LOCUS       pRS423                  5797 bp    DNA     circular     13-MAY-2014
DEFINITION  LMBP 4061.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4061
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 13-MAY-2014
COMMENT     VNTNAME|pRS423|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     VNTEXTCHREPL|Yeast
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pRS423" 0 5797 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 3452816845 3452816845 3452816845 
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COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
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COMMENT         "SacI" "SacII" "SalI" "SpeI" "XhoI" "XmaI" "XmnI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 5797 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 2143 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 3 829 0 1896 0 3590 0 
COMMENT            0 "") 
COMMENT        #2=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 2 4069 0 5001 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "ApaI" "gggccc" 6 0 1 2087 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 2125 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 2121 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "HincII" "gtyrac" 4 0 1 2100 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 2162 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SacI" "gagctc" 6 0 1 2183 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 1 2174 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "SalI" "gtcgac" 2 0 1 2098 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "SpeI" "actagt" 2 0 1 2149 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 2092 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "XmaI" "cccggg" 2 0 1 2137 0 0 "")) 
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COMMENT        #15=(CFSignal (CObList) "lacZ fragment, incl. lacZalpha" 4 0 1 1880 2239 
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COMMENT        #16=(CFSignal (CObList) "2 mu ori" 33 0 1 4388 5368 0 (CStringList) 
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COMMENT        #17=(CFSignal (CObList) "f1 ori" 33 0 1 1423 1878 0 (CStringList) 
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COMMENT        #18=(CFSignal (CObList) "T7 promoter" 30 0 0 2049 2065 0 (CStringList) 
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COMMENT        #19=(CFSignal (CObList) "T3 promoter" 30 0 1 2198 2214 0 (CStringList) 
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COMMENT        #20=(CFSignal (CObList) "lac promoter" 30 0 1 2278 2361 0 (CStringList) 
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COMMENT        #21=(CFSignal (CObList) "5' UTR of lacZ, incl. RBS" 32 0 1 2240 2277 0 
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COMMENT        #22=(CFSignal (CObList) "pMB1 ori" 33 0 1 2453 2855 0 (CStringList) 
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COMMENT        #23=(CFSignal (CObList) "ampicillin resistance" 4 0 1 3396 4256 0 
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COMMENT        #24=(CFSignal (CObList) "FLP terminator" 43 0 1 5446 5550 0 
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COMMENT                          "DNA Plasmid 'pRS423'" 1) 
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COMMENT                          "Creation Date: ? (no data)" 1) 
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FEATURES             Location/Qualifiers
     CDS             504..1163
                     /vntifkey="4"
                     /label=HIS3
                     /note="imidazoleglycerolphosphate dehydratase"
     CDS             complement(1880..2239)
                     /vntifkey="4"
                     /label=lacZ\fragment,\incl.\lacZalpha
     rep_origin      complement(4388..5368)
                     /vntifkey="33"
                     /label=2\mu\ori
     rep_origin      complement(1423..1878)
                     /vntifkey="33"
                     /label=f1\ori
     promoter        2049..2065
                     /vntifkey="30"
                     /label=T7\promoter
     promoter        complement(2198..2214)
                     /vntifkey="30"
                     /label=T3\promoter
     promoter        complement(2278..2361)
                     /vntifkey="30"
                     /label=lac\promoter
     RBS             complement(2240..2277)
                     /vntifkey="32"
                     /label=5'\UTR\of\lacZ,\incl.\RBS
     rep_origin      complement(2453..2855)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(3396..4256)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     terminator      complement(5446..5550)
                     /vntifkey="43"
                     /label=FLP\terminator
BASE COUNT     1536 a      1308 c      1374 g      1579 t 
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accataaatt cccgttttaa gagcttggtg agcgctagga gtcactgcca ggtatcgttt 
      241 gaacacggca ttagtcaggg aagtcataac acagtccttt cccgcaattt tctttttcta 
      301 ttactcttgg cctcctctag tacactctat atttttttat gcctcggtaa tgattttcat 
      361 tttttttttt cccctagcgg atgactcttt ttttttctta gcgattggca ttatcacata 
      421 atgaattata cattatataa agtaatgtga tttcttcgaa gaatatacta aaaaatgagc 
      481 aggcaagata aacgaaggca aagatgacag agcagaaagc cctagtaaag cgtattacaa 
      541 atgaaaccaa gattcagatt gcgatctctt taaagggtgg tcccctagcg atagagcact 
      601 cgatcttccc agaaaaagag gcagaagcag tagcagaaca ggccacacaa tcgcaagtga 
      661 ttaacgtcca cacaggtata gggtttctgg accatatgat acatgctctg gccaagcatt 
      721 ccggctggtc gctaatcgtt gagtgcattg gtgacttaca catagacgac catcacacca 
      781 ctgaagactg cgggattgct ctcggtcaag cttttaaaga ggccctactg gcgcgtggag 
      841 taaaaaggtt tggatcagga tttgcgcctt tggatgaggc actttccaga gcggtggtag 
