2057) FT /vntifkey="4" FT /label=lacZ\fragment,\incl.\lacZalpha FT rep_origin complement(1241..1696) FT /vntifkey="33" FT /label=f1\ori FT promoter 1867..1883 FT /vntifkey="30" FT /label=T7\promoter FT promoter complement(2016..2032) FT /vntifkey="30" FT /label=T3\promoter FT promoter complement(2096..2179) FT /vntifkey="30" FT /label=lac\promoter FT RBS complement(2058..2095) FT /vntifkey="32" FT /label=5'\UTR\of\lacZ,\incl.\RBS FT rep_origin complement(2271..2673) FT /vntifkey="33" FT /label=pMB1\ori FT CDS complement(3214..4074) FT /vntifkey="4" FT /label=ampicillin\resistance FT rep_origin 4210..4734 FT /vntifkey="33" FT /label=CEN6/ARSH4\ori SQ Sequence 4788 BP; 1307 A; 1053 C; 1153 G; 1275 t; tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180 accataaacg acattactat atatataata taggaagcat ttaatagaca gcatcgtaat 240 atatgtgtac tttgcagtta tgacgccaga tggcagtagt ggaagatatt ctttattgaa 300 aaatagcttg tcaccttacg tacaatcttg atccggagct tttctttttt tgccgattaa 360 gaattaattc ggtcgaaaaa agaaaaggag agggccaaga gggagggcat tggtgactat 420 tgagcacgtg agtatacgtg attaagcaca caaaggcagc ttggagtatg tctgttatta 480 atttcacagg tagttctggt ccattggtga aagtttgcgg cttgcagagc acagaggccg 540 cagaatgtgc tctagattcc gatgctgact tgctgggtat tatatgtgtg cccaatagaa 600 agagaacaat tgacccggtt attgcaagga aaatttcaag tcttgtaaaa gcatataaaa 660 atagttcagg cactccgaaa tacttggttg gcgtgtttcg taatcaacct aaggaggatg 720 ttttggctct ggtcaatgat tacggcattg atatcgtcca actgcatgga gatgagtcgt 780 ggcaagaata ccaagagttc ctcggtttgc cagttattaa aagactcgta tttccaaaag 840 actgcaacat actactcagt gcagcttcac agaaacctca ttcgtttatt cccttgtttg 900 attcagaagc aggtgggaca ggtgaacttt tggattggaa ctcgatttct gactgggttg 960 gaaggcaaga gagccccgaa agcttacatt ttatgttagc tggtggactg acgccagaaa 1020 atgttggtga tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg 1080 agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat 1140 aggttattac tgagtagtat ttatttaagt attgtttgtg cacttgccta tgcggtgtga 1200 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt 1260 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa 1320 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca 1380 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc 1440 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg 1500 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg 1560 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg 1620 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg 1680 ccgctacagg gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga 1740 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga 1800 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag 1860 cgcgcgtaat acgactcact atagggcgaa ttgggtaccg ggccccccct cgaggtcgac 1920 ggtatcgata agcttgatat cgaattcctg cagcccgggg gatccactag ttctagagcg 1980 gccgccaccg cggtggagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt 2040 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 2100 caacatagga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgaggtaact 2160 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 2220 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 2280 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 2340 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 2400 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 2460 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 2520 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 2580 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 2640 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 2700 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 2760 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 2820 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 2880 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 2940 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 3000 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 3060 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 3120 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 3180 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 3240 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 3300 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 3360 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 3420 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 3480 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 3540 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 3600 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 3660 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 3720 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 3780 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 3840 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 3900 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 3960 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 4020 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 4080 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 4140 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 4200 acctgggtcc ttttcatcac gtgctataaa aataattata atttaaattt tttaatataa 4260 atatataaat taaaaataga aagtaaaaaa agaaattaaa gaaaaaatag tttttgtttt 4320 ccgaagatgt aaaagactct agggggatcg ccaacaaata ctacctttta tcttgctctt 4380 cctgctctca ggtattaatg ccgaattgtt tcatcttgtc tgtgtagaag accacacacg 4440 aaaatcctgt gattttacat tttacttatc gttaatcgaa tgtatatcta tttaatctgc 4500 ttttcttgtc taataaatat atatgtaaag tacgcttttt gttgaaattt tttaaacctt 4560 tgtttatttt tttttcttca ttccgtaact cttctacctt ctttatttac tttctaaaat 4620 ccaaatacaa aacataaaaa taaataaaca cagagtaaat tcccaaatta ttccatcatt 4680 aaaagatacg aggcgcgtgt aagttacagg caagcgatcc gtcctaagaa accattatta 4740 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtc 4788 //