LOCUS       pRS416                  4898 bp    DNA     circular     18-APR-2019
DEFINITION  LMBP 4060.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4060
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 18-APR-2019
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDBDATE|-12776405|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pRS416|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark-Zwijnaarde 71|
COMMENT     VNTOAUTHORAD3|B-9052 Gent Belgium|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTREPLTYPE|Phage
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     VNTEXTCHREPL|Yeast
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pRS416" 0 4898 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT        #1=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 2053 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 2 606 0 2049 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "HincII" "gtyrac" 4 0 3 924 0 1060 0 2028 0 0 
COMMENT            "") #4=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 2041 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 2071 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "ClaI" "atcgat" 3 0 1 2036 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 2090 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SacI" "gagctc" 6 0 1 2111 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 1 2102 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "SmaI" "cccggg" 4 0 1 2067 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "SpeI" "actagt" 2 0 1 2077 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 2083 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 2020 0 0 "")) 
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COMMENT        #15=(CFSignal (CObList) "lacZ fragment, incl. lacZalpha" 4 0 1 1808 2167 
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COMMENT        #16=(CFSignal (CObList) "f1 ori" 33 0 1 1351 1806 0 (CStringList) 
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COMMENT        #18=(CFSignal (CObList) "T3 promoter" 30 0 1 2126 2142 0 (CStringList) 
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COMMENT        #19=(CFSignal (CObList) "lac promoter" 30 0 1 2206 2289 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2206..2289)") 
COMMENT        #20=(CFSignal (CObList) "5' UTR of lacZ, incl. RBS" 32 0 1 2168 2205 0 
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COMMENT        #21=(CFSignal (CObList) "pMB1 ori" 33 0 1 2381 2783 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2381..2783)") 
COMMENT        #22=(CFSignal (CObList) "ampicillin resistance" 4 0 1 3324 4184 0 
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COMMENT        #23=(CFSignal (CObList) "CEN6/ARSH4 ori" 33 0 0 4320 4844 0 
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COMMENT        #25=(CFSignal (CObList) "LMBP 4060" 2 0 0 0 0 0 (CStringList) 
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COMMENT                          "DNA Plasmid Phage 'pRS416'" 1) 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 
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COMMENT                          "Creation Date: ? (no data)" 1) 
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COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 4898 bp" 1) 
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COMMENT                          "TEL: 0032-9-33-13-843  " 1) 
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COMMENT                          "EMAIL: <a href=\"mailto:bccm.genecorner@UGent.be\">bccm.genecorner@UGent.be</a>" 
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COMMENT                          "WWW: <a href=\"http://www.genecorner.ugent.be\">www.genecorner.ugent.be</a>" 
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FEATURES             Location/Qualifiers
     CDS             417..1220
                     /vntifkey="4"
                     /label=URA3
     CDS             complement(1808..2167)
                     /vntifkey="4"
                     /label=lacZ\fragment,\incl.\lacZalpha
     rep_origin      complement(1351..1806)
                     /vntifkey="33"
                     /label=f1\ori
     promoter        1977..1993
                     /vntifkey="30"
                     /label=T7\promoter
     promoter        complement(2126..2142)
                     /vntifkey="30"
                     /label=T3\promoter
     promoter        complement(2206..2289)
                     /vntifkey="30"
                     /label=lac\promoter
     RBS             complement(2168..2205)
                     /vntifkey="32"
                     /label=5'\UTR\of\lacZ,\incl.\RBS
     rep_origin      complement(2381..2783)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(3324..4184)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      4320..4844
                     /vntifkey="33"
                     /label=CEN6/ARSH4\ori
BASE COUNT     1362 a      1077 c      1167 g      1292 t 
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc 
      241 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg 
      301 agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc 
      361 cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt 
      421 cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat 
      481 ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca 
      541 aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg 
      601 tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg 
      661 ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca 
      721 aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac 
      781 acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa 
      841 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg 
      901 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct 
      961 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac 
     1021 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg 
     1081 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa 
     1141 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa 
     1201 gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac 
     1261 aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac 
     1321 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat 
     1381 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa 
     1441 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca 
     1501 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg 
     1561 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta 
     1621 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg 
     1681 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa 
     1741 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg 
     1801 gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg 
     1861 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg 
     1921 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat 
     1981 acgactcact atagggcgaa ttgggtaccg ggccccccct cgaggtcgac ggtatcgata 
     2041 agcttgatat cgaattcctg cagcccgggg gatccactag ttctagagcg gccgccaccg 
     2101 cggtggagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca 
     2161 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatagga 
     2221 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgaggtaact cacattaatt 
     2281 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga 
     2341 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc 
     2401 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 
     2461 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 
     2521 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 
     2581 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 
     2641 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 
     2701 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 
     2761 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 
     2821 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 
     2881 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 
     2941 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 
     3001 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 
     3061 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 
     3121 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 
     3181 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 
     3241 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 
     3301 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 
     3361 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 
     3421 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 
     3481 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 
     3541 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 
     3601 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc 
     3661 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 
     3721 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 
     3781 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 
     3841 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 
     3901 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca 
     3961 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 
     4021 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 
     4081 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 
     4141 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 
     4201 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 
     4261 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgggtcc 
     4321 ttttcatcac gtgctataaa aataattata atttaaattt tttaatataa atatataaat 
     4381 taaaaataga aagtaaaaaa agaaattaaa gaaaaaatag tttttgtttt ccgaagatgt 
     4441 aaaagactct agggggatcg ccaacaaata ctacctttta tcttgctctt cctgctctca 
     4501 ggtattaatg ccgaattgtt tcatcttgtc tgtgtagaag accacacacg aaaatcctgt 
     4561 gattttacat tttacttatc gttaatcgaa tgtatatcta tttaatctgc ttttcttgtc 
     4621 taataaatat atatgtaaag tacgcttttt gttgaaattt tttaaacctt tgtttatttt 
     4681 tttttcttca ttccgtaact cttctacctt ctttatttac tttctaaaat ccaaatacaa 
     4741 aacataaaaa taaataaaca cagagtaaat tcccaaatta ttccatcatt aaaagatacg 
     4801 aggcgcgtgt aagttacagg caagcgatcc gtcctaagaa accattatta tcatgacatt 
     4861 aacctataaa aataggcgta tcacgaggcc ctttcgtc 
//