      901 atctttcgaa caggccgtac gcagttgtcg aacttggttt gcaaagggag aaagtaggag 
      961 atctctcttg cgagatgatc ccgcattttc ttgaaagctt tgcagaggct agcagaatta 
     1021 ccctccacgt tgattgtctg cgaggcaaga atgatcatca ccgtagtgag agtgcgttca 
     1081 aggctcttgc ggttgccata agagaagcca cctcgcccaa tggtaccaac gatgttccct 
     1141 ccaccaaagg tgttcttatg tagtgacacc gattatttaa agctgcagca tacgatatat 
     1201 atacatgtgt atatatgtat acctatgaat gtcagtaagt atgtatacga acagtatgat 
     1261 actgaagatg acaaggtaat gcatcattct atacgtgtca ttctgaacga ggcgcgcttt 
     1321 ccttttttct ttttgctttt tctttttttt tctcttgaac tcgacggatc tatgcggtgt 
     1381 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta aacgttaata 
     1441 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg 
     1501 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc 
     1561 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa 
     1621 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt 
     1681 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac 
     1741 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta 
     1801 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg 
     1861 cgccgctaca gggcgcgtcg cgccattcgc cattcaggct gcgcaactgt tgggaagggc 
     1921 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc 
     1981 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg 
     2041 agcgcgcgta atacgactca ctatagggcg aattgggtac cgggcccccc ctcgaggtcg 
     2101 acggtatcga taagcttgat atcgaattcc tgcagcccgg gggatccact agttctagag 
     2161 cggccgccac cgcggtggag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc 
     2221 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca 
     2281 cacaacatag gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgaggtaa 
     2341 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag 
     2401 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc 
     2461 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 
     2521 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 
     2581 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 
     2641 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 
     2701 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 
     2761 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 
     2821 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 
     2881 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 
     2941 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 
     3001 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 
     3061 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc 
     3121 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 
     3181 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 
     3241 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 
     3301 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca 
     3361 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca 
     3421 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag 
     3481 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac 
     3541 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc 
     3601 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct 
     3661 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc 
     3721 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg 
     3781 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc 
     3841 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat 
     3901 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag 
     3961 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat 
     4021 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg 
     4081 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca 
     4141 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga 
     4201 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc 
     4261 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata 
     4321 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg 
     4381 ccacctgaac gaagcatctg tgcttcattt tgtagaacaa aaatgcaacg cgagagcgct 
     4441 aatttttcaa acaaagaatc tgagctgcat ttttacagaa cagaaatgca acgcgaaagc 
     4501 gctattttac caacgaagaa tctgtgcttc atttttgtaa aacaaaaatg caacgcgaga 
     4561 gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa atgcaacgcg 
     4621 agagcgctat tttaccaaca aagaatctat acttcttttt tgttctacaa aaatgcatcc 
     4681 cgagagcgct atttttctaa caaagcatct tagattactt tttttctcct ttgtgcgctc 
     4741 tataatgcag tctcttgata actttttgca ctgtaggtcc gttaaggtta gaagaaggct 
     4801 actttggtgt ctattttctc ttccataaaa aaagcctgac tccacttccc gcgtttactg 
     4861 attactagcg aagctgcggg tgcatttttt caagataaag gcatccccga ttatattcta 
     4921 taccgatgtg gattgcgcat actttgtgaa cagaaagtga tagcgttgat gattcttcat 
     4981 tggtcagaaa attatgaacg gtttcttcta ttttgtctct atatactacg tataggaaat 
     5041 gtttacattt tcgtattgtt ttcgattcac tctatgaata gttcttacta caattttttt 
     5101 gtctaaagag taatactaga gataaacata aaaaatgtag aggtcgagtt tagatgcaag 
     5161 ttcaaggagc gaaaggtgga tgggtaggtt atatagggat atagcacaga gatatatagc 
     5221 aaagagatac ttttgagcaa tgtttgtgga agcggtattc gcaatatttt agtagctcgt 
     5281 tacagtccgg tgcgtttttg gttttttgaa agtgcgtctt cagagcgctt ttggttttca 
     5341 aaagcgctct gaagttccta tactttctag agaataggaa cttcggaata ggaacttcaa 
     5401 agcgtttccg aaaacgagcg cttccgaaaa tgcaacgcga gctgcgcaca tacagctcac 
     5461 tgttcacgtc gcacctatat ctgcgtgttg cctgtatata tatatacatg agaagaacgg 
     5521 catagtgcgt gtttatgctt aaatgcgtac ttatatgcgt ctatttatgt aggatgaaag 
     5581 gtagtctagt acctcctgtg atattatccc attccatgcg gggtatcgta tgcttccttc 
     5641 agcactaccc tttagctgtt ctatatgctg ccactcctca attggattag tctcatcctt 
     5701 caatgctatc atttcctttg atattggatc atctaagaaa ccattattat catgacatta 
     5761 acctataaaa ataggcgtat cacgaggccc tttcgtc 
//
