ID pRS416 standard; circular DNA; ; 4898 BP. DT 18-APR-2019 DE LMBP 4060. CC Accession number: LMBP 4060 CC (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium) CC Last modified: 18-APR-2019 CC This file is created by Vector NTI CC http://www.invitrogen.com/ CC ORIGDB|GenBank CC VNTDBDATE|-12776405| CC LSOWNER| CC VNTAUTHORNAME|Demo User| CC VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTOAUTHORTEL|0032-9-33-13-843| CC VNTOAUTHOREML|bccm.genecorner@UGent.be| CC VNTOAUTHORWWW|www.genecorner.ugent.be| CC VNTOAUTHORAD1|Ghent University| CC VNTOAUTHORAD2|Technologiepark-Zwijnaarde 71| CC VNTOAUTHORAD3|B-9052 Gent Belgium| CC VNTREPLTYPE|Plasmid CC VNTREPLTYPE|Phage CC VNTEXTCHREPL|Bacteria CC VNTEXTCHREPL|Yeast FH Key Location/Qualifiers FH FT CDS 417..1220 FT /vntifkey="4" FT /label=URA3 FT CDS complement(1808..2167) FT /vntifkey="4" FT /label=lacZ\fragment,\incl.\lacZalpha FT rep_origin complement(1351..1806) FT /vntifkey="33" FT /label=f1\ori FT promoter 1977..1993 FT /vntifkey="30" FT /label=T7\promoter FT promoter complement(2126..2142) FT /vntifkey="30" FT /label=T3\promoter FT promoter complement(2206..2289) FT /vntifkey="30" FT /label=lac\promoter FT RBS complement(2168..2205) FT /vntifkey="32" FT /label=5'\UTR\of\lacZ,\incl.\RBS FT rep_origin complement(2381..2783) FT /vntifkey="33" FT /label=pMB1\ori FT CDS complement(3324..4184) FT /vntifkey="4" FT /label=ampicillin\resistance FT rep_origin 4320..4844 FT /vntifkey="33" FT /label=CEN6/ARSH4\ori SQ Sequence 4898 BP; 1362 A; 1077 C; 1167 G; 1292 t; tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180 accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc 240 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg 300 agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc 360 cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt 420 cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat 480 ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca 540 aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg 600 tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg 660 ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca 720 aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac 780 acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa 840 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg 900 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct 960 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac 1020 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg 1080 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa 1140 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa 1200 gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac 1260 aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac 1320 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat 1380 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa 1440 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca 1500 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg 1560 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta 1620 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg 1680 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa 1740 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg 1800 gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg 1860 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg 1920 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat 1980 acgactcact atagggcgaa ttgggtaccg ggccccccct cgaggtcgac ggtatcgata 2040 agcttgatat cgaattcctg cagcccgggg gatccactag ttctagagcg gccgccaccg 2100 cggtggagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca 2160 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatagga 2220 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgaggtaact cacattaatt 2280 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga 2340 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc 2400 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 2460 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 2520 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 2580 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 2640 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 2700 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 2760 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 2820 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 2880 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 2940 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 3000 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 3060 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 3120 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 3180 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 3240 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 3300 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 3360 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 3420 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 3480 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 3540 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 3600 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc 3660 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 3720 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 3780 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 3840 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 3900 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca 3960 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 4020 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 4080 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 4140 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 4200 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 4260 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgggtcc 4320 ttttcatcac gtgctataaa aataattata atttaaattt tttaatataa atatataaat 4380 taaaaataga aagtaaaaaa agaaattaaa gaaaaaatag tttttgtttt ccgaagatgt 4440 aaaagactct agggggatcg ccaacaaata ctacctttta tcttgctctt cctgctctca 4500 ggtattaatg ccgaattgtt tcatcttgtc tgtgtagaag accacacacg aaaatcctgt 4560 gattttacat tttacttatc gttaatcgaa tgtatatcta tttaatctgc ttttcttgtc 4620 taataaatat atatgtaaag tacgcttttt gttgaaattt tttaaacctt tgtttatttt 4680 tttttcttca ttccgtaact cttctacctt ctttatttac tttctaaaat ccaaatacaa 4740 aacataaaaa taaataaaca cagagtaaat tcccaaatta ttccatcatt aaaagatacg 4800 aggcgcgtgt aagttacagg caagcgatcc gtcctaagaa accattatta tcatgacatt 4860 aacctataaa aataggcgta tcacgaggcc ctttcgtc 4898 //