LOCUS       pRS415                  6021 bp    DNA     circular     18-APR-2019
DEFINITION  LMBP 4059.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4059
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 18-APR-2019
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDBDATE|-12776422|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pRS415|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark-Zwijnaarde 71|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTREPLTYPE|Phage
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     VNTEXTCHREPL|Yeast
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pRS415" 0 6021 
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COMMENT        (CObList) -1 "") 
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COMMENT         "PstI" "SacI" "SacII" "SmaI" "SpeI" "XbaI" "XhoI") 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT        #1=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 1117 0 3176 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 2 1008 0 3172 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "HincII" "gtyrac" 4 0 4 861 0 1895 0 2175 0 
COMMENT            3151 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 3164 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 3194 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 3213 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 3186 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SacI" "gagctc" 6 0 1 3234 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 1 3225 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "SmaI" "cccggg" 4 0 1 3190 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "SpeI" "actagt" 2 0 1 3200 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 3206 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 3143 0 0 "")) 
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COMMENT        #15=(CFSignal (CObList) "lacZ fragment, incl. lacZalpha" 4 0 1 2931 3290 
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COMMENT             (CStringList) 1 1 1 1 "2049..2065") 
COMMENT        #18=(CFSignal (CObList) "T3 promoter" 30 0 1 3249 3265 0 (CStringList) 
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COMMENT        #19=(CFSignal (CObList) "lac promoter" 30 0 1 3329 3412 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2278..2361)") 
COMMENT        #20=(CFSignal (CObList) "5' UTR of lacZ, incl. RBS" 32 0 1 3291 3328 0 
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COMMENT        #21=(CFSignal (CObList) "pMB1 ori" 33 0 1 3504 3906 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2453..2855)") 
COMMENT        #22=(CFSignal (CObList) "ampicillin resistance" 4 0 1 4447 5307 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(3396..4256)") 
COMMENT        #23=(CFSignal (CObList) "CEN6/ARSH4 ori" 33 0 0 5443 5967 0 
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COMMENT                          "DNA Plasmid Phage 'pRS415'" 1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 
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COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Creation Date: ? (no data)" 1) 
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COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6021 bp" 1) 
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COMMENT                          "TEL: 0032-9-33-13-843  " 1) 
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COMMENT                          "EMAIL: <a href=\"mailto:bccm.genecorner@UGent.be\">bccm.genecorner@UGent.be</a>" 
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FEATURES             Location/Qualifiers
     CDS             complement(663..1757)
                     /vntifkey="4"
                     /label=LEU2
     CDS             complement(2931..3290)
                     /vntifkey="4"
                     /label=lacZ\fragment,\incl.\lacZalpha
     rep_origin      complement(2474..2929)
                     /vntifkey="33"
                     /label=f1\ori
     promoter        3100..3116
                     /vntifkey="30"
                     /label=T7\promoter
     promoter        complement(3249..3265)
                     /vntifkey="30"
                     /label=T3\promoter
     promoter        complement(3329..3412)
                     /vntifkey="30"
                     /label=lac\promoter
     RBS             complement(3291..3328)
                     /vntifkey="32"
                     /label=5'\UTR\of\lacZ,\incl.\RBS
     rep_origin      complement(3504..3906)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(4447..5307)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      5443..5967
                     /vntifkey="33"
                     /label=CEN6/ARSH4\ori
BASE COUNT     1663 a      1336 c      1339 g      1683 t 
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accatatcga ctacgtcgta aggccgtttc tgacagagta aaattcttga gggaactttc 
      241 accattatgg gaaatggttc aagaaggtat tgacttaaac tccatcaaat ggtcaggtca 
      301 ttgagtgttt tttatttgtt gtattttttt ttttttagag aaaatcctcc aatatcaaat 
      361 taggaatcgt agtttcatga ttttctgtta cacctaactt tttgtgtggt gccctcctcc 
      421 ttgtcaatat taatgttaaa gtgcaattct ttttccttat cacgttgagc cattagtatc 
      481 aatttgctta cctgtattcc tttactatcc tcctttttct ccttcttgat aaatgtatgt 
      541 agattgcgta tatagtttcg tctaccctat gaacatattc cattttgtaa tttcgtgtcg 
      601 tttctattat gaatttcatt tataaagttt atgtacaaat atcataaaaa aagagaatct 
      661 ttttaagcaa ggattttctt aacttcttcg gcgacagcat caccgacttc ggtggtactg 
      721 ttggaaccac ctaaatcacc agttctgata cctgcatcca aaaccttttt aactgcatct 
      781 tcaatggcct taccttcttc aggcaagttc aatgacaatt tcaacatcat tgcagcagac 
      841 aagatagtgg cgatagggtc aaccttattc tttggcaaat ctggagcaga accgtggcat 
      901 ggttcgtaca aaccaaatgc ggtgttcttg tctggcaaag aggccaagga cgcagatggc 
      961 aacaaaccca aggaacctgg gataacggag gcttcatcgg agatgatatc accaaacatg 
