ID pRS423 standard; circular DNA; ; 5797 BP. DE LMBP 4061. CC Accession number: LMBP 4061 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 13-MAY-2014 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT CDS 504..1163 FT /vntifkey="4" FT /label=HIS3 FT /note="imidazoleglycerolphosphate dehydratase" FT CDS complement(1880..2239) FT /vntifkey="4" FT /label=lacZ\fragment,\incl.\lacZalpha FT rep_origin complement(4388..5368) FT /vntifkey="33" FT /label=2\mu\ori FT rep_origin complement(1423..1878) FT /vntifkey="33" FT /label=f1\ori FT promoter 2049..2065 FT /vntifkey="30" FT /label=T7\promoter FT promoter complement(2198..2214) FT /vntifkey="30" FT /label=T3\promoter FT promoter complement(2278..2361) FT /vntifkey="30" FT /label=lac\promoter FT RBS complement(2240..2277) FT /vntifkey="32" FT /label=5'\UTR\of\lacZ,\incl.\RBS FT rep_origin complement(2453..2855) FT /vntifkey="33" FT /label=pMB1\ori FT CDS complement(3396..4256) FT /vntifkey="4" FT /label=ampicillin\resistance FT terminator complement(5446..5550) FT /vntifkey="43" FT /label=FLP\terminator SQ Sequence 5797 BP; 1536 A; 1308 C; 1374 G; 1579 t; tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180 accataaatt cccgttttaa gagcttggtg agcgctagga gtcactgcca ggtatcgttt 240 gaacacggca ttagtcaggg aagtcataac acagtccttt cccgcaattt tctttttcta 300 ttactcttgg cctcctctag tacactctat atttttttat gcctcggtaa tgattttcat 360 tttttttttt cccctagcgg atgactcttt ttttttctta gcgattggca ttatcacata 420 atgaattata cattatataa agtaatgtga tttcttcgaa gaatatacta aaaaatgagc 480 aggcaagata aacgaaggca aagatgacag agcagaaagc cctagtaaag cgtattacaa 540 atgaaaccaa gattcagatt gcgatctctt taaagggtgg tcccctagcg atagagcact 600 cgatcttccc agaaaaagag gcagaagcag tagcagaaca ggccacacaa tcgcaagtga 660 ttaacgtcca cacaggtata gggtttctgg accatatgat acatgctctg gccaagcatt 720 ccggctggtc gctaatcgtt gagtgcattg gtgacttaca catagacgac catcacacca 780 ctgaagactg cgggattgct ctcggtcaag cttttaaaga ggccctactg gcgcgtggag 840 taaaaaggtt tggatcagga tttgcgcctt tggatgaggc actttccaga gcggtggtag 900 atctttcgaa caggccgtac gcagttgtcg aacttggttt gcaaagggag aaagtaggag 960 atctctcttg cgagatgatc ccgcattttc ttgaaagctt tgcagaggct agcagaatta 1020 ccctccacgt tgattgtctg cgaggcaaga atgatcatca ccgtagtgag agtgcgttca 1080 aggctcttgc ggttgccata agagaagcca cctcgcccaa tggtaccaac gatgttccct 1140 ccaccaaagg tgttcttatg tagtgacacc gattatttaa agctgcagca tacgatatat 1200 atacatgtgt atatatgtat acctatgaat gtcagtaagt atgtatacga acagtatgat 1260 actgaagatg acaaggtaat gcatcattct atacgtgtca ttctgaacga ggcgcgcttt 1320 ccttttttct ttttgctttt tctttttttt tctcttgaac tcgacggatc tatgcggtgt 1380 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta aacgttaata 1440 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg 1500 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc 1560 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa 1620 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt 1680 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac 1740 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta 1800 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg 1860 cgccgctaca gggcgcgtcg cgccattcgc cattcaggct gcgcaactgt tgggaagggc 1920 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc 1980 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg 2040 agcgcgcgta atacgactca ctatagggcg aattgggtac cgggcccccc ctcgaggtcg 2100 acggtatcga taagcttgat atcgaattcc tgcagcccgg gggatccact agttctagag 2160 cggccgccac cgcggtggag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc 2220 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca 2280 cacaacatag gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgaggtaa 2340 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag 2400 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc 2460 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 2520 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 2580 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 2640 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 2700 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 2760 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 2820 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 2880 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 2940 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 3000 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 3060 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc 3120 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 3180 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 3240 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 3300 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca 3360 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca 3420 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag 3480 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac 3540 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc 3600 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct 3660 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc 3720 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg 3780 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc 3840 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat 3900 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag 3960 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat 4020 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg 4080 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca 4140 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga 4200 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc 4260 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata 4320 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg 4380 ccacctgaac gaagcatctg tgcttcattt tgtagaacaa aaatgcaacg cgagagcgct 4440 aatttttcaa acaaagaatc tgagctgcat ttttacagaa cagaaatgca acgcgaaagc 4500 gctattttac caacgaagaa tctgtgcttc atttttgtaa aacaaaaatg caacgcgaga 4560 gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa atgcaacgcg 4620 agagcgctat tttaccaaca aagaatctat acttcttttt tgttctacaa aaatgcatcc 4680 cgagagcgct atttttctaa caaagcatct tagattactt tttttctcct ttgtgcgctc 4740 tataatgcag tctcttgata actttttgca ctgtaggtcc gttaaggtta gaagaaggct 4800 actttggtgt ctattttctc ttccataaaa aaagcctgac tccacttccc gcgtttactg 4860 attactagcg aagctgcggg tgcatttttt caagataaag gcatccccga ttatattcta 4920 taccgatgtg gattgcgcat actttgtgaa cagaaagtga tagcgttgat gattcttcat 4980 tggtcagaaa attatgaacg gtttcttcta ttttgtctct atatactacg tataggaaat 5040 gtttacattt tcgtattgtt ttcgattcac tctatgaata gttcttacta caattttttt 5100 gtctaaagag taatactaga gataaacata aaaaatgtag aggtcgagtt tagatgcaag 5160 ttcaaggagc gaaaggtgga tgggtaggtt atatagggat atagcacaga gatatatagc 5220 aaagagatac ttttgagcaa tgtttgtgga agcggtattc gcaatatttt agtagctcgt 5280 tacagtccgg tgcgtttttg gttttttgaa agtgcgtctt cagagcgctt ttggttttca 5340 aaagcgctct gaagttccta tactttctag agaataggaa cttcggaata ggaacttcaa 5400 agcgtttccg aaaacgagcg cttccgaaaa tgcaacgcga gctgcgcaca tacagctcac 5460 tgttcacgtc gcacctatat ctgcgtgttg cctgtatata tatatacatg agaagaacgg 5520 catagtgcgt gtttatgctt aaatgcgtac ttatatgcgt ctatttatgt aggatgaaag 5580 gtagtctagt acctcctgtg atattatccc attccatgcg gggtatcgta tgcttccttc 5640 agcactaccc tttagctgtt ctatatgctg ccactcctca attggattag tctcatcctt 5700 caatgctatc atttcctttg atattggatc atctaagaaa ccattattat catgacatta 5760 acctataaaa ataggcgtat cacgaggccc tttcgtc 5797 //