ID pRS425; SV 1; circular; unknown; STD; UNC; 6849 BP. AC unknown; DT 19-JAN.-2021 DE LMBP 4063. OS unknown CC Accession number: LMBP 4063 CC (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium) CC Last modified: 19-JAN-2021 FH Key Location/Qualifiers FH FT CDS complement(663..1757) FT /label="LEU2" FT rep_origin complement(2474..2929) FT /label="f1 ori" FT CDS complement(2931..3290) FT /label="lacZ fragment, incl. lacZalpha" FT promoter 3100..3116 FT /label="T7 promoter" FT promoter complement(3249..3265) FT /label="T3 promoter" FT RBS complement(3291..3328) FT /label="5' UTR of lacZ, incl. RBS" FT promoter complement(3329..3412) FT /label="lac promoter" FT rep_origin complement(3504..3906) FT /label="pMB1 ori" FT CDS complement(4447..5307) FT /label="ampicillin resistance" FT rep_origin complement(5439..6419) FT /label="2mu ori" FT terminator complement(6497..6601) FT /label="FLP terminator" SQ SEQUENCE 6849 AA; 2116284 MW; TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA 60 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG 120 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC 180 ACCATATCGA CTACGTCGTA AGGCCGTTTC TGACAGAGTA AAATTCTTGA GGGAACTTTC 240 ACCATTATGG GAAATGCTTC AAGAAGGTAT TGACTTAAAC TCCATCAAAT GGTCAGGTCA 300 TTGAGTGTTT TTTATTTGTT GTATTTTTTT TTTTTTAGAG AAAATCCTCC AATATCAAAT 360 TAGGAATCGT AGTTTCATGA TTTTCTGTTA CACCTAACTT TTTGTGTGGT GCCCTCCTCC 420 TTGTCAATAT TAATGTTAAA GTGCAATTCT TTTTCCTTAT CACGTTGAGC CATTAGTATC 480 AATTTGCTTA CCTGTATTCC TTTACTATCC TCCTTTTTCT CCTTCTTGAT AAATGTATGT 540 AGATTGCGTA TATAGTTTCG TCTACCCTAT GAACATATTC CATTTTGTAA TTTCGTGTCG 600 TTTCTATTAT GAATTTCATT TATAAAGTTT ATGTACAAAT ATCATAAAAA AAGAGAATCT 660 TTTTAAGCAA GGATTTTCTT AACTTCTTCG GCGACAGCAT CACCGACTTC GGTGGTACTG 720 TTGGAACCAC CTAAATCACC AGTTCTGATA CCTGCATCCA AAACCTTTTT AACTGCATCT 780 TCAATGGCCT TACCTTCTTC AGGCAAGTTC AATGACAATT TCAACATCAT TGCAGCAGAC 840 AAGATAGTGG CGATAGGGTC AACCTTATTC TTTGGCAAAT CTGGAGCAGA ACCGTGGCAT 900 GGTTCGTACA AACCAAATGC GGTGTTCTTG TCTGGCAAAG AGGCCAAGGA CGCAGATGGC 960 AACAAACCCA AGGAACCTGG GATAACGGAG GCTTCATCGG AGATGATATC ACCAAACATG 1020 TTGCTGGTGA TTATAATACC ATTTAGGTGG GTTGGGTTCT TAACTAGGAT CATGGCGGCA 1080 GAATCAATCA ATTGATGTTG AACCTTCAAT GTAGGGAATT CGTTCTTGAT GGTTTCCTCC 1140 ACAGTTTTTC TCCATAATCT TGAAGAGGCC AAAAGATTAG CTTTATCCAA GGACCAAATA 1200 GGCAATGGTG GCTCATGTTG TAGGGCCATG AAAGCGGCCA TTCTTGTGAT TCTTTGCACT 1260 TCTGGAACGG TGTATTGTTC ACTATCCCAA GCGACACCAT CACCATCGTC TTCCTTTCTC 1320 TTACCAAAGT AAATACCTCC CACTAATTCT CTGACAACAA CGAAGTCAGT ACCTTTAGCA 1380 AATTGTGGCT TGATTGGAGA TAAGTCTAAA AGAGAGTCGG ATGCAAAGTT ACATGGTCTT 1440 AAGTTGGCGT ACAATTGAAG TTCTTTACGG ATTTTTAGTA AACCTTGTTC AGGTCTAACA 1500 CTACCGGTAC CCCATTTAGG ACCAGCCACA GCACCTAACA AAACGGCATC AACCTTCTTG 1560 GAGGCTTCCA GCGCCTCATC TGGAAGTGGG ACACCTGTAG CATCGATAGC AGCACCACCA 1620 ATTAAATGAT TTTCGAAATC GAACTTGACA TTGGAACGAA CATCAGAAAT AGCTTTAAGA 1680 ACCTTAATGG