     1021 ttgctggtga ttataatacc atttaggtgg gttgggttct taactaggat catggcggca 
     1081 gaatcaatca attgatgttg aaccttcaat gtagggaatt cgttcttgat ggtttcctcc 
     1141 acagtttttc tccataatct tgaagaggcc aaaacattag ctttatccaa ggaccaaata 
     1201 ggcaatggtg gctcatgttg tagggccatg aaagcggcca ttcttgtgat tctttgcact 
     1261 tctggaacgg tgtattgttc actatcccaa gcgacaccat caccatcgtc ttcctttctc 
     1321 ttaccaaagt aaatacctcc cactaattct ctgacaacaa cgaagtcagt acctttagca 
     1381 aattgtggct tgattggaga taagtctaaa agagagtcgg atgcaaagtt acatggtctt 
     1441 aagttggcgt acaattgaag ttctttacgg atttttagta aaccttgttc aggtctaaca 
     1501 ctaccggtac cccatttagg accacccaca gcacctaaca aaacggcatc aaccttcttg 
     1561 gaggcttcca gcgcctcatc tggaagtggg acacctgtag catcgatagc agcaccacca 
     1621 attaaatgat tttcgaaatc gaacttgaca ttggaacgaa catcagaaat agctttaaga 
     1681 accttaatgg cttcggctgt gatttcttga ccaacgtggt cacctggcaa aacgacgatc 
     1741 ttcttagggg cagacatagg ggcagacatt agaatggtat atccttgaaa tatatatata 
     1801 tattgctgaa atgtaaaagg taagaaaagt tagaaagtaa gacgattgct aaccacctat 
     1861 tggaaaaaac aataggtcct taaataatat tgtcaacttc aagtattgtg atgcaagcat 
     1921 ttagtcatga acgcttctct attctatatg aaaagccggt tccggcctct cacctttcct 
     1981 ttttctccca atttttcagt tgaaaaaggt atatgcgtca ggcgacctct gaaattaaca 
     2041 aaaaatttcc agtcatcgaa tttgattctg tgcgatagcg cccctgtgtg ttctcgttat 
     2101 gttgaggaaa aaaataatgg ttgctaagag attcgaactc ttgcatctta cgatacctga 
     2161 gtattcccac agttaactgc ggtcaagata tttcttgaat caggcgcctt agaccgctcg 
     2221 gccaaacaac caattacttg ttgagaaata gagtataatt atcctataaa tataacgttt 
     2281 ttgaacacac atgaacaagg aagtacagga caattgattt tgaagagaat gtggattttg 
     2341 atgtaattgt tgggattcca tttttaataa ggcaataata ttaggtatgt ggatatacta 
     2401 gaagttctcc tcgaccgtcg atatgcggtg tgaaataccg cacagatgcg taaggagaaa 
     2461 ataccgcatc aggaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt 
     2521 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa 
     2581 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag 
     2641 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt 
     2701 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac 
     2761 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag 
     2821 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg 
     2881 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc gcgccattcg 
     2941 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc 
     3001 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc 
     3061 cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc 
     3121 gaattgggta ccgggccccc cctcgaggtc gacggtatcg ataagcttga tatcgaattc 
     3181 ctgcagcccg ggggatccac tagttctaga gcggccgcca ccgcggtgga gctccagctt 
     3241 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc 
     3301 tgtgtgaaat tgttatccgc tcacaattcc acacaacata ggagccggaa gcataaagtg 
     3361 taaagcctgg ggtgcctaat gagtgaggta actcacatta attgcgttgc gctcactgcc 
     3421 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 
     3481 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 
     3541 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 
     3601 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 
     3661 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 
     3721 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 
     3781 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 
     3841 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 
     3901 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 
     3961 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 
     4021 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 
     4081 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 
     4141 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 
     4201 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 
     4261 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 
     4321 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 
     4381 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 
     4441 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 
     4501 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 
     4561 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 
     4621 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 
     4681 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 
     4741 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 
     4801 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 
     4861 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 
     4921 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 
     4981 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 
     5041 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 
     5101 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 
     5161 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 
     5221 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 
     5281 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 
     5341 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 
     5401 aggggttccg cgcacatttc cccgaaaagt gccacctggg tccttttcat cacgtgctat 
     5461 aaaaataatt ataatttaaa ttttttaata taaatatata aattaaaaat agaaagtaaa 
     5521 aaaagaaatt aaagaaaaaa tagtttttgt tttccgaaga tgtaaaagac tctaggggga 
     5581 tcgccaacaa atactacctt ttatcttgct cttcctgctc tcaggtatta atgccgaatt 
     5641 gtttcatctt gtctgtgtag aagaccacac acgaaaatcc tgtgatttta cattttactt 
     5701 atcgttaatc gaatgtatat ctatttaatc tgcttttctt gtctaataaa tatatatgta 
     5761 aagtacgctt tttgttgaaa ttttttaaac ctttgtttat ttttttttct tcattccgta 
     5821 actcttctac cttctttatt tactttctaa aatccaaata caaaacataa aaataaataa 
     5881 acacagagta aattcccaaa ttattccatc attaaaagat acgaggcgcg tgtaagttac 
     5941 aggcaagcga tccgtcctaa gaaaccatta ttatcatgac attaacctat aaaaataggc 
     6001 gtatcacgag gccctttcgt c 
//