CTTCGGCTGT GATTTCTTGA CCAACGTGGT CACCTGGCAA AACGACGATC 1740 TTCTTAGGGG CAGACATAGG GGCAGACATT AGAATGGTAT ATCCTTGAAA TATATATATA 1800 TATTGCTGAA ATGTAAAAGG TAAGAAAAGT TAGAAAGTAA GACGATTGCT AACCACCTAT 1860 TGGAAAAAAC AATAGGTCCT TAAATAATAT TGTCAACTTC AAGTATTGTG ATGCAAGCAT 1920 TTAGTCATGA ACGCTTCTCT ATTCTATATG AAAAGCCGGT TCCGGCCTCT CACCTTTCCT 1980 TTTTCTCCCA ATTTTTCAGT TGAAAAAGGT ATATGCGTCA GGCGACCTCT GAAATTAACA 2040 AAAAATTTCC AGTCATCGAA TTTGATTCTG TGCGATAGCG CCCCTGTGTG TTCTCGTTAT 2100 GTTGAGGAAA AAAATAATGG TTGCTAAGAG ATTCGAACTC TTGCATCTTA CGATACCTGA 2160 GTATTCCCAC AGTTAACTGC GGTCAAGATA TTTCTTGAAT CAGGCGCCTT AGACCGCTCG 2220 GCCAAACAAC CAATTACTTG TTGAGAAATA GAGTATAATT ATCCTATAAA TATAACGTTT 2280 TTGAACACAC ATGAACAAGG AAGTACAGGA CAATTGATTT TGAAGAGAAT GTGGATTTTG 2340 ATGTAATTGT TGGGATTCCA TTTTTAATAA GGCAATAATA TTAGGTATGT GGATATACTA 2400 GAAGTTCTCC TCGACCGTCG ATATGCGGTG TGAAATACCG CACAGATGCG TAAGGAGAAA 2460 ATACCGCATC AGGAAATTGT AAACGTTAAT ATTTTGTTAA AATTCGCGTT AAATTTTTGT 2520 TAAATCAGCT CATTTTTTAA CCAATAGGCC GAAATCGGCA AAATCCCTTA TAAATCAAAA 2580 GAATAGACCG AGATAGGGTT GAGTGTTGTT CCAGTTTGGA ACAAGAGTCC ACTATTAAAG 2640 AACGTGGACT CCAACGTCAA AGGGCGAAAA ACCGTCTATC AGGGCGATGG CCCACTACGT 2700 GAACCATCAC CCTAATCAAG TTTTTTGGGG TCGAGGTGCC GTAAAGCACT AAATCGGAAC 2760 CCTAAAGGGA GCCCCCGATT TAGAGCTTGA CGGGGAAAGC CGGCGAACGT GGCGAGAAAG 2820 GAAGGGAAGA AAGCGAAAGG AGCGGGCGCT AGGGCGCTGG CAAGTGTAGC GGTCACGCTG 2880 CGCGTAACCA CCACACCCGC CGCGCTTAAT GCGCCGCTAC AGGGCGCGTC GCGCCATTCG 2940 CCATTCAGGC TGCGCAACTG TTGGGAAGGG CGATCGGTGC GGGCCTCTTC GCTATTACGC 3000 CAGCTGGCGA AAGGGGGATG TGCTGCAAGG CGATTAAGTT GGGTAACGCC AGGGTTTTCC 3060 CAGTCACGAC GTTGTAAAAC GACGGCCAGT GAGCGCGCGT AATACGACTC ACTATAGGGC 3120 GAATTGGGTA CCGGGCCCCC CCTCGAGGTC GACGGTATCG ATAAGCTTGA TATCGAATTC 3180 CTGCAGCCCG GGGGATCCAC TAGTTCTAGA GCGGCCGCCA CCGCGGTGGA GCTCCAGCTT 3240 TTGTTCCCTT TAGTGAGGGT TAATTGCGCG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC 3300 TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA GGAGCCGGAA GCATAAAGTG 3360 TAAAGCCTGG GGTGCCTAAT GAGTGAGGTA ACTCACATTA ATTGCGTTGC GCTCACTGCC 3420 CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG 3480 GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC 3540 GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 3600 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 3660 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 3720 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 3780 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 3840 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 3900 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 3960 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 4020 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 4080 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG 4140 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 4200 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 4260 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 4320 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 4380 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC 4440 TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC 4500 ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC 4560 TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC 4620 AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC 4680 CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT 4740 GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC 4800 TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA 4860 AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT 4920 ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG 4980 CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC 5040 GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA 5100 AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT 5160 GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT 5220 CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG 5280 GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA 5340 TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT 5400 AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAA CGAAGCATCT GTGCTTCATT 5460 TTGTAGAACA AAAATGCAAC GCGAGAGCGC TAATTTTTCA AACAAAGAAT CTGAGCTGCA 5520 TTTTTACAGA ACAGAAATGC AACGCGAAAG CGCTATTTTA CCAACGAAGA ATCTGTGCTT 5580 CATTTTTGTA AAACAAAAAT GCAACGCGAG AGCGCTAATT TTTCAAACAA AGAATCTGAG 5640 CTGCATTTTT ACAGAACAGA AATGCAACGC GAGAGCGCTA TTTTACCAAC AAAGAATCTA 5700 TACTTCTTTT TTGTTCTACA AAAATGCATC CCGAGAGCGC TATTTTTCTA ACAAAGCATC 5760 TTAGATTACT TTTTTTCTCC TTTGTGCGCT CTATAATGCA GTCTCTTGAT AACTTTTTGC 5820 ACTGTAGGTC CGTTAAGGTT AGAAGAAGGC TACTTTGGTG TCTATTTTCT CTTCCATAAA 5880 AAAAGCCTGA CTCCACTTCC CGCGTTTACT GATTACTAGC GAAGCTGCGG GTGCATTTTT 5940 TCAAGATAAA GGCATCCCCG ATTATATTCT ATACCGATGT GGATTGCGCA TACTTTGTGA 6000 ACAGAAAGTG ATAGCGTTGA TGATTCTTCA TTGGTCAGAA AATTATGAAC GGTTTCTTCT 6060 ATTTTGTCTC TATATACTAC GTATAGGAAA TGTTTACATT TTCGTATTGT TTTCGATTCA 6120 CTCTATGAAT AGTTCTTACT ACAATTTTTT TGTCTAAAGA GTAATACTAG AGATAAACAT 6180 AAAAAATGTA GAGGTCGAGT TTAGATGCAA GTTCAAGGAG CGAAAGGTGG ATGGGTAGGT 6240 TATATAGGGA TATAGCACAG AGATATATAG CAAAGAGATA CTTTTGAGCA ATGTTTGTGG 6300 AAGCGGTATT CGCAATATTT TAGTAGCTCG TTACAGTCCG GTGCGTTTTT GGTTTTTTGA 6360 AAGTGCGTCT TCAGAGCGCT TTTGGTTTTC AAAAGCGCTC TGAAGTTCCT ATACTTTCTA 6420 GAGAATAGGA ACTTCGGAAT AGGAACTTCA AAGCGTTTCC GAAAACGAGC GCTTCCGAAA 6480 ATGCAACGCG AGCTGCGCAC ATACAGCTCA CTGTTCACGT CGCACCTATA TCTGCGTGTT 6540 GCCTGTATAT ATATATACAT GAGAAGAACG GCATAGTGCG TGTTTATGCT TAAATGCGTA 6600 CTTATATGCG TCTATTTATG TAGGATGAAA GGTAGTCTAG TACCTCCTGT GATATTATCC 6660 CATTCCATGC GGGGTATCGT ATGCTTCCTT CAGCACTACC CTTTAGCTGT TCTATATGCT 6720 GCCACTCCTC AATTGGATTA GTCTCATCCT TCAATGCTAT CATTTCCTTT GATATTGGAT 6780 CATACTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC 6840 CCTTTCGTC 6849 